Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,920,809 G→A 15.8% P27L (CCG→CTG)  atpF ← F0 sector of membrane‑bound ATP synthase, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,920,8090GA15.8% 368.6 / 51.7 161P27L (CCG→CTG) atpFF0 sector of membrane‑bound ATP synthase, subunit b
Reads supporting (aligned to +/- strand):  ref base G (62/73);  new base A (12/14);  total (74/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-02

Only 100 of 174 total aligned reads displayed.

CTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAG  >  NC_000913/3920710‑3920908
                                                                                                   |                                                                                                   
cTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcg                                                                                                     <  2:2551364/100‑1 (MQ=255)
  ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                   <  1:892100/100‑1 (MQ=255)
  ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                   >  2:105058/1‑100 (MQ=255)
  ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                   <  1:2654985/100‑1 (MQ=255)
  ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATggcagc                                                                                                   <  1:277969/100‑1 (MQ=255)
    ccTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCa                                                                                                 >  1:1092884/1‑100 (MQ=255)
     cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGCTGGCTGCCATTAATGGCGGCCAt                                                                                                >  2:1411184/1‑100 (MQ=255)
     cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCAt                                                                                                <  1:187585/100‑1 (MQ=255)
     cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCAt                                                                                                >  2:2345900/1‑100 (MQ=255)
     cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                                <  2:291544/100‑1 (MQ=255)
     cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                                <  2:1735490/100‑1 (MQ=255)
     atctGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCAt                                                                                                <  2:110792/97‑1 (MQ=255)
      tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATa                                                                                               >  1:59745/1‑100 (MQ=255)
      tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                               <  1:1522978/100‑2 (MQ=255)
      tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                               <  2:34389/100‑2 (MQ=255)
      tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                               <  1:2269520/100‑2 (MQ=255)
      tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                               <  1:2035068/100‑2 (MQ=255)
      tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                               <  2:1263784/100‑2 (MQ=255)
      tttGCAAGGTCAAGGTCCTTATGTCCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCTATGGCCGTCATTAATGGCAGCCAtc                                                                                               <  2:2072058/100‑2 (MQ=255)
         gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAATCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtcgaa                                                                                            <  1:914371/100‑5 (MQ=38)
         gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                                            <  2:2419905/100‑1 (MQ=255)
         gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                                            >  1:467775/1‑100 (MQ=255)
          caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                                           >  1:2395695/1‑100 (MQ=255)
             ggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                                        >  2:1457960/1‑100 (MQ=255)
              gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTc                                                                                       >  1:307775/1‑100 (MQ=255)
              gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTc                                                                                       <  1:2870921/100‑1 (MQ=255)
              gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTAATTc                                                                                       >  1:2659722/1‑100 (MQ=255)
               tcaaggtcCTTATGTGCTCGTTTTGTCGTAGCAAGGTCTTTAGCAATTTCTTTTTGACTTTTTTCTATGGCTGCCATTATTGGCGGCAATACGTACtt                                                                                        <  2:745372/98‑1 (MQ=255)
                caaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                                     >  1:2588823/1‑100 (MQ=255)
                caaggtcCTTATGGGCTCGTTCTGCGGAAGCAGGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGTCATTAATGGCAGCCAtcgaaaaacg                                                                                       <  2:1359513/98‑10 (MQ=25)
                 aaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATg                                                                                    <  1:2410087/100‑1 (MQ=255)
                  aggtcCTTATGTGCTCGTTCTGCGGAATCAAGGTCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                                   <  2:638705/100‑1 (MQ=255)
                   ggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGca                                                                                  >  1:367265/1‑100 (MQ=255)
                    gtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                                 <  1:1587838/100‑1 (MQ=255)
                     tcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcaga                                                                                <  1:2345927/100‑1 (MQ=255)
                     tcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcaga                                                                                <  1:111889/100‑1 (MQ=255)
                         tttgtgATCGTTCTGCGGAAGCAAGGCCGTGAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCTATACGTACTTCATGcagaacag                                                                            <  2:2744928/98‑1 (MQ=255)
                         tATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacag                                                                            <  1:137317/100‑1 (MQ=255)
                         tATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATAGCGGCCATACGTACTTCATGcagaacag                                                                            >  2:2416091/1‑100 (MQ=255)
                           tgtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacagaa                                                                          >  2:2915950/1‑100 (MQ=255)
                           tgtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacagaa                                                                          >  1:2978866/1‑100 (MQ=255)
                            gtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCagaacagaac                                                                         <  1:1809745/100‑1 (MQ=255)
                              gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacga                                                                       <  2:167707/100‑1 (MQ=255)
                              gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacga                                                                       >  2:1204033/1‑100 (MQ=255)
                              gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTAACATTAATGGCAACCATACGTACTTCATGCAGAACAgaacga                                                                       >  1:1041352/1‑100 (MQ=255)
                                 cGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACa                                                                    <  1:2233372/100‑1 (MQ=255)
                                 cGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACa                                                                    <  1:1204044/100‑1 (MQ=255)
                                  gTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAg                                                                   <  2:1126911/100‑1 (MQ=255)
                                   ttCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAgg                                                                  <  2:2588791/100‑1 (MQ=255)
                                      tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACTTTTTTCGATGGCTGCCATTAATTGCGGCCATACGTACTTCATGTAGAACAGAACGAACAGGACa                                                               <  2:358121/100‑1 (MQ=255)
                                         ggAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAc                                                            >  2:2851053/1‑100 (MQ=255)
                                               aaGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtg                                                      >  2:2209564/1‑100 (MQ=255)
                                                aGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtgg                                                     <  2:823727/100‑1 (MQ=255)
                                                aGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtgg                                                     >  2:2747874/1‑100 (MQ=255)
                                                   ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcct                                                  >  2:763221/1‑100 (MQ=255)
                                                   ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcct                                                  <  2:2195331/100‑1 (MQ=255)
                                                    cGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctg                                                 <  1:1541224/100‑1 (MQ=255)
                                                        aGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtgg                                                     >  2:2974438/1‑92 (MQ=255)
                                                        aGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCg                                             >  1:2661575/1‑100 (MQ=255)
                                                         gCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGa                                            >  2:419830/1‑100 (MQ=255)
                                                           aaTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAgg                                          <  2:2157103/100‑1 (MQ=255)
                                                            aTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGa                                         >  2:189424/1‑100 (MQ=255)
                                                             tttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAt                                        <  1:2411154/100‑1 (MQ=255)
                                                               tCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttg                                      >  2:1287277/1‑100 (MQ=255)
                                                               tCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttg                                      <  1:1557927/100‑1 (MQ=255)
                                                                 tttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgtt                                    <  1:176886/100‑1 (MQ=255)
                                                                   tttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGc                                  <  1:1243035/100‑1 (MQ=255)
                                                                      gACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt                               <  2:547483/100‑1 (MQ=255)
                                                                      gACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt                               >  2:1986875/1‑100 (MQ=255)
                                                                      gACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt                               >  1:2936873/1‑100 (MQ=255)
                                                                       aCGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                              <  1:1019896/100‑1 (MQ=255)
                                                                        cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa                             <  2:467772/100‑1 (MQ=255)
                                                                        cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa                             >  1:1497812/1‑100 (MQ=255)
                                                                        cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa                             <  2:822241/100‑1 (MQ=255)
                                                                        cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa                             <  1:1890434/100‑1 (MQ=255)
                                                                         gTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAAAAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAg                            <  1:562575/100‑1 (MQ=255)
                                                                          tttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa                           >  1:1211856/1‑100 (MQ=255)
                                                                             acgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTc                        >  2:1718058/5‑100 (MQ=39)
                                                                             acgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTc                        >  2:2391398/5‑100 (MQ=39)
                                                                              tCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTca                       <  2:1868553/100‑1 (MQ=255)
                                                                              cgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATTGCCTGGCCGAGGATTGTTGCGTTAAGATTca                       >  1:99891/4‑100 (MQ=39)
                                                                               cGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcac                      <  2:269004/100‑1 (MQ=255)
                                                                               cGATGGCTGCCATTAATGGCGGACATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcac                      <  2:2777280/100‑1 (MQ=255)
                                                                                taTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGAAAAACGCGATGGCATGGCCGAGGATTGTTGCGTTAAGATTcaca                     >  1:1747155/2‑100 (MQ=255)
                                                                                  tGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGc                   >  2:1574127/1‑100 (MQ=255)
                                                                                  tGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGc                   >  2:608225/1‑100 (MQ=255)
                                                                                  tGGCCGCCATTAATGGCAGCAATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGc                   >  2:1499660/1‑100 (MQ=255)
                                                                                      tGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCTAGGATTGTTGCGTTAAGATTCACAGCACaa               >  1:1148487/1‑100 (MQ=255)
                                                                                        ccATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATg             <  1:1251747/100‑1 (MQ=255)
                                                                                        ccATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATg             >  1:1942228/1‑100 (MQ=255)
                                                                                         cATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGc            >  1:476833/1‑100 (MQ=255)
                                                                                          aTTAATGGCGCCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGTGGGTTGTTGCGTTAAGATTCACAGCACAATGcn           <  2:1992585/100‑2 (MQ=255)
                                                                                           ttAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCct          >  2:852218/1‑100 (MQ=255)
                                                                                           ttAATGGCGGCAATACGTACTTCATGCAGAACAGATCGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGcc           <  2:2579174/99‑1 (MQ=255)
                                                                                              aTGGCGGCCATACGTACTTCATGCAGAACAGAAGGAATAGGAAAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGTTTCACAGCACAATGCctct        <  2:243812/99‑1 (MQ=255)
                                                                                              aTGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTa       >  1:1474956/1‑100 (MQ=255)
                                                                                              aTGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTa       >  1:1475317/1‑100 (MQ=255)
                                                                                                ggtggcCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAGTGTGGCGTTAAGATTCACAGCACAATGCCTCTAtt     >  2:892754/4‑100 (MQ=255)
                                                                                                  cggcCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTa   <  1:1457975/100‑1 (MQ=255)
                                                                                                   ggcCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAg  >  1:1699704/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAG  >  NC_000913/3920710‑3920908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: