Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,177,171 T→A 16.8% intergenic (+43/‑187) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,177,1710TA16.8% 223.6 / 40.5 102intergenic (+43/‑187)tufB/secEtranslation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (40/44);  new base A (8/9);  total (48/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01

Only 100 of 114 total aligned reads displayed.

ATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATA  >  NC_000913/4177072‑4177269
                                                                                                   |                                                                                                  
aTCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGcatcat                                                                                                    >  2:860079/1‑100 (MQ=255)
aTCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGcatcat                                                                                                    <  2:1380713/100‑1 (MQ=255)
 tCCGTGAAGGCGGCCGTACCGATGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCCATAACATTTGACGCAATGCGCACTAAAAGGGGATCAtt                                                                                                   >  2:1891937/1‑100 (MQ=255)
  ccGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGATCTAATTGCTGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAttt                                                                                                  >  2:538049/1‑100 (MQ=255)
     tGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGAt                                                                                               >  1:2123902/1‑100 (MQ=255)
      gAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATg                                                                                              <  2:1159842/100‑1 (MQ=255)
       aaGGCGGCCGTACCGTTGGCTCGGGCTTTGTATGAAAAGTTCTTAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATg                                                                                              <  1:1307333/99‑1 (MQ=38)
       aaGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGc                                                                                             <  1:745795/100‑1 (MQ=255)
       aaGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGc                                                                                             <  1:2209952/100‑1 (MQ=255)
       aaGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATACACACTAAAAAGGCATAAtttaa                                                                                                >  1:113997/1‑95 (MQ=25)
         ggcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCTGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGccc                                                                                           >  2:1745712/1‑100 (MQ=255)
         ggcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGccc                                                                                           <  2:402532/100‑1 (MQ=255)
         ggcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGccc                                                                                           <  1:1982361/100‑1 (MQ=255)
           cggcCGTACCGTTGGCGCGGGCGTTGTATCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCt                                                                                          <  1:532631/99‑1 (MQ=38)
           cggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCtt                                                                                         >  1:2011046/1‑100 (MQ=255)
           cggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtt                                                                                         <  2:1698944/100‑1 (MQ=255)
             gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTTAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                       <  1:141557/100‑1 (MQ=38)
             gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCtttt                                                                                       >  2:2479837/1‑100 (MQ=255)
             gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                       <  2:1098974/100‑1 (MQ=255)
              ccGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttttt                                                                                      <  2:1247723/100‑1 (MQ=255)
                 tACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttagtg                                                                                   <  1:1091939/100‑5 (MQ=38)
                  atcGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAAGTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttagtgc                                                                                  <  1:2092610/98‑6 (MQ=38)
                  aCCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCAc                                                                                  <  2:1456909/100‑1 (MQ=255)
                   ccGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACg                                                                                 <  2:67504/100‑1 (MQ=255)
                   ccGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACg                                                                                 <  2:1626822/100‑1 (MQ=255)
                     gTTGGCGCGGGTGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttagtgcgca                                                                               <  1:2729166/100‑9 (MQ=37)
                     gTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCt                                                                               <  1:2374017/100‑1 (MQ=255)
                        ggCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTc                                                                            <  1:2032622/100‑1 (MQ=255)
                          cgcgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCttt                                                                             >  2:1772705/1‑97 (MQ=255)
                          cgcgGGCGGTGTAGCAAAAGTTCTGAGCTAATTGCGGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGt                                                                          >  2:2121008/1‑100 (MQ=255)
                           gcgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGt                                                                          >  2:1063779/1‑99 (MQ=255)
                            cgGGCGTTGTAGCAAAAGTTATGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGAGCATCATTTGATGCCATTTTTGCACGCTTTCGTAc                                                                        <  2:2538894/100‑1 (MQ=255)
                               gCGTTGTAGCAAAAGTTCTGAGATAATTGCCGATAACATTTGACGCAATGCGAACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGt                                                                          <  2:216032/95‑1 (MQ=255)
                                   tGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCTCTTTTTGCACGCTTTCGTACCAGAAcc                                                                 <  2:113996/100‑1 (MQ=255)
                                   tGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAAcc                                                                 <  1:652061/100‑1 (MQ=255)
                                      aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTgg                                                              >  1:2681470/1‑100 (MQ=255)
                                      aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTgg                                                              >  2:2961892/1‑100 (MQ=255)
                                      aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTgg                                                              >  2:2397126/1‑100 (MQ=255)
                                      aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATAATTTGATACCCTTTTTGCACGCTTTCGTACCAGAACCTg                                                               >  1:1081649/1‑99 (MQ=255)
                                       tcttccgatCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             <  2:2546236/92‑1 (MQ=255)
                                       gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             <  1:1453245/100‑1 (MQ=255)
                                       gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             <  1:1881887/100‑1 (MQ=255)
                                       gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             <  2:866223/100‑1 (MQ=255)
                                       gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             >  2:2210291/1‑100 (MQ=255)
                                       gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGAGCACTAAAAGTGAATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAAcc                                                                 >  2:871956/1‑96 (MQ=255)
                                        cAAAAGTTCTGAGCTAATTGCCGATAACATTTGTAGCAATGCGCACTAAAAAGGCATCATTTGATGCCCTTTTTGCACGCCTTCGTACCAGAACCTCGCt                                                            >  2:1977468/1‑100 (MQ=255)
                                        cAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCt                                                            >  2:1602056/1‑100 (MQ=255)
                                        cAAAAGTTCTGAGCTAATTGCAGATAACATTTGACGCAATGCGCGCTAAAAGGGCATCATTTGATGCTCATGTTGCACGCTGTCGTACGAGAACCTgg                                                              >  2:2639686/1‑98 (MQ=255)
                                            tccgatctgCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcatc                                                        <  2:1170604/92‑1 (MQ=255)
                                             gTTCTGATCTAATTGCCGATAACATTTGACGCAATGCGCACTGAAAGCGCATCATTTGATGCTCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcagc                                                        <  2:775547/99‑3 (MQ=255)
                                              ttCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAg                                                      <  2:1629446/100‑1 (MQ=255)
                                                cTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTg                                                    >  2:2074728/1‑100 (MQ=255)
                                                    gCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAgatcgga                                                >  1:1170615/1‑94 (MQ=255)
                                                    gCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAttt                                                <  2:1024789/100‑1 (MQ=255)
                                                     cTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAtttt                                               >  1:2325112/1‑100 (MQ=255)
                                                         ttGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCttt                                           <  1:2357043/100‑1 (MQ=255)
                                                          tGCTGATATCATTTGACGTAATGCGCACTAAAAGTGTATCATTTGATGTTCTTTTTGTACGCTTTCGTACCAGAACTTGGCTCATCAGTGATTTTCTTTg                                          <  2:1627486/100‑1 (MQ=255)
                                                          tGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                          >  1:982556/1‑100 (MQ=255)
                                                           gCCGATAACATTTGACGCAATGCGCACTAAAAGTGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTTTCTCATCAGTGATTATCTTTg                                          >  1:1329641/1‑99 (MQ=255)
                                                           gCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGt                                         >  2:1245876/1‑100 (MQ=255)
                                                           gCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGt                                         >  2:1941426/1‑100 (MQ=255)
                                                            ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTc                                        <  2:2325086/100‑1 (MQ=255)
                                                            ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTc                                        >  2:1997725/1‑100 (MQ=255)
                                                             cGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCa                                       <  2:2647986/100‑1 (MQ=255)
                                                             cGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGATCATCAGTGATTTTCTTTGTCa                                       >  2:829334/1‑100 (MQ=255)
                                                                tAACATTTGACGTAATGCGCACTAAAAAGACATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAATCTAGCTCATCAGTGATTTTCTTTGTCATa                                     >  2:748549/1‑99 (MQ=255)
                                                                 aaCATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAACAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAAt                                   >  2:1743461/1‑100 (MQ=255)
                                                                  aCATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATc                                  >  1:2292884/1‑100 (MQ=255)
                                                                     ttggACGCAATGCTCACTAACAGGGCATCAGTTGATGCTCTTTTTTCATGTTTTCGGACCAGAACCTGGTTCATCAGTGATTTTCTTTGTCATAATCAtt                               <  2:1010760/97‑1 (MQ=255)
                                                                        gACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCt                            >  1:3028223/1‑100 (MQ=255)
                                                                         aCGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGTCTCATCAGTGATTTTCTTTGTCATAATCATTGCTg                           <  2:2996949/100‑1 (MQ=255)
                                                                          cGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCAAGCTTTCGTACCAGAAACTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTga                          >  1:1530011/1‑100 (MQ=255)
                                                                          agCAATGCGCACTAAAAAAACATCATTTGATGCCATTTTTGCACGCTTTCGTACCAGAACCTGGCTAATCAGTGATTTTCTTTGTCATAATCATTGCTga                          >  2:1296120/2‑100 (MQ=255)
                                                                           gttattctcgcTAAAAGGTTATCATTTGATGCCCTGTTTTCACGCTTGCGTACCAGACCCTTTTTCATCAGTGATTTTCTTTGTCATAATCAGTGCTgag                         <  2:1803328/90‑1 (MQ=255)
                                                                           gCAATGCGCACTAAAAGGGTATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTga                          <  1:1745732/99‑1 (MQ=255)
                                                                           gCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTgag                         <  2:2823026/100‑1 (MQ=255)
                                                                             aaagcgtgcaaaaaGGGCATCAAATGATGCCCTTTTAGTGCGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGAc                       >  2:50479/11‑100 (MQ=18)
                                                                               tGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAg                     >  2:371031/1‑100 (MQ=255)
                                                                               tGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAg                     >  1:138641/1‑100 (MQ=255)
                                                                                gcgcACTAAAAGGGCATCATTTGATGTCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAgg                    <  1:747654/100‑1 (MQ=255)
                                                                                 ctcACTAATAGGGTATCATTTGATGCCCTTTTTGTTCGTTTTCGTACTATAACCTGTGTCATAAGTGATTGTCTTTTTCATAATCATTGCTga                          <  2:2867413/91‑1 (MQ=255)
                                                                                 cgtgcacacaGGGCATCAAATGATGACCTTTTTGCACGCTTTCGTACCAGAACCAGGCTCAAAAGTGATTCTCTTTGTCATAATCATTGCTga                          >  2:14289/10‑93 (MQ=255)
                                                                                  gcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAgg                    <  2:3044939/98‑1 (MQ=255)
                                                                                  gcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGc                   >  1:1200032/1‑99 (MQ=255)
                                                                                    aCTAAAAGGGCATCATTGTATGCCCTTTTTGTACGCTTTCGTACCAGAACCTGGCGCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctc                 <  2:2442296/99‑1 (MQ=255)
                                                                                     caaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAAGctct                >  1:2242441/3‑99 (MQ=38)
                                                                                     cTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTg               >  1:2656400/1‑100 (MQ=255)
                                                                                      aaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGt              >  2:1808330/2‑100 (MQ=255)
                                                                                       aaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGtt             >  2:1245886/1‑100 (MQ=255)
                                                                                        aaaGGGCATCATTTGATGCCCTTTTTTTTTTTTTTTGTACCAGAACCTGGCTCATCAGTGTTTTTCTTTGTCATAATCATTGCTTAGACAGGCTCTGtt             >  2:930223/1‑99 (MQ=255)
                                                                                        aaaGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTg            <  2:646425/100‑1 (MQ=255)
                                                                                         aaGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGa           <  1:1263939/100‑1 (MQ=255)
                                                                                         aaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGa           >  1:1246750/1‑100 (MQ=255)
                                                                                         aaGGGCATCAAATGATGCCCTTTTTGAACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGa           >  2:705957/1‑100 (MQ=255)
                                                                                          aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAg          <  1:2479866/100‑1 (MQ=255)
                                                                                               atcatTTGATGCCCTTTTTGCACGCTTTCTTATCAGAACCTGGTTCATCAGTAATTTTCTTTGTGATTATCATTGCTTAGATATGCTGTGTTGAGGGCg      <  2:2451468/99‑1 (MQ=255)
                                                                                                tcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGta    <  1:2599098/100‑1 (MQ=255)
                                                                                                 catTTGATGCCCTTTTTGCACGCTTTCGTACCAGTTCCTGGCTCATCAGTGATTTTCTTTGTCATAATCATGGCTGAGACAGGCTCTGTTGAGGGCGtat   <  1:192186/100‑1 (MQ=255)
                                                                                                 catTTGATGCCCTTTTTGCACGCTTTCGTACCAGATCCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGtat   <  1:1772725/100‑1 (MQ=255)
                                                                                                  atTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGtata  >  2:189089/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
ATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATA  >  NC_000913/4177072‑4177269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: