Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,279,370 G→T 100% A298S (GCA→TCA)  ghxP → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,3700GT96.4% 355.4 / 4.1 110A298S (GCA→TCA) ghxPguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (52/54);  total (54/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 110 total aligned reads displayed.

GATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGG  >  NC_000913/4279273‑4279466
                                                                                                 |                                                                                                
gatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATca                                                                                                <  1:963063/100‑1 (MQ=255)
 atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                               <  2:2877084/100‑1 (MQ=255)
  tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcact                                                                                              >  2:899950/1‑100 (MQ=255)
  tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcact                                                                                              >  2:2544572/1‑100 (MQ=255)
  tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcact                                                                                              >  1:2918431/1‑100 (MQ=255)
   gaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactg                                                                                             <  2:2513439/100‑1 (MQ=255)
   gaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactg                                                                                             <  2:475360/100‑1 (MQ=255)
     cGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgac                                                                                           <  1:278458/100‑1 (MQ=255)
     cGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgac                                                                                           <  1:567456/100‑1 (MQ=255)
      ggCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgacc                                                                                          <  2:793163/100‑1 (MQ=255)
       gCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgacca                                                                                         >  2:2015624/1‑100 (MQ=255)
         aGTTTTTGATGTTATCTGAACTATCCGTGTTGTGGTCGGTCAGGCGAACCTGCTGGATAAAGATGGGCAGATCAACGACGGTGGGAAATcactgaccact                                                                                       <  1:575564/100‑1 (MQ=255)
         aGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccact                                                                                       <  2:1478034/100‑1 (MQ=255)
         aGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccact                                                                                       <  1:2125838/100‑1 (MQ=255)
            aTTTGATGCCACCGGCACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccactgac                                                                                    <  1:2995074/100‑1 (MQ=255)
            aTTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccactgac                                                                                    >  1:710275/1‑100 (MQ=255)
            aTTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccactgac                                                                                    >  2:2089381/1‑100 (MQ=255)
            aTTTGATGCCACCGGAACTATCACTGCAGCCGCCGGCCAGGAGAACCTCCTGCATAAAGATGCGCAGATCATCCACGGGGGAAAATcactgagcactggc                                                                                    >  2:2416389/1‑98 (MQ=255)
             tttGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATAGACGGTGGGAAATCACTGACCACTGACt                                                                                   >  1:1414711/1‑100 (MQ=255)
               tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTcc                                                                                 >  1:2920573/1‑100 (MQ=255)
                gATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCa                                                                                <  1:1517781/100‑1 (MQ=255)
                gATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCa                                                                                <  1:1517246/100‑1 (MQ=255)
                  tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                              <  1:198726/100‑1 (MQ=255)
                  tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGACAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCAt                                                                               >  1:1694666/1‑99 (MQ=255)
                   gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGa                                                                             >  2:2324750/1‑100 (MQ=255)
                    ccACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGag                                                                            <  1:2382370/100‑1 (MQ=255)
                    ccACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTAACTCCATGa                                                                             >  1:453114/1‑99 (MQ=255)
                     cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagc                                                                           >  1:1983976/1‑100 (MQ=255)
                      aCCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATTGGCAGATCATCGACGGTGGGAAATCTCTGACCACTGACTCCATGagc                                                                           <  2:2903411/99‑1 (MQ=255)
                      aCCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGTAAATCACTGACCACTGACTCCATGagca                                                                          <  2:1276625/100‑1 (MQ=255)
                      aCCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagca                                                                          <  2:1350013/100‑1 (MQ=255)
                       ccGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagc                                                                           <  2:1898558/98‑1 (MQ=255)
                         ggAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCg                                                                       >  1:21690/1‑100 (MQ=255)
                          gAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGt                                                                      >  1:803840/1‑100 (MQ=255)
                          gAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGt                                                                      >  2:1411034/1‑100 (MQ=255)
                             cTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGtttt                                                                   >  2:2549663/1‑100 (MQ=255)
                             cTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGtttt                                                                   <  1:648993/100‑1 (MQ=255)
                                tCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGCTAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCTGCGTTTtctc                                                                >  2:1999372/1‑100 (MQ=255)
                                tCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGTAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtctc                                                                <  1:2486610/100‑1 (MQ=255)
                                tCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtctc                                                                <  2:2920541/100‑1 (MQ=255)
                                tCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtctc                                                                >  1:1753558/1‑100 (MQ=255)
                                 ccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCCGCGTTTTctct                                                               >  2:2728164/1‑100 (MQ=255)
                                 ccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTctct                                                               >  2:1915855/1‑100 (MQ=255)
                                 ccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTctct                                                               >  2:2328767/1‑100 (MQ=255)
                                   gTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTgg                                                             <  2:53972/100‑1 (MQ=255)
                                   gTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                                                              <  1:1355056/99‑1 (MQ=255)
                                    tGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                                                            <  1:1930199/100‑1 (MQ=255)
                                     gCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGcc                                                           >  1:1254528/1‑100 (MQ=255)
                                       cGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtg                                                         <  1:1239312/100‑1 (MQ=255)
                                       cGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtg                                                         >  2:2372163/1‑100 (MQ=255)
                                        gTCGCCGGCCAGGCGAATCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtgg                                                        <  1:708946/100‑1 (MQ=255)
                                          cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtg                                                      <  1:997597/100‑1 (MQ=255)
                                           gCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtgg                                                     >  1:1883615/1‑100 (MQ=255)
                                              ggCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg                                                  >  2:1683436/1‑100 (MQ=255)
                                                   ggCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCt                                             >  2:2105200/1‑100 (MQ=255)
                                                   ggCGAACATGATGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTAACTCCATGAGCAGCGCTTTCTCTGGCCTGGTGAGTGc                                                 >  2:555658/1‑96 (MQ=255)
                                                      gAACCTGCTGGATAAAGATGGGTAGTTTATCGTTTGTGGGAAATCATTGACCAATGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCg                                          <  1:290713/100‑1 (MQ=255)
                                                       aaCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCgg                                         <  1:1404327/100‑1 (MQ=255)
                                                           tgctgGATAAAGATGGGCAGATCATCGATGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGc                                     <  1:1824775/100‑1 (MQ=255)
                                                            gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCg                                    >  2:2020771/1‑100 (MQ=255)
                                                            gctgAATAAAGATGGGCAGATCATCGAAGGAGGGAAATGACTGACCACTGACTCCATGAGCAGTGTGGTTTCTTGCCGGGTGGGTGCGGCTcctg                                         >  2:247001/1‑93 (MQ=255)
                                                             ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                   <  2:858888/100‑1 (MQ=255)
                                                             ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                   <  1:1425718/100‑1 (MQ=255)
                                                             ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                   <  1:924531/100‑1 (MQ=255)
                                                              tgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGt                                  >  2:2034551/1‑100 (MQ=255)
                                                               ggATAAAGATGGGCATATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                 >  2:443277/1‑100 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                 <  1:1178768/100‑1 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                 >  1:2006711/1‑100 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                 <  1:2411344/100‑1 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                 <  1:2915643/100‑1 (MQ=255)
                                                                gATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                 <  1:2034574/99‑1 (MQ=255)
                                                                 aTAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtata                               <  1:1457945/100‑1 (MQ=255)
                                                                  tAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTATCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATAc                              >  1:700865/1‑100 (MQ=255)
                                                                   aaaGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACa                             >  2:208076/1‑100 (MQ=255)
                                                                    aaGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGGGGTATACa                             >  2:2763070/1‑99 (MQ=255)
                                                                     aGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATc                           <  2:848244/100‑1 (MQ=255)
                                                                     aGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATc                           <  1:2151961/100‑1 (MQ=255)
                                                                     aGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGAGGGTGCGGCTCCGGCAGCGGTATACATc                           >  1:616309/1‑100 (MQ=255)
                                                                        tGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAg                        <  2:2246528/100‑1 (MQ=255)
                                                                        tGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAg                        >  2:795009/1‑100 (MQ=255)
                                                                        tGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAg                        <  1:2737755/100‑1 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                      >  2:2704840/1‑100 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                      <  2:757748/100‑1 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                      >  2:1366082/1‑100 (MQ=255)
                                                                           gCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCt                     >  1:1908712/1‑100 (MQ=255)
                                                                           gCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGCTGCGGCTCCGGCAGCGGTATACATCGAGTCt                     >  2:2377551/1‑100 (MQ=255)
                                                                            cAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCtn                    <  2:27783/100‑2 (MQ=255)
                                                                            cAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTg                    >  1:2284880/1‑100 (MQ=255)
                                                                              gATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcg                  >  2:2616035/1‑100 (MQ=255)
                                                                               atcatcGACGGTTGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcgg                 <  1:2082833/100‑1 (MQ=255)
                                                                               atcatcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcgg                 <  1:378120/100‑1 (MQ=255)
                                                                                 catcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg               <  1:292713/100‑1 (MQ=255)
                                                                                   tcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCggg             <  2:2316279/100‑1 (MQ=255)
                                                                                    cGACGGTGGGAAATCACTGACCACTGGCGCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGt            <  2:2008410/100‑1 (MQ=255)
                                                                                      aCGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGACTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTa           >  2:2376859/1‑99 (MQ=255)
                                                                                       cGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcg         <  1:2396231/100‑1 (MQ=255)
                                                                                         gTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcgg        >  2:1312406/1‑99 (MQ=255)
                                                                                          tGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTACGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcg      >  1:2403969/1‑100 (MQ=255)
                                                                                             gAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcg   <  1:2581282/100‑1 (MQ=255)
                                                                                              aaaTCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcgg  >  1:2801723/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGG  >  NC_000913/4279273‑4279466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: