Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,060 C→T 32.6% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT32.6% 340.3 / 219.2 269intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (104/77);  new base T (40/48);  total (144/125)
Fisher's exact test for biased strand distribution p-value = 6.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-02

Only 100 of 272 total aligned reads displayed.

CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG  >  NC_000913/4295961‑4296159
                                                                                                   |                                                                                                   
 ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg                                                                                                    <  1:1660209/100‑1 (MQ=33)
  tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                                                                                   >  1:1909528/1‑100 (MQ=18)
   aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc                                                                                                  <  1:258325/100‑1 (MQ=18)
   aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc                                                                                                  <  1:806242/100‑1 (MQ=18)
   aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc                                                                                                  >  1:1343509/1‑100 (MQ=18)
   aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc                                                                                                  >  2:2230610/1‑100 (MQ=18)
    ccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                  <  2:2965913/98‑1 (MQ=17)
    aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGccn                                                                                                 <  2:645309/100‑2 (MQ=18)
     cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTg                                                                                                >  1:2330928/1‑100 (MQ=17)
     cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                >  1:1781682/1‑100 (MQ=32)
      gtgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                               <  1:38073/98‑1 (MQ=16)
      gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTTTATAATGGATAAGGCGTTTACGCCGCATCAGACATCAATGCCTGa                                                                                               >  2:946492/1‑100 (MQ=11)
      gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                               >  1:652772/1‑100 (MQ=33)
      gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                               <  1:2091813/100‑1 (MQ=33)
       cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                              <  2:2748068/100‑1 (MQ=18)
       cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                              >  2:2111681/1‑100 (MQ=33)
       cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                              >  2:2616269/1‑100 (MQ=32)
        gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg                                                                                             >  2:2398826/1‑100 (MQ=18)
         tccgatctggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                            <  2:183691/92‑1 (MQ=255)
           ttATCAGGCCTACGCCAGACAGGGCAATAGCCTGATTTAGCTTGATTTTGTAGATCGGATAAGGCGTTTACGCGGCATCCGACATCAACGCCTGATGCg                                                                                           >  2:1503324/1‑99 (MQ=11)
            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                           >  1:1121888/1‑98 (MQ=17)
            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                         <  1:473181/100‑1 (MQ=18)
            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCgcc                                                                                         <  1:2061341/100‑3 (MQ=17)
             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg                                                                                        <  1:620715/100‑1 (MQ=18)
             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                        >  1:2337375/1‑100 (MQ=32)
              tCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGc                                                                                       <  1:57576/100‑1 (MQ=18)
              tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                       <  2:2330901/100‑1 (MQ=17)
               catcccTCCGCCAGTCTTCGCATTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt                                                                                      <  1:863031/96‑1 (MQ=11)
               cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                      >  2:1434093/1‑100 (MQ=32)
               cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt                                                                                      >  2:1999543/1‑100 (MQ=32)
                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                    >  2:2142371/1‑100 (MQ=25)
                 ggCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGCCGTTTACTCCGCATCCTACATCAACACCAGAGGCGTCGCtg                                                                                     >  2:2846530/1‑98 (MQ=11)
                    cTACGCCAGCCAGCGCAATAGCCTGATTTAGTGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg                                                                                 >  2:855261/1‑100 (MQ=255)
                     tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCCCATCCGACATCAACCCCTCATCCGACGCTTccc                                                                                  >  2:2601613/1‑98 (MQ=11)
                      aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg                                                                               <  2:2932873/100‑1 (MQ=40)
                       cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                              >  1:2977930/1‑100 (MQ=38)
                       cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAGGCGACGCTTGCcgcg                                                                               >  1:1964794/1‑99 (MQ=255)
                         ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                            <  2:1825503/100‑1 (MQ=25)
                         ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATACGACGCTTGACGCGTCt                                                                            >  1:718898/1‑100 (MQ=16)
                         ccAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTGGGGTAAGGCGGTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg                                                                               <  2:124638/97‑1 (MQ=11)
                             aCAGCGCAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATC                                                                        <  1:1241436‑M1/100‑1 (MQ=255)
                                gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGG                                                                     >  1:47525‑M1/1‑100 (MQ=255)
                                gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGG                                                                     >  2:1200059‑M1/1‑100 (MQ=255)
                                 cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGC                                                                    <  1:938868‑M1/100‑1 (MQ=255)
                                 cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGC                                                                    >  2:1114376‑M1/1‑100 (MQ=255)
                                  gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                   <  1:2848801/100‑1 (MQ=25)
                                   cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                   <  2:2675440/99‑1 (MQ=38)
                                   cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                  >  2:605768/1‑100 (MQ=40)
                                   cAATAGCCTAATTTAGCGTGATTTTGTAGGTAGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                   <  1:2007636/99‑1 (MQ=255)
                                    aaTAGCCTGATTTAGCGTGATTTTGTAGGTCTGATAAGGCGTTTACGCCGCATCCGATATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                 <  2:1375361/100‑1 (MQ=255)
                                    aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                 <  1:689454/100‑1 (MQ=255)
                                    aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                 <  2:2264673/100‑1 (MQ=255)
                                     aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                >  1:1825524/1‑100 (MQ=255)
                                      tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                               <  2:1343497/100‑3 (MQ=16)
                                      tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg                                                               <  1:2587171/100‑1 (MQ=255)
                                       aGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGTCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                              <  1:542310/100‑4 (MQ=11)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                             >  2:1897482/1‑96 (MQ=9)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                             >  2:753096/1‑96 (MQ=9)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATACGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                             >  1:2527077/1‑96 (MQ=255)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                             >  2:596250/1‑100 (MQ=255)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                             >  1:1580975/1‑100 (MQ=40)
                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                             <  1:74356/100‑1 (MQ=255)
                                         ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                            >  1:871084/1‑95 (MQ=2)
                                         ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                            <  1:1268513/100‑6 (MQ=2)
                                         ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                            >  1:2465891/1‑100 (MQ=40)
                                            gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGACCTATCCCAGAc                                                         >  2:2046483/1‑100 (MQ=14)
                                             aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAc                                                         <  1:2669502/99‑1 (MQ=25)
                                               ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCTGGCgttggcctgataag                                                      <  2:1583714‑M1/100‑15 (MQ=255)
                                                tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg                                                     <  2:781144/100‑1 (MQ=255)
                                                 aGCGTGATTTTGTAGGTCGTATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc                                                    <  2:2511292/100‑1 (MQ=255)
                                                 aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACTCCGCATCCTACAGCAACGGCTGATGCGACGCTTGCCGTTTCTTATTAGGCCTACGCCAGACAgcg                                                     <  2:671725/99‑1 (MQ=14)
                                                     tGATTTTGTAGGTCGGATAAGGCGTTTTCGCCGCATCCGACATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc                                                    <  2:2489849/96‑1 (MQ=255)
                                                           tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                           >  2:504652/1‑99 (MQ=18)
                                                            tgaGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                         >  2:344862/3‑100 (MQ=255)
                                                              atgTCGGATGCGGCGTAAACGCCTTATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAttt                                       >  1:1626365/3‑100 (MQ=11)
                                                               ggTCGGATAAGGCGTTTACGCCTCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                      <  1:2411450/100‑1 (MQ=255)
                                                               ggTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                      >  2:167033/1‑100 (MQ=255)
                                                                gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                     >  2:2171016/1‑100 (MQ=255)
                                                                 tCGGATGCGGCGTAAACGCCTTATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCATGATTTAg                                     >  1:337819/1‑99 (MQ=14)
                                                                      tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCTCGAGACAGCGCCGTAGAGTGATTGAGCGTgct                               >  2:2007613/1‑98 (MQ=11)
                                                                         ggCGTTTACGCCGCATCCGACATCACCGCCTGATGCGACGCTTGGCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                            <  2:2098736/100‑1 (MQ=255)
                                                                             tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                        <  1:596254/100‑1 (MQ=40)
                                                                                 cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG                    >  1:1678094‑M1/1‑100 (MQ=255)
                                                                                  gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGG                   <  2:118249‑M1/100‑1 (MQ=255)
                                                                                   ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGA                  <  1:2496629‑M1/100‑1 (MQ=255)
                                                                                   ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAACCTGATTTAGCGTGATTTTGTAGGTCGGA                  >  1:146516‑M1/1‑100 (MQ=255)
                                                                                    cgcATCCGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG                    <  2:2794321‑M1/97‑1 (MQ=255)
                                                                                    cgcATACGGTATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGn                  <  2:2906892‑M1/99‑2 (MQ=255)
                                                                                       aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg              >  1:129503/1‑100 (MQ=38)
                                                                                         ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGc            >  1:2911472/1‑100 (MQ=38)
                                                                                             cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt        <  2:47288/100‑1 (MQ=35)
                                                                                              aTCAACGTCTAATGCGATGCTTGTCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa       <  1:1048131/100‑1 (MQ=11)
                                                                                              aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt         <  1:1101676/98‑1 (MQ=35)
                                                                                                 aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc    >  1:2234569/1‑100 (MQ=32)
                                                                                                  aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCgttgatgtcggatgcggcgtaaacgccttatccgacctacaaaatcacgctaaatcaggctattg   >  2:2436858‑M1/1‑35 (MQ=255)
                                                                                                  aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc   <  2:1964771/100‑1 (MQ=31)
                                                                                                  aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc   >  2:542307/1‑100 (MQ=31)
                                                                                                  aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc   >  1:2779898/1‑100 (MQ=31)
                                                                                                  aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTTATTTACCGTGATTTTGTATGTCGGATAAGGCGgttctacc   >  2:1354/1‑95 (MQ=11)
                                                                                                  aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCgttgatgtcggatgcggcataaacgccttatccgacctacaaaatcacgctaaatcaggctattg   >  2:433954‑M1/1‑35 (MQ=255)
                                                                                                   |                                                                                                   
CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCG  >  NC_000913/4295961‑4296159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: