BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,340,287	G→A	100%	L79F (CTT→TTT)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,340,287	0	G	A	99.2%	415.7 / ‑4.8	130	L79F (CTT→TTT)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (60/68); total (60/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

Only 100 of 130 total aligned reads displayed.

TCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAA  >  NC_000913/4340190‑4340382

 cATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACtt                                                                                              <  1:2591733/100‑1 (MQ=255)

 cATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACtt                                                                                              >  1:1605732/1‑100 (MQ=255)

  aTCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTa                                                                                             <  2:673783/100‑1 (MQ=255)

   tCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACCGGAAGACCGGCACGTTTTGTTGGCGATCATGAAACTTAc                                                                                            >  1:1878171/1‑100 (MQ=255)

   tCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAc                                                                                            >  1:3582/1‑100 (MQ=255)

     gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                          <  2:541832/100‑1 (MQ=255)

     gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                          >  2:2889676/1‑100 (MQ=255)

      aCAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAACGa                                                                                         >  2:2898278/1‑100 (MQ=255)

       cAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAAGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGAt                                                                                        >  1:2428702/1‑100 (MQ=255)

        aaGCTCCAGCAGGTCGCGATCCATTGCTGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATc                                                                                       <  1:529289/100‑1 (MQ=255)

        aaGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATc                                                                                       <  1:1063284/100‑1 (MQ=255)

        aaGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATc                                                                                       >  2:848763/1‑100 (MQ=255)

         aGCTCCAGCAGGTCGCGATACATTGCGGCGAGGGCTTTTTCCCGATAGCCCAACAGAGAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCa                                                                                      >  2:365832/1‑100 (MQ=255)

           cTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCTAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAAt                                                                                    <  1:26576/100‑1 (MQ=255)

            tCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAAtt                                                                                   >  1:2516497/1‑100 (MQ=255)

             ccAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTg                                                                                  <  1:1424517/100‑1 (MQ=255)

             ccAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTg                                                                                  <  2:1117986/100‑1 (MQ=255)

               agcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTc                                                                                >  2:2473667/1‑100 (MQ=255)

               agcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTc                                                                                >  2:11339/1‑100 (MQ=255)

                  agGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGa                                                                             <  2:2013427/100‑1 (MQ=255)

                   ggTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGTTCGCCCAACAAGAAGACCGGCACGTTTTGTTGGAGCTCAAGAAACTTACCGATAAATTGTTTGAc                                                                            >  1:2036347/1‑100 (MQ=255)

                   ggTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGa                                                                             >  2:1556125/1‑99 (MQ=255)

                   ggTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGATTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGAc                                                                            >  1:1049738/1‑100 (MQ=255)

                     tCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGt                                                                          <  2:698542/100‑1 (MQ=255)

                        cgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGtttt                                                                       <  2:2790893/100‑1 (MQ=255)

                        cgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGtttt                                                                       >  1:2348925/1‑100 (MQ=255)

                        cgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGtttt                                                                       <  1:1101354/100‑1 (MQ=255)

                         gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTg                                                                      <  2:277109/100‑1 (MQ=255)

                         gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTg                                                                      >  1:2684404/1‑100 (MQ=255)

                         gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTg                                                                      <  1:1926542/100‑1 (MQ=255)

                           tCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGAt                                                                    <  2:1010262/100‑1 (MQ=255)

                            ccATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATg                                                                   <  2:1794040/100‑1 (MQ=255)

                            ccATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATg                                                                   <  1:1315061/100‑1 (MQ=255)

                             cctttcggcgAGGGCTTTTTAGCGATCGACCAACAAGAAGACCGGTACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGt                                                                  >  2:1323031/6‑100 (MQ=255)

                             cATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGt                                                                  <  2:1298516/100‑1 (MQ=255)

                              aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                 <  2:1605713/100‑1 (MQ=255)

                              aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGATTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                 >  2:1722276/1‑100 (MQ=255)

                                tGCGGCTAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                               <  1:2815651/100‑1 (MQ=255)

                                tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTcn                                                               <  2:2788418/100‑2 (MQ=255)

                                 gcggcgAGGGCTTTTTCCCGATAGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGt                                                              >  2:1758993/1‑100 (MQ=255)

                                   ggcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTcc                                                            >  1:2811350/1‑100 (MQ=255)

                                    gcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCg                                                           >  1:2009117/1‑100 (MQ=255)

                                     cgAGGGCTTTTTCCTGATCTCCCAAAAAGAAGACCAGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTCTCTGCCGTTTTAATGTTCGTcag                                                           >  2:1914746/1‑97 (MQ=255)

                                      gAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGa                                                         <  2:2811318/100‑1 (MQ=255)

                                       aGGGCTTTTTCCCGATCGCCCAACAGGAAGATTTGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGAt                                                        <  1:813660/100‑1 (MQ=255)

                                        gggCTTTTTCCTTATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGAt                                                        >  2:2341862/1‑99 (MQ=255)

                                        gggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATg                                                       >  2:1707738/1‑100 (MQ=255)

                                        gggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATg                                                       >  1:304901/1‑100 (MQ=255)

                                           cTTTTTCGCGATCGCCCAACAGGAAGAACAGTACGTTTTGTTGGGGCACATGAAAATTAGCAGTAAATTGTATCACGTTTTGATGTTCGTCCGGATg                                                       >  2:710828/1‑97 (MQ=255)

                                           cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                    >  1:2796730/1‑100 (MQ=255)

                                              tttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAt                                                 <  1:2975371/100‑1 (MQ=255)

                                               ttCCCGATCGCCCAACAGGAAGACCGGTACGTTTTGTTGTCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                                <  1:918964/100‑1 (MQ=255)

                                               ttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                                >  1:64808/1‑100 (MQ=255)

                                               ttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                                <  2:2728332/100‑1 (MQ=255)

                                                tCCCGATCGCCCAACAGTAAGACCGGCACGTTTTGTTGGCCCTCATGAAACTTACCTATCAATTGTCTGTCGTTTTGATGTTCGTCCGGATGTTCCAttt                                               <  2:2630169/100‑1 (MQ=255)

                                                tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                               >  1:1660608/1‑100 (MQ=255)

                                                tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTCTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                               >  2:1842820/1‑100 (MQ=255)

                                                 cccGTTCGCCCAATAGGAAGATCGGCACGTTTTGTTGGTGCTCATGAAACTTACAGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTg                                              <  1:755409/100‑1 (MQ=255)

                                                 cccGCTCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                               <  2:2873357/99‑1 (MQ=255)

                                                  ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                             >  1:2312824/1‑100 (MQ=255)

                                                  ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                             >  1:322865/1‑100 (MQ=255)

                                                  ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATAAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                             >  1:323095/1‑100 (MQ=255)

                                                      tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGTGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGc                                         <  1:2886850/100‑1 (MQ=255)

                                                       cGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCt                                        >  2:2529097/1‑100 (MQ=255)

                                                       cGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCt                                        <  1:2194579/100‑1 (MQ=255)

                                                        gCCCAACAGGAAGACCGGCACGTTTTGTTGTCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                       <  2:2266137/100‑1 (MQ=255)

                                                        gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                       <  2:533537/100‑1 (MQ=255)

                                                        gCCCAACAGGAAGACAGGCACGTTTTGTTGGAGCACATGAAACTTACCGATCAATTGTATGACGGTTTGATGTTCGTCCGAATGTTCAATTTGACAg                                          >  2:2503566/1‑97 (MQ=255)

                                                         cccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGa                                      <  2:187632/100‑1 (MQ=255)

                                                          ccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACATTTTGATAATCGTCCGGATGTTCCATTTGATAACTGaa                                     >  1:1636463/1‑100 (MQ=255)

                                                            aatatgAATACCTGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACa                                   <  1:679242/95‑1 (MQ=255)

                                                             aCAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTACGTCCGGATGTTCCATTCGATAGCTGAACAt                                  <  1:2041424/100‑1 (MQ=255)

                                                                ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                               >  1:2311412/1‑100 (MQ=255)

                                                                ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                               <  1:1026275/100‑1 (MQ=255)

                                                                 gAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAgg                              <  2:541582/100‑1 (MQ=255)

                                                                  aaGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                             <  1:2879068/100‑1 (MQ=255)

                                                                  aaGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGc                             <  1:464641/100‑1 (MQ=255)

                                                                    gACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAg                           <  1:185841/100‑1 (MQ=255)

                                                                      ccGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTc                         <  2:827590/100‑1 (MQ=255)

                                                                       cGGCACGTTTTGTTGGCGCTCATGAATCTTGCCGATCAATTTTCTGACGTTTTGATTTTCGTCCGGATGTTTCATTTGATAGCTGAACATCAGGCAGt                          <  2:1258064/98‑1 (MQ=255)

                                                                       cGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATTAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCa                        <  1:1663685/100‑1 (MQ=255)

                                                                        ggCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAATATCAGGCAGTCaa                       >  2:331500/1‑100 (MQ=255)

                                                                        ggCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTc                         <  2:808477/98‑1 (MQ=255)

                                                                            cGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATgn                    <  2:2591845/99‑2 (MQ=255)

                                                                            cGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGc                   <  2:1980758/100‑1 (MQ=255)

                                                                              ttttGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGGAGTCAATGGCtt                 <  2:948240/100‑1 (MQ=255)

                                                                                ttgttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGGTAGCTGAACATCAGGCAGTCAATGGCTTCg               <  1:1414615/100‑1 (MQ=255)

                                                                                  gttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt             <  2:872844/100‑1 (MQ=255)

                                                                                    tgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTg           <  2:2959897/100‑1 (MQ=255)

                                                                                    tgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTg           >  2:1545345/1‑100 (MQ=255)

                                                                                       cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag        >  2:289609/1‑100 (MQ=255)

                                                                                        gcTCATTAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag        <  1:2269727/99‑1 (MQ=255)

                                                                                         cTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagag      >  2:634596/1‑100 (MQ=255)

                                                                                           cATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag    <  1:1474843/100‑1 (MQ=255)

                                                                                           cATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag    <  2:1328769/100‑1 (MQ=255)

                                                                                           cATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag    <  2:1674738/100‑1 (MQ=255)

                                                                                           cATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag    >  2:2326200/1‑100 (MQ=255)

                                                                                            aTGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag    <  1:1477651/99‑1 (MQ=255)

                                                                                            aTGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga   >  2:649040/1‑100 (MQ=255)

                                                                                            aTGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga   >  1:1386822/1‑100 (MQ=255)

TCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAA  >  NC_000913/4340190‑4340382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`