Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,360,814	T→G	100%	Y374S (TAC→TCC)	cadC ←	cadBA operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,360,814	0	T	G	100.0%	322.5 / ‑6.7	97	Y374S (TAC→TCC)	cadC	cadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (41/55);  total (42/55)

TTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCCT  >  NC_000913/4360718‑4360910                                                                                                 |                                                                                                 ttATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAgg                                                                                               <  1:1095044/100‑1 (MQ=255) ttATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAgg                                                                                               <  1:3036759/100‑1 (MQ=255) ttATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAgg                                                                                               <  1:1500619/100‑1 (MQ=255)   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTa                                                                                             <  2:934160/100‑1 (MQ=255)   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGAGGAGGTa                                                                                             >  1:2317348/1‑100 (MQ=255)   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCAAGGAGGTa                                                                                             >  1:2384971/1‑100 (MQ=255)   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGAAATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTa                                                                                             >  2:1596552/1‑100 (MQ=255)    tCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaa                                                                                            >  2:354138/1‑100 (MQ=255)        tttCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTc                                                                                        <  1:1900173/100‑1 (MQ=255)          tCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTg                                                                                      >  1:3008064/1‑100 (MQ=255)           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                     <  2:1915205/100‑1 (MQ=255)           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                     >  2:845202/1‑100 (MQ=255)            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                    >  2:356276/1‑100 (MQ=255)            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                    <  1:2809333/100‑1 (MQ=255)            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                    >  2:3041569/1‑100 (MQ=255)             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                   >  2:2090428/1‑100 (MQ=255)             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                   <  2:1916751/100‑1 (MQ=255)             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                   >  2:2090482/1‑100 (MQ=255)             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                   <  2:301483/100‑1 (MQ=255)             gtgtTCAGTGCTGATAATTGTTTTTGAGCTAAAGGATGTTGAGGATGGCGCACGGTATCAATTAATTTTTTTTCTGTTTTTTTGGAGGTAAATTCTgggg                                                                                   <  2:2535687/100‑1 (MQ=255)               gtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgat                                                                                 >  2:274881/1‑100 (MQ=255)               gtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgan                                                                                 <  1:2384111/100‑2 (MQ=255)                ttCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgat                                                                                 <  1:2485882/99‑1 (MQ=255)                 tCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTTGGgatga                                                                               >  1:1695898/1‑100 (MQ=255)                  cAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgatgat                                                                              >  1:990213/1‑100 (MQ=255)                  cAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgatgat                                                                              <  1:961125/100‑1 (MQ=255)                  cAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAAATAAATTTTgggacag                                                                                >  1:1804330/1‑94 (MQ=255)                          ctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAt                                                                      <  1:2369234/100‑1 (MQ=255)                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCATGATATCAAATAATGCTTTTTCTGTTCTCGAGTAGGTAAATTTTGGGTATTAATGTATAAtc                                                                     >  2:1027711/1‑100 (MQ=255)                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtc                                                                     >  2:2168893/1‑100 (MQ=255)                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtc                                                                     <  2:1614803/100‑1 (MQ=255)                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGAATAAACAAtc                                                                     >  1:2680830/1‑100 (MQ=255)                             aTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtctc                                                                   <  2:412552/100‑1 (MQ=255)                               tGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAc                                                                 <  1:1498086/100‑1 (MQ=255)                                gTTTTTCATCTATATGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCACGGAGGTAAATTCTGGGGATGATTGAACAATCTCAcc                                                                <  2:2685882/100‑1 (MQ=255)                                 tttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCt                                                               <  2:1151556/100‑1 (MQ=255)                                  ttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCta                                                              >  1:2970155/1‑100 (MQ=255)                                   tttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaa                                                             <  2:1299449/100‑1 (MQ=255)                                   tttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACAtaa                                                             >  1:2708765/1‑100 (MQ=255)                                     tCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCCAGGAAGTAAATTCTAGGAAACATTAAACAATTTCACCtaata                                                           >  1:2422021/1‑100 (MQ=255)                                      cATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACTtaataa                                                          >  1:1289206/1‑100 (MQ=255)                                       aTCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTaataat                                                         >  1:2007774/1‑100 (MQ=255)                                         cTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGAGATGATTGAACAATCTCACCTAATAATTc                                                       >  1:1665454/1‑100 (MQ=255)                                         cTAAAGGATGTTGAGAATGGAGCACGATATCAACTAATGCTTTTTCTGCTCTAGCAGAGGTAAATTCTGGGGATGATTGAACAATCTAACCTAATAATTc                                                       >  2:1225136/1‑100 (MQ=255)                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCa                                                      <  1:864119/100‑1 (MQ=255)                                           aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                     <  2:1780357/100‑1 (MQ=255)                                           aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                     <  1:987350/100‑1 (MQ=255)                                             aGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTg                                                   >  1:2738483/1‑100 (MQ=255)                                              ggATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTgg                                                  <  2:2113853/100‑1 (MQ=255)                                                aTGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCa                                                <  2:827547/100‑1 (MQ=255)                                                 tGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCACACCTAATAATTAACTgg                                                  >  1:3002503/1‑97 (MQ=255)                                                 tGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTAGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCAc                                               >  2:1499799/1‑100 (MQ=255)                                                   ttGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACgg                                             >  1:1111275/1‑100 (MQ=255)                                                   ttGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACgg                                             <  2:2985034/100‑1 (MQ=255)                                                    tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGt                                            <  1:93055/100‑1 (MQ=255)                                                     gagaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGTATGATTAAACAATCTCACCTAATAATTCACTGGCACGGtt                                           >  1:1146872/1‑100 (MQ=255)                                                     gagaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGtt                                           >  1:1146873/1‑100 (MQ=255)                                                      agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                          <  2:1076524/100‑1 (MQ=255)                                                      agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                          <  1:982468/100‑1 (MQ=255)                                                       gaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                          <  1:1390307/99‑1 (MQ=255)                                                       gaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCa                                         >  2:2701361/1‑100 (MQ=255)                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGACCAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                      <  1:2144805/100‑1 (MQ=255)                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                      <  1:1699552/100‑1 (MQ=255)                                                           ggCGCATGATATTAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGa                                     <  2:2652166/100‑1 (MQ=255)                                                             cgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAtt                                   <  2:222965/100‑1 (MQ=255)                                                              gcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                                  <  1:2614289/100‑1 (MQ=255)                                                              gcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                                  <  2:2238123/100‑1 (MQ=255)                                                              gcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttn                                  <  1:2975881/100‑2 (MQ=255)                                                              gaaCGATATCAACTAATGCTGTTGCTGCTCTAGCGGAGGTAAATTCTGGGTATGATTGAACAATCTCAACTAATAATTCACTGGCACGGTTCAAtt                                      <  2:2594849/94‑2 (MQ=255)                                                               cACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTa                                 <  2:1561527/100‑1 (MQ=255)                                                                aCGATATCAACTAATGCTTTTTCTGCTCTTGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                                  <  1:2412890/98‑1 (MQ=255)                                                                aCGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTat                                <  2:3001358/100‑1 (MQ=255)                                                                aCGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTan                                <  2:867940/100‑2 (MQ=255)                                                                  gATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatca                              >  2:648191/1‑100 (MQ=255)                                                                  gATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatca                              <  1:384841/100‑1 (MQ=255)                                                                       cAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGcc                         >  1:2407572/1‑100 (MQ=255)                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTCCAATGATTTATCATCGCCATGc                     <  2:1762879/100‑1 (MQ=255)                                                                            aaTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCa                    >  1:476996/1‑100 (MQ=255)                                                                               gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa                 >  2:685556/1‑100 (MQ=255)                                                                               gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa                 <  1:372693/100‑1 (MQ=255)                                                                               gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa                 <  2:931102/100‑1 (MQ=255)                                                                                  ttttCTGCTCTCGCGGATGTAAATTCTGTGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaat              <  1:1799464/100‑1 (MQ=255)                                                                                  ttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaat              >  1:2113104/1‑100 (MQ=255)                                                                                     tatGCACTCTCGGAGGTAAATTCTGGTGATGATTGAACAATCTCACCTAATAATCCTCTGGCAAGGTTCCATGATTTATCATCGCCATGCCGTAaataat           <  2:1503344/98‑1 (MQ=255)                                                                                     tCTGCTCTCGCGGATGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATTGCCATGCAGTAaataat           <  2:2728026/100‑1 (MQ=255)                                                                                     tCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaataat           <  1:2832742/100‑1 (MQ=255)                                                                                      cTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaataata          >  2:2832710/1‑99 (MQ=255)                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTTAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa         <  1:1437275/100‑1 (MQ=255)                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGTCACGGTTCATTGATTTATCATCGCCATGCAGTAAAGAATct         <  2:2863601/100‑2 (MQ=255)                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa         <  2:2335487/100‑1 (MQ=255)                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa         >  1:320085/1‑100 (MQ=255)                                                                                        gCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAt        >  1:450285/1‑100 (MQ=255)                                                                                         ctctCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCACCATGCAGTAAATAATCAtg       <  2:232770/100‑1 (MQ=255)                                                                                           ctcGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtgtg     >  2:1007150/1‑100 (MQ=255)                                                                                             cgcgGAGGTAAATTCTGGGGATGATTGAACAATGTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATGATCATGTGc    >  2:1986914/1‑99 (MQ=255)                                                                                             cgcgGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGcc   >  2:971710/1‑100 (MQ=255)                                                                                              gcgGAGGTAAATTCTGGGGATTATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCCt  <  1:2238195/100‑1 (MQ=255)                                                                                                 |                                                                                                 TTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCCT  >  NC_000913/4360718‑4360910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑