BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	364,024	C→T	100%	R761H (CGC→CAC)	lacZ ←	beta‑D‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,024	0	C	T	98.6%	432.9 / ‑3.3	138	R761H (CGC→CAC)	lacZ	beta‑D‑galactosidase
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (73/61); total (76/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 143 total aligned reads displayed.

GTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCA  >  NC_000913/363927‑364120

gTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACtggtgg                                                                                                <  1:2208880/100‑1 (MQ=255)

 tCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCTTCAATCCACATCTGTGAAAGAAAGCCTGACTggtggt                                                                                               >  2:1698803/1‑100 (MQ=255)

  cGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGtt                                                                                              >  1:1052729/1‑100 (MQ=255)

   gTTATCCAGCGGTGCACGGGTGGACTGAGCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGTAAGCCTGACTGGTGGTTa                                                                                             <  2:2587737/100‑1 (MQ=255)

   gTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTa                                                                                             <  2:2149223/100‑1 (MQ=255)

   gTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTa                                                                                             >  1:617466/1‑100 (MQ=255)

   gTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTa                                                                                             >  1:2365451/1‑100 (MQ=255)

    ttATCCATCGGTGCACTGGTGAACTGCTCGCGCAGTTTCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACGggtggt                                                                                               <  2:77058/97‑1 (MQ=255)

      aTCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAt                                                                                          >  2:1422772/1‑100 (MQ=255)

       tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAtt                                                                                         >  1:2924105/1‑100 (MQ=255)

         cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAttac                                                                                       >  1:1610343/1‑98 (MQ=255)

          aGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCAGATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGcc                                                                                      <  2:1620668/100‑1 (MQ=255)

          aGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGcc                                                                                      <  2:1229340/100‑1 (MQ=255)

          aGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCCGACTGGTGGTTAAATTGcc                                                                                      <  1:2301906/100‑1 (MQ=255)

                cttccgatctaCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCt                                                                                <  2:2440445/90‑1 (MQ=255)

                  aCGGGTGAACTGATCGCGCAGCGGCGTTAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCtta                                                                              <  1:1927643/100‑1 (MQ=255)

                     ggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatta                                                                           >  2:5505/1‑100 (MQ=255)

                     ggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatta                                                                           >  2:3020453/1‑100 (MQ=255)

                     ggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAACAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatta                                                                           <  2:1610324/100‑1 (MQ=255)

                       tGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAcc                                                                         <  2:2883950/100‑1 (MQ=255)

                        gAACTGATCGCGCAGCGGCGTTAGCAGTTGTTTTTTATCGTCAATTCTCATTTGTGAAAGAAAGCCTGACTGGTGGGTAAATTGCCAACGCTTATTAccc                                                                        <  1:827450/100‑1 (MQ=255)

                         ctcTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCa                                                                       <  1:2197268/98‑1 (MQ=255)

                         atcTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCa                                                                       <  2:1838113/98‑1 (MQ=255)

                         aaCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCa                                                                       >  1:2024060/1‑100 (MQ=255)

                         aaCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCa                                                                       >  1:1206828/1‑100 (MQ=255)

                          aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCtagatcggaag                                                                      >  1:2440474/1‑90 (MQ=255)

                          aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGATGGTTAAATTGCCAACGCTTATTACCCAg                                                                      >  2:1038656/1‑100 (MQ=255)

                            tGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCt                                                                    <  2:2323017/100‑1 (MQ=255)

                            tGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCt                                                                    >  2:1730973/1‑100 (MQ=255)

                            tGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCt                                                                    >  2:1730960/1‑100 (MQ=255)

                             gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                   <  1:451846/100‑1 (MQ=255)

                             gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                   >  1:187732/1‑100 (MQ=255)

                             gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                   >  2:1856219/1‑100 (MQ=255)

                              agcgcgCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTTTGAAAGAAAGCTTGACTAGTGGTTAAATTGCAAACGCTTATTACCCAGCTCg                                                                  <  2:319233/98‑1 (MQ=255)

                              aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCg                                                                  >  2:2654803/1‑100 (MQ=255)

                              aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCg                                                                  >  2:2731832/1‑100 (MQ=255)

                              aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACAGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCg                                                                  >  2:2769095/1‑100 (MQ=255)

                               tCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGa                                                                 <  2:2175932/100‑1 (MQ=255)

                                cgcgCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTAACAGGAGGTTAAATTGCCAACGCTTATTACCCAGCTCGAt                                                                >  1:2504563/1‑100 (MQ=255)

                                  cgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGc                                                              <  2:1184325/100‑1 (MQ=255)

                                  cgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGc                                                              <  1:2458168/100‑1 (MQ=255)

                                    cAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCaa                                                            >  1:2938734/1‑100 (MQ=255)

                                    cAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAACCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGcga                                                            >  1:1562457/1‑98 (MQ=255)

                                           gTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCa                                                     >  1:2946729/1‑100 (MQ=255)

                                             cagcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAtt                                                   <  2:2032907/100‑1 (MQ=255)

                                             cagcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAtt                                                   >  1:2524620/1‑100 (MQ=255)

                                             cagcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGAAAAAATCCAtt                                                   <  2:2029679/100‑1 (MQ=255)

                                                 agTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGc                                               <  1:2611756/100‑1 (MQ=255)

                                                   ttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCAAAAAAAAAAAAAAAATCCATTTCGCt                                              <  2:2098632/99‑1 (MQ=255)

                                                    tgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtg                                             >  1:1062516/1‑99 (MQ=255)

                                                    tgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCAAAAAAAAAAAAAAAATCCATTTCGCtgg                                            <  2:144670/100‑1 (MQ=255)

                                                     gttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggt                                           >  1:2351837/1‑100 (MQ=255)

                                                      ttttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtg                                          <  2:824715/100‑1 (MQ=255)

                                                      ttttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtg                                          >  1:1591196/1‑100 (MQ=255)

                                                      ttttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGAGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtg                                             >  2:2726207/1‑97 (MQ=255)

                                                       tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTTGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                         <  1:105485/100‑1 (MQ=255)

                                                       tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                         >  1:2139715/1‑100 (MQ=255)

                                                       tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTAATTACCCAGCTCGATGCAAAAATCTATTTCGCtggtgg                                         <  2:1870590/100‑1 (MQ=255)

                                                        ttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                         <  2:904614/99‑1 (MQ=255)

                                                         tttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTggtggt                                        >  1:2881368/1‑99 (MQ=255)

                                                         tttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTc                                       >  1:2876835/1‑100 (MQ=255)

                                                           tATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAg                                     >  2:2814793/1‑100 (MQ=255)

                                                           tATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAg                                     >  1:1341032/1‑100 (MQ=255)

                                                           tATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAg                                     >  1:1530943/1‑100 (MQ=255)

                                                            aTCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAg                                     >  1:696179/1‑99 (MQ=255)

                                                              cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATg                                  <  2:1676673/100‑1 (MQ=255)

                                                               gCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGc                                 >  1:982474/1‑100 (MQ=255)

                                                                 cAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCgg                               >  1:392580/1‑100 (MQ=255)

                                                                  aaTCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                              <  2:2265863/100‑1 (MQ=255)

                                                                  aaTCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                              <  1:1221384/100‑1 (MQ=255)

                                                                   aTCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCgg                               <  1:444614/98‑1 (MQ=255)

                                                                    tCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGAt                            >  1:2470501/1‑100 (MQ=255)

                                                                      cacaTCTGTGAAAGAAAGCCTGACTTGTGGTTAAATTGCCAACTCTGATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATgg                          <  2:2391121/100‑1 (MQ=255)

                                                                      cacaTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATgg                          >  1:708224/1‑100 (MQ=255)

                                                                      cacaTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATgg                          <  1:2942896/100‑1 (MQ=255)

                                                                       acaTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGc                         <  1:1422089/100‑1 (MQ=255)

                                                                          tCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTg                      >  2:165912/1‑100 (MQ=255)

                                                                          tCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATTCATTTCGCTGGTGGTCAGATGCGGGATGGCGTg                      >  2:2431932/1‑100 (MQ=255)

                                                                           cTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCTATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTgg                     <  1:2094723/100‑1 (MQ=255)

                                                                           cTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTgg                     >  2:1089063/1‑100 (MQ=255)

                                                                             gtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGa                   >  2:709879/1‑100 (MQ=255)

                                                                              tgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAc                  <  1:2922969/100‑1 (MQ=255)

                                                                              tgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATACATTTCACTGGTGGTCAGATGCGGGATGGCGTGGGAc                  >  2:1813243/1‑100 (MQ=255)

                                                                               gaaagaaaGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcg                 >  1:1757739/1‑100 (MQ=255)

                                                                               gaaagaaaGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcg                 >  1:571287/1‑100 (MQ=255)

                                                                               gaaagaaaGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcg                 >  1:2899426/1‑100 (MQ=255)

                                                                                aaagaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgc                >  1:1812094/1‑100 (MQ=255)

                                                                                aaagaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgc                >  2:2069041/1‑100 (MQ=255)

                                                                                aaagaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGAGGACAGATGCGGGATGGCGTGGGAcgc                >  2:860416/1‑100 (MQ=255)

                                                                                     aagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGcggcgg           <  1:165914/100‑1 (MQ=255)

                                                                                     aagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGcggcgg           <  1:2731864/100‑1 (MQ=255)

                                                                                     aagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGcggcgg           <  1:303804/100‑1 (MQ=255)

                                                                                      agCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCggg          <  1:1945977/100‑1 (MQ=255)

                                                                                      agCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCggg          <  2:911631/100‑1 (MQ=255)

                                                                                         cTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAg       <  2:796388/100‑1 (MQ=255)

                                                                                         cTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAg       <  1:1274674/100‑1 (MQ=255)

                                                                                         cTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTAAGATGCGGGATGGCGTGGGACGCGGCGGGGAg       >  1:296582/1‑100 (MQ=255)

                                                                                             cTGGTGGTTAAGTTGCCAACTTTTATTACCAAAAAAAAAAAAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg     <  2:2106221/98‑1 (MQ=255)

                                                                                              tggtggTTAAATTGCCAATGCTTATTTTTCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg     <  1:337632/97‑1 (MQ=255)

                                                                                              tggtggTTAAATTGCCAACGCTTAGTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTca  <  2:439544/100‑1 (MQ=255)

GTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCA  >  NC_000913/363927‑364120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`