Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,615,859 T→G 100% T403P (ACC→CCC)  yjjI ← DUF3029 family protein, putative glycine radical enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,615,8590TG99.2% 389.1 / ‑4.3 119T403P (ACC→CCC) yjjIDUF3029 family protein, putative glycine radical enzyme
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (60/58);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

Only 100 of 121 total aligned reads displayed.

CTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTT  >  NC_000913/4615764‑4615954
                                                                                               |                                                                                               
cTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATc                                                                                             >  2:1581173/1‑100 (MQ=255)
  gCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCgg                                                                                           >  1:706801/1‑100 (MQ=255)
  gCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCgg                                                                                           >  1:1152244/1‑100 (MQ=255)
     ggTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGggatcggatc                                                                                        >  2:2056651/1‑100 (MQ=255)
     ggTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGggatcggatc                                                                                        >  2:1719126/1‑100 (MQ=255)
      gTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCt                                                                                       <  1:884565/100‑1 (MQ=255)
       ttACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGTGGCGACAGTTTGCAGATGGGGGATCGGATCTg                                                                                      <  2:890350/100‑1 (MQ=255)
       ttACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTg                                                                                      <  2:1513299/100‑1 (MQ=255)
        tACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGACGGAATAATAATAAGCATGTTGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTgg                                                                                     >  2:1652290/1‑100 (MQ=255)
         aCGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGc                                                                                    <  2:2276833/100‑1 (MQ=255)
         aCGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGc                                                                                    <  1:1292994/100‑1 (MQ=255)
          cGTTTGATGGTTTCGTCGACCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGAAGATGGAGGGTCGGAtattg                                                                                     >  2:195116/1‑94 (MQ=255)
           gTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTc                                                                                  <  2:2806650/100‑1 (MQ=255)
           gTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTc                                                                                  >  1:417913/1‑100 (MQ=255)
            tttGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCa                                                                                 <  1:2972388/100‑1 (MQ=255)
             ttGATGGTTTAGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCAt                                                                                >  2:2675745/1‑100 (MQ=255)
               gATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCg                                                                              >  1:1495196/1‑100 (MQ=255)
               gATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCg                                                                              >  2:840505/1‑100 (MQ=255)
                aTGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGc                                                                             <  1:1077488/100‑1 (MQ=255)
                 tGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGcc                                                                            <  2:571947/100‑1 (MQ=255)
                  ggTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCa                                                                           >  2:834098/1‑100 (MQ=255)
                   ttttCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCAt                                                                          >  2:1370828/2‑100 (MQ=255)
                   ggttaaacgAACGGCAGAATGTCGATGATTACGAATGAATAATAAGCATGATGCGGGGCTACAGTGTGCAGATGTGGAATCGGATCTGGGTCATCGcga                                                                           <  2:233021/92‑3 (MQ=255)
                   gTTTCGTCGAGCGTCAGAATGTCGCTGATGACGAAATAATGATAAGCATGATGCGGCGCGAAAGTTTGCAGCTGCTGGATCGGATCTGGCTCATCGca                                                                            >  2:2467627/1‑97 (MQ=255)
                       cgtcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACgg                                                                      >  1:2306440/1‑100 (MQ=255)
                       cgtcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACgg                                                                      <  2:2715004/100‑1 (MQ=255)
                       cgtcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATAGGATCTGGCTCATCGCCATACgg                                                                      >  1:1839253/1‑100 (MQ=255)
                       cgtcgAGCGTCAGAATGTCGCAGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACgg                                                                      >  2:1314476/1‑100 (MQ=255)
                         tcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCa                                                                    >  2:2986477/1‑100 (MQ=255)
                           gAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACggagat                                                                  >  1:459499/1‑96 (MQ=255)
                             gCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAAcg                                                                <  1:1724583/100‑1 (MQ=255)
                               gTCAGAATGTCGCTGATGCAGGAATAATAATAAGCATGATGCGGTTCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAAcgcg                                                              <  2:978052/100‑1 (MQ=255)
                                tCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAAcgcgc                                                             >  2:2378135/1‑100 (MQ=255)
                                tCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAAcgcgc                                                             <  1:320768/100‑1 (MQ=255)
                                  aGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGCTCTGGCTCATCGCCATACGGCAAACgcgcgc                                                           >  1:2249638/1‑100 (MQ=255)
                                  aGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAAcgcgc                                                             <  2:2440532/98‑1 (MQ=255)
                                  aGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACgcgcgc                                                           <  1:2855215/100‑1 (MQ=255)
                                     aTGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCg                                                        >  1:3006398/1‑100 (MQ=255)
                                      tGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCgg                                                       >  1:2140424/1‑100 (MQ=255)
                                       gTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGc                                                      <  2:1184141/100‑1 (MQ=255)
                                         cGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCATATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCgt                                                    <  2:915363/100‑1 (MQ=255)
                                          gCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCgtg                                                   >  2:1559942/1‑100 (MQ=255)
                                          gCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCgtg                                                   >  2:2886601/1‑100 (MQ=255)
                                            tGATGTCGGAATAATAATAAGCATGATGCGGGGCTACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCgtggt                                                 <  1:1292852/100‑1 (MQ=255)
                                              aTGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGc                                               <  2:2618830/100‑1 (MQ=255)
                                              aTGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGc                                               <  1:2885947/100‑1 (MQ=255)
                                               tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGTTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc                                              <  2:73099/100‑1 (MQ=255)
                                               tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc                                              >  1:654139/1‑100 (MQ=255)
                                               tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc                                              <  2:2719922/100‑1 (MQ=255)
                                               tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc                                              >  1:2145399/1‑100 (MQ=255)
                                                gCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCg                                             <  1:583084/100‑1 (MQ=255)
                                                 ccGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGa                                            >  2:399455/1‑100 (MQ=255)
                                                 ccGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGa                                            <  1:2647701/100‑1 (MQ=255)
                                                 ccGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGACATAAGGCAAACGAGCGCCCGGCGTGGTGACGa                                            >  1:620742/1‑100 (MQ=255)
                                                  nggAATAATAATAAGCATGATGCAGGGCGACAGTTTGCAGATGGGGGATCGGATATGGCGCATCACCATACGGCAAACGCGCGCCCGGCGAGGTGCCg                                             >  2:708603/2‑98 (MQ=255)
                                                  cGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGat                                           >  1:147169/1‑100 (MQ=255)
                                                    gAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGatat                                         >  2:3037439/1‑100 (MQ=255)
                                                     aataataatAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATc                                        <  1:474689/100‑1 (MQ=255)
                                                      ataataataAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCg                                       <  1:2108668/100‑1 (MQ=255)
                                                       taataataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCgg                                      <  1:2253084/100‑1 (MQ=255)
                                                       taataataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCgg                                      <  1:1723325/100‑1 (MQ=255)
                                                       taataataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCgg                                      <  2:2160192/100‑1 (MQ=255)
                                                          taataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAAc                                   <  1:2826634/100‑1 (MQ=255)
                                                           aataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGTGTGGTGACGATATCGGAACt                                  <  1:2064262/100‑1 (MQ=255)
                                                           aataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACt                                  <  1:2129722/100‑1 (MQ=255)
                                                             taaGCATGATGCGGGGTGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCAGATATCGGAACt                                  <  2:1127067/98‑1 (MQ=255)
                                                              aaGCATTAGGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGAt                               <  1:2482034/100‑1 (MQ=255)
                                                               aGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATc                              >  1:1205561/1‑100 (MQ=255)
                                                               aGCATGATGCGGAGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACTGCAAAAGCGCGCCCGGCGTGGTGCCGATATAGGAACTGa                                >  2:1519099/1‑98 (MQ=255)
                                                                gCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCCTACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcc                             >  2:687685/1‑100 (MQ=255)
                                                                gCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGACGATATCGGAAATGATcc                             >  1:2955877/1‑100 (MQ=255)
                                                                 cATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATccc                            <  2:407870/100‑1 (MQ=255)
                                                                  atgatgCGGGGCGACAGTTTGCAGATGGGGGTTCGGATTTGGTTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcccc                           <  1:1231294/100‑1 (MQ=255)
                                                                  atgatgCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcccc                           >  1:2392473/1‑100 (MQ=255)
                                                                  atgatgCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcccc                           <  1:1401719/100‑1 (MQ=255)
                                                                   tgatgCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCg                          <  1:2603620/100‑1 (MQ=255)
                                                                     atgCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGAGCCCCGAc                        <  1:1581745/100‑1 (MQ=255)
                                                                      tgCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACt                       >  2:57407/1‑100 (MQ=255)
                                                                       gCGGGGCGACAGTTTGCAGATGGGGGATTGGAGCTGGCTCATCGCCATACGGGAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGAc                        <  2:466240/98‑1 (MQ=255)
                                                                       gCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtg                      <  2:1189033/100‑1 (MQ=255)
                                                                         ggggCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtgtg                    <  1:2910584/100‑1 (MQ=255)
                                                                         ggggCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtgtg                    <  1:656035/100‑1 (MQ=255)
                                                                         ggggCGACAGTTTGCAGATGGGGGATCAGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtgtg                    <  1:115177/100‑1 (MQ=255)
                                                                           ggCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCg                  <  1:2324733/100‑1 (MQ=255)
                                                                            gCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgt                 >  2:2298131/1‑100 (MQ=255)
                                                                            gCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCACGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgt                 >  2:2617456/1‑100 (MQ=255)
                                                                             cGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgtg                >  2:541122/1‑100 (MQ=255)
                                                                              gACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgtgt               <  2:63537/100‑1 (MQ=255)
                                                                               aCAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATACCCGACTGTGCGTGTa              >  1:2403464/1‑100 (MQ=255)
                                                                                cAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTaa             >  2:105676/1‑100 (MQ=255)
                                                                                 aGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAAc            <  1:2412269/100‑1 (MQ=255)
                                                                                 aGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAAc            >  2:2961480/1‑100 (MQ=255)
                                                                                   tttGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACAt          <  1:243552/100‑1 (MQ=255)
                                                                                   tttGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACAt          >  2:2543572/1‑100 (MQ=255)
                                                                                     tGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCGCGGAGAGGTGCCGATATCGAAACTGATCCCCGACTGTGCGTGTAACATgg        >  1:2427215/1‑100 (MQ=255)
                                                                                     tGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATgg        >  2:426065/1‑100 (MQ=255)
                                                                                     tGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAAATGATCCCCGACTGTGCGTGTAACATgg        >  1:1915971/1‑100 (MQ=255)
                                                                                       cAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGCCCGTGCGTGTAACATGccg      >  2:3037137/1‑100 (MQ=255)
                                                                                       cAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGAAACTGATCCCCGACTGTGCGTGTAACATGgcg      >  2:591426/1‑100 (MQ=255)
                                                                                           tGGGGGATCGGATCTGGCTCATCACCATACGGCAAACGCGCGCCCGGCGTGGTGCCGCTATCGGAACTGATCCCCGCCTGTGCGTGTAACATGgcgc     >  2:1687657/1‑97 (MQ=255)
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CTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTT  >  NC_000913/4615764‑4615954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: