Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 367,830 G→A 100% Y197Y (TAC→TAT mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913367,8300GA99.3% 446.9 / ‑4.9 135Y197Y (TAC→TATmhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (79/55);  total (80/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 135 total aligned reads displayed.

CCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTC  >  NC_000913/367733‑367925
                                                                                                 |                                                                                               
  aGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAAc                                                                                             <  2:2027160/100‑1 (MQ=255)
   gtttCAGACAGCCAATCAACCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAAcc                                                                                            >  2:1475417/3‑100 (MQ=255)
   gATTCAGACAGTCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAAcc                                                                                            <  2:910611/100‑1 (MQ=255)
     ttCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCct                                                                                          >  2:1213708/1‑100 (MQ=255)
         gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCttt                                                                                      >  2:2295393/1‑100 (MQ=255)
         gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCttt                                                                                      >  2:1423477/1‑100 (MQ=255)
          aCAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTg                                                                                     <  1:345894/100‑1 (MQ=255)
             gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgc                                                                                  >  2:1640661/1‑100 (MQ=255)
             gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgc                                                                                  >  1:1646640/1‑100 (MQ=255)
              ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcg                                                                                 >  1:779956/1‑100 (MQ=255)
               cAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgc                                                                                >  1:1414766/1‑100 (MQ=255)
               cAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTAATACCAGCCAAGGTAGTTATGTCCATAACCCTCTTTgcgcg                                                                                 >  1:1040285/1‑99 (MQ=255)
                aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcg                                                                               >  1:1845203/1‑100 (MQ=255)
                aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcg                                                                               >  1:3037386/1‑100 (MQ=255)
                   cACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcg                                                                             >  2:1466203/1‑99 (MQ=255)
                    aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCATCCGCAGTAATTCTGTCCATAACCCTCTTTgcgcgcg                                                                               >  1:1351232/1‑96 (MQ=255)
                    aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcgcg                                                                           <  1:2506557/100‑1 (MQ=255)
                     cccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcgcgc                                                                          >  1:783943/1‑100 (MQ=255)
                      ccGTTGTTCACTGCGCAGCTGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGcc                                                                         <  1:1420526/100‑1 (MQ=255)
                      ccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGcc                                                                         >  2:1521233/1‑100 (MQ=255)
                      ccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGcc                                                                         <  1:2936109/100‑1 (MQ=255)
                        gttgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAg                                                                       <  2:1601887/100‑1 (MQ=255)
                        gatgttCACTGCGAAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAg                                                                       >  1:2048631/3‑100 (MQ=255)
                           gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAAt                                                                    >  2:465452/1‑100 (MQ=255)
                           gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCACAGCCGCGGTAGTTGTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGaa                                                                     >  2:2168152/1‑99 (MQ=255)
                            ttCACTGCGCAGCGGTCCGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCGATAACCTTCTTTGCGCGCGCGCGCCAGAATg                                                                   <  2:363451/100‑1 (MQ=255)
                             tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATgg                                                                  >  2:203001/1‑100 (MQ=255)
                             tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATgg                                                                  >  2:2691943/1‑100 (MQ=255)
                             tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATgg                                                                  >  1:1654661/1‑100 (MQ=255)
                             tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATgg                                                                  <  1:2203823/100‑1 (MQ=255)
                              cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  2:2315592/1‑100 (MQ=255)
                              cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  1:927244/1‑100 (MQ=255)
                              cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  1:2188636/1‑100 (MQ=255)
                               aCTGCGCAGCGGTACGGCGATAGAGGCGATTTTCTCCTCCTCATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGTGCCAGAATGGCt                                                                <  2:1323696/100‑1 (MQ=255)
                               aCTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCt                                                                >  1:3017240/1‑100 (MQ=255)
                                cTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCtt                                                               >  1:2273154/1‑100 (MQ=255)
                                     cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGc                                                          <  2:678564/100‑1 (MQ=255)
                                     cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGc                                                          >  1:2167285/1‑100 (MQ=255)
                                     cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGc                                                          >  1:159040/1‑100 (MQ=255)
                                         ggTACGTCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTa                                                      <  1:809967/100‑1 (MQ=255)
                                         ggTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATCACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTa                                                      <  2:1654641/100‑1 (MQ=255)
                                         ggTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTa                                                      >  2:3009163/1‑100 (MQ=255)
                                          gTACGGCGATAGAGGCGATCTTCTTCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGTGCGCCAGAATGGCTTCCAGc                                                          <  2:2675530/95‑1 (MQ=255)
                                           tACGGCGATAGAGGCGATCTTCTCTTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                    <  1:618683/100‑1 (MQ=255)
                                           tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                    <  1:2097798/100‑1 (MQ=255)
                                            aCGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACg                                                   >  1:1103670/1‑100 (MQ=255)
                                            aCGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACg                                                   <  2:2402778/100‑1 (MQ=255)
                                            aCGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACg                                                   <  2:2267727/100‑1 (MQ=255)
                                             cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCTTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                  <  2:1651687/100‑1 (MQ=255)
                                             cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                  >  1:2486504/1‑100 (MQ=255)
                                              ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                  >  2:1835159/1‑99 (MQ=255)
                                                 gATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTcc                                              <  1:2993470/100‑1 (MQ=255)
                                                     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          <  1:2943970/100‑1 (MQ=255)
                                                     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          <  1:2401949/100‑1 (MQ=255)
                                                     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGAGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGt                                           >  1:941750/1‑99 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCATCCGCGGTATTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        <  1:2728007/100‑1 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        >  2:112336/1‑100 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        <  2:779952/100‑1 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        >  2:2208637/1‑100 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        >  2:2581611/1‑100 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGAGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                         >  2:152024/1‑99 (MQ=255)
                                                         cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAGTGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                      <  2:1265813/100‑1 (MQ=255)
                                                            ttttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCTATATCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCTGTTTTAGCGGTTCCCGTGCCAGtt                                    <  2:2704663/97‑1 (MQ=255)
                                                            tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTg                                   >  2:3045806/1‑100 (MQ=255)
                                                            tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTg                                   <  2:2074590/100‑1 (MQ=255)
                                                            tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTGTGCGCTCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                    <  2:2473630/99‑1 (MQ=255)
                                                             cttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGa                                  <  2:579799/100‑1 (MQ=255)
                                                                ctcctcCTGCTCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAg                               <  1:2163842/100‑1 (MQ=255)
                                                                ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTACAGCTTTAAAGGTTCACGTGATAGTTGATAg                               >  1:3025220/1‑100 (MQ=255)
                                                                 tcctccTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAg                               <  2:2282139/99‑1 (MQ=255)
                                                                 tcctccTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGACAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGt                              >  1:110230/1‑100 (MQ=255)
                                                                   ctcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCTCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtca                            <  1:328876/100‑1 (MQ=255)
                                                                   ctcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtca                            >  1:2719926/1‑100 (MQ=255)
                                                                    tcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcat                           >  1:1977889/1‑100 (MQ=255)
                                                                     cctGATCTCAGCCTCGGTAGTTCTGTCCATAACCCTTTTTGCGCGCGCGCGCCAGAATTGTTTCCAGCTTTATCGGTTCCCGTGCCAGTTGATAGtcatc                          <  2:496554/100‑1 (MQ=255)
                                                                     cctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                          >  2:2142347/1‑100 (MQ=255)
                                                                     cctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                          >  1:1955518/1‑100 (MQ=255)
                                                                     cctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                          >  1:2709957/1‑100 (MQ=255)
                                                                     cctGATCCCAGCCGCGCTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                          <  2:1766293/100‑1 (MQ=255)
                                                                      ctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatca                         >  2:242025/1‑100 (MQ=255)
                                                                       tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTACCGGTTCCCGTGCCAGTTGATAGTCATCAc                        >  2:1372740/1‑100 (MQ=255)
                                                                       tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                        >  1:1810779/1‑100 (MQ=255)
                                                                          tCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgg                     <  2:1265392/100‑1 (MQ=255)
                                                                           cccAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCggg                    >  1:1770642/1‑100 (MQ=255)
                                                                           cccAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCggg                    >  1:2178767/1‑100 (MQ=255)
                                                                            ccAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGACTTCCAGCTTTAACGGTTACCGTGCCAGTTGATAGTCATCACCggg                    >  1:1202995/1‑99 (MQ=255)
                                                                              aGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                 <  2:3037352/100‑1 (MQ=255)
                                                                               gCCGCGGTAGTTCTGTCCATAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa              >  2:441823/1‑100 (MQ=255)
                                                                                 cgcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa              <  1:670277/100‑1 (MQ=255)
                                                                                  gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTGCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg             >  2:1595891/1‑100 (MQ=255)
                                                                                  gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg             <  1:1624090/100‑1 (MQ=255)
                                                                                    ggTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGc           <  1:2111908/100‑1 (MQ=255)
                                                                                    ggTAGTTCTGTCCATAACCCTCTAAGTCCGCGCGAGACAGAATGGCTTACAGCTTTAACGGTTCCCGTTCCAGTTTATATTCATAAACGGGGCggg               <  2:1598890/96‑1 (MQ=255)
                                                                                       aGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTaa        >  2:712697/1‑100 (MQ=255)
                                                                                       aGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTaa        >  1:12121/1‑100 (MQ=255)
                                                                                        gTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc       >  2:1374554/1‑100 (MQ=255)
                                                                                         ttCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACa      <  1:528471/100‑1 (MQ=255)
                                                                                           cTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAAAtt    >  2:2340039/1‑100 (MQ=255)
                                                                                             gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAtt    >  2:1796890/1‑98 (MQ=255)
                                                                                             gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc  <  2:2777251/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTC  >  NC_000913/367733‑367925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: