Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 400,214 T→C 100% D237G (GAC→GGC)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,2140TC99.0% 350.9 / ‑4.8 102D237G (GAC→GGC) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (53/48);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

Only 100 of 103 total aligned reads displayed.

ACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTG  >  NC_000913/400118‑400310
                                                                                                |                                                                                                
aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGt                                                                                               >  2:2599143/1‑100 (MQ=255)
    cTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGcc                                                                                           >  1:2019360/1‑100 (MQ=255)
    cTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGcc                                                                                           >  2:1639739/1‑100 (MQ=255)
      ttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGCACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCa                                                                                         >  2:1942821/1‑100 (MQ=255)
        cgcgCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGa                                                                                       >  2:86088/1‑100 (MQ=255)
         gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGaa                                                                                      >  1:1205442/1‑100 (MQ=255)
          cgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAAc                                                                                     <  1:1658456/100‑1 (MQ=255)
          cgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAAc                                                                                     <  1:2534378/100‑1 (MQ=255)
          cgcCATCTTCGTCAATGTACTTGGAATCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGAGCTGGCTTGCAGATTGCCGTTGCCCAGa                                                                                       >  2:1888966/1‑98 (MQ=255)
           gcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACt                                                                                    >  1:2442747/1‑100 (MQ=255)
            ccATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTg                                                                                   >  2:2046683/1‑100 (MQ=255)
             cATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCAAAAACTgc                                                                                  >  1:1475723/1‑100 (MQ=255)
               tCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcgc                                                                                >  1:786211/1‑100 (MQ=255)
               tCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcgc                                                                                <  1:1901098/100‑1 (MQ=255)
                cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCa                                                                               >  1:1869694/1‑100 (MQ=255)
                   cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                            >  1:1553794/1‑100 (MQ=255)
                   cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                            >  2:61678/1‑100 (MQ=255)
                   cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                            <  1:1852049/100‑1 (MQ=255)
                   cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                            >  2:420928/1‑100 (MQ=255)
                     tCAATTTACTTTGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                            <  1:2545508/98‑1 (MQ=255)
                     tCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGa                                                                          >  2:1448593/1‑100 (MQ=255)
                      cAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAt                                                                         <  1:2349451/100‑1 (MQ=255)
                        aTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtct                                                                       >  2:2735670/1‑100 (MQ=255)
                          gTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCa                                                                     <  1:2638212/100‑1 (MQ=255)
                          gTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCa                                                                     >  1:370340/1‑100 (MQ=255)
                           tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                    >  1:1928328/1‑100 (MQ=255)
                           tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                    >  1:1525345/1‑100 (MQ=255)
                            aCTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACg                                                                   <  1:565321/100‑1 (MQ=255)
                              ttGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGAc                                                                 <  1:1571836/100‑1 (MQ=255)
                               tGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGAcc                                                                >  2:2966662/1‑100 (MQ=255)
                                ggTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCt                                                               <  1:552900/100‑1 (MQ=255)
                                 gtgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCtt                                                              <  2:150032/100‑1 (MQ=255)
                                  tgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                             <  2:542953/100‑1 (MQ=255)
                                   gtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTg                                                            <  2:619295/100‑1 (MQ=255)
                                     cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGAt                                                          >  1:841085/1‑100 (MQ=255)
                                      gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAAAACTGCGCATTCGATCTCACGACCTTTGATc                                                         <  2:2404300/100‑1 (MQ=255)
                                       tAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATcc                                                        <  1:1589201/100‑1 (MQ=255)
                                         ggCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                                      <  2:2354079/100‑1 (MQ=255)
                                         ggCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCt                                                      >  1:2109796/1‑100 (MQ=255)
                                          gCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCtt                                                     <  1:1398551/100‑1 (MQ=255)
                                          gCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCtt                                                     <  1:1119005/100‑1 (MQ=255)
                                          gCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCtt                                                     >  2:1265280/1‑100 (MQ=255)
                                           cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                    >  1:2784923/1‑100 (MQ=255)
                                            aTAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                   >  1:2536175/1‑100 (MQ=255)
                                               gAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCa                                                >  2:675152/1‑100 (MQ=255)
                                                  tttggtGGTGAGTAGGATCTCTCCCCATGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACGGCGCATTCGATCTCGCTATCTTTTTTACCTTGGTCAACg                                             <  2:2000549/95‑1 (MQ=255)
                                                  aTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGGTTGCGGATTGCCGTTGCCCAGGACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCagc                                              <  2:1369515/99‑3 (MQ=255)
                                                  aTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACg                                             <  2:1928305/100‑1 (MQ=255)
                                                   tCGCTGGTGGGTACGATCTCTCCACAGGTGCTGGTTTGCTGATTGCCGTTGCCCACAACTGCGCATTCGCTCTCACGACCTTTGATCACTTGCTCaa                                               >  2:2122162/1‑97 (MQ=255)
                                                     gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                          <  2:362183/100‑1 (MQ=255)
                                                       tGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCAc                                        <  2:3020076/100‑1 (MQ=255)
                                                       tGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCAc                                        >  1:2416087/1‑100 (MQ=255)
                                                        ggTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACt                                       >  1:2451182/1‑100 (MQ=255)
                                                         gTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACtt                                      <  2:50326/100‑1 (MQ=255)
                                                           gAGTACGATCTCTCCCCAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                    <  2:2536144/100‑1 (MQ=255)
                                                           gAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGAACTTTGATCCCTTGCTCAACGATTACttt                                     >  1:702791/1‑99 (MQ=255)
                                                            aGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                                   >  1:2771108/1‑100 (MQ=255)
                                                            aGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                                   >  1:998204/1‑100 (MQ=255)
                                                             gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                    <  1:1018750/98‑1 (MQ=255)
                                                             gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                                  >  1:2338743/1‑100 (MQ=255)
                                                             gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                                  >  1:2015245/1‑100 (MQ=255)
                                                              tACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGa                                 <  2:3032082/100‑1 (MQ=255)
                                                                cGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                               >  1:399457/1‑100 (MQ=255)
                                                                cGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                               >  1:2239574/1‑100 (MQ=255)
                                                                cGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                               <  2:2173974/100‑1 (MQ=255)
                                                                cGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                               >  2:3034682/1‑100 (MQ=255)
                                                                cGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAt                                <  1:117190/99‑1 (MQ=255)
                                                                 gATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                              <  1:1831123/100‑1 (MQ=255)
                                                                  aTCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcga                             >  1:1706572/1‑100 (MQ=255)
                                                                  aTCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                              <  1:2465955/99‑1 (MQ=255)
                                                                    ctctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                           <  2:1580611/100‑1 (MQ=255)
                                                                    ctctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                           >  2:2482207/1‑100 (MQ=255)
                                                                    ctctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                           <  1:2184553/100‑1 (MQ=255)
                                                                    ctctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACAAACTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaac                           >  1:792546/1‑100 (MQ=255)
                                                                     tctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                          >  2:1128219/1‑100 (MQ=255)
                                                                     tctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTAACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                          >  2:1565837/1‑100 (MQ=255)
                                                                      ctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactc                         >  2:1575897/1‑100 (MQ=255)
                                                                      ctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactc                         <  2:1976456/100‑1 (MQ=255)
                                                                      ctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCACAAGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactc                         >  1:52109/1‑100 (MQ=255)
                                                                       tcaaaatgTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCACTCGATCTCACGACCCTTGATCCCTTGCTCAACGATCACTTTATAAtcgaact                          <  2:147952/91‑1 (MQ=255)
                                                                         cacaGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaa                      >  1:1473541/1‑100 (MQ=255)
                                                                          acaGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaac                     <  2:1144523/100‑1 (MQ=255)
                                                                             ggTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcc                  <  1:3043790/100‑1 (MQ=255)
                                                                               tGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAg                <  1:983399/100‑1 (MQ=255)
                                                                                gCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGa               <  1:2641444/100‑1 (MQ=255)
                                                                                gCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGa               >  1:2492394/1‑100 (MQ=255)
                                                                                 cTGGCTTGCGGATTGCCGTTGCTCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt              <  1:1055504/100‑1 (MQ=255)
                                                                                 cTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt              <  1:710614/100‑1 (MQ=255)
                                                                                 cTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGAt              >  1:1881372/1‑100 (MQ=255)
                                                                                  tGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATc             <  1:2290580/100‑1 (MQ=255)
                                                                                  tGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATc             <  2:2239551/100‑1 (MQ=255)
                                                                                     cTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGAc          <  1:1319012/100‑1 (MQ=255)
                                                                                      ttGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACg         <  1:2966695/100‑1 (MQ=255)
                                                                                      tcttccgatctCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACg         <  1:1580871/90‑1 (MQ=255)
                                                                                       tGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACgg        <  1:470772/100‑1 (MQ=255)
                                                                                       tGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACgg        <  2:794027/100‑1 (MQ=255)
                                                                                       tGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACg         >  2:726690/1‑99 (MQ=255)
                                                                                        gCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGc       <  2:2451153/100‑1 (MQ=255)
                                                                                         cGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCa      >  2:144142/1‑100 (MQ=255)
                                                                                             ttGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTg  <  1:1971460/100‑1 (MQ=255)
                                                                                                |                                                                                                
ACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTG  >  NC_000913/400118‑400310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑