Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 428,188 G→T 100% R181L (CGC→CTC)  secD → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913428,1880GT97.1% 379.2 / 1.1 127R181L (CGC→CTC) secDSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base G (0/5);  new base T (49/71);  total (50/77)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

AAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGG  >  NC_000913/428092‑428283
                                                                                                |                                                                                               
aaaaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCAGCACTTTCctcg                                                                                               <  2:318428/99‑1 (MQ=255)
   atatCGATAGCCTACGCAGTGACCTGCGCCAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATg                                                                                            <  2:1804583/99‑1 (MQ=255)
    tatCGATAGCCTACGCAGTTACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGc                                                                                           <  1:608366/99‑1 (MQ=255)
      tCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTa                                                                                         <  2:1970266/99‑1 (MQ=255)
       agATAGCCTACGCAGTGACATGAGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCAATTCTaaa                                                                                       >  2:1001453/2‑100 (MQ=255)
         aTAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTACAGAAAACAACTACGGCCTGAGCATCCCTTTCCTCGATGCTAAAGc                                                                                     >  2:2305508/1‑100 (MQ=255)
         aTAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAgn                                                                                     <  2:784151/100‑2 (MQ=255)
          tAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCt                                                                                    <  2:2546610/100‑1 (MQ=255)
          tAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCt                                                                                    <  1:1418025/100‑1 (MQ=255)
           aGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTc                                                                                   <  2:1891296/100‑1 (MQ=255)
            gCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCg                                                                                  <  1:2086918/100‑1 (MQ=255)
            gCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCg                                                                                  >  2:1224593/1‑100 (MQ=255)
            gCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACCTTCCTCGATGCTAAAGCTCg                                                                                  <  2:1858520/100‑1 (MQ=255)
             ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCTTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGt                                                                                 >  2:2005423/1‑100 (MQ=255)
             ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGt                                                                                 >  2:1086098/1‑100 (MQ=255)
             ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGt                                                                                 >  1:792972/1‑100 (MQ=255)
             ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGt                                                                                 <  2:293788/100‑1 (MQ=255)
              cTACGCAGTGACCTGCGCGAAAAGTGCATCCCTTATATCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg                                                                                <  2:1059578/100‑1 (MQ=255)
              cTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg                                                                                <  1:174462/100‑1 (MQ=255)
              cTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg                                                                                <  2:2284759/100‑1 (MQ=255)
               tACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga                                                                               <  1:409264/100‑1 (MQ=255)
                   cAGTTACCTGCGCGAAAAACGAATCCCGTATAAAAATGTTCTTAAAGAAAACAACTAAGGCATGAACATCACTTTACTCGATGCTAAAGCTGGtgataa                                                                            >  2:1290433/1‑97 (MQ=255)
                   cAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTACTCGATGCTAAAGCTCGTGATGaa                                                                           >  1:1827464/1‑100 (MQ=255)
                     gTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGc                                                                         <  1:1418985/100‑1 (MQ=255)
                     gTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGc                                                                         >  1:1650355/1‑100 (MQ=255)
                         ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt                                                                     >  2:295881/1‑100 (MQ=255)
                         ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt                                                                     >  1:1720215/1‑100 (MQ=255)
                         ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTAAACAACACTTTCCTAGATGCTAAAGCTCGTGATGAAGCCAt                                                                      >  1:1379153/1‑99 (MQ=255)
                          cTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTg                                                                    >  1:1341485/1‑100 (MQ=255)
                           tGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGc                                                                   <  2:2029186/100‑1 (MQ=255)
                            gcgcGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCg                                                                  <  1:2228281/100‑1 (MQ=255)
                            gcgcGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCATCGAAGCTAAAGCTCGTGATGAAGCCATTGCg                                                                  >  1:98270/1‑100 (MQ=255)
                             cgcgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTTGGGATGAAGCCATTGCGt                                                                 <  2:2528163/100‑1 (MQ=255)
                              gcgAAAAGTGCATCCCGTATACCTCTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTa                                                                <  1:2683621/100‑1 (MQ=255)
                              gcgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTGTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTa                                                                <  2:1619251/100‑1 (MQ=255)
                               cgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTAt                                                               >  2:2093577/1‑100 (MQ=255)
                                 ncaaGGGCATCCCGTATACAACTGTTCGTAAAGAAAAAAACTACGGCCAGAGCATCACTTTCCTACATGCACAAGCTAGTGATGAAACTATTGCGTa                                                                >  2:528274/3‑97 (MQ=255)
                                  aaaGGGCATCCCGTATACCACTGTTCGTAAATAAAACAACTACGGCCTGAGCATCACTTTCCACGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTg                                                            <  2:1607547/100‑1 (MQ=255)
                                     gggCATCCCGTATACCACTGTTCGTAAAGAAAACATCTACGGCCTGAGCATCACTGTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGc                                                         <  1:2227738/100‑1 (MQ=255)
                                     gggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTAGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGc                                                         >  1:29466/1‑100 (MQ=255)
                                     gggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGc                                                         <  2:1220645/100‑1 (MQ=255)
                                      ggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAg                                                          <  1:1890007/98‑1 (MQ=255)
                                      ggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCa                                                        >  1:431511/1‑100 (MQ=255)
                                        cATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCGTGAGCATCACTTTCCTAGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAg                                                      >  2:1364443/1‑100 (MQ=255)
                                         aTCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgc                                                     >  2:1309730/1‑100 (MQ=255)
                                            cctgttaCCACTGTTCGTAAAGAAAACTACTACGTCCTTAACATCACTTTACTCGATGCTAAAGCTCTTGATTAAGCCATTGAGTATCTGAGc                                                         <  2:2558212/88‑1 (MQ=255)
                                              gTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGATCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCAt                                                <  1:1031958/100‑1 (MQ=255)
                                                   ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGa                                           >  2:2339544/1‑100 (MQ=255)
                                                   ccACTGTTCGTAAAGAAAACAACTACGGCATGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTAAGCAAGCGCCATCCGGa                                           >  2:1348265/1‑100 (MQ=255)
                                                        gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTgg                                      <  2:843036/100‑1 (MQ=255)
                                                         ttCGTAAAGAAAACAACTAGGGCCTGAGCATCACGTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt                                     <  2:1134376/100‑1 (MQ=255)
                                                         ttCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt                                     >  1:95606/1‑100 (MQ=255)
                                                         tgcGTAAAGAAAACAACTCGGGCCTGGGCATCACTTTCGTCGTTGCTAAAGTTCGTGATGAAGCCATTGCTTATCTGAGCAAGCGCCATCCGGACCTGGt                                     <  2:1182570/98‑1 (MQ=255)
                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  1:517521/100‑1 (MQ=255)
                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  1:2795362/100‑1 (MQ=255)
                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  1:2451113/100‑1 (MQ=255)
                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  1:2168504/100‑1 (MQ=255)
                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  2:633213/100‑1 (MQ=255)
                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  2:15253/100‑1 (MQ=255)
                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCGAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  2:1936610/100‑1 (MQ=255)
                                                             tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTTTCTGAGCAAGCGCCATCTGGACCTGGTGAtt                                 <  1:2043874/100‑1 (MQ=255)
                                                             tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt                                 >  1:1176056/1‑100 (MQ=255)
                                                             tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt                                 <  1:495275/100‑1 (MQ=255)
                                                             tAAAGAAAACAACTACGGCCTGAGCATCACTTTACTCGATGCTAAAGCACGTGATGAAGCCATTTCGTAACTGAACAAGCGCCATCCGGACCTGGTGa                                   >  2:246637/1‑98 (MQ=255)
                                                              aaaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTa                                >  1:2097755/1‑100 (MQ=255)
                                                              aaaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTa                                >  1:716619/1‑100 (MQ=255)
                                                               aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  2:1082125/97‑1 (MQ=255)
                                                               aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                               >  1:1529037/1‑100 (MQ=255)
                                                               aaGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCTTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATGCTGACCTGGTGATTag                               <  2:1416714/100‑1 (MQ=255)
                                                                cgAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagc                              >  2:941326/2‑100 (MQ=255)
                                                                 gAAAACAACTACGGCCTGAGCATCACTTTCCACGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTatca                             >  2:207659/1‑100 (MQ=255)
                                                                  naaaCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGAACCTGGTGATTatcag                            >  2:1320506/2‑100 (MQ=255)
                                                                   aaaCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAACGCCATCCGGACCTGGTGATTagca                             >  1:2829432/1‑98 (MQ=255)
                                                                      caacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTTCGTATCTGAGCAAGCGCCATCCGAATCAGGTGATTAGCAGCCAg                        >  2:2644393/1‑100 (MQ=255)
                                                                       aacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAgg                       <  1:373093/100‑1 (MQ=255)
                                                                       aacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAgg                       <  1:1200961/100‑1 (MQ=255)
                                                                         cTACGGCCTTATCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAgg                       <  1:2612777/98‑1 (MQ=255)
                                                                           aCGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcag                   <  2:386937/100‑1 (MQ=255)
                                                                           aCGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcag                   <  2:2290276/100‑1 (MQ=255)
                                                                           aCGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcag                   <  1:2449714/100‑1 (MQ=255)
                                                                            cGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcag                   <  1:718862/99‑1 (MQ=255)
                                                                            cGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcag                   <  2:1014555/99‑1 (MQ=255)
                                                                             gtccTGAGCATCTCTTTCCTCGATGTTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgca                    <  1:2582219/95‑1 (MQ=255)
                                                                             ggCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcagca                 <  1:1813358/100‑1 (MQ=255)
                                                                              gCCTTAACATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTTCTTATCTTATCAAGCGCCATCCTGACCTAGTTATTAGCAGCCAGGgcag                   <  2:742212/97‑1 (MQ=255)
                                                                                  gAGCATCACTTTCCTCGATGCTAAAGCTCGTTATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAg            <  2:3004283/100‑1 (MQ=255)
                                                                                  gAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAg            >  2:2749657/1‑100 (MQ=255)
                                                                                  gAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAg            <  1:2984722/100‑1 (MQ=255)
                                                                                   atcATCACTTTCCTCGATGTTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGc           <  1:614832/98‑1 (MQ=255)
                                                                                   aGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGc           <  2:457385/100‑1 (MQ=255)
                                                                                    gCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCt          >  1:213064/1‑100 (MQ=255)
                                                                                     cATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtg         <  2:1431144/100‑1 (MQ=255)
                                                                                     cATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtg         >  1:1823180/1‑100 (MQ=255)
                                                                                     cATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCACTGCGTATCTGAGCAAGCGCTATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtg         <  2:1650336/100‑1 (MQ=255)
                                                                                     cATCACTTTCCTCGATACTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCACCATCCGGACATGGTGATTAGCAGCCAGGGCAGCAACCAGc           >  2:2054751/1‑98 (MQ=255)
                                                                                       tCACTTACCTCGATGCTAAAGCTCGGGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGc           <  2:711036/96‑1 (MQ=255)
                                                                                         aCTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtg     >  1:2626737/1‑100 (MQ=255)
                                                                                          cTTTCCTCTATGCTAAAACGCGAGATGAAGCAATTGCGTATCTGAACAAGCGCCATCCGGACTTGGTGCTTAGCAGCCAGGGCAGCAACCAGCtacgtgc    >  2:2777972/1‑100 (MQ=255)
                                                                                            ttCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCgg  >  2:719709/1‑100 (MQ=255)
                                                                                            ttCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCgg  <  1:2495410/100‑1 (MQ=255)
                                                                                                |                                                                                               
AAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGG  >  NC_000913/428092‑428283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑