Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 88,208 A→T 100% T61S (ACA→TCA)  cra → transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091388,2080AT98.8% 363.9 / ‑4.3 118T61S (ACA→TCA) cratranscriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (50/65);  total (51/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATC  >  NC_000913/88112‑88304
                                                                                                |                                                                                                
cGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGctca                                                                                                <  1:1630695/99‑1 (MQ=255)
 gtgtGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACg                                                                                              <  1:2504649/100‑1 (MQ=255)
    taaGCGACAAAACCGTTGAAAAAGTCATAGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTc                                                                                           <  2:2653291/98‑1 (MQ=255)
     gAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCt                                                                                          >  1:1037971/1‑100 (MQ=255)
      aGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTa                                                                                         >  1:1816798/1‑100 (MQ=255)
      aGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTa                                                                                         >  1:1769732/1‑100 (MQ=255)
       gCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTAt                                                                                        <  1:2726138/100‑1 (MQ=255)
       gCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTAGCAGAAGGGCATCGTGCTGGACGCTCACGTTc                                                                                           >  1:898769/1‑97 (MQ=255)
        cGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTTGGCTTCGTGCTGGACGCTCACGTTCTAtt                                                                                       <  2:2432120/100‑1 (MQ=255)
         gACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTAGCAACTGGGCTTCGTGCTGGACGCACACGTTCTATTg                                                                                      >  1:2753156/1‑100 (MQ=255)
           cAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGt                                                                                    >  2:735624/1‑100 (MQ=255)
             aaaCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTc                                                                                   >  2:427592/1‑99 (MQ=255)
             aaaCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCt                                                                                  <  2:1968547/100‑1 (MQ=255)
               aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtg                                                                                <  1:357993/100‑1 (MQ=255)
               aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtg                                                                                <  1:1892110/100‑1 (MQ=255)
               aCCGTTGAAAAAGTCATGGCTGTGGCGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGCCGCTCACGTTCTATTGGTCttn                                                                                <  2:2255651/100‑2 (MQ=255)
                ccGTTGAAAACGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCATCGTGCTGGACGCTCACGTTCTATTGGTCTtgt                                                                               <  2:1998113/100‑1 (MQ=255)
                ccGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCTCCCGAATGCGGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgt                                                                               <  1:140038/100‑1 (MQ=255)
                ccGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgt                                                                               <  2:2376934/100‑1 (MQ=255)
                ccGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgt                                                                               <  1:2951164/100‑1 (MQ=255)
                 cGTTGAAAAAGTCATGGCTGTGGTGCGTGAGTATAATTACCACTCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgtg                                                                              <  1:129419/100‑1 (MQ=255)
                 cGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgtg                                                                              >  2:2612553/1‑100 (MQ=255)
                  gTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgtg                                                                              <  1:1150056/99‑1 (MQ=255)
                    tGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATc                                                                           >  1:1761798/1‑100 (MQ=255)
                      aaaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACTCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATccc                                                                         <  1:1915598/100‑1 (MQ=255)
                      aaaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATccc                                                                         <  1:663959/100‑1 (MQ=255)
                      aaaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATccc                                                                         <  1:355624/100‑1 (MQ=255)
                        aaaGTCATGGCTTTGGTGCGTGAGCACAATTACCATCCGAACGCCGTGGCAGCTGGGCTTTGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATcccc                                                                        <  1:1076601/99‑1 (MQ=255)
                        aaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCg                                                                       <  1:2687526/100‑1 (MQ=255)
                         aaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGa                                                                      <  1:2612585/100‑1 (MQ=255)
                           gTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATc                                                                    >  1:1744152/1‑100 (MQ=255)
                            tCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCt                                                                   <  2:1873918/100‑1 (MQ=255)
                             cATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCt                                                                   >  1:2177743/1‑99 (MQ=255)
                             cATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTg                                                                  >  2:2875153/1‑100 (MQ=255)
                             cATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTg                                                                  >  1:2596377/1‑100 (MQ=255)
                              aTGGCTGTGGTGCGTTAGCTCAATTACCACCCGAACGCCGTGGCAGCTGTGCGTCGTGCTGGACGCGCACTTTCTATTTGTCTTGTGATCCCCGATCTgg                                                                 <  2:2560062/100‑1 (MQ=255)
                              aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTgg                                                                 <  2:1895662/100‑1 (MQ=255)
                              aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTgg                                                                 <  2:2436135/100‑1 (MQ=255)
                               tGGCTGTGGTGCGTGTGCACAATTACTTCCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGga                                                                <  1:2745727/100‑1 (MQ=255)
                               tGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTgg                                                                 >  2:167396/1‑99 (MQ=255)
                               tGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGga                                                                <  1:2942953/100‑1 (MQ=255)
                                ggCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGgag                                                               <  2:2488496/100‑1 (MQ=255)
                                 gttgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGgag                                                               <  1:1405130/97‑1 (MQ=255)
                                 gCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGgaga                                                              <  1:1445628/100‑1 (MQ=255)
                                 gCTGTGGAGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATATGgaga                                                              >  2:776998/1‑100 (MQ=255)
                                  cTGTGGTGGGTGGGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGgag                                                               <  2:2488532/98‑1 (MQ=255)
                                  cTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGTCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTgg                                                                 <  2:2126796/96‑1 (MQ=255)
                                   tgtgGTGCGTGAGCACAATTACCACCCGATCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAac                                                            <  1:1265798/100‑1 (MQ=255)
                                    gtggtgCGTCAGCACAATTACCACCCCAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACCTTATATTGGTCTTGTGATCACCGATCTGGAGAaca                                                           <  2:2422020/100‑1 (MQ=255)
                                       gtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCa                                                        >  2:323862/1‑100 (MQ=255)
                                       gtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCa                                                        >  2:2883299/1‑100 (MQ=255)
                                       gtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTATGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACAcc                                                         <  1:1220740/99‑1 (MQ=255)
                                        tgCGTGAGCATAATAAGTACCCGATCGACTTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATTTCTGATCTGGAGAACACCa                                                        <  2:2878343/99‑1 (MQ=255)
                                        tgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACTAg                                                       <  2:198340/100‑1 (MQ=255)
                                         gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTTTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGc                                                      <  2:1614857/100‑1 (MQ=255)
                                         gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGc                                                      >  1:2455300/1‑100 (MQ=255)
                                          cGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGAACTATTAGTCTTGTGATCCCCGATCTGGAGAACACCAGCt                                                     >  1:302035/1‑100 (MQ=255)
                                            tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCtat                                                   <  1:1544549/100‑1 (MQ=255)
                                              aGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATAc                                                 <  1:3018569/100‑1 (MQ=255)
                                              aGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGAATTGTGATCCCCGATCTGGAGAACACCAGCTATAc                                                 >  1:1646272/1‑100 (MQ=255)
                                               gCACAATTACCAGCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTTGTCTTGTGATCCTCGATCTGGAGAACACCAGCtata                                                  <  2:1094306/98‑1 (MQ=255)
                                               gCACAATTACCACCCGAACGCCGTGGTAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATAcc                                                >  1:878152/1‑100 (MQ=255)
                                                cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATAccc                                               >  2:1412787/1‑100 (MQ=255)
                                                cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATAccc                                               >  2:1732622/1‑100 (MQ=255)
                                                 acaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCg                                              <  1:2614942/100‑1 (MQ=255)
                                                  caATTACCACCCGAACGCCGTGGCCGCTGGGCTTCGTTCTGGACGCTCACGTTCTATTAGTCTTTTAATACAAGATCTGGAGAACACCAGCTATACCCGc                                             >  1:936900/1‑100 (MQ=255)
                                                    aTTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCAt                                           >  2:1354406/1‑100 (MQ=255)
                                                     ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATc                                          <  2:2953018/100‑1 (MQ=255)
                                                     ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATc                                          <  1:1945088/100‑1 (MQ=255)
                                                      tACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCg                                         <  1:323865/100‑1 (MQ=255)
                                                      tACCACCCGAACGCCGTGGCAGCTGGGCTCCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCACCGATCTGGAGAACACCAGCTATACCCGCATCg                                         <  2:1816777/100‑1 (MQ=255)
                                                      tACCACCCGAACGCAGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATATGGAGAACACCAGCTATACACGctt                                           >  2:2948851/1‑96 (MQ=255)
                                                       accaccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGc                                        >  1:2471828/1‑100 (MQ=255)
                                                       accaccCGAACGCCGTGGCAGCTGGGCATCGTGCTGGCCGCTCACGTGCTATTGGTCTTGTGATCCCCTATCTGGAGCACACCAGCTATACCCGCATCGc                                        >  2:2407149/1‑100 (MQ=255)
                                                        ccaccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCt                                       <  2:556651/100‑1 (MQ=255)
                                                        ccaccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCt                                       <  2:533460/100‑1 (MQ=255)
                                                        ccaccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCt                                       >  2:1755098/1‑100 (MQ=255)
                                                         caccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                      <  1:23150/100‑1 (MQ=255)
                                                          accCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                      <  2:778692/99‑1 (MQ=255)
                                                            ccGAACGTCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAAcn                                   <  2:1622204/100‑2 (MQ=255)
                                                             cGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTa                                  <  2:1291132/100‑1 (MQ=255)
                                                               aaCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTAtn                                <  2:840304/100‑2 (MQ=255)
                                                                 cGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGTTCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCtt                              >  2:1145188/1‑100 (MQ=255)
                                                                     gTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                          >  1:1647272/1‑100 (MQ=255)
                                                                       ggCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGc                        >  2:1735087/1‑100 (MQ=255)
                                                                        gCAGCTGGGCTTCGTGTTTGACGCTCACGTTTTATTTGTTTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGcc                       <  1:729378/100‑1 (MQ=255)
                                                                        gCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGcc                       <  2:652252/100‑1 (MQ=255)
                                                                        gCAGCTGGGCTTCGTGCCGGACGCTCACGTTCTATTGGTCTTGTGATCCCTGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGcc                       >  1:2796523/1‑100 (MQ=255)
                                                                           gCTGGGCTTCTTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAgg                    <  1:260358/100‑1 (MQ=255)
                                                                             tGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGgcg                  >  2:14434/1‑100 (MQ=255)
                                                                                gCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCgg               >  1:648337/1‑100 (MQ=255)
                                                                                 cTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGCTCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGc              <  2:1941293/100‑1 (MQ=255)
                                                                                     gTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACg          >  1:972936/1‑100 (MQ=255)
                                                                                      tGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACgg         <  2:1803862/100‑1 (MQ=255)
                                                                                        cTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACgggg       <  1:143203/100‑1 (MQ=255)
                                                                                         tGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGt      >  2:46698/1‑100 (MQ=255)
                                                                                          ggACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGtt     >  2:2681702/1‑100 (MQ=255)
                                                                                           gACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTa    <  1:2713344/100‑1 (MQ=255)
                                                                                             cGCTCACGTTCTATTGGTCTTGTGTTTTGTGATTTGTAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATc  <  1:2582981/100‑1 (MQ=255)
                                                                                             cGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATc  >  1:1178505/1‑100 (MQ=255)
                                                                                                |                                                                                                
CGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATC  >  NC_000913/88112‑88304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: