BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	662,636	T→G	100%	T3P (ACC→CCC)	ybeD ←	UPF0250 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,636	0	T	G	98.8%	360.5 / ‑4.2	107	T3P (ACC→CCC)	ybeD	UPF0250 family protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (43/62); total (44/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTAC  >  NC_000913/662539‑662732

aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGgtt                                                                                                >  1:2689762/1‑100 (MQ=255)

aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGgtt                                                                                                >  1:1691248/1‑100 (MQ=255)

aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGgtt                                                                                                <  2:613908/100‑1 (MQ=255)

 ccACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGgttt                                                                                               >  2:2534633/1‑100 (MQ=255)

   aCTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCa                                                                                             <  1:195925/100‑1 (MQ=255)

    cTTCAACCACCTGATCAACCAGCTCAGGTAACGTCTGCTCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCa                                                                                             <  1:2432161/99‑1 (MQ=255)

    cTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCAt                                                                                            <  1:583709/100‑1 (MQ=255)

    cTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCAt                                                                                            <  1:2505570/100‑1 (MQ=255)

     ttCAGCCACCTGATCAACCCGCTAAGTTAACGCCTGCCCCATAACTTTGTAAGTAAAATGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTc                                                                                              <  2:1430978/97‑1 (MQ=255)

     ttCAACCACCTGATCAACCAGCTCAGGTAACGTCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATg                                                                                           <  1:217689/100‑1 (MQ=255)

     ttCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATg                                                                                           <  1:1102571/100‑1 (MQ=255)

         accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAg                                                                                       <  2:2349997/100‑1 (MQ=255)

         accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAg                                                                                       >  1:1860519/1‑100 (MQ=255)

          ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGc                                                                                      >  2:2565174/1‑100 (MQ=255)

           caccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAACAGGAGTAGGGAATTCAAGCCGTTCGTTAAGTTTGGGTTTCATGTCAGCt                                                                                     <  2:1766189/100‑1 (MQ=255)

             ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAATCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTcc                                                                                   <  2:2007770/100‑1 (MQ=255)

             ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTcc                                                                                   <  2:2250806/100‑1 (MQ=255)

             ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTcc                                                                                   >  2:1226371/1‑100 (MQ=255)

              cTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCg                                                                                  >  1:650284/1‑100 (MQ=255)

               tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCgg                                                                                 <  1:1757521/100‑1 (MQ=255)

               tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCgg                                                                                 >  2:2547928/1‑100 (MQ=255)

                 aTCAACCAGCTTAAGTAAAGTCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcg                                                                               <  1:2600019/100‑1 (MQ=255)

                 aTCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcg                                                                               <  2:2217742/100‑1 (MQ=255)

                 aTCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcg                                                                               <  1:2812578/100‑1 (MQ=255)

                     acaaGCTCAGGTAACGCCTGCCCCATAACTATGTAAGTAAAAGGAGTAGGGAATTCAAGAAGTTCGTTAAGTTTGGTTTTCATGTCACCTCCGGcgtaac                                                                           >  2:615723/4‑100 (MQ=255)

                     aCCAGCTCAGGTAACGCCTGCCCCATATCTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTTGGTTTCATGTCAGCTCCGGcgtaa                                                                            <  2:2230996/99‑1 (MQ=255)

                     aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaac                                                                           >  2:1130545/1‑100 (MQ=255)

                     aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaac                                                                           >  2:705672/1‑100 (MQ=255)

                      ccAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacg                                                                          <  2:264103/100‑1 (MQ=255)

                       cAGCTCAGGTAACGCCTGCCCCATAACTTTTTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacgt                                                                         <  1:1213145/100‑1 (MQ=255)

                       cAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacgt                                                                         >  2:2980413/1‑100 (MQ=255)

                       cAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacgt                                                                         <  1:1861202/100‑1 (MQ=255)

                        aGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacg                                                                          <  1:1405106/98‑1 (MQ=255)

                         gCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacgtaa                                                                       <  2:222864/100‑1 (MQ=255)

                              ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTaaa                                                                  >  2:2790911/1‑100 (MQ=255)

                               gTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTTAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAAt                                                                 <  1:883055/100‑1 (MQ=255)

                                tAACGTCTGCCCCATAACTTTGTACGTAAAAGGATTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGTGTAACGTAAGTAAAt                                                                 <  2:1488024/99‑1 (MQ=255)

                                tAACGCCTGTCCCATAACTTTGTAAGTAAAATGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATa                                                                <  1:2742171/100‑1 (MQ=255)

                                tAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATa                                                                <  1:1800515/100‑1 (MQ=255)

                                 aaCGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAg                                                               <  1:12809/100‑1 (MQ=255)

                                 aaCGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAg                                                               <  2:1691228/100‑1 (MQ=255)

                                     ccTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAAc                                                           >  1:2016348/1‑100 (MQ=255)

                                     ccTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAAc                                                           >  1:851512/1‑100 (MQ=255)

                                      cTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACt                                                          <  1:906548/100‑1 (MQ=255)

                                      cTGCCCCATAACTGTGTAAGTAAAAGGAGTAGGGAATTCAAACAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACt                                                          >  2:2257494/1‑100 (MQ=255)

                                        gCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTcc                                                        >  1:574477/1‑100 (MQ=255)

                                         ccccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGAAATTAAATAGCAACTccc                                                       >  1:2108941/1‑100 (MQ=255)

                                          cccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCg                                                      >  2:1846561/1‑100 (MQ=255)

                                          cccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCg                                                      >  1:1241594/1‑100 (MQ=255)

                                           ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGc                                                     >  1:2991891/1‑100 (MQ=255)

                                            cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCg                                                      >  1:1267436/1‑98 (MQ=255)

                                            cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGcc                                                    <  2:851508/100‑1 (MQ=255)

                                            cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGcc                                                    <  2:695493/100‑1 (MQ=255)

                                              tAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGcn                                                    <  1:581232/98‑2 (MQ=255)

                                               aaCGTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAg                                                  <  2:1860498/99‑1 (MQ=255)

                                                 cTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAg                                               >  2:2834500/1‑100 (MQ=255)

                                                  tttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAgg                                              >  1:2313621/1‑100 (MQ=255)

                                                    tGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTACGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCg                                            >  1:1702162/1‑100 (MQ=255)

                                                      taagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggg                                          <  1:2843499/100‑1 (MQ=255)

                                                       aagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGa                                         <  1:2286888/100‑1 (MQ=255)

                                                       aagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGa                                         <  1:801343/100‑1 (MQ=255)

                                                        agtaaAAGGCTTAGGAAATTCAAGCAGATAGTTAAGATGGGGATTCATGACACAACCGGCGTAACGTAATCGAATAGCAACTCCCGCCAGAAGGCg                                            >  2:2840519/1‑96 (MQ=255)

                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                        >  2:1399216/1‑100 (MQ=255)

                                                         gtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAGt                                       <  2:1011013/100‑1 (MQ=255)

                                                           aaaaCGAGTAAGGAATTCAATCAGTTCTGTTAGTTTGCGTTTCATGTCAGCTCCGGCGCAACGTAATTAAATTGCAACTCCCTCCAGAAGGCGGGAGt                                       <  2:1956435/98‑1 (MQ=255)

                                                             aaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                   <  2:574474/100‑1 (MQ=255)

                                                              aGGAGTAGGGAATTTAAGCATTTTTTTTAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                   <  1:707831/99‑1 (MQ=255)

                                                              aGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttttt                                  <  1:2565205/100‑1 (MQ=255)

                                                              aGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttttt                                  <  1:3007569/100‑1 (MQ=255)

                                                                  gTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTg                              <  1:2862968/100‑1 (MQ=255)

                                                                   tAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGa                             <  1:2898483/100‑1 (MQ=255)

                                                                       taaTTTAAGCAGTTCGTTAAGTTTGTGTTTCATGTAAGCTTCGGGGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATg                           <  1:1223118/97‑1 (MQ=255)

                                                                       gAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCt                         >  1:454932/1‑100 (MQ=255)

                                                                         aTTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCTCCAGAAGGCGGGAGTTTTTTACTGATGCTTa                       <  2:1166712/100‑1 (MQ=255)

                                                                           tCAAGCAGTTCGTTATGTTTGGGTTTCATGTTATCTCCGTCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGt                     <  1:569719/100‑1 (MQ=255)

                                                                            cAAGCATTTCGTTAAGTTTGGGTTTCATGTCATCTCCGGCGTAACGTAATTAAATATCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                    <  2:41994/100‑1 (MQ=255)

                                                                            cAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                    <  1:1130556/100‑1 (MQ=255)

                                                                            cAAGCAGTTCGTTAAGTTTGAGTTTCATGTCAGCTCCAGCGTAACGTAATTAAATAGCAACTCTCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                    <  2:2996509/100‑1 (MQ=255)

                                                                              aGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGtata                  >  1:2595971/1‑100 (MQ=255)

                                                                               gCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGtata                  <  1:162988/99‑1 (MQ=255)

                                                                               gCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTatat                 >  1:2417523/1‑100 (MQ=255)

                                                                                cAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATg                <  1:1226383/100‑1 (MQ=255)

                                                                                 aggtCGTTAAGTGTGGGTTTCATGTTAGCTACGGCGTAATGTACTTAAATAGCAACTCCCTCAAGAAGGCGGGAGGTTATAACTGATGCTTAGTATGTgg               >  2:2283599/4‑100 (MQ=255)

                                                                                 aGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgg               >  1:1722789/1‑100 (MQ=255)

                                                                                  gTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg              <  1:1506575/100‑1 (MQ=255)

                                                                                  gTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg              <  2:144266/100‑1 (MQ=255)

                                                                                  gTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg              <  2:144289/100‑1 (MQ=255)

                                                                                  gTTCGTTAAGTTTGGGTATCATATCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg              >  2:1772714/1‑100 (MQ=255)

                                                                                  gTTCGTTAAGTTTCGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTGACTGATGCTTAGTATATggg              <  2:1040039/100‑1 (MQ=255)

                                                                                     cGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAc           >  2:1647740/1‑100 (MQ=255)

                                                                                     cGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAc           >  2:1386452/1‑100 (MQ=255)

                                                                                     cGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAc           >  2:1898393/1‑100 (MQ=255)

                                                                                      gTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACg          >  2:1141220/1‑100 (MQ=255)

                                                                                      gTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAAGCGGGAGTATTTTACTGATGCTTAGTATATGGGGACg          >  1:376210/1‑100 (MQ=255)

                                                                                           gTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGaaa      >  1:3010875/1‑99 (MQ=255)

                                                                                           gTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAt     >  2:2883509/1‑100 (MQ=255)

                                                                                           gTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAt     <  2:2320749/100‑1 (MQ=255)

                                                                                            tttGGTTTTCATGTCGGCTCCGGCGTTACGTAATTAAATATCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAtt    <  1:921809/100‑1 (MQ=255)

                                                                                              tGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTac  <  1:1915891/100‑1 (MQ=255)

                                                                                              tGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGAAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTac  <  2:2539447/100‑1 (MQ=255)

ACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTAC  >  NC_000913/662539‑662732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`