Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,699 T→A 18.4% intergenic (+36/+24) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,6990TA18.4% 273.9 / 51.7 118intergenic (+36/+24)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base T (37/59);  new base A (12/10);  total (49/69)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.49e-02

Only 100 of 122 total aligned reads displayed.

AATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACT  >  NC_000913/681600‑681796
                                                                                                   |                                                                                                 
     gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGtt                                                                                                   >  2:143629/1‑95 (MQ=255)
     gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgc                                                                                              >  1:1062831/1‑100 (MQ=255)
     gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCATTTAcgc                                                                                              >  1:1496771/1‑100 (MQ=255)
      tttAACGGTGCTGGTGATTGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACTATGTGTTTTTTGCGCCGATGCTGGATGCAGCGTTTAcgcc                                                                                             <  2:198158/100‑1 (MQ=255)
      tttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgcc                                                                                             >  1:2264943/1‑100 (MQ=255)
      tttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgcc                                                                                             >  1:2264171/1‑100 (MQ=255)
          aCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCAt                                                                                         >  1:282610/1‑100 (MQ=255)
            ggTGCTTGTGATAGGTTATTCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACTATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATcc                                                                                       <  1:2488153/100‑1 (MQ=255)
              tGCTGGTGATAGGTTATGCTCTCTTCAAAATCGCTATGTTGTTTAGCTGACTATGTGTTTTTTGCGATGATGCCGTTTGTTGCTTTTACGCCGCATCCgg                                                                                     <  2:1424798/100‑1 (MQ=255)
              tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGTGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                     <  2:2235840/100‑1 (MQ=255)
              tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  1:1812307/100‑1 (MQ=38)
              tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  1:1841106/100‑1 (MQ=38)
              tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  2:58948/100‑1 (MQ=38)
              tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  2:240127/100‑1 (MQ=38)
              tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  2:1768826/100‑1 (MQ=38)
              tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                     <  1:1980990/100‑1 (MQ=255)
              tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                      <  2:1262349/99‑1 (MQ=255)
               gCTGGTGATAGGTTATGCTCTCGGCAAATTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                    <  2:766804/100‑1 (MQ=255)
               gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                    <  1:966044/100‑1 (MQ=255)
               gCTGGTGATAGGTGATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGTATGCGGCGTAAACTCTGCATCCgg                                                                                     <  2:466901/99‑1 (MQ=255)
                cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                   <  2:444057/100‑1 (MQ=255)
                cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                   <  2:1040266/100‑1 (MQ=255)
                 tGGTGATAGGTTATGCTCTCGGCAAAATCGCGCTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCat                                                                                  <  2:2132814/100‑2 (MQ=37)
                 tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                    >  2:2142189/1‑98 (MQ=255)
                   gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCTATGCCGTATGCAGCGTTTACGCAGCATCCGGCATcc                                                                                >  1:536845/1‑100 (MQ=255)
                   gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                >  1:1219539/1‑100 (MQ=255)
                   gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                <  1:391548/100‑1 (MQ=255)
                        aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACTCCGCATCCGGCAGCCGTGtt                                                                           <  2:1182152/100‑1 (MQ=255)
                        aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                           <  1:669082/100‑1 (MQ=255)
                         ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGttt                                                                          <  1:1577821/100‑1 (MQ=255)
                          gTTAGGCTCTCGGCAAAATCGCGATTTTGTTTAGCTTTCGATGTGTGTTTGGCGCCTATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTa                                                                         <  1:1765048/100‑1 (MQ=38)
                            tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                       <  2:2182723/100‑1 (MQ=255)
                              tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                     <  1:2172997/100‑1 (MQ=255)
                              tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                     <  1:835727/100‑1 (MQ=255)
                              tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                     <  2:1355921/100‑1 (MQ=255)
                                ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                   >  1:436308/1‑100 (MQ=255)
                                ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                   >  1:2913601/1‑100 (MQ=255)
                                ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                   >  1:1919778/1‑100 (MQ=255)
                                ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCAGTGTTTATGGGa                                                                    >  1:1337053/1‑99 (MQ=255)
                                 tctcGGCAAAATCGCGATGTTGTTTAGCTGACGTTGTGTTTTTTGTGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtc                                                                  <  1:1194825/100‑1 (MQ=255)
                                  ctcGGCAAAATCGCGATGTTGTTTAGCTGAGTATGTGTTTTTTGCGCTGATGCCTGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtct                                                                 <  2:563553/100‑1 (MQ=39)
                                    cGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCa                                                               >  2:726633/1‑100 (MQ=255)
                                      gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                             >  1:2302919/1‑100 (MQ=255)
                                       cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                            <  1:910773/100‑1 (MQ=255)
                                       cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGACAGCCGTGTTTATGGGATCTCAAt                                                             >  1:2819836/1‑99 (MQ=255)
                                        aaaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                           >  2:57055/1‑100 (MQ=255)
                                        aaaaTCGCGAGGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGAGGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                           <  2:2811571/100‑1 (MQ=255)
                                           aTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTa                                                        <  2:1452034/100‑1 (MQ=255)
                                            tcgttaTGTTTTTTATTTGACTATTTGTTTTTTGCGCCGAGTCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGc                                                          <  1:1341139/92‑1 (MQ=37)
                                               cgATGGTGTTTCGGTGACGATGTGTTTTTTGCGCCGATGGCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                     <  2:625774/99‑1 (MQ=39)
                                                 aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                                  <  1:1905551/100‑1 (MQ=255)
                                                  tgttgtTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGTGATCTCAATGGCTAAATAAtc                                                 <  1:1265807/100‑1 (MQ=255)
                                                  tgttgtTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtc                                                 <  1:2506353/100‑1 (MQ=255)
                                                   gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                <  1:692760/100‑1 (MQ=255)
                                                    ttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgt                                               <  2:1358167/100‑2 (MQ=255)
                                                      gttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctc                                             >  1:1227829/1‑100 (MQ=255)
                                                       tttAGCTGGCGATGTCTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCTGTGTTTATGGGATCTGTATGGCTAAATAAtcg                                                <  2:2389790/96‑1 (MQ=38)
                                                         tAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcc                                          <  1:1594827/100‑1 (MQ=255)
                                                           gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                        >  2:407455/1‑100 (MQ=255)
                                                           gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                        >  1:430496/1‑100 (MQ=255)
                                                             tGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                      >  1:1084891/1‑100 (MQ=255)
                                                             tGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                      >  1:269591/1‑100 (MQ=255)
                                                              gACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                      >  1:219226/1‑99 (MQ=255)
                                                                cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                   >  1:799391/1‑100 (MQ=255)
                                                                cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGAATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                   >  1:1001521/1‑100 (MQ=255)
                                                                 gATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgccg                                  >  2:1052042/1‑100 (MQ=255)
                                                                 gATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgccg                                  <  2:879141/100‑1 (MQ=255)
                                                                  aTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGa                                 <  1:1100202/100‑1 (MQ=255)
                                                                   tgtgTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGaa                                >  1:944038/1‑100 (MQ=255)
                                                                   tgtgTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGaa                                >  1:1290640/1‑100 (MQ=255)
                                                                    gtgtTTGTTGGGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAAc                               <  2:2853121/100‑1 (MQ=255)
                                                                      gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                             >  1:958609/1‑100 (MQ=255)
                                                                      gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                             <  1:1836726/100‑1 (MQ=255)
                                                                         ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                          <  1:634829/100‑1 (MQ=255)
                                                                          tttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                         <  2:1217638/100‑1 (MQ=255)
                                                                              cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTCTGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAc                      >  2:445747/1‑99 (MQ=255)
                                                                              cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                     >  2:758354/1‑100 (MQ=255)
                                                                                  gctGCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGACTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                 >  1:382132/3‑100 (MQ=37)
                                                                                  gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                 <  1:1704667/100‑1 (MQ=255)
                                                                                  gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCgg                  <  1:2170182/99‑1 (MQ=255)
                                                                                  gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGAATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                 >  1:2829638/1‑100 (MQ=255)
                                                                                     gCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa              >  2:2661359/1‑100 (MQ=38)
                                                                                     gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa              <  2:2962190/100‑1 (MQ=255)
                                                                                      ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat             >  1:2670222/1‑100 (MQ=38)
                                                                                      ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCACTCAACCGCAGCCGAACTTCATCCACGCGGGCCat             >  1:857975/1‑100 (MQ=25)
                                                                                       cGGATGCGGCGTAAACGCTGCATCCAGCAGCCGTGTTTATGGGATCGCAATGGCTAAATCATCGCTCGCCCGCCGCAGAACTTCATCCa                       >  2:1580197/1‑89 (MQ=25)
                                                                                        ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat           >  1:2448999/1‑100 (MQ=38)
                                                                                        ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat           >  2:1600543/1‑100 (MQ=38)
                                                                                        ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat           >  1:1135278/1‑100 (MQ=38)
                                                                                        ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat           >  1:870044/1‑100 (MQ=38)
                                                                                              aGCGTTTACGCCGCATCCGGCAGTCGTGTTTATGGGATTTTAATGGCTAAATAATCGCTCGCGCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  1:1337250/100‑1 (MQ=39)
                                                                                              aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  2:944032/100‑1 (MQ=255)
                                                                                              aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  1:438793/100‑1 (MQ=255)
                                                                                              aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  2:2264146/100‑1 (MQ=255)
                                                                                              aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  2:2264918/100‑1 (MQ=255)
                                                                                                catttACGCCGTATCTGGCAGCCGTGTTTATGGTATCTCAATGGCTAAATAATCGCTCTCCCGCCGCCGAACTTCATCCACGTGGGCCATATCGTTTGAc   <  2:2882240/98‑1 (MQ=255)
                                                                                                cGTTTACGCCGCATCCGGCAGCGGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc   >  2:1760085/1‑100 (MQ=255)
                                                                                                cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc   <  1:1672739/100‑1 (MQ=255)
                                                                                                 gTTTACGCCGCATCCGGCAGCTGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACt  <  1:1361587/100‑1 (MQ=255)
                                                                                                 ctttACGCCGCATCCAGCAGCCGAGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTACATCCACGCGGGCCATATCGTTTGACt  >  2:1228827/2‑100 (MQ=255)
                                                                                                   |                                                                                                 
AATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACT  >  NC_000913/681600‑681796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: