Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,700 T→A 17.7% intergenic (+37/+23) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,7000TA17.7% 270.9 / 49.7 118intergenic (+37/+23)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base T (38/59);  new base A (11/10);  total (49/69)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.10e-01

Only 100 of 120 total aligned reads displayed.

AGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACT  >  NC_000913/681604‑681796
                                                                                                |                                                                                                
aGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcg                                                                                               >  2:1107210/1‑100 (MQ=255)
 gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgc                                                                                              >  1:1062831/1‑100 (MQ=255)
  tttAACGGTGCTGGTGATTGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACTATGTGTTTTTTGCGCCGATGCTGGATGCAGCGTTTAcgcc                                                                                             <  2:198158/100‑1 (MQ=255)
  tttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgcc                                                                                             >  1:2264943/1‑100 (MQ=255)
   ttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccg                                                                                            <  1:2727721/100‑1 (MQ=255)
    tAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccgc                                                                                           >  2:734594/1‑100 (MQ=255)
      aCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCAt                                                                                         >  1:282610/1‑100 (MQ=255)
        ggTGCTTGTGATAGGTTATTCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACTATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATcc                                                                                       <  1:2488153/100‑1 (MQ=255)
          tGCTGGTGATAGGTTATGCTCTCTTCAAAATCGCTATGTTGTTTAGCTGACTATGTGTTTTTTGCGATGATGCCGTTTGTTGCTTTTACGCCGCATCCgg                                                                                     <  2:1424798/100‑1 (MQ=255)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGTGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                     <  2:2235840/100‑1 (MQ=255)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  2:1768826/100‑1 (MQ=38)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  2:240127/100‑1 (MQ=38)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  2:58948/100‑1 (MQ=38)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  1:1841106/100‑1 (MQ=38)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                     <  1:1812307/100‑1 (MQ=38)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                     <  1:1980990/100‑1 (MQ=255)
          tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                      <  2:1262349/99‑1 (MQ=255)
           gCTGGTGATAGGTTATGCTCTCGGCAAATTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                    <  2:766804/100‑1 (MQ=255)
           gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGc                                                                                    <  1:78333/100‑1 (MQ=38)
           gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                    <  1:966044/100‑1 (MQ=255)
            cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCa                                                                                   <  1:1890559/100‑1 (MQ=38)
            cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                   <  2:1040266/100‑1 (MQ=255)
            cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                   <  2:444057/100‑1 (MQ=255)
             tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                    >  2:2142189/1‑98 (MQ=255)
               gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCTATGCCGTATGCAGCGTTTACGCAGCATCCGGCATcc                                                                                >  1:536845/1‑100 (MQ=255)
               gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                >  1:1219539/1‑100 (MQ=255)
               gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                <  1:391548/100‑1 (MQ=255)
                tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCg                                                                               >  1:3032263/1‑100 (MQ=255)
                 gATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgt                                                                              >  2:1905528/1‑100 (MQ=255)
                    aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACTCCGCATCCGGCAGCCGTGtt                                                                           <  2:1182152/100‑1 (MQ=255)
                    aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                           <  1:669082/100‑1 (MQ=255)
                     ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGttt                                                                          <  1:1577821/100‑1 (MQ=255)
                        tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                       <  1:1709176/100‑1 (MQ=255)
                        tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                       <  2:2182723/100‑1 (MQ=255)
                          tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                     <  1:2172997/100‑1 (MQ=255)
                          tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                     <  1:835727/100‑1 (MQ=255)
                          tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                     <  2:1355921/100‑1 (MQ=255)
                           gCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGa                                                                    <  2:148919/100‑1 (MQ=255)
                            ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                   >  1:2913601/1‑100 (MQ=255)
                            ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCAGTGTTTATGGGa                                                                    >  1:1337053/1‑99 (MQ=255)
                             tctcGGCAAAATCGCGATGTTGTTTAGCTGACGTTGTGTTTTTTGTGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtc                                                                  <  1:1194825/100‑1 (MQ=255)
                              ctcGGCAAAATCGCGATGTTGTTTAGCTGAGTATGTGTTTTTTGCGCTGATGCCTGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtct                                                                 <  2:563553/100‑1 (MQ=39)
                               tcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtc                                                                  <  1:2180998/98‑1 (MQ=255)
                                  gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                             >  1:2302919/1‑100 (MQ=255)
                                   cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGACAGCCGTGTTTATGGGATCTCAAt                                                             >  1:2819836/1‑99 (MQ=255)
                                    aaaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                           >  2:57055/1‑100 (MQ=255)
                                    aaaaTCGCGAGGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGAGGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                           <  2:2811571/100‑1 (MQ=255)
                                       aTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTa                                                        <  2:1452034/100‑1 (MQ=255)
                                        tcgttaTGTTTTTTATTTGACTATTTGTTTTTTGCGCCGAGTCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGc                                                          <  1:1341139/92‑1 (MQ=37)
                                        tCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTATACGCAGCATCCGGCAGCCGTGTTTATTGAAACTCAATGGCTa                                                        >  1:502954/1‑99 (MQ=255)
                                           cgATGGTGTTTCGGTGACGATGTGTTTTTTGCGCCGATGGCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                     <  2:625774/99‑1 (MQ=39)
                                             aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                                  <  1:1905551/100‑1 (MQ=255)
                                             aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACACCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                                  >  1:2421653/1‑100 (MQ=255)
                                              tgttgtTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGTGATCTCAATGGCTAAATAAtc                                                 <  1:1265807/100‑1 (MQ=255)
                                                ttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgt                                               <  2:1358167/100‑2 (MQ=255)
                                                  gttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctc                                             >  1:1227829/1‑100 (MQ=255)
                                                   tttAGCTGGCGATGTCTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCTGTGTTTATGGGATCTGTATGGCTAAATAAtcg                                                <  2:2389790/96‑1 (MQ=38)
                                                    ttAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctcgc                                           <  2:2512453/100‑1 (MQ=255)
                                                     tAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcc                                          <  1:1594827/100‑1 (MQ=255)
                                                       gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                        >  2:407455/1‑100 (MQ=255)
                                                       gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                        >  1:430496/1‑100 (MQ=255)
                                                         tGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                      >  1:1084891/1‑100 (MQ=255)
                                                         tGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                      >  1:269591/1‑100 (MQ=255)
                                                          gACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                      >  1:219226/1‑99 (MQ=255)
                                                            cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                   >  1:799391/1‑100 (MQ=255)
                                                            cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGAATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                   >  1:1001521/1‑100 (MQ=255)
                                                             gATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgccg                                  <  2:879141/100‑1 (MQ=255)
                                                              aTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGa                                 <  1:1100202/100‑1 (MQ=255)
                                                               tgtgTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGaa                                >  1:1290640/1‑100 (MQ=255)
                                                               tgtgTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGaa                                >  1:944038/1‑100 (MQ=255)
                                                                gtgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAAc                               <  2:510252/100‑1 (MQ=255)
                                                                  gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                             <  1:1836726/100‑1 (MQ=255)
                                                                  gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                             >  1:958609/1‑100 (MQ=255)
                                                                    tttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCa                           <  2:2245906/100‑1 (MQ=255)
                                                                     ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                          <  1:634829/100‑1 (MQ=255)
                                                                      tttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                         <  2:1217638/100‑1 (MQ=255)
                                                                          cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTCTGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAc                      >  2:445747/1‑99 (MQ=255)
                                                                          cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                     >  2:758354/1‑100 (MQ=255)
                                                                              gctGCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGACTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                 >  1:382132/3‑100 (MQ=37)
                                                                              gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                 <  1:1704667/100‑1 (MQ=255)
                                                                              gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCgg                  <  1:2170182/99‑1 (MQ=255)
                                                                               aTGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                 <  1:1381544/99‑1 (MQ=255)
                                                                                 gCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa              >  2:2661359/1‑100 (MQ=38)
                                                                                 gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa              <  2:2962190/100‑1 (MQ=255)
                                                                                  ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat             >  1:2670222/1‑100 (MQ=38)
                                                                                  ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCACTCAACCGCAGCCGAACTTCATCCACGCGGGCCat             >  1:857975/1‑100 (MQ=25)
                                                                                   cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata            >  1:936230/1‑100 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat           >  2:1600543/1‑100 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat           >  1:2448999/1‑100 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat           >  1:870044/1‑100 (MQ=38)
                                                                                    ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat           >  1:1135278/1‑100 (MQ=38)
                                                                                          aGCGTTTACGCCGCATCCGGCAGTCGTGTTTATGGGATTTTAATGGCTAAATAATCGCTCGCGCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  1:1337250/100‑1 (MQ=39)
                                                                                          aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  2:944032/100‑1 (MQ=255)
                                                                                          aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  2:2264146/100‑1 (MQ=255)
                                                                                          aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg     <  1:438793/100‑1 (MQ=255)
                                                                                            catttACGCCGTATCTGGCAGCCGTGTTTATGGTATCTCAATGGCTAAATAATCGCTCTCCCGCCGCCGAACTTCATCCACGTGGGCCATATCGTTTGAc   <  2:2882240/98‑1 (MQ=255)
                                                                                            cGTTTACGCCGCATCCGGCAGCGGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc   >  2:1760085/1‑100 (MQ=255)
                                                                                            cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc   <  1:1672739/100‑1 (MQ=255)
                                                                                             gTTTACGCCGCATCCGGCAGCTGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACt  <  1:1361587/100‑1 (MQ=255)
                                                                                             ctttACGCCGCATCCAGCAGCCGAGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTACATCCACGCGGGCCATATCGTTTGACt  >  2:1228827/2‑100 (MQ=255)
                                                                                                |                                                                                                
AGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACT  >  NC_000913/681604‑681796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: