Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,705 C→T 18.6% intergenic (+42/+18) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,7050CT18.6% 239.6 / 46.4 114intergenic (+42/+18)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base C (32/59);  new base T (11/10);  total (45/69)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-03

Only 100 of 124 total aligned reads displayed.

TTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCC  >  NC_000913/681606‑681804
                                                                                                   |                                                                                                   
tttAACGGTGCTGGTGATTGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACTATGTGTTTTTTGCGCCGATGCTGGATGCAGCGTTTAcgcc                                                                                                     <  2:198158/100‑1 (MQ=255)
tttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgcc                                                                                                     >  1:2264171/1‑100 (MQ=255)
 ttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccg                                                                                                    <  1:2727721/100‑1 (MQ=255)
      ggTGCTTGTGATAGGTTATTCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACTATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATcc                                                                                               <  1:2488153/100‑1 (MQ=255)
        tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGTGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                             <  2:2235840/100‑1 (MQ=255)
        tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                             <  2:58948/100‑1 (MQ=38)
        tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                             <  1:1841106/100‑1 (MQ=38)
        tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                             <  2:240127/100‑1 (MQ=38)
        tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                             <  2:1768826/100‑1 (MQ=38)
        tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                             <  1:1812307/100‑1 (MQ=38)
        tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCg                                                                                              <  2:1262349/99‑1 (MQ=255)
         gCTGGTGATAGGTTATGCTCTCGGCAAATTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                            <  2:766804/100‑1 (MQ=255)
         gCTGGTGATAGGTGATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGTATGCGGCGTAAACTCTGCATCCgg                                                                                             <  2:466901/99‑1 (MQ=255)
          cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCa                                                                                           <  1:1890559/100‑1 (MQ=38)
          cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                           <  2:1040266/100‑1 (MQ=255)
          cTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                           <  2:444057/100‑1 (MQ=255)
           tGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGc                                                                                            >  2:2142189/1‑98 (MQ=255)
            ggTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCGGGCAg                                                                                          <  2:1305924/99‑1 (MQ=255)
             gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCTATGCCGTATGCAGCGTTTACGCAGCATCCGGCATcc                                                                                        >  1:536845/1‑100 (MQ=255)
             gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                        >  1:1219539/1‑100 (MQ=255)
              tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCg                                                                                       >  1:3032263/1‑100 (MQ=255)
               gATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgt                                                                                      >  2:1905528/1‑100 (MQ=255)
                  aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACTCCGCATCCGGCAGCCGTGtt                                                                                   <  2:1182152/100‑1 (MQ=255)
                  aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                                   <  1:669082/100‑1 (MQ=255)
                   ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGttt                                                                                  <  1:1577821/100‑1 (MQ=255)
                    gTTAGGCTCTCGGCAAAATCGCGATTTTGTTTAGCTTTCGATGTGTGTTTGGCGCCTATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTa                                                                                 <  1:1765048/100‑1 (MQ=38)
                      tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                               <  2:2182723/100‑1 (MQ=255)
                      tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                               <  1:1709176/100‑1 (MQ=255)
                        tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                             <  1:2172997/100‑1 (MQ=255)
                        tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                             <  1:835727/100‑1 (MQ=255)
                        tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                             <  2:1355921/100‑1 (MQ=255)
                         gCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGa                                                                            <  2:148919/100‑1 (MQ=255)
                          ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                           >  1:1919778/1‑100 (MQ=255)
                          ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                           >  1:436308/1‑100 (MQ=255)
                          ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                           >  1:2913601/1‑100 (MQ=255)
                          ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCAGTGTTTATGGGa                                                                            >  1:1337053/1‑99 (MQ=255)
                           tctcGGCAAAATCGCGATGTTGTTTAGCTGACGTTGTGTTTTTTGTGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtc                                                                          <  1:1194825/100‑1 (MQ=255)
                             tcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtc                                                                          <  1:2180998/98‑1 (MQ=255)
                              cGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCa                                                                       >  2:726633/1‑100 (MQ=255)
                                gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                     >  1:2302919/1‑100 (MQ=255)
                                 cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGACAGCCGTGTTTATGGGATCTCAAt                                                                     >  1:2819836/1‑99 (MQ=255)
                                  aaaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                                   >  2:57055/1‑100 (MQ=255)
                                  aaaaTCGCGAGGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGAGGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                                   <  2:2811571/100‑1 (MQ=255)
                                     aTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTa                                                                <  2:1452034/100‑1 (MQ=255)
                                      tcgttaTGTTTTTTATTTGACTATTTGTTTTTTGCGCCGAGTCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGc                                                                  <  1:1341139/92‑1 (MQ=37)
                                      tCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTATACGCAGCATCCGGCAGCCGTGTTTATTGAAACTCAATGGCTa                                                                >  1:502954/1‑99 (MQ=255)
                                         cgATGGTGTTTCGGTGACGATGTGTTTTTTGCGCCGATGGCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                             <  2:625774/99‑1 (MQ=39)
                                           aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                                          <  1:1905551/100‑1 (MQ=255)
                                           aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACACCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaataat                                                          >  1:2421653/1‑100 (MQ=255)
                                            tgttgtTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGTGATCTCAATGGCTAAATAAtc                                                         <  1:1265807/100‑1 (MQ=255)
                                             gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                        <  1:692760/100‑1 (MQ=255)
                                              ttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgt                                                       <  2:1358167/100‑2 (MQ=255)
                                                gttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctc                                                     >  1:1227829/1‑100 (MQ=255)
                                                 tttAGCTGGCGATGTCTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCTGTGTTTATGGGATCTGTATGGCTAAATAAtcg                                                        <  2:2389790/96‑1 (MQ=38)
                                                  ttAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctcgc                                                   <  2:2512453/100‑1 (MQ=255)
                                                   tAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcc                                                  <  1:1594827/100‑1 (MQ=255)
                                                     gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                                >  1:430496/1‑100 (MQ=255)
                                                       tGACGATTTTTTTTTTGCGTCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                              <  1:1323041/100‑1 (MQ=255)
                                                       tGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                              >  1:269591/1‑100 (MQ=255)
                                                       tGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                              >  1:1084891/1‑100 (MQ=255)
                                                        gACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgcc                                              >  1:219226/1‑99 (MQ=255)
                                                          cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                           >  1:799391/1‑100 (MQ=255)
                                                          cGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGAATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                           >  1:1001521/1‑100 (MQ=255)
                                                           gATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgccg                                          >  2:1052042/1‑100 (MQ=255)
                                                           gATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgccg                                          <  2:879141/100‑1 (MQ=255)
                                                            aTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGa                                         <  1:1100202/100‑1 (MQ=255)
                                                             tgtgTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGaa                                        >  1:944038/1‑100 (MQ=255)
                                                              gtgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAAc                                       <  2:510252/100‑1 (MQ=255)
                                                              gtgtTTGTTGGGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAAc                                       <  2:2853121/100‑1 (MQ=255)
                                                                gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                                     >  1:958609/1‑100 (MQ=255)
                                                                  tttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCa                                   <  2:2245906/100‑1 (MQ=255)
                                                                   ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                                  <  1:634829/100‑1 (MQ=255)
                                                                    tttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                                 <  2:1217638/100‑1 (MQ=255)
                                                                        cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTCTGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAc                              >  2:445747/1‑99 (MQ=255)
                                                                        cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcg                             >  2:758354/1‑100 (MQ=255)
                                                                            gctGCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGACTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                         >  1:382132/3‑100 (MQ=37)
                                                                            gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                         <  1:1704667/100‑1 (MQ=255)
                                                                            gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCgg                          <  1:2170182/99‑1 (MQ=255)
                                                                            gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGAATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                         >  1:2829638/1‑100 (MQ=255)
                                                                             aTGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                         <  1:1381544/99‑1 (MQ=255)
                                                                               gCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa                      >  2:2661359/1‑100 (MQ=38)
                                                                               gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa                      <  2:2962190/100‑1 (MQ=255)
                                                                                ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat                     >  1:2670222/1‑100 (MQ=38)
                                                                                ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCACTCAACCGCAGCCGAACTTCATCCACGCGGGCCat                     >  1:857975/1‑100 (MQ=25)
                                                                                 cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata                    >  1:936230/1‑100 (MQ=38)
                                                                                 cGGATGCGGCGTAAACGCTGCATCCAGCAGCCGTGTTTATGGGATCGCAATGGCTAAATCATCGCTCGCCCGCCGCAGAACTTCATCCa                               >  2:1580197/1‑89 (MQ=25)
                                                                                  ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                   >  1:2448999/1‑100 (MQ=38)
                                                                                  ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                   >  1:1135278/1‑100 (MQ=38)
                                                                                  ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                   >  1:870044/1‑100 (MQ=38)
                                                                                        aGCGTTTACGCCGCATCCGGCAGTCGTGTTTATGGGATTTTAATGGCTAAATAATCGCTCGCGCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg             <  1:1337250/100‑1 (MQ=39)
                                                                                        aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg             <  2:944032/100‑1 (MQ=255)
                                                                                        aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg             <  1:438793/100‑1 (MQ=255)
                                                                                        aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg             <  2:2264146/100‑1 (MQ=255)
                                                                                          catttACGCCGTATCTGGCAGCCGTGTTTATGGTATCTCAATGGCTAAATAATCGCTCTCCCGCCGCCGAACTTCATCCACGTGGGCCATATCGTTTGAc           <  2:2882240/98‑1 (MQ=255)
                                                                                          cGTTTACGCCGCATCCGGCAGCGGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc           >  2:1760085/1‑100 (MQ=255)
                                                                                               aCGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGc        <  2:1524034/98‑1 (MQ=255)
                                                                                               aCGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAAATTCATACACGCGGGCCATATCGTTTGACTGCaa      >  1:92682/1‑100 (MQ=255)
                                                                                                 gccgcATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCAGGCCATATCGTTTGACTGCAAcg    >  1:1127445/1‑100 (MQ=255)
                                                                                                  ccgcATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcgc   >  1:886922/1‑100 (MQ=255)
                                                                                                   cgcATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcgcc  <  1:143631/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCC  >  NC_000913/681606‑681804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: