BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	712,150	A→T	100%	L218Q (CTG→CAG)	ybfF ←	acyl‑CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	712,150	0	A	T	100.0%	336.4 / ‑6.7	105	L218Q (CTG→CAG)	ybfF	acyl‑CoA esterase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (55/49); minor base C (1/0); total (56/49)

Only 100 of 105 total aligned reads displayed.

GCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTA  >  NC_000913/712053‑712246

ncGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGt                                                                                                >  2:574027/2‑100 (MQ=255)

gCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGt                                                                                                >  2:480049/1‑100 (MQ=255)

 cGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTa                                                                                               <  1:2780540/100‑1 (MQ=255)

  gACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTaa                                                                                              >  1:949282/1‑100 (MQ=255)

  gACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTaa                                                                                              >  2:316742/1‑100 (MQ=255)

  gACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACAAGCACCTGCAATAACATGCACCCGTACCTGTGGAAATTGAGCCTGTa                                                                                               >  1:1804605/1‑99 (MQ=255)

   aCGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTaaa                                                                                             <  2:718333/100‑1 (MQ=255)

    cGGATAGCGCGTAATACCGCATTCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAat                                                                                            <  1:1388403/100‑1 (MQ=255)

        tAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAGatca                                                                                          <  2:2948773/98‑1 (MQ=255)

         aGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAAtcatca                                                                                       >  2:431792/1‑100 (MQ=255)

           cgcgTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGTCTGTGGAAATTGAGCCTGTAAATCATCACg                                                                                     <  2:2220575/100‑1 (MQ=255)

            gcgTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACgg                                                                                    >  1:1150868/1‑100 (MQ=255)

             cgTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGt                                                                                   <  2:635309/100‑1 (MQ=255)

              gTAATACCGCATCCGGTTTTTCTGCATGGACTCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTa                                                                                  <  1:835854/100‑1 (MQ=255)

              gTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTa                                                                                  <  2:803261/100‑1 (MQ=255)

              gTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTa                                                                                  <  1:2299204/100‑1 (MQ=255)

               tAATACCGCATTCGGTTTTTCTGCATGTACTCTGTTTCCCGCGTCTGCAATCACATGCGCACGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTa                                                                                  <  1:1032220/99‑1 (MQ=255)

               tAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTAc                                                                                 >  2:87933/1‑100 (MQ=255)

                 aTACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTg                                                                               <  2:2663061/100‑1 (MQ=255)

                 aTACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTg                                                                               <  2:233752/100‑1 (MQ=255)

                  tACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGc                                                                              >  1:3040331/1‑100 (MQ=255)

                  tACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGc                                                                              >  2:2161789/1‑100 (MQ=255)

                   aCCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCt                                                                             >  1:727405/1‑100 (MQ=255)

                    ccGCATCCCGTTTTTCAGCATGGACCCAGAGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAACTTGAGCCTCTAAATCATCACGGTACTGc                                                                              >  2:1056859/1‑98 (MQ=255)

                     cGCATCCGGTTTTTCAGCATGGACCAAGTGACCCGAGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTCAATCATCACGGTACTGCTc                                                                            >  2:2334999/1‑99 (MQ=255)

                         tCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGACTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTaa                                                                       >  1:873264/1‑100 (MQ=255)

                          ccGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAAc                                                                      >  1:169466/1‑100 (MQ=255)

                           cGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACa                                                                     >  2:2942503/1‑100 (MQ=255)

                           cGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACa                                                                     >  2:1923448/1‑100 (MQ=255)

                           cGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACa                                                                     >  1:2113530/1‑100 (MQ=255)

                           cGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTAAGCCTGTAAATCATCACGGTACTGCTCGCTAACa                                                                     >  1:2495342/1‑100 (MQ=255)

                             gTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATa                                                                   <  1:2550470/100‑1 (MQ=255)

                             gTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATa                                                                   >  1:1252845/1‑100 (MQ=255)

                                  tCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATAATAACGGTACTGCTAGCTAAAATACGga                                                               >  1:2137963/1‑99 (MQ=255)

                                    aGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGaa                                                            <  2:1562376/100‑1 (MQ=255)

                                     gCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCATTGTACTGCTCGCTAACATACGGAGAAt                                                           >  1:2155729/1‑100 (MQ=255)

                                     gCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAAt                                                           >  2:152900/1‑100 (MQ=255)

                                     gCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAAt                                                           >  2:2532075/1‑100 (MQ=255)

                                       aTGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTg                                                         <  1:22279/100‑1 (MQ=255)

                                          gACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGTAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTgccg                                                      <  1:855969/100‑1 (MQ=255)

                                          gACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTgccg                                                      <  1:1948625/100‑1 (MQ=255)

                                          gACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTgccg                                                      >  1:49490/1‑100 (MQ=255)

                                           aCCCAGTGACCCGCGCCTGCAATCAAATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTgccg                                                      <  2:2805573/99‑1 (MQ=255)

                                            cccAGTGACCCGCGCCTGCAATCACTTGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTgccgcc                                                    <  1:283896/100‑1 (MQ=255)

                                            cccAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTgccgcc                                                    <  1:1134948/100‑1 (MQ=255)

                                            cccAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATAACGGTACTGCTCGCTAACATACGGAGAATTgccgcc                                                    >  1:238832/1‑100 (MQ=255)

                                             ccAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCa                                                   <  2:2193210/100‑1 (MQ=255)

                                              cAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAg                                                  >  1:1778996/1‑100 (MQ=255)

                                                gTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAggg                                                <  2:2196275/100‑1 (MQ=255)

                                                gTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAggg                                                >  2:578323/1‑100 (MQ=255)

                                                  gACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGAt                                              >  1:1930214/1‑100 (MQ=255)

                                                   aCCCGCGCCTGCAATCACATGCGCCCGTTCCTGTGGAAATTTAGCCTGTAAATCATCACGGTAATACTCACTAACATACGGAGAATTGCCGCCAGGGATa                                             >  1:2293632/1‑100 (MQ=255)

                                                         gcCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAAcagg                                       <  1:1623559/100‑1 (MQ=255)

                                                          ccTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAAcaggg                                      <  1:2226976/100‑1 (MQ=255)

                                                          ccTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAAcaggg                                      >  2:2853032/1‑100 (MQ=255)

                                                             gCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAAcagggcag                                   >  2:37573/1‑100 (MQ=255)

                                                              cAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAAcagggcagg                                  >  2:2770790/1‑100 (MQ=255)

                                                               aaTCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAAcagggcaggg                                 <  1:989321/100‑1 (MQ=255)

                                                                aTCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAAcagggcaggga                                <  1:2272985/100‑2 (MQ=255)

                                                                aTCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAAcagggcaggg                                 >  1:2252358/1‑99 (MQ=255)

                                                                 tCACATGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTg                               <  2:948786/100‑1 (MQ=255)

                                                                     aTGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATcc                           >  2:1423426/1‑100 (MQ=255)

                                                                     aTGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATcc                           <  1:1957393/100‑1 (MQ=255)

                                                                      tGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATccc                          >  2:1263355/1‑100 (MQ=255)

                                                                      tGCGCCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATccc                          >  1:153095/1‑100 (MQ=255)

                                                                       gcgcCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCa                         <  2:49490/100‑1 (MQ=255)

                                                                        cgcCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCAt                        >  2:847078/1‑100 (MQ=255)

                                                                         gcCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATg                       >  2:1676613/1‑100 (MQ=255)

                                                                         gcCCGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGATACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATg                       >  2:1866164/1‑100 (MQ=255)

                                                                          cccGTGCCTGTGGAAATTGAGACTGTAAATCATCACGGTACTGCTCGCTAAAATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGc                      >  2:1442336/1‑100 (MQ=255)

                                                                           ccGTGCCTGTGGAGATTGAGCCTGTAAATCATCACGGTTCTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGc                      >  2:1874027/1‑99 (MQ=255)

                                                                            cGTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGc                      <  2:2590967/98‑1 (MQ=255)

                                                                             gTGCCTGTGGAAATTTAGACTGTAAATCATCACTGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGCGATCCCATGCCgg                   <  2:1230304/100‑1 (MQ=255)

                                                                             gTGCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCCCTAACATACGTAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCgg                   <  2:410775/100‑1 (MQ=255)

                                                                               gCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGa                 <  1:578326/100‑1 (MQ=255)

                                                                               gCCTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCACCAGGGATAAACAGAGCAGGGTGATCCCATGCCGGGa                 >  1:1231837/1‑100 (MQ=255)

                                                                                ccTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGAt                <  1:1705124/100‑1 (MQ=255)

                                                                                ccTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGAt                >  1:2549934/1‑100 (MQ=255)

                                                                                ccTGTGGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGAt                >  1:885255/1‑100 (MQ=255)

                                                                                  tgtgGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGAttt              <  2:148449/100‑1 (MQ=255)

                                                                                  tgtgGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCACCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGAtt               >  1:147889/1‑99 (MQ=255)

                                                                                   gtgGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAAGGATAAACAGGGCAGGGTGATCCCATGCCGGGAttt              >  1:338803/1‑99 (MQ=255)

                                                                                    tgGAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCATGGGGATCCCATGCCGGGAttt              >  2:2621342/1‑98 (MQ=255)

                                                                                      gAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTtctc          <  1:1650856/100‑1 (MQ=255)

                                                                                      gAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAAGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTtctc          >  1:1006730/1‑100 (MQ=255)

                                                                                      gAAATTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGACAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTtct           >  1:879685/1‑99 (MQ=255)

                                                                                       aaaTTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGTAGAATTGCCGCCAGTGATAAACAGGGCAGGGTTATCCCATGCCGGGATTTTCTcc         <  2:2137940/100‑1 (MQ=255)

                                                                                       aaaTTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTcc         <  2:1697865/100‑1 (MQ=255)

                                                                                       aaaTTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACAGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTcc         <  2:1485066/100‑1 (MQ=255)

                                                                                        aaTTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTccc        >  1:2739822/1‑100 (MQ=255)

                                                                                         aTTGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCa       <  2:239564/100‑1 (MQ=255)

                                                                                          ttGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCaa      <  2:1449062/100‑1 (MQ=255)

                                                                                           tGAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAAc     <  1:1291070/100‑1 (MQ=255)

                                                                                            gAGCCTGTAAATCATCACGGTACTGGTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAAcc    <  2:3041543/100‑1 (MQ=255)

                                                                                            gAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAAcc    <  2:1887451/100‑1 (MQ=255)

                                                                                            gAGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAAcc    >  1:828169/1‑100 (MQ=255)

                                                                                             aGCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCt   <  2:238830/100‑1 (MQ=255)

                                                                                              gCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTa  >  2:1429678/1‑100 (MQ=255)

                                                                                              gCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTa  >  2:1907791/1‑100 (MQ=255)

                                                                                              gCCTGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGAGCAGGGTGATCACATGCCGGGAATTTCTCCCAACCTa  >  1:2001165/1‑100 (MQ=255)

GCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTA  >  NC_000913/712053‑712246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`