Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 824,573 T→A 15.2% intergenic (‑76/‑57) ybhP ← / → ybhQ endo/exonuclease/phosphatase family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913824,5730TA15.2% 272.3 / 36.8 105intergenic (‑76/‑57)ybhP/ybhQendo/exonuclease/phosphatase family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (50/39);  new base A (6/10);  total (56/49)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 109 total aligned reads displayed.

ATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGT  >  NC_000913/824476‑824671
                                                                                                 |                                                                                                  
aTTGTTGTTTTTGATCTGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAttt                                                                                                   <  2:1947621/99‑1 (MQ=255)
 ttgttgTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTc                                                                                                 >  2:1462426/1‑100 (MQ=255)
   gttgtGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCg                                                                                               >  1:2918380/1‑100 (MQ=255)
      gtgtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaa                                                                                            >  1:1722797/1‑100 (MQ=255)
       tgtTTTATCGGGTATAATTTTGCTGTCTTTGGCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaag                                                                                           <  2:2378515/100‑2 (MQ=255)
       tgtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCTTTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaag                                                                                           <  1:93804/100‑2 (MQ=255)
       tgtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                           <  2:1907093/100‑1 (MQ=255)
       tgtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaag                                                                                           <  1:2176537/100‑2 (MQ=255)
        gtTTGATCGGGCATAATTTTCCTGTCTTTGCCTTTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaaga                                                                                          <  1:936670/100‑3 (MQ=255)
        gtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGaaaa                                                                                           >  2:379113/1‑99 (MQ=255)
        gtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAg                                                                                          <  1:546253/100‑1 (MQ=255)
        gtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAg                                                                                          >  1:2997754/1‑100 (MQ=255)
         tttGATTGGGGATAATTTGCCTGTCGTTGCCTCTTATCTCATTGAAATAGTGTAGGCAGTGTGTCAAAAAGTTGTGGTCTTACGGAAAATTCAGTaag                                                                                           <  2:1423078/98‑2 (MQ=255)
         tttGATCGGGCATAATTTTCCTGTCTTTGTCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaagac                                                                                         <  1:364725/100‑4 (MQ=255)
          ttGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaagacc                                                                                        <  1:3042049/100‑5 (MQ=255)
          ttGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaagacc                                                                                        <  2:731182/100‑5 (MQ=255)
           tGATCGTGCATAATTTTCCTGTCTTTGCCTCTTATTTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTc                                                                                       <  1:2072897/100‑1 (MQ=255)
            gATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCg                                                                                      <  2:2524102/100‑1 (MQ=255)
              tCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGt                                                                                    <  1:2652555/100‑1 (MQ=255)
              tCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGt                                                                                    <  2:2942027/100‑1 (MQ=255)
                gggCATAATGTTGCTGTCTTTGCGGCTTATCGCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaagaccgcac                                                                                    <  2:123764/98‑9 (MQ=25)
                 ggCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTAct                                                                                 >  1:2072670/1‑100 (MQ=255)
                  gCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                >  1:1339507/1‑100 (MQ=255)
                     tAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGa                                                                             >  1:2510368/1‑100 (MQ=255)
                      aaTTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGag                                                                            <  1:1626422/100‑1 (MQ=255)
                       aTTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagt                                                                           <  1:1199076/100‑1 (MQ=255)
                       aTTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCATCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGag                                                                            >  1:2408271/1‑99 (MQ=255)
                       aTTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAATCAAAAAAACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagt                                                                           <  2:467004/100‑1 (MQ=255)
                        ttttCCTGTCTTTGCCTCTCATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagta                                                                          <  1:498668/100‑1 (MQ=255)
                          ttCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaag                                                                        >  1:1134183/1‑100 (MQ=255)
                            ccTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaagta                                                                      <  2:2918348/100‑1 (MQ=255)
                              tGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGAGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTaga                                                                    >  2:1861923/1‑100 (MQ=255)
                               gTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTagag                                                                   >  2:2607911/1‑100 (MQ=255)
                               gTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCAGTAAAGTTCGGTACTATGAGTAAGTagag                                                                   >  1:2940028/1‑100 (MQ=255)
                                 cTTTGCCTCTTATCTCATTGAAATAGTGCAGTCGGTGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGAACTCTGAGTAAGTAGAGAt                                                                 >  1:1937782/1‑100 (MQ=255)
                                  tttGCCTCTTATCTCATTGAAATAGTGTTGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATa                                                                <  1:1702840/100‑1 (MQ=255)
                                  tttGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGAt                                                                 <  2:1700033/99‑1 (MQ=255)
                                  tttGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATa                                                                >  2:1565256/1‑100 (MQ=255)
                                     gCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAt                                                             <  2:2144741/100‑1 (MQ=255)
                                     gCCTCTTATCTCATTGAAATAGTGTAGTCGGCGACACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATaaa                                                              <  2:469646/99‑1 (MQ=255)
                                           tacctcATTGAAATAGTGTAGTCTGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGAAGAGATAAATTCTTCa                                                       <  2:2283240/97‑1 (MQ=255)
                                            aTCTCATTTAAATAGTGTAGTTGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAg                                                      <  1:275988/100‑1 (MQ=255)
                                            aTCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAg                                                      >  2:1257892/1‑100 (MQ=255)
                                             tctcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTTCGGTTTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAgg                                                     <  1:139202/100‑1 (MQ=255)
                                             tctcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAgg                                                     <  2:2006666/100‑1 (MQ=255)
                                             tctcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAgg                                                     >  1:3001577/1‑100 (MQ=255)
                                              ctcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGga                                                    <  1:2944127/100‑1 (MQ=255)
                                                cATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGtaga                                                  <  1:904299/100‑1 (MQ=255)
                                                cATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGgaga                                                  <  2:2462255/100‑1 (MQ=255)
                                                cATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGAAAGTAGAGATAAATTCTTCAGgaga                                                  >  1:229098/1‑100 (MQ=255)
                                                cATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGATCGGTACTATGAGTAAGTAGAGATAAATTCTTCAGgaga                                                  >  1:2603782/1‑100 (MQ=255)
                                                  ttGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGgagaga                                                >  2:1347887/1‑100 (MQ=255)
                                                  ttGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGgagaga                                                >  1:2692615/1‑100 (MQ=255)
                                                    gAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAg                                              >  1:3043468/1‑100 (MQ=255)
                                                    gAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAg                                              >  2:615235/1‑100 (MQ=255)
                                                    gAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAg                                              >  2:1267543/1‑100 (MQ=255)
                                                       aTAGTGTAGTCGGCGTCACAAAAATGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCa                                           <  1:816387/100‑1 (MQ=255)
                                                         aGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATg                                         <  2:1722777/100‑1 (MQ=255)
                                                         aGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATg                                         >  1:1678496/1‑100 (MQ=255)
                                                             tAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGt                                     <  2:2218996/100‑1 (MQ=255)
                                                              aGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTg                                    >  2:2983219/1‑100 (MQ=255)
                                                              aGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGGAAAATTCAGTACTCTGAGGAAGTAGAGATAAATTCTTTAGGTGAGAAGCCATGAAgg                                     >  2:755445/1‑98 (MQ=255)
                                                               gTCTGCTTCACAAAAAGTTGCGGTCTTACGTAATTTTCCGTAAAGTTCGGTACTCTGAGTAATTAGAGATAAATTCTTCAGGATAGAAGCCATGAAGTgg                                   <  2:2096141/100‑1 (MQ=255)
                                                               gTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTgg                                   >  1:690946/1‑100 (MQ=255)
                                                                tCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGc                                  <  2:1522078/100‑1 (MQ=255)
                                                                   gCGTCACAAAAAGGTGCGGTCTTACGGAATTGTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTgg                                   <  2:1339495/96‑1 (MQ=255)
                                                                    cGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGcaaca                              >  2:3030947/1‑100 (MQ=255)
                                                                     gTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGcaacaa                             >  1:2523172/1‑100 (MQ=255)
                                                                     gTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGcaaca                              <  2:1377382/99‑1 (MQ=255)
                                                                     gTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAATAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGcaacaa                             >  2:2201079/1‑100 (MQ=255)
                                                                     gTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTATGAGTAAGTAGAGATAAATTCTTAAGGAGAGAAGCCATGAAGTGGcaacaa                             >  2:136345/1‑100 (MQ=255)
                                                                       cacaAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACg                           >  1:1644254/1‑100 (MQ=255)
                                                                         caAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACgtg                         >  2:1732664/1‑100 (MQ=255)
                                                                         caAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACgtg                         >  1:1960842/1‑100 (MQ=255)
                                                                             aaagTGCTGTCTTACGTAATTTTCCGTAAATTTCGGTACTCTTAGTAATTATAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCg                     <  1:2579089/97‑1 (MQ=255)
                                                                               ggTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCgtg                   <  1:3020078/100‑1 (MQ=255)
                                                                                 ccgaactttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTc                 >  1:2692915/8‑100 (MQ=255)
                                                                                  gCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCg                <  2:2836464/100‑1 (MQ=255)
                                                                                  cgaactttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCg                >  1:1161169/7‑100 (MQ=255)
                                                                                  cgaactttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAACCATGAAGTGAAAACAACGTGTTCGTGTCg                >  1:441527/7‑100 (MQ=255)
                                                                                   gaactttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGACAAAAACGTGTTCGTGTCGc               >  1:1885439/6‑100 (MQ=255)
                                                                                   cGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGc               <  2:2510339/100‑1 (MQ=255)
                                                                                    ggTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCa              >  1:622455/1‑100 (MQ=255)
                                                                                    ggTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTATTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCa              >  1:827002/1‑100 (MQ=255)
                                                                                     gTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGATATAAATTCTTAAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCaa             >  2:1917183/1‑100 (MQ=255)
                                                                                     gTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAATGGCAACAACGAATTCGTATCGCaa             >  1:2177092/1‑100 (MQ=255)
                                                                                     actttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTGCGTGTCGCaa             >  2:1995759/4‑100 (MQ=255)
                                                                                       cTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACg           <  1:975718/100‑1 (MQ=255)
                                                                                        ttACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACgg          <  1:2823263/100‑1 (MQ=255)
                                                                                         tACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACggg         <  1:615180/100‑1 (MQ=255)
                                                                                           cGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGt        >  1:1206626/1‑99 (MQ=255)
                                                                                           cGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTAGTGTCGCAACGGGTc       >  1:718114/1‑100 (MQ=255)
                                                                                            ggAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCt      >  1:2505672/1‑100 (MQ=255)
                                                                                             gAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTa     >  1:1940808/1‑100 (MQ=255)
                                                                                             gAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTa     >  1:1803600/1‑100 (MQ=255)
                                                                                             gAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTa     >  1:1801657/1‑100 (MQ=255)
                                                                                               aTTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAg   >  1:558464/1‑100 (MQ=255)
                                                                                                ttttCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGt  >  2:2455037/1‑100 (MQ=255)
                                                                                                ttttCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGt  <  1:1347899/100‑1 (MQ=255)
                                                                                                ttttCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGt  >  2:1284509/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
ATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGT  >  NC_000913/824476‑824671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: