Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 834,352 A→T 100% Q428L (CAG→CTG)  dinG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913834,3520AT98.9% 339.5 / ‑4.2 105Q428L (CAG→CTG) dinGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (53/50);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCAGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCTCCGC  >  NC_000913/834255‑834448
                                                                                                 |                                                                                                
 tCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGgctgct                                                                                               >  1:2571067/1‑100 (MQ=255)
   gAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTac                                                                                             >  2:2736965/1‑100 (MQ=255)
    aGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTaca                                                                                            <  1:589436/100‑1 (MQ=255)
    aGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGAGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTaca                                                                                            >  1:2143433/1‑100 (MQ=255)
     ggCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTacac                                                                                           >  2:3006396/1‑100 (MQ=255)
      gcgcAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACAcc                                                                                          <  2:1398630/100‑1 (MQ=255)
      gcgcAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACAcc                                                                                          >  2:1993600/1‑100 (MQ=255)
       cgcAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACct                                                                                         >  1:1186306/1‑100 (MQ=255)
        gcaaagcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACctc                                                                                        >  2:2011669/1‑100 (MQ=255)
         caaagcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACctct                                                                                       >  2:1061981/1‑100 (MQ=255)
          aaagcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTg                                                                                      <  2:79549/100‑1 (MQ=255)
          aaaacaagCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGTGCGCGAAGGGCTGCTACACCTCTg                                                                                      <  2:2798699/100‑1 (MQ=255)
           aagcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTgg                                                                                     <  1:1340212/100‑1 (MQ=255)
             gcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGtt                                                                                   >  2:952978/1‑100 (MQ=255)
               aaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTc                                                                                 <  2:1174035/100‑1 (MQ=255)
                aaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACAACTCTGGTTTCa                                                                                >  2:1739204/1‑100 (MQ=255)
                  ctctGGCGGCTGGCTTCGTTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCGCTGGtttt                                                                                 <  2:2886611/97‑2 (MQ=255)
                   tctGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTg                                                                             <  1:289371/100‑1 (MQ=255)
                     tGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCAGCTACACCTCTGGTTTCACTGCg                                                                           <  2:2468885/100‑1 (MQ=255)
                      ggcggcTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGt                                                                          <  1:330509/100‑1 (MQ=255)
                       gcggcTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTg                                                                         <  2:2832672/100‑1 (MQ=255)
                       gcggcTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTg                                                                         <  2:1976555/100‑1 (MQ=255)
                       gcggcTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTg                                                                         <  1:2885476/100‑1 (MQ=255)
                       gcggcTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTg                                                                         <  1:889986/100‑1 (MQ=255)
                        cggcTGGCTTCGCTGGCGCAATCTTACGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTgg                                                                        >  2:1663198/1‑100 (MQ=255)
                         ggctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTggg                                                                       <  1:1471225/100‑1 (MQ=255)
                         ggctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTggg                                                                       <  1:862839/100‑1 (MQ=255)
                         ggctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTggg                                                                       <  1:372231/100‑1 (MQ=255)
                         ggctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACGGCGTggg                                                                       <  1:1582881/100‑1 (MQ=255)
                         ggctggcgTCGCTGGCGCAATCTTCCGGTGCACCAGTGACCATATTGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTggg                                                                       <  2:346151/100‑1 (MQ=255)
                           ctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGaa                                                                     <  1:2527840/100‑1 (MQ=255)
                             gtctTCGCTGGCGCAATCTTCCTGTGCACCTGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGTCTGCTACACCTCTGTTTTCACTGCGTggg                                                                       <  2:604581/94‑1 (MQ=255)
                             ggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATa                                                                   >  2:174293/1‑100 (MQ=255)
                             ggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATa                                                                   >  1:1259517/1‑100 (MQ=255)
                             ggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATa                                                                   >  2:1917899/1‑100 (MQ=255)
                             ggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATa                                                                   <  1:1007838/100‑1 (MQ=255)
                               ctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACg                                                                 <  2:170440/100‑1 (MQ=255)
                               ctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACg                                                                 <  2:685141/100‑1 (MQ=255)
                                ttCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACgt                                                                <  2:47141/100‑1 (MQ=255)
                                ttCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACg                                                                 <  2:2596483/99‑1 (MQ=255)
                                 tCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACgtg                                                               >  2:2141621/1‑100 (MQ=255)
                                  cGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGTTTTCACTGCGTGGGAATACgtg                                                               <  2:834741/99‑1 (MQ=255)
                                   gCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGCAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACgtgt                                                              >  2:1935120/1‑99 (MQ=255)
                                    cTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCa                                                            >  2:2015916/1‑100 (MQ=255)
                                      ggCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGc                                                          >  1:2715665/1‑100 (MQ=255)
                                      ggCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGc                                                          >  2:1244252/1‑100 (MQ=255)
                                       gcgcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGc                                                          >  1:1025855/1‑99 (MQ=255)
                                        cgcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGa                                                        >  2:1568055/1‑100 (MQ=255)
                                        cgcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGa                                                        >  2:2434929/1‑100 (MQ=255)
                                        cgcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAAGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGa                                                        >  1:29836/1‑100 (MQ=255)
                                         gcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGAt                                                       >  1:1363106/1‑100 (MQ=255)
                                         gcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGAt                                                       >  2:1827140/1‑100 (MQ=255)
                                         cttccgatctggTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGAt                                                       <  2:1367097/90‑1 (MQ=255)
                                          cAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATc                                                      >  2:1231896/1‑100 (MQ=255)
                                          cAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATc                                                      >  1:1242123/1‑100 (MQ=255)
                                          cAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATc                                                      >  1:2770414/1‑100 (MQ=255)
                                          cAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATc                                                      >  1:826362/1‑100 (MQ=255)
                                           aaTCTTCCGGTGCACCGGTGATCAAATGGGCGAGGCGGGAAGAGCGCGAAGGGCTGCTAAACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATc                                                      <  1:1068908/99‑1 (MQ=255)
                                           aaTCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCa                                                     <  1:804412/100‑1 (MQ=255)
                                               ttCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAATGGCTGCTACACCTCTGGTTTCAATGCGTGGGAATACGTGTCAGCGATCAGCTg                                                 >  1:1093372/1‑100 (MQ=255)
                                                tCCGGTGCACCGCTGACCAAATGGCCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTTGGAATACGTGTCACCGATCAGCTgg                                                <  2:2531722/100‑1 (MQ=255)
                                                  cGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAATGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGaa                                              >  1:532767/1‑100 (MQ=255)
                                                   ggTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGAtagatcggaag                                             >  1:1367109/1‑90 (MQ=255)
                                                   ggTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCAGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGaaa                                             >  2:898432/1‑100 (MQ=255)
                                                   ggTGCACCGCTGACCAAATGGTCAACGATGGAAGAGCTCGAAGGGCTGATACTCCTCTGGTTTCACTGCGTGGGATTACGTGTCAGCGATCa                                                     <  2:2761900/92‑1 (MQ=255)
                                                     tGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAgg                                           >  2:2635480/1‑100 (MQ=255)
                                                     tGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAgg                                           <  2:1132234/100‑1 (MQ=255)
                                                     tGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAgg                                           >  2:388531/1‑100 (MQ=255)
                                                      gCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgc                                          <  2:1109437/100‑1 (MQ=255)
                                                      gCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgc                                          >  2:275053/1‑100 (MQ=255)
                                                      gCACCGGTGACCAAATGGGCGACGCAGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAgg                                           >  2:2969351/1‑99 (MQ=255)
                                                       cACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgct                                         >  1:701702/1‑100 (MQ=255)
                                                        aCCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgctg                                        >  1:2089519/1‑100 (MQ=255)
                                                        aCCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgctg                                        >  2:1937312/1‑100 (MQ=255)
                                                         ccGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgctgc                                       >  1:2955787/1‑100 (MQ=255)
                                                         ccGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCAATACGTAGGAATACGTGTCAGCGATCAGCTGGAAAGgctgc                                       >  1:874746/1‑100 (MQ=255)
                                                          cGGTGACCAAATGGGCGACGCGGGATGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgctgc                                       <  1:1244264/99‑1 (MQ=255)
                                                          cGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgc                                          <  2:1909266/96‑1 (MQ=255)
                                                           ggTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGctgctg                                     >  2:2718602/1‑100 (MQ=255)
                                                           ggTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGctgctg                                     >  2:2935213/1‑100 (MQ=255)
                                                           ggTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGctgctg                                     >  2:2719309/1‑100 (MQ=255)
                                                            gTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCtgt                                    >  1:185572/1‑100 (MQ=255)
                                                               aCCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGgc                                 >  2:1225257/1‑100 (MQ=255)
                                                                   aaTGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACTTCTGGTTTCACTGGGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAg                             <  1:231897/100‑1 (MQ=255)
                                                                   aaTGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATATGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAg                             <  2:896984/100‑1 (MQ=255)
                                                                   aaTGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAg                             <  2:302486/100‑1 (MQ=255)
                                                                   aaTGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAg                             <  2:2063076/100‑1 (MQ=255)
                                                                   aaTGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAg                             <  2:1659646/100‑1 (MQ=255)
                                                                     tGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTa                           <  1:2141644/100‑1 (MQ=255)
                                                                       ggcggcGGGGGAAGAGCGCGAAGGGCTTCTTCCCCGCTGGTTTCACTGCGTGTGAATATGTGTCAGCGGTCAGCTGGAAAGGCTGCTGTGGCGCAg                             <  2:1106528/96‑1 (MQ=255)
                                                                         cGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTcc                       <  2:2486763/100‑1 (MQ=255)
                                                                                ggAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACatt                >  1:2303762/1‑100 (MQ=255)
                                                                                 gAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGcacagca               <  2:125631/100‑4 (MQ=255)
                                                                                  nagagCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACattat              >  2:2591801/2‑100 (MQ=255)
                                                                                    gagCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGTAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTg            <  1:609899/100‑1 (MQ=255)
                                                                                       cgcgAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCa         <  2:1930239/100‑1 (MQ=255)
                                                                                        gcgAATGGCTGCTACACCTTTGGTTTCACTGCGTGGTAATACGTCTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTc          <  2:2667719/98‑1 (MQ=255)
                                                                                          gAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCt      <  1:2434958/100‑1 (MQ=255)
                                                                                              ggCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCTCCGc  >  2:448279/1‑100 (MQ=255)
                                                                                              ggCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCTCCGc  >  2:1973586/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCAGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCTCCGC  >  NC_000913/834255‑834448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: