Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 933,387 C→T 100% A55V (GCC→GTC)  ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913933,3870CT97.0% 289.5 / ‑2.3 94A55V (GCC→GTC) ftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (49/42);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCC  >  NC_000913/933290‑933484
                                                                                                 |                                                                                                 
cTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCt                                                                                                 >  2:2850787/1‑100 (MQ=255)
cTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCt                                                                                                 <  1:2946230/100‑1 (MQ=255)
 tGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCt                                                                                                 >  1:1439256/1‑99 (MQ=255)
   gAGGCGTTGCTGATCCTTATTGTCCGGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGc                                                                                              <  2:1916623/100‑1 (MQ=255)
    agtcGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCa                                                                                             <  1:2609810/97‑1 (MQ=255)
    aGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTg                                                                                                >  1:1721214/1‑97 (MQ=255)
     ggCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCAt                                                                                            >  1:2000044/1‑100 (MQ=255)
     ggCGTTGCTGATCCTTATTATCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCAt                                                                                            >  2:613719/1‑100 (MQ=255)
     cgatctGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCAt                                                                                            <  2:1065347/95‑1 (MQ=255)
       cGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGa                                                                                          >  1:561468/1‑100 (MQ=255)
       cGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGa                                                                                          >  1:867703/1‑100 (MQ=255)
        tccgatcttCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGaa                                                                                         <  1:2813759/92‑1 (MQ=255)
           gCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCAtagatcg                                                                                      >  1:1065354/1‑94 (MQ=255)
           gCTGATCCTTATTGTCCTGTTTGCCGTCTGGTGGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAAcc                                                                                       <  2:193322/99‑1 (MQ=255)
             tGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTAt                                                                                    >  1:1604969/1‑100 (MQ=255)
             tGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTAt                                                                                    <  1:1470021/100‑1 (MQ=255)
              gATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTACCCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATc                                                                                   >  1:2097624/1‑100 (MQ=255)
              gATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTa                                                                                     >  1:1153751/1‑98 (MQ=255)
              gATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTAt                                                                                    >  2:2594007/1‑99 (MQ=255)
               aTCCTTATTGTCCTGTTTGCCGTCTTGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATc                                                                                   <  1:2869970/99‑1 (MQ=255)
               aTCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGATGCCTTACTAGGCTTTAACCCTTCGGACCCCAGATGGTAGCACACGGTCTGGCATGAAACtct                                                                                    >  2:2530272/1‑96 (MQ=255)
                tCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGaaagatcgga                                                                                 >  2:2813727/1‑92 (MQ=255)
                tCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCa                                                                                 <  2:1697008/100‑1 (MQ=255)
                tCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCa                                                                                 <  2:2637648/100‑1 (MQ=255)
                 ccTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCa                                                                                 <  2:1111511/99‑1 (MQ=255)
                   ttATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATaa                                                                              <  1:1336004/100‑1 (MQ=255)
                      ttGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAAttt                                                                           <  2:2817170/100‑1 (MQ=255)
                        gTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAg                                                                         >  2:2373518/1‑100 (MQ=255)
                         tCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAgg                                                                        <  1:68687/100‑1 (MQ=255)
                         tCCTGTTTGCCGTCTGGTTGATGGCTAACTTACTAAGCTTTAACCCTTCGGAACCCAGCTGGTAGCAAACGGTCTGGCATGAACCTATCCATAATTTaag                                                                        >  2:1916693/1‑98 (MQ=255)
                           cTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTg                                                                      >  1:2435763/1‑100 (MQ=255)
                            tGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTgg                                                                     <  1:1284171/100‑1 (MQ=255)
                             gTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCACAGCTGGTCGCAAACGATCTAGCATGAACCTATCCATAAtt                                                                            >  2:1737304/1‑92 (MQ=255)
                                tGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATg                                                                 >  2:399054/1‑100 (MQ=255)
                                tGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTGAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGAATGAACCTATCCATAATTTAGGTGGGATg                                                                 >  2:1824107/1‑100 (MQ=255)
                                  ccGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGcc                                                               >  2:1712688/1‑100 (MQ=255)
                                       tGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTg                                                          <  2:12957/100‑1 (MQ=255)
                                        ggTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGc                                                         >  2:1660030/1‑100 (MQ=255)
                                        ggTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGc                                                         >  1:989476/1‑100 (MQ=255)
                                        ggTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGc                                                         >  1:2411762/1‑100 (MQ=255)
                                         gTTGATGGCTGCCTTACTATGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCg                                                        <  1:1045335/100‑1 (MQ=255)
                                          ttGATGGCTGCCTTACTAAGCTTTCACCCTTCGGACCCCAGCTGGTCGCAAACAGCCTGGCATGAAACTAACCATAATATAGGTAGGATGCCCGGTGCGt                                                       >  1:96437/1‑100 (MQ=255)
                                           ttaTGGCTGCCTTACTAAGCTTTAACCCTTAGGACCCCAGCTGGTCGCAAACGGTCTGGCATGATCCTATCCATAATTTAGGTGGGATGCCCGGTGc                                                         <  2:966859/95‑1 (MQ=255)
                                           tGATGGCTGCCTTACTAAGCTTTAACCCTTCGTACCCCAGCTGTTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTg                                                      <  1:2450216/100‑1 (MQ=255)
                                           tGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTg                                                      >  2:1518404/1‑100 (MQ=255)
                                           tGATGGCTGCCGTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTg                                                      <  2:2878409/100‑1 (MQ=255)
                                             aTGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGt                                                    >  2:2832235/1‑100 (MQ=255)
                                               ggCTGCCTTACTAAGCTTTAACCCTTTGGACCCCAGCTGGTCGCAAACGGTCGGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTg                                                  <  1:1461597/100‑1 (MQ=255)
                                               ggCTGCCTTACTAAGCTTTAACCCTTCTGACCCCAGCTTGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTg                                                  <  1:381093/100‑1 (MQ=255)
                                               ggCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTg                                                  >  2:1537341/1‑100 (MQ=255)
                                               ggCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGAATGAAAATATACAAAATTTAGGTGGGATGAAAGGTGAGTGGtt                                                   >  1:201784/1‑99 (MQ=255)
                                                gCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTgg                                                 <  1:1407568/100‑1 (MQ=255)
                                                gCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTgg                                                 <  1:2502486/100‑1 (MQ=255)
                                                 cTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGc                                                <  2:1232187/100‑1 (MQ=255)
                                                 cTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGc                                                <  2:2523016/100‑1 (MQ=255)
                                                 cTGCCTTACTAAGCTTTAACCCTTCGGACCCAAGCTGGTCGCCAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGt                                                    <  2:1965572/96‑1 (MQ=255)
                                                  tGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCa                                               <  1:812198/100‑1 (MQ=255)
                                                  tGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGCCATGAACCTATCAAAAATTAAGATGAGATACCCTGTGCCTGGTTgg                                                 >  1:1944279/1‑98 (MQ=255)
                                                     cTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGAt                                            >  1:219308/1‑100 (MQ=255)
                                                       tACTAAGCTTTAACCCTTCGAACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATAc                                          >  1:72493/1‑100 (MQ=255)
                                                        aCTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACg                                         >  2:2638245/1‑100 (MQ=255)
                                                         cTAAGCTTTAACCCTTCGGACCTCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGc                                        <  1:2347775/100‑1 (MQ=255)
                                                           aaGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTg                                      >  2:1922746/1‑100 (MQ=255)
                                                           aaGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTg                                      >  2:1669452/1‑100 (MQ=255)
                                                             gCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGtt                                    >  1:68092/1‑100 (MQ=255)
                                                              cTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGAGGTTGGCAGATACGCTGTTc                                   >  1:1441122/1‑100 (MQ=255)
                                                               tttAACCCTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCt                                  <  2:162452/100‑1 (MQ=255)
                                                                   aCCCTTCGGACCCCAGCTGGTCGCAAATGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGTTTGGCAGATACGCTGTTCTTTa                               <  2:2842947/99‑1 (MQ=255)
                                                                     ccTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAttt                            >  2:137643/1‑100 (MQ=255)
                                                                     ccTTCGGACCCCAGCTGGTCGCAAACGGTCTGACATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAttt                            >  1:2889065/1‑100 (MQ=255)
                                                                      cTTCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGTACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTAtttt                           <  1:1667909/100‑1 (MQ=255)
                                                                        tCGGACCCCAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTg                         <  1:1891815/100‑1 (MQ=255)
                                                                        cgatctcccAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTg                         <  2:1885884/94‑1 (MQ=255)
                                                                             ccccAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTg                    >  2:2362455/1‑100 (MQ=255)
                                                                              cccAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTgagatcg                   >  1:1885906/1‑94 (MQ=255)
                                                                              cccAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGa                   <  1:2118489/100‑1 (MQ=255)
                                                                               ccAGCTGGTCGCAAACGGTCTGGCATGAACCTATTCATAATTTTGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGAt                  <  1:2360369/100‑1 (MQ=255)
                                                                               ccAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGAt                  >  1:390575/1‑100 (MQ=255)
                                                                               ccAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATAAGCTGTTCTTTATTTTTGGCGTGAt                  >  2:325487/1‑100 (MQ=255)
                                                                               ccAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGATTGGCAGATACGCTGTTCTTTATTTTTGGCGTGAt                  >  1:841142/1‑100 (MQ=255)
                                                                                cAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATg                 >  2:2181418/1‑100 (MQ=255)
                                                                                cAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATg                 <  1:1824128/100‑1 (MQ=255)
                                                                                cAGCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATg                 <  2:2942501/100‑1 (MQ=255)
                                                                                  gCTGGTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGc               <  2:2339583/100‑1 (MQ=255)
                                                                                     ggTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCTCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTa            <  1:415908/100‑1 (MQ=255)
                                                                                      ttCGCAAACGGTCTTTCATTAACCTATCCATAATTTATGTTTGATTCTCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTac           <  1:2602134/99‑1 (MQ=255)
                                                                                      gTCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTac           >  2:2602830/1‑100 (MQ=255)
                                                                                       tCGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTaca          <  1:2373545/100‑1 (MQ=255)
                                                                                        cGCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTacac         >  1:2076697/1‑100 (MQ=255)
                                                                                         gCAAACGGTCTGGCATGAACCTATCTATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACAcc        <  1:1227250/100‑1 (MQ=255)
                                                                                         gCAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTaca          >  1:497295/1‑98 (MQ=255)
                                                                                          cAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACAcc        >  2:1587849/1‑99 (MQ=255)
                                                                                          cAAACGGTCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCa       >  1:2413111/1‑100 (MQ=255)
                                                                                               ggCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTccc  <  2:96436/100‑1 (MQ=255)
                                                                                                 |                                                                                                 
CTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCC  >  NC_000913/933290‑933484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: