Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 975,083 T→A 100% D255E (GAT→GAA mukF → chromosome condensin MukBEF, kleisin‑like subunit, binds calcium

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913975,0830TA97.2% 391.0 / ‑1.0 119D255E (GAT→GAAmukFchromosome condensin MukBEF, kleisin‑like subunit, binds calcium
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (57/58);  total (60/59)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

AAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACC  >  NC_000913/974986‑975179
                                                                                                 |                                                                                                
aaGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACt                                                                                                <  2:3016956/100‑1 (MQ=255)
 aGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTg                                                                                               <  2:1510196/100‑1 (MQ=255)
   cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCa                                                                                             >  1:2559659/1‑100 (MQ=255)
      cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCagag                                                                                          <  2:825706/100‑1 (MQ=255)
      cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCagag                                                                                          <  2:1804968/100‑1 (MQ=255)
        ggCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCa                                                                                        >  2:2367310/1‑100 (MQ=255)
        cgCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCa                                                                                        >  2:1414469/2‑100 (MQ=255)
          cGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGAACGTCTGGTGTTCGATCTGCAGAGCaaa                                                                                      >  1:777222/1‑100 (MQ=255)
               aaTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGAGGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGa                                                                                 >  1:2139645/1‑100 (MQ=255)
                aTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGAt                                                                                >  2:2791869/1‑100 (MQ=255)
                aTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGAt                                                                                <  2:2445764/100‑1 (MQ=255)
                 ttGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATc                                                                               >  2:133548/1‑100 (MQ=255)
                  tGCATGTTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGAAGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCg                                                                              <  1:2013271/100‑1 (MQ=255)
                   gCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGt                                                                             >  2:2389118/1‑100 (MQ=255)
                   gCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGAATTTCGTCGATCGTCTGGTATTAGAACTGCAGAGCAAACTCGATCg                                                                              >  1:284640/1‑99 (MQ=255)
                    cAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGta                                                                            >  1:2394519/1‑100 (MQ=255)
                     aGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGATGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtat                                                                           <  1:825772/100‑1 (MQ=255)
                     aGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtat                                                                           <  1:2887128/100‑1 (MQ=255)
                     aGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtat                                                                           >  1:2642963/1‑100 (MQ=255)
                     aGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtat                                                                           <  1:2413232/100‑1 (MQ=255)
                      ggCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatt                                                                          >  1:2094901/1‑100 (MQ=255)
                      ggCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatt                                                                          <  2:2496097/100‑1 (MQ=255)
                       gCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatta                                                                         <  1:277806/100‑1 (MQ=255)
                        cTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGGGCAAACTCGATCGtattat                                                                        >  2:2231638/1‑100 (MQ=255)
                        cTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtattat                                                                        <  1:1140148/100‑1 (MQ=255)
                        cTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtattat                                                                        <  1:1926928/100‑1 (MQ=255)
                         tAATCTGTTGCGCATTCAGGATGCGATTATGACCTATGATGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATc                                                                       <  1:524300/100‑1 (MQ=255)
                         tAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATc                                                                       >  2:2520516/1‑100 (MQ=255)
                         tAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATc                                                                       <  1:487648/100‑1 (MQ=255)
                          aaTCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCa                                                                      <  1:1620987/100‑1 (MQ=255)
                          aaTCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCa                                                                      <  1:1654224/100‑1 (MQ=255)
                          aaTCTGTTGCGCAGTCAGGATGCGACGATGACCCATGAAGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTACCa                                                                      >  2:1871672/1‑100 (MQ=255)
                          aaTCTGTTGCGCAGTCAAGATAAGACGATGACCAATGAAGATCTGCATTTCGTCGATCGTCTGGGGTTCGAACTGCAGAGCAAACTCGAGCGTATTATCa                                                                      >  2:1871512/1‑100 (MQ=255)
                           aTCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAg                                                                     >  1:5544/1‑100 (MQ=255)
                             cTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGtt                                                                   <  2:18947/100‑1 (MQ=255)
                              tGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTg                                                                  <  2:1433771/100‑1 (MQ=255)
                                ttGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTggg                                                                <  2:2094878/100‑1 (MQ=255)
                                 tGCGCATTTAGGATGCGACGATGACCTATGACGATCTGCTTTTAGTCGATAGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                               <  1:1498024/100‑1 (MQ=255)
                                 tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                               >  2:1114333/1‑100 (MQ=255)
                                 tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                               <  2:103890/100‑1 (MQ=255)
                                  gcgcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGc                                                              <  1:2990257/100‑1 (MQ=255)
                                    gcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATGGTCTGGTGTTCGAACTGCAGAGAAAACTCGATAGTCTAATTAGTTgagaca                                                             >  2:1139480/1‑94 (MQ=255)
                                     cATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAg                                                           >  2:1884607/1‑100 (MQ=255)
                                     cATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAg                                                           <  1:2593769/100‑1 (MQ=255)
                                     cATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCAATCGTATTATCAGTTGGGGcc                                                             >  1:1427415/1‑98 (MQ=255)
                                      aTTTAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCAAACTGCAGAGCAAACTCGATCGTATTATTAGTTGGGGCCacc                                                          <  1:2047203/100‑3 (MQ=255)
                                        tCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCaa                                                        <  1:1879757/100‑1 (MQ=255)
                                         cAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAAt                                                       >  2:1958694/1‑100 (MQ=255)
                                          aGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATc                                                      <  1:2819129/100‑1 (MQ=255)
                                           ggATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCaa                                                        >  1:1809249/1‑97 (MQ=255)
                                             aTGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTAGGGGCCAGCAATCCAt                                                   >  1:181404/1‑100 (MQ=255)
                                              tGCGACGATGATCCATTACGTTCTGTATTTTGTCGATTGTTTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATc                                                  <  1:2908696/100‑1 (MQ=255)
                                              tGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATc                                                  <  2:389649/100‑1 (MQ=255)
                                               gCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCg                                                 <  2:632307/100‑1 (MQ=255)
                                               gCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCg                                                 <  1:529954/100‑1 (MQ=255)
                                                cgacgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGACCAGCAATCCATCGa                                                >  1:978365/1‑100 (MQ=255)
                                                   cgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACtt                                             >  2:1966748/1‑100 (MQ=255)
                                                     aTGACCCATGACGATCTGTATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtg                                            <  1:1396063/99‑1 (MQ=255)
                                                      tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgtg                                          <  2:3004415/100‑1 (MQ=255)
                                                       gACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTgg                                         <  1:1665706/100‑1 (MQ=255)
                                                         cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                       >  2:823801/1‑100 (MQ=255)
                                                         cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                       <  2:2786810/100‑1 (MQ=255)
                                                         cccATGACGATCTGCATTTCGTCGAACGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                       >  2:296529/1‑100 (MQ=255)
                                                         cccATGACGATCTACATTTCGTCGATCGTCTGGTGTTCAAACTGGAGAGCAAACTCGATCGTATTATCAGATGGGGGCCGCAATCCATCGACTTGTGGAt                                       >  2:1875387/1‑100 (MQ=255)
                                                          ccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAACAAACTCGATCGTATTATCAGTTGTTGCCAGAAATAAATCGACTAGTGGAtt                                      >  1:2864669/1‑100 (MQ=255)
                                                          ccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTgg                                         <  1:2250676/97‑1 (MQ=255)
                                                          ccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAtt                                      >  1:1392561/1‑100 (MQ=255)
                                                          ccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAtt                                      >  2:2000398/1‑100 (MQ=255)
                                                          ccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAtt                                      <  2:553621/100‑1 (MQ=255)
                                                            aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTg                                     <  1:3039319/99‑1 (MQ=255)
                                                             tGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGc                                   <  1:1114343/100‑1 (MQ=255)
                                                              gACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCt                                  <  2:2307328/100‑1 (MQ=255)
                                                                 gATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACg                               <  1:1811758/100‑1 (MQ=255)
                                                                  aTCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGa                              >  2:1779773/1‑100 (MQ=255)
                                                                  aTCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGa                              >  2:2608892/1‑100 (MQ=255)
                                                                    cTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGAcc                            >  2:1932136/1‑100 (MQ=255)
                                                                     tGCATTTCGTCGATCGTCTGGTGTTCGAACTGTAGAGCAAACTTGTTCGTATTATCAGTTGGGGCCAGCAATCCAGCGACTTGTGGATTGGCTACGACCg                           <  1:2288684/100‑1 (MQ=255)
                                                                       cATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGcc                         <  1:1751200/100‑1 (MQ=255)
                                                                       cATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGcc                         <  1:2223935/100‑1 (MQ=255)
                                                                       cATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGAAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGcc                         >  2:1384219/1‑100 (MQ=255)
                                                                        aTTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCa                        >  1:1249280/1‑100 (MQ=255)
                                                                            cgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTa                    >  2:1409560/1‑100 (MQ=255)
                                                                             gtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATTAGTTGGGGTCAGCAATTCATCGAGTTGTAGATTGGCTACGATCACCAAGTa                    >  1:346291/1‑99 (MQ=255)
                                                                                   cGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAt             <  1:490757/100‑1 (MQ=255)
                                                                                    gTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAtt            >  1:704393/1‑100 (MQ=255)
                                                                                    gTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAtt            <  2:1991087/100‑1 (MQ=255)
                                                                                    gTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAtt            >  1:1967384/1‑100 (MQ=255)
                                                                                     tCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAtt            >  2:14922/1‑99 (MQ=255)
                                                                                      cTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTa          >  1:407685/1‑100 (MQ=255)
                                                                                      cTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTa          >  2:145791/1‑100 (MQ=255)
                                                                                       tGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAt         >  2:1418557/1‑100 (MQ=255)
                                                                                       tGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAt         <  1:304341/100‑1 (MQ=255)
                                                                                       tGGTGTTCGAACTGCAGAGCAAACACGATCGTATTATCAGTTGGTGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTa          >  2:653466/1‑99 (MQ=255)
                                                                                        ggTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTTGATTGGTTAAGACCGCCACGTACACAAATTTAtt        >  2:303124/1‑100 (MQ=255)
                                                                                        ggTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAtt        >  1:489316/1‑100 (MQ=255)
                                                                                        ggTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAtt        <  1:1845337/100‑1 (MQ=255)
                                                                                          tgtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCg      <  1:706086/100‑1 (MQ=255)
                                                                                             tCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTAc   <  1:1884629/100‑1 (MQ=255)
                                                                                             tCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGAACGCAACGTACACAAATTTATTCGTAc   >  1:1553684/1‑100 (MQ=255)
                                                                                              cGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTAcc  >  2:1085173/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACC  >  NC_000913/974986‑975179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: