Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,036,094 A→G 15.2% I109V (ATC→GTC)  hyaD → hydrogenase 1 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,036,0940AG15.2% 265.4 / 40.7 120I109V (ATC→GTC) hyaDhydrogenase 1 maturation protease
Reads supporting (aligned to +/- strand):  ref base A (51/49);  new base G (13/5);  total (65/55)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01

Only 100 of 129 total aligned reads displayed.

CTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAAC  >  NC_000913/1035995‑1036193
                                                                                                   |                                                                                                   
cTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGata                                                                                                     >  1:2463525/1‑100 (MQ=255)
cTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGata                                                                                                     >  2:39740/1‑100 (MQ=255)
cTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGata                                                                                                     >  1:1570815/1‑100 (MQ=255)
cTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGata                                                                                                     >  1:1603065/1‑100 (MQ=255)
 tGCGAACCTATGCCGGAGAACGCATTCCTGCTTATTTTTGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGatat                                                                                                    <  1:57711/100‑1 (MQ=255)
  gCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATc                                                                                                   <  1:2641115/100‑1 (MQ=255)
   atctccTATGCCGTAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATcc                                                                                                  <  1:1760341/96‑1 (MQ=255)
       ccTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCgg                                                                                              <  2:1202105/100‑1 (MQ=255)
       ccTATGCCGGAGAACGCATTCCAGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATccagat                                                                                              >  2:1760321/1‑96 (MQ=255)
        cTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGa                                                                                             <  1:2742449/100‑1 (MQ=255)
           tGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACAt                                                                                          >  2:2146910/1‑100 (MQ=255)
             ccGGAGAACGCATTCCGGCTTATCTCAGCGGGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCt                                                                                        >  2:78431/1‑100 (MQ=255)
             ccGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCt                                                                                        >  1:2150444/1‑100 (MQ=255)
              cGGAGAACGCATTCCGGCTTATCTCATCGCGAAGAAAATGAGCTTGCATCAGAACAGTTTCTCCGAAGTGTTTGCGCTGGCGGATATCCGCGGACATCTg                                                                                       <  2:1311631/100‑1 (MQ=255)
              cGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTg                                                                                       <  1:106842/100‑1 (MQ=255)
              cGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTg                                                                                       <  2:2934427/100‑1 (MQ=255)
                gagaACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGcc                                                                                     <  2:2055132/100‑1 (MQ=255)
                gagaACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGcc                                                                                     >  1:2724884/1‑100 (MQ=255)
                gagaACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCAGATGTCCGCGGATATCCGcc                                                                                     <  2:2727330/100‑1 (MQ=39)
                 agaACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCa                                                                                    <  1:2768819/100‑1 (MQ=255)
                  gagcGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGcct                                                                                    <  2:1417335/96‑2 (MQ=255)
                   aaCGCATTCCGGCTTATCTCTGCGCTAATAATATGATTCTTCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGc                                                                                  <  1:762401/100‑1 (MQ=255)
                   aaCGCATTCCGGCTTATATCATTGCGAAGAAATTGTGCGTGCATCAGAACAGTTTCGCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATc                                                                                         <  1:142490/93‑1 (MQ=255)
                    aCGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTACCAGca                                                                                 >  1:628479/1‑100 (MQ=255)
                    aCGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCAGATGTCCGCGGATATCCGCCAGcg                                                                                 <  1:2433246/100‑2 (MQ=38)
                    aCGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGACGCTGGCGGATATCCGCGGACATCTGCCAGca                                                                                 >  1:1133784/1‑100 (MQ=255)
                     cGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGcac                                                                                <  2:1405051/100‑1 (MQ=255)
                     cGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGcac                                                                                >  1:1464334/1‑100 (MQ=255)
                     cGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCAGATGTCCGCGGATATCCGCCAGcgc                                                                                <  2:2216921/100‑3 (MQ=37)
                      gCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGcaca                                                                               <  2:623294/100‑1 (MQ=255)
                       cATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACat                                                                              >  1:2177803/1‑100 (MQ=255)
                       cATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACat                                                                              >  1:1753957/1‑100 (MQ=255)
                       cATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCAGATGTCCGCGGATATCCGCCAGcgcca                                                                              <  1:1762126/100‑5 (MQ=37)
                        aTTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGGAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACata                                                                             <  1:1079239/100‑1 (MQ=255)
                           ccGGTTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAATAGTTTCTCCGAAGTGTTGGCGCTGGCAGATGTCCGCGGATATCCGCCAGcgccaacac                                                                          <  1:1227403/100‑9 (MQ=21)
                           ccGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATAtt                                                                           >  1:2574912/1‑99 (MQ=255)
                           ccGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTg                                                                          <  1:2910688/100‑1 (MQ=255)
                             ggCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTgt                                                                         >  2:716780/1‑98 (MQ=255)
                               nttATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTACCCt                                                                      >  2:1359774/2‑100 (MQ=255)
                                  aTCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCtcgt                                                                   >  2:1191562/1‑100 (MQ=255)
                                   tctcATCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCtcgtc                                                                  <  2:2602409/100‑1 (MQ=255)
                                   tctcAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCtcgt                                                                   >  1:2363032/1‑99 (MQ=255)
                                    ctcAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCtcgtcg                                                                 <  2:1124772/100‑1 (MQ=255)
                                    ctcAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGACGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCtcgtcg                                                                 >  1:109057/1‑100 (MQ=255)
                                       aGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTc                                                              <  1:1537355/100‑1 (MQ=255)
                                       aGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTc                                                              >  2:879061/1‑100 (MQ=255)
                                         cgcgAAGAAAATAAGCCTGCATCAGAACAGTTTCGACGAAGTGTTGGCGCTGGCGGAAAACCGCGGACATCTGCCAGCACATATTGCGCgcgtacgt                                                               >  2:2136095/1‑93 (MQ=255)
                                          gcgAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTc                                                              <  2:558907/97‑1 (MQ=255)
                                           cgAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCa                                                          >  2:2383770/1‑100 (MQ=255)
                                            gaagaaAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCaa                                                         >  1:371236/1‑100 (MQ=255)
                                            gaagaaAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCaa                                                         >  1:1493817/1‑100 (MQ=255)
                                              agaaAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGTACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCa                                                          <  1:2142153/97‑1 (MQ=255)
                                              agaaAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGAGAACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAAcc                                                       >  1:37411/1‑100 (MQ=255)
                                               gaaAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAAccc                                                      >  2:1186883/1‑100 (MQ=255)
                                                  aaTGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCa                                                   <  1:1901039/100‑1 (MQ=255)
                                                    tGAGCCTGCATCAGAACAGTTTTTTCGAAGTGTTGGCGCTTGCGGATATCCGCGGATATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAAt                                                 <  1:183480/100‑1 (MQ=255)
                                                       gCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGTATATCCGCGGATATCTGCCAGCACATATTGCCCTCGTTGGTCTGCAACCCGCAATGCt                                              <  2:477649/100‑1 (MQ=255)
                                                       gCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCt                                              >  1:607959/1‑100 (MQ=255)
                                                        ccTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTc                                             >  2:1676856/1‑100 (MQ=255)
                                                        ccTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTc                                             <  1:2383797/100‑1 (MQ=255)
                                                          tGCATCAGAACTGTTTCTCCGAAGTGTTGGCGCTTGCGGATATCCGCGGACATCTGCCAGCACATATTACACTCATCGGTCTGCAACCCGCAATGcacaa                                           >  2:2338003/1‑96 (MQ=255)
                                                          tGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcga                                           >  2:1701231/1‑100 (MQ=255)
                                                           gCATCAGAACAGTTTCTCCTATGTGTTGGCGCTGGTGGATATCCGCGGACATCTGCCAGCACATATGGCCCTCGTCGGTCTGCAACCCGCAATGCTcgac                                          <  1:538448/100‑1 (MQ=255)
                                                           gCATCAGAACAGTTTCTCCGAAGTGTTGGCGTTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcgac                                          <  1:406306/100‑1 (MQ=255)
                                                           gCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcgac                                          <  1:2664508/100‑1 (MQ=255)
                                                            cATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATCTCCGCGCACATCTCCCAGAACATATTACCCTCGTCAAACTACAACCCGCAATGCTcgacg                                         >  1:1109401/1‑100 (MQ=255)
                                                              tCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcgacg                                         <  1:1185966/98‑1 (MQ=255)
                                                                    cAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTAcggc                                 >  1:2239321/1‑100 (MQ=255)
                                                                      gTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCTCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTAcggcgg                               <  1:994430/100‑1 (MQ=255)
                                                                      gTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTAcggcgg                               >  2:2662626/1‑100 (MQ=255)
                                                                      gTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTAcggcgg                               <  1:1325482/100‑1 (MQ=255)
                                                                      gTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTAcggcgg                               >  1:1525869/1‑100 (MQ=255)
                                                                      gTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTAcggcag                               >  2:1838415/1‑98 (MQ=255)
                                                                       tttCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACAACTACGGCGGt                              >  1:275792/1‑100 (MQ=255)
                                                                        ttCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTa                             >  2:928676/1‑100 (MQ=255)
                                                                        ttCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATAATGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTa                             >  1:2508693/1‑100 (MQ=255)
                                                                            ccGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCACTGCTCGACGACTACGGCGGTAGCCt                         >  2:2690042/1‑100 (MQ=255)
                                                                            ccGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGcc                          >  1:647551/1‑99 (MQ=255)
                                                                            ccGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGATCTACAACCCGCAATGCTCGACGACTACGGCGGTAGCCt                         >  1:2346383/1‑100 (MQ=255)
                                                                             cGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTg                        <  2:1570797/100‑1 (MQ=255)
                                                                             cGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTg                        <  2:2643534/100‑1 (MQ=255)
                                                                              gAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTg                        >  2:626451/1‑99 (MQ=255)
                                                                                aGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGTTCGACGACTACGGCGGTAGCCTGAGc                     <  1:1849240/100‑1 (MQ=255)
                                                                                  aatatgtgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGa                   >  2:1694902/8‑100 (MQ=25)
                                                                                   gtTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGaa                  >  2:2198449/1‑100 (MQ=255)
                                                                                   gtTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGATCTGCAACCCGCAATACTCGACGACTACGACGGTAGCCTGAGCGaa                  >  1:755822/1‑100 (MQ=255)
                                                                                    tatgtgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGaa                  >  2:1928994/6‑99 (MQ=37)
                                                                                     atgtgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACt                >  2:2894216/5‑100 (MQ=37)
                                                                                     atgtgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACt                >  1:2931326/5‑100 (MQ=37)
                                                                                     atgtgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACt                >  1:2931226/5‑100 (MQ=37)
                                                                                     atgtgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACt                >  2:1607582/5‑100 (MQ=37)
                                                                                      ggCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCACAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTg               >  1:320379/1‑100 (MQ=255)
                                                                                       gtgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGATAGACGACTACGGCGGTAGCCTGAGCGAACTgg              >  1:2381660/3‑100 (MQ=25)
                                                                                        tgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACt                >  1:889218/2‑97 (MQ=37)
                                                                                        tgcTGGCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGc             >  2:620757/2‑100 (MQ=38)
                                                                                           tGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACg          >  1:219901/1‑100 (MQ=255)
                                                                                            ggCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACgg         >  1:2821524/1‑100 (MQ=255)
                                                                                            ggCAGATGTCCGCGGATATCCGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACgg         >  2:2019333/1‑100 (MQ=38)
                                                                                                   atCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAAc  >  2:2557742/1‑100 (MQ=255)
                                                                                                   atCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAAc  >  1:282329/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAAC  >  NC_000913/1035995‑1036193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: