Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,100,233 G→A 100% intergenic (+39/‑63) ycdY → / → ycdZ redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,100,2330GA99.1% 356.2 / ‑4.5 106intergenic (+39/‑63)ycdY/ycdZredox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (50/55);  total (51/55)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 119 total aligned reads displayed.

CTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACA  >  NC_000913/1100135‑1100328
                                                                                                  |                                                                                               
cTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgt                                                                                                <  2:2594667/100‑1 (MQ=255)
 tAACCCGCGATGCCATTAGTGCAATGTGGGATGAGCTGTAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttatt                                                                                               <  1:2413667/100‑1 (MQ=255)
 tAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttatt                                                                                               <  1:2441144/100‑1 (MQ=255)
  aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                              <  2:527068/100‑1 (MQ=255)
  aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                              <  1:978845/100‑1 (MQ=255)
   aCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgt                                                                                             <  2:1599492/100‑1 (MQ=255)
   aCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGCGGTCTACGTCACTCATAACTGCAACGGATAATttattgt                                                                                             >  2:2134065/1‑100 (MQ=255)
     ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                           <  1:1507731/100‑1 (MQ=255)
     ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                           <  1:1875336/100‑1 (MQ=255)
     cagcgATGCCATTAGTGCAATGTGGGAAGAGCTGGAAGAAGATTCTGAAGAATAACTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                           >  2:881075/3‑100 (MQ=255)
      cgcgATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                           >  1:1502559/1‑99 (MQ=255)
            gCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATaaaa                                                                                    >  1:1567654/1‑100 (MQ=255)
            gCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATaaaa                                                                                    >  2:3032443/1‑100 (MQ=255)
              cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                  >  2:1526613/1‑100 (MQ=255)
              cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                  <  2:2368721/100‑1 (MQ=255)
              cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAAAGGATAATTTATTGTTGCATAAAAtg                                                                                  >  1:1339264/1‑100 (MQ=255)
                ttAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGCAGAATAAGTGTGGTCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg                                                                                <  1:2134088/100‑1 (MQ=255)
                ttAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg                                                                                <  1:955032/100‑1 (MQ=255)
                ttAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg                                                                                <  2:2017276/100‑1 (MQ=255)
                ttAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtg                                                                                <  1:25376/100‑1 (MQ=255)
                   gTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGc                                                                             >  1:1748690/1‑100 (MQ=255)
                   gTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGc                                                                             >  1:2103955/1‑100 (MQ=255)
                    tGCAATGATGGACGAGCTGGAAGAAGATGCTGTAAAATAAGTCTGATGTGCGTCACTGATCACTATTTCGAGTAATTTATTGTTTCAGAAAAGGAGTGCt                                                                            <  2:1192717/100‑1 (MQ=255)
                         tgtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtc                                                                       <  2:761917/100‑1 (MQ=255)
                         tgtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtc                                                                       <  1:788160/100‑1 (MQ=255)
                          gtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtct                                                                      >  1:1736284/1‑100 (MQ=255)
                           tgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtctc                                                                     >  2:307681/1‑100 (MQ=255)
                           tgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtctc                                                                     >  1:2226918/1‑100 (MQ=255)
                            gggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtca                                                                    >  1:2432764/1‑100 (MQ=255)
                             ggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcat                                                                   >  2:508666/1‑100 (MQ=255)
                              gACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcatt                                                                  >  1:359795/1‑100 (MQ=255)
                              gACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcatt                                                                  >  1:2459825/1‑100 (MQ=255)
                                cGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcattca                                                                <  1:2001735/100‑1 (MQ=255)
                                  aGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATg                                                              <  2:2278901/100‑1 (MQ=255)
                                   gCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATgg                                                             <  1:508669/100‑1 (MQ=255)
                                    cTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGc                                                            >  2:332010/1‑100 (MQ=255)
                                      ggAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGcc                                                           >  2:1049940/1‑99 (MQ=255)
                                      ggAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACAGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcg                                                          >  1:2440377/1‑100 (MQ=255)
                                           aagaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGttt                                                     <  2:2432735/100‑1 (MQ=255)
                                           aagaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtt                                                      <  1:685174/99‑1 (MQ=255)
                                            agaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtttt                                                    >  2:1627254/1‑100 (MQ=255)
                                             gaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTc                                                   <  2:553381/100‑1 (MQ=255)
                                             gaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTc                                                   <  1:1028801/100‑1 (MQ=255)
                                                 cTGAAGCATAAGTGTCATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAACTCATTCATGGCCGCGTTTTCTGCt                                               >  2:1408770/1‑100 (MQ=255)
                                                 cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCt                                               >  1:3431/1‑100 (MQ=255)
                                                 cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCt                                               >  1:462314/1‑100 (MQ=255)
                                                  tGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTa                                              <  1:1677826/100‑1 (MQ=255)
                                                  tGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTa                                              >  1:903202/1‑100 (MQ=255)
                                                  tGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTa                                              >  2:782728/1‑100 (MQ=255)
                                                    aagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATc                                            >  1:2925304/1‑100 (MQ=255)
                                                     agaaTAAATGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCa                                           >  2:1581993/1‑100 (MQ=255)
                                                      gaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTTGCTCTCATTCATGGTCGCGTTTTCTGCTATCa                                           <  2:593220/99‑1 (MQ=255)
                                                      gaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCAt                                          <  1:176433/100‑1 (MQ=255)
                                                      gaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCAt                                          >  1:2241965/1‑100 (MQ=255)
                                                      gaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCAt                                          <  2:1136325/100‑1 (MQ=255)
                                                        aTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgc                                        <  1:190817/100‑1 (MQ=255)
                                                         tAAGTGTTATCTATGTCACTCATATCTGCAACGTATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcg                                       <  1:1523278/100‑1 (MQ=255)
                                                         tAAGTGTGATCTACGTCACTCATATCTGTAACGGTTAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcg                                       <  1:1283008/100‑1 (MQ=255)
                                                         tAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcg                                       <  2:2932463/100‑1 (MQ=255)
                                                         tAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcg                                       <  2:1735890/100‑1 (MQ=255)
                                                          aaGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGAATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTTTCATgcgc                                      >  2:3000262/1‑100 (MQ=255)
                                                           aGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTa                                              >  2:2376130/1‑91 (MQ=255)
                                                           aGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgaatg                                     >  1:2932652/1‑96 (MQ=255)
                                                            gtgtGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgccgcg                                    <  2:235452/100‑5 (MQ=255)
                                                             tgtgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGc                                   <  1:2743191/100‑1 (MQ=255)
                                                              gtgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCa                                  <  1:2737917/100‑1 (MQ=255)
                                                              gtgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCa                                  >  2:2106183/1‑100 (MQ=255)
                                                               tgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGAACGCGTTTTCTGCTATCATGCGCGGCAt                                 >  1:1438834/1‑100 (MQ=255)
                                                                gATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGTATAAAATGTGTGCTCGATCTCAGTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                                <  2:2615289/100‑1 (MQ=255)
                                                                gATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                                <  2:1073565/100‑1 (MQ=255)
                                                                 aTCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGa                               <  1:62992/100‑1 (MQ=255)
                                                                  tCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGaa                              <  1:2157271/100‑1 (MQ=255)
                                                                  tCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGaa                              <  1:3040438/100‑1 (MQ=255)
                                                                  tCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGaa                              <  2:1632367/100‑1 (MQ=255)
                                                                   cTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAGCATGCGCGGCATGAAc                             >  2:1769643/1‑100 (MQ=255)
                                                                     aCGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATAATGAGCGGCATGaaaa                            >  1:2847140/1‑97 (MQ=255)
                                                                      cGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATa                          >  2:274821/1‑100 (MQ=255)
                                                                       gTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATa                          >  2:562040/1‑99 (MQ=255)
                                                                        tCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACt                        <  1:1980576/100‑1 (MQ=255)
                                                                          aCTCATAACTGCAACGGATAATTTATTGTTGCATAATATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtc                      <  1:913604/100‑1 (MQ=255)
                                                                          aCTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtc                      <  2:1967758/100‑1 (MQ=255)
                                                                          aCTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATAACCACGATTTCTGCTATCATGCGCGGCATGAACATACTtc                      >  1:1355139/1‑100 (MQ=255)
                                                                           cTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtct                     >  2:2074092/1‑100 (MQ=255)
                                                                            tCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctc                    >  2:498505/1‑100 (MQ=255)
                                                                             cATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctct                   >  1:1263958/1‑100 (MQ=255)
                                                                             cATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATAGCCGCGTATTCTGCTATAATGCGCGGCATGAACATACTtctct                   >  1:1468005/1‑100 (MQ=255)
                                                                              aTAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctctc                  <  1:932148/100‑1 (MQ=255)
                                                                              aTAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctctc                  <  1:1927732/100‑1 (MQ=255)
                                                                              aTAACTGCAACAGATAATTTGCCGTTGCATAAAATGTGTGCTCGATCTCATTCATGGACGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctctc                  >  2:676236/1‑100 (MQ=255)
                                                                               tAACTGCAACGGATAATTTATTGTTGCATTAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCGGCTATCATGCGCGGCATGAACATACTTctctct                 <  2:1819537/100‑1 (MQ=255)
                                                                                  tccgatctgATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAtt              <  1:2745037/92‑1 (MQ=255)
                                                                                   tGCAGCGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCTCGTTTTCTGCTATCATGCGCGGCATGAACATACTGCGCTCTATTg             <  2:2528354/100‑1 (MQ=255)
                                                                                   tGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTg             <  2:2006314/100‑1 (MQ=255)
                                                                                    gCAACGGATAATTTATTGTTGCATAAAATGTTTGCTTGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGc            <  1:1006183/100‑1 (MQ=255)
                                                                                    gCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGc            <  1:1893286/100‑1 (MQ=255)
                                                                                      aaCGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCaa          <  2:1749050/100‑1 (MQ=255)
                                                                                          gATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAttagatcgga      >  2:2745005/1‑92 (MQ=255)
                                                                                              aTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaaca  >  1:2592981/1‑100 (MQ=255)
                                                                                              aTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaaca  <  2:1711019/100‑1 (MQ=255)
                                                                                              aTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaaca  >  2:2772335/1‑100 (MQ=255)
                                                                                                  |                                                                                               
CTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACA  >  NC_000913/1100135‑1100328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: