Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 129,360 G→T 100% intergenic (+24/+47) lpd → / ← yacH dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913129,3600GT100.0% 403.6 / ‑6.7 129intergenic (+24/+47)lpd/yacHdihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base T (53/72);  minor base A (2/0);  total (57/72)

Only 100 of 129 total aligned reads displayed.

CTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTG  >  NC_000913/129263‑129456
                                                                                                 |                                                                                                
  gtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAt                                                                                              <  2:2209412/100‑1 (MQ=255)
   tgGGCCTGGCGGCAGAAGTTTTCGAAGGTAGCATTACCGACCTGACGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAtt                                                                                             <  1:1827381/100‑1 (MQ=255)
    gggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTa                                                                                            >  2:869946/1‑100 (MQ=255)
     ggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaa                                                                                           >  2:2429802/1‑100 (MQ=255)
       ccTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaa                                                                                         <  2:1293936/100‑1 (MQ=255)
       ccTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaa                                                                                         <  1:1152168/100‑1 (MQ=255)
           gcggcAGAAGTGTTCGAAGGTAGCATTACCGACCTTTCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGc                                                                                     <  1:2541227/100‑1 (MQ=255)
           gcggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGc                                                                                     <  1:103082/100‑1 (MQ=255)
            cggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCg                                                                                    >  1:1847500/1‑100 (MQ=255)
            cggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCg                                                                                    >  2:302441/1‑100 (MQ=255)
             ggcAGAAGTGTTCAAAGGTAGCATGTCCGACCTGCCGAACCCAAAAGCGAAGAAGAATGAATTTTTCCTTTTCCGCAACTTCCCTCAATTAAAAAAGc                                                                                     <  2:1200245/98‑1 (MQ=255)
              gcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGc                                                                                  <  2:397939/100‑1 (MQ=255)
               cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCt                                                                                 >  2:819814/1‑100 (MQ=255)
               cAGAAGTCTTCGAATGTAGCATTACCTACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGTAACATCCGTCAATTAAAAAAGCGGc                                                                                  <  2:2126098/99‑1 (MQ=255)
                  aaGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAAc                                                                              <  2:1607912/100‑1 (MQ=255)
                     tgtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAc                                                                           <  1:1968846/100‑1 (MQ=255)
                     tgtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTGTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCa                                                                            <  1:1713604/99‑1 (MQ=255)
                      gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcg                                                                          <  2:2674960/100‑1 (MQ=255)
                      gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATATTTCGTTTGCCGGAACATCCGACAATTAAAAAAGCAGCTAACCAc                                                                           >  1:2125203/1‑99 (MQ=255)
                       ttCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgc                                                                         >  1:1953898/1‑100 (MQ=255)
                        tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgcc                                                                        <  2:2656159/100‑1 (MQ=255)
                        tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgcc                                                                        >  2:2969482/1‑100 (MQ=255)
                        tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgcc                                                                        <  1:2472046/100‑1 (MQ=255)
                        tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACAAcgcc                                                                        >  1:2004656/1‑100 (MQ=255)
                          gAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgccgc                                                                      <  1:1196/100‑1 (MQ=255)
                          gAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgccgc                                                                      >  2:674332/1‑100 (MQ=255)
                            aGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtt                                                                    >  1:1739286/1‑100 (MQ=255)
                             tgTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttt                                                                   <  2:2570879/99‑1 (MQ=255)
                               tAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAACAAAGCGGCTAACCACGCCGCttttt                                                                 <  2:2424334/100‑1 (MQ=255)
                               tAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttt                                                                 <  2:1335487/100‑1 (MQ=255)
                               tAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttt                                                                 >  2:1345527/1‑100 (MQ=255)
                                aGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtttttt                                                                <  2:226624/100‑1 (MQ=255)
                                 gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttttt                                                               <  2:1213775/100‑1 (MQ=255)
                                  cATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCATGCCGCTTTTTTTa                                                              <  1:2064525/100‑1 (MQ=255)
                                  cATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                              >  1:1064444/1‑100 (MQ=255)
                                  cATTACCGACCTGCCGAACCAGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAAAATCCGTCAATTACACAAGCAGCTAACCACGCAGCttttttt                                                               >  2:2645524/1‑99 (MQ=255)
                                   aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                              <  1:2618376/99‑1 (MQ=255)
                                   aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTAc                                                             <  1:1682204/100‑1 (MQ=255)
                                    ttACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACg                                                            <  2:2587035/100‑1 (MQ=255)
                                    ttACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACg                                                            <  2:35992/100‑1 (MQ=255)
                                     tACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGt                                                           >  1:222429/1‑100 (MQ=255)
                                      aCCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCATCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc                                                          >  1:1054780/1‑100 (MQ=255)
                                         gACCTGCCGAACCCGAAAGCGAAGCAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc                                                       >  1:190494/1‑100 (MQ=255)
                                         gACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc                                                       >  1:14665/1‑100 (MQ=255)
                                         gACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc                                                       <  2:2947088/100‑1 (MQ=255)
                                         gACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc                                                       >  2:1226662/1‑100 (MQ=255)
                                          aCCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCa                                                      <  1:1910281/100‑1 (MQ=255)
                                           ccTGCCGATCCCGAAAGCGAAGAAGAATGAATTTTTCGTTTGCCGGAACATCCTTCAATTAAAAAAGCTGCTGATCACGCCGCTTTTTTTACGTCTg                                                        <  2:938509/97‑1 (MQ=255)
                                           ccTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCaa                                                     >  2:640855/1‑100 (MQ=255)
                                              gCCGAACCCGAAAGCGAAGAAGAATTAATTTTTCGTTTGCCGGAATATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAttt                                                  <  1:861571/100‑1 (MQ=255)
                                              gCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAttt                                                  >  1:777143/1‑100 (MQ=255)
                                              gCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAttt                                                  >  2:1704882/1‑100 (MQ=255)
                                              gCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAttt                                                  >  2:1368469/1‑100 (MQ=255)
                                               ccGAACCCGAAAGCGTAGAAGAAGTAATTTTGCGTTTGCCGGAACAGCCGTCAATTAAAAAAGCGGCTAACCACGCCGTTTTTTTTACGTATGAAAtt                                                   <  2:283401/98‑1 (MQ=255)
                                               ccGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTa                                                 >  2:2830932/1‑100 (MQ=255)
                                                cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc                                                >  1:2739482/1‑100 (MQ=255)
                                                cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc                                                >  1:2931180/1‑100 (MQ=255)
                                                 gAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAcc                                               <  1:819818/100‑1 (MQ=255)
                                                  atcccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCt                                              <  2:2408297/98‑1 (MQ=255)
                                                  aaCCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAcc                                               >  1:975396/1‑99 (MQ=255)
                                                  aaCCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAAAACGCTTTTTTTACGTCTAAAATTTACCt                                              >  1:627531/1‑100 (MQ=255)
                                                   aCCCGAAAGCGAAGAAGAAGTAATTTTTCTTTTGCCTTAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAcc                                               >  2:327967/1‑98 (MQ=255)
                                                   aCCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCtt                                             >  2:896091/1‑100 (MQ=255)
                                                   aCCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCtt                                             <  2:2792651/100‑1 (MQ=255)
                                                    cccGGAAGCGAAGAAGAAGTAAGTTTGCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGTCTAACCATGCCGCTTTTTTTACGTGTGCAAGTTAc                                                <  2:719460/96‑1 (MQ=255)
                                                      cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc                                          <  2:826143/100‑1 (MQ=255)
                                                       gAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGC‑TTTTTTACGTCTGCAATTTACCTTTCCAg                                        <  2:315482/100‑1 (MQ=255)
                                                        aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAg                                        <  2:1847479/100‑1 (MQ=255)
                                                        aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAg                                        <  1:613582/100‑1 (MQ=255)
                                                        aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAg                                        <  2:576681/100‑1 (MQ=255)
                                                              aagaagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                  <  1:916843/100‑1 (MQ=255)
                                                              aagaagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGATGCTATCCACGTCGCTTTTTTTACGTCTGCAATTTACCTGTCCAGtctgct                                  <  2:601669/100‑1 (MQ=255)
                                                                  agaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCt                              >  1:3018782/1‑100 (MQ=255)
                                                                   gaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTc                             <  1:121000/100‑1 (MQ=255)
                                                                   gaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTc                             <  1:302444/100‑1 (MQ=255)
                                                                    aagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTcc                            <  1:1482721/100‑1 (MQ=255)
                                                                     agTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCa                           >  1:774560/1‑100 (MQ=255)
                                                                     agTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCa                           >  1:651002/1‑100 (MQ=255)
                                                                     agTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTACa                           >  1:3035151/1‑100 (MQ=255)
                                                                      gTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCAc                          >  1:159365/1‑100 (MQ=255)
                                                                        aaTTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGt                        >  2:1451294/1‑100 (MQ=255)
                                                                          tttttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTc                      >  1:1967390/1‑100 (MQ=255)
                                                                              tCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagag                  <  1:856821/100‑1 (MQ=255)
                                                                               cGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagag                  <  2:2294365/99‑1 (MQ=255)
                                                                                gTTTGCCGGAACATCCGTCTATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagagag                >  2:2917449/1‑100 (MQ=255)
                                                                                  ttGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGAc              >  1:1512975/1‑100 (MQ=255)
                                                                                   tGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACg             >  1:514385/1‑100 (MQ=255)
                                                                                    gCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGt            >  1:1773871/1‑100 (MQ=255)
                                                                                    gCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGt            <  1:2558102/100‑1 (MQ=255)
                                                                                     ccGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCATTCTTCTTGTTCCACGTTCAGAGAGAAGtt           >  2:278866/1‑100 (MQ=255)
                                                                                       ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCTACGTTCAGAGAGACGTTCg         <  2:1330874/100‑1 (MQ=255)
                                                                                       ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg         >  1:1827264/1‑100 (MQ=255)
                                                                                        gAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGc        <  2:386769/100‑1 (MQ=255)
                                                                                        gAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGc        <  2:1967367/100‑1 (MQ=255)
                                                                                         aaCATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCa       <  2:1423629/100‑1 (MQ=255)
                                                                                         aaCATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTCCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCa       <  2:1623490/100‑1 (MQ=255)
                                                                                          aCATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCAt      >  1:2845651/1‑100 (MQ=255)
                                                                                            aTCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATAc    <  1:1200904/100‑1 (MQ=255)
                                                                                             tCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATAct   <  1:2235658/100‑1 (MQ=255)
                                                                                              ccGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATActg  >  2:807643/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTG  >  NC_000913/129263‑129456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑