Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,298,278 T→A 18.2% intergenic (‑157/‑320) adhE ← / → ychE fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,298,2780TA18.2% 247.3 / 45.8 104intergenic (‑157/‑320)adhE/ychEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/UPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (45/40);  new base A (6/13);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

Only 100 of 110 total aligned reads displayed.

TATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACA  >  NC_000913/1298179‑1298376
                                                                                                   |                                                                                                  
tataGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGt                                                                                                    >  2:1539671/1‑100 (MQ=255)
     tCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGttatta                                                                                               <  2:1890690/100‑1 (MQ=255)
     tCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGttatta                                                                                               <  1:2614432/100‑1 (MQ=255)
     tCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGttatta                                                                                               <  1:1481680/100‑1 (MQ=255)
         tGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTAtctc                                                                                           >  1:1547627/1‑100 (MQ=255)
          gCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                          <  1:1121856/100‑1 (MQ=255)
          gCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                          >  2:511650/1‑100 (MQ=255)
          gCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                          >  1:2118816/1‑100 (MQ=255)
          gCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                          <  2:2336833/100‑1 (MQ=255)
           cGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTa                                                                                         <  2:2751991/100‑1 (MQ=255)
             gTGATGATTTGCTTAACCTATGAAAATCAAAATCTTACTCGCTCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAAt                                                                                       <  2:2985734/100‑1 (MQ=255)
             gTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAAt                                                                                       >  1:2726509/1‑100 (MQ=255)
              tgatgaTTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAAt                                                                                       >  2:1876350/1‑99 (MQ=255)
                atgatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCg                                                                                    <  1:164876/100‑1 (MQ=255)
                 tgatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgttt                                                                                   <  2:1954679/100‑4 (MQ=38)
                  gatTTGGTTAATCTATGAAAATCAAAAGCTTACTCTCTCTCACACTCACTGTAATTTACTAAAAGAGTTTAAACATTATAGATAATAACTCTAATgttt                                                                                   <  2:1945590/99‑4 (MQ=255)
                   atTTGCTTAACCTATGAAAATCAAATGCTTACTCGCTCTCACACTTACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACGCTAATgtttaa                                                                                 <  1:201474/100‑6 (MQ=255)
                   atTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaa                                                                                 <  1:2040577/100‑6 (MQ=38)
                   atTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaa                                                                                 <  1:608213/100‑6 (MQ=38)
                    tttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACATTCATTGTGATTTATTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaa                                                                                 <  2:127869/99‑6 (MQ=37)
                     ttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGATATAATAACTCTAATgtttaaac                                                                               <  2:1755514/100‑8 (MQ=37)
                     ttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaaac                                                                               <  1:2405660/100‑8 (MQ=37)
                      ttcTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaaact                                                                              <  2:1838568/98‑9 (MQ=37)
                      tGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACtt                                                                              >  2:22210/1‑100 (MQ=255)
                        cTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTTATTTACTAAAAGAGTTTAAACATTAGAGATAATAACTCTAATgtttaaa                                                                                <  2:2914353/96‑7 (MQ=37)
                          tAACCTATGAAAATCAAAAGCTTACTCGCGCTCGCACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAg                                                                          >  2:2005850/1‑100 (MQ=255)
                          tAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAg                                                                          >  1:2871027/1‑100 (MQ=255)
                            aCCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGt                                                                        <  1:868293/100‑1 (MQ=255)
                               tATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGc                                                                     >  1:1308590/1‑100 (MQ=255)
                               tATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGc                                                                     >  1:2682/1‑100 (MQ=255)
                               tATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGc                                                                     <  1:2167920/100‑1 (MQ=255)
                                aTGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCg                                                                    <  2:1583362/100‑1 (MQ=255)
                                 tGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGt                                                                   >  1:1937445/1‑100 (MQ=255)
                                  gAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTa                                                                  >  1:1811143/1‑100 (MQ=255)
                                    aaaTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAg                                                                >  2:542284/1‑100 (MQ=255)
                                      aTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCa                                                              >  1:664595/1‑100 (MQ=255)
                                      aTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCa                                                              >  1:1867791/1‑100 (MQ=255)
                                        cAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAg                                                            <  1:1871449/100‑1 (MQ=255)
                                          aaaGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGAt                                                          <  1:1256960/100‑1 (MQ=255)
                                                tACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCa                                                    >  1:1508414/1‑100 (MQ=255)
                                                 aCTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCAc                                                   <  1:1452896/100‑1 (MQ=255)
                                                  cTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACt                                                  >  1:1276163/1‑100 (MQ=255)
                                                    cgcgCTCACACTCACTGTGATTTACTAAAAGAGTTTAGACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTc                                                >  2:1621976/1‑100 (MQ=255)
                                                    cgcgCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTc                                                >  2:1288738/1‑100 (MQ=255)
                                                    cgcgCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTc                                                <  1:1626312/100‑1 (MQ=255)
                                                     gcgcTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCt                                               <  2:181753/100‑1 (MQ=255)
                                                      cgcTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTg                                              >  1:31284/1‑100 (MQ=255)
                                                      cgcTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTg                                              <  1:36353/100‑1 (MQ=255)
                                                       gcTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTgg                                             <  1:1322020/100‑1 (MQ=255)
                                                        cTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTggg                                            >  1:1624119/1‑100 (MQ=255)
                                                         tCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGt                                           >  2:2234106/1‑100 (MQ=255)
                                                         tCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGt                                           <  2:2370103/100‑1 (MQ=255)
                                                            cactcactGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACt                                        >  1:864395/1‑100 (MQ=255)
                                                            cactcactGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACt                                        >  2:2400936/1‑100 (MQ=255)
                                                                 actGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTa                                   <  1:1885776/100‑1 (MQ=255)
                                                                  cttttaTTTACTAAAAGAGTTTAAACATTATAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTa                                   <  1:1885766/94‑1 (MQ=255)
                                                                  ctGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGTTGGCGTAAGCATGATTACTCACTTCTGGGTACTGATTAc                                  >  2:2646/1‑100 (MQ=255)
                                                                  ctGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTAc                                  <  1:70614/100‑1 (MQ=255)
                                                                   tgtgATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACg                                 >  1:2415136/1‑100 (MQ=255)
                                                                    gtgATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGt                                >  1:724934/1‑100 (MQ=255)
                                                                     tgATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTg                               <  2:2837549/100‑1 (MQ=255)
                                                                       aTTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTTATGCTTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGAt                             <  1:762627/100‑1 (MQ=255)
                                                                       aTTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGAt                             <  1:2603408/100‑1 (MQ=255)
                                                                        tttACTAAAAGAGTTTAAACATTAGAGTTATTTTCTCTTATGCTTCACTTCCAGGTGGCGTAAGAAAGATTACTCACTTCTGGGTACTGATTACGTGATc                            <  1:874252/100‑1 (MQ=255)
                                                                          tACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCca                          <  1:2516721/100‑1 (MQ=255)
                                                                           aCTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaa                         >  2:815412/1‑100 (MQ=255)
                                                                            cTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAATATTACTCACTTCTGGGTACTGATTACGTGATCcaaa                        <  2:2642013/100‑1 (MQ=255)
                                                                            cTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaa                        <  1:1266933/100‑1 (MQ=255)
                                                                            cTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGAGTACGTGATCcaa                         >  2:2027205/1‑99 (MQ=255)
                                                                             tAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaa                        >  2:3029208/1‑99 (MQ=255)
                                                                              aaaaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatc                      >  1:1247973/1‑100 (MQ=255)
                                                                              aaaaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatc                      >  1:1532841/1‑100 (MQ=255)
                                                                               atagagTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGAACTGATTACGTGATCaaaataa                     >  1:2056186/3‑98 (MQ=255)
                                                                               aaaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTTCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatca                     <  2:1940816/100‑1 (MQ=255)
                                                                               aaaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatca                     >  2:2370961/1‑100 (MQ=255)
                                                                                aaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatcaa                    <  1:1030506/100‑1 (MQ=255)
                                                                                aaGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatcaa                    <  1:1031606/100‑1 (MQ=255)
                                                                                    gTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttt                >  1:2789304/1‑100 (MQ=255)
                                                                                    gTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttt                >  1:2810640/1‑100 (MQ=255)
                                                                                     tttAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAtttt               >  1:122901/1‑100 (MQ=255)
                                                                                      ttAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttttt              <  2:1247961/100‑1 (MQ=255)
                                                                                      ttAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttttt              >  1:2650455/1‑100 (MQ=255)
                                                                                      tgacgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttttt              >  1:71156/6‑100 (MQ=38)
                                                                                       tAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             <  2:2708281/100‑1 (MQ=255)
                                                                                       tAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             <  2:2789272/100‑1 (MQ=255)
                                                                                       tAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             <  1:1705392/100‑1 (MQ=255)
                                                                                       gacgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTg             >  1:2031406/5‑100 (MQ=38)
                                                                                         cgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCa           >  1:3001156/3‑100 (MQ=255)
                                                                                         cgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCa           >  2:2021210/3‑100 (MQ=255)
                                                                                         cgcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCa           >  2:2418866/3‑100 (MQ=255)
                                                                                         aaCATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCa           <  2:122900/100‑1 (MQ=255)
                                                                                          gcATTAGAGATAATAACTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCaa          >  1:482288/2‑100 (MQ=255)
                                                                                           cATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTACTGGGTACTGATTACGTGATCTAAATCAAATTTTTGCaaa         >  1:506950/1‑100 (MQ=255)
                                                                                            aTTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAg        <  1:2726010/100‑1 (MQ=255)
                                                                                              tAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCt      >  1:1772244/1‑100 (MQ=255)
                                                                                              tAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCt      >  1:2586737/1‑100 (MQ=255)
                                                                                               agagTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTg     <  2:2794242/100‑1 (MQ=255)
                                                                                                gagTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCt      >  2:2351517/1‑98 (MQ=255)
                                                                                                 atttattaTCTCTAATGCGTCACGTACAGGTGGCGTAATCAAAATTACTCACTTCTGAATACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTg     <  2:1755915/96‑1 (MQ=255)
                                                                                                  gTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGaca  <  2:59519/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACA  >  NC_000913/1298179‑1298376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: