Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 162,973 G→A 100% C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913162,9730GA98.5% 411.5 / ‑4.0 127C290Y (TGT→TAT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (60/64);  total (60/67)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 127 total aligned reads displayed.

TCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGG  >  NC_000913/162877‑163069
                                                                                                |                                                                                                
tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGc                                                                                               <  2:2219748/100‑1 (MQ=255)
tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                               <  2:1256318/100‑1 (MQ=255)
tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                               <  2:1704661/100‑1 (MQ=255)
tcctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGc                                                                                               >  2:2126733/1‑100 (MQ=255)
 cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcc                                                                                              <  2:2859466/100‑1 (MQ=255)
  ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             <  2:1069800/100‑1 (MQ=255)
  ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             <  2:2149819/100‑1 (MQ=255)
  ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             >  1:1553599/1‑100 (MQ=255)
   tccCGGCACCGCAAGTGATGCGCACAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAATGTATTCGTCTGGTGGTGGATTATGCCCa                                                                                            <  2:929239/100‑1 (MQ=255)
   tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCa                                                                                            <  1:2743767/100‑1 (MQ=255)
    cccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAg                                                                                           <  1:2649128/100‑1 (MQ=255)
         cACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                      >  2:532537/1‑100 (MQ=255)
            cGCAAGGTATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTATTGGATTATGCCCAGGAGCttg                                                                                    <  2:2892355/99‑1 (MQ=255)
            cGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtgt                                                                                   >  2:1430013/1‑100 (MQ=255)
                aGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcg                                                                               >  2:917767/1‑100 (MQ=255)
                aGGGATGCGCAAAGTGGCGCTGGCGACCAATATTGCTGAAACCAGTGTAACCATTGAAGGTATTAGACTGGTGGTGGACTATGCCCAGAAGCGagtg                                                                                  >  2:7601/1‑97 (MQ=255)
                    aTGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtt                                                                           <  2:2660792/100‑1 (MQ=255)
                     tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTTGTGGTTTTTGCCCAGGAGCGTGTGGCGCGttt                                                                          <  2:254713/100‑1 (MQ=255)
                     tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGttt                                                                          <  2:1097729/100‑1 (MQ=255)
                     tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAAGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGttt                                                                          >  1:1043635/1‑100 (MQ=255)
                     tGCGCAAAGTGGTGCTGACGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGttt                                                                          <  1:2771247/100‑1 (MQ=255)
                      gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  1:1138250/100‑1 (MQ=255)
                       cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  1:1607392/99‑1 (MQ=255)
                       cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTg                                                                        >  1:160836/1‑100 (MQ=255)
                       cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTAGTCTGGTGGTGGATTATGACCAGGAGCGTGTGGCGCGTTTTg                                                                        >  1:2511667/1‑100 (MQ=255)
                         cAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                       <  1:1229546/99‑1 (MQ=255)
                         cAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGAt                                                                      >  2:1172216/1‑100 (MQ=255)
                          aaaGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATc                                                                     <  2:1713103/100‑1 (MQ=255)
                          aaaGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATc                                                                     <  1:2400320/100‑1 (MQ=255)
                            aGTGGTGCTGGCGACCAATATTGCTGATTCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                                   <  1:146594/100‑1 (MQ=255)
                            aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                                   <  1:144260/100‑1 (MQ=255)
                             gtggtgCTGGTGAACAATATTGCTGAATCCAGTTTATCTATTGAAGGTATTCGTCTGGGGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATc                                                                     <  1:172430/97‑1 (MQ=255)
                             gtggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgc                                                                  >  1:2774012/1‑100 (MQ=255)
                              tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                                 >  1:1454264/1‑100 (MQ=255)
                                 tgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCAc                                                              >  2:1477966/1‑100 (MQ=255)
                                  gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACg                                                             >  1:2276110/1‑100 (MQ=255)
                                  gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACg                                                             >  1:674689/1‑100 (MQ=255)
                                  gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAACGTGTGGCGCGTTTTGATCCGCGCACg                                                             >  1:1374438/1‑100 (MQ=255)
                                      gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                         <  2:2830770/100‑1 (MQ=255)
                                       cGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCt                                                        <  2:572940/100‑1 (MQ=255)
                                          ccAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAc                                                     >  2:1390308/1‑100 (MQ=255)
                                             atatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                                  <  1:2964421/100‑1 (MQ=255)
                                             atatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                                  <  2:2024313/100‑1 (MQ=255)
                                             atatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                                  >  2:376644/1‑100 (MQ=255)
                                              tatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAACGTATAACGCGATTTAATCCGCGCACGGGGCTTACGCGa                                                 >  1:180213/1‑100 (MQ=255)
                                               atTGCTGAAACCAGTTTAACCATTGACGGTATTCGTCTGGTTGTGGATTATGCCCAGGAGCTTATAGAGCGTTTTGATCCGCGCACGGGGCTTACGCAAc                                                >  1:1118632/1‑100 (MQ=255)
                                                ttGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGTTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACt                                               <  1:2629604/100‑1 (MQ=255)
                                                ttGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACt                                               <  2:838265/100‑1 (MQ=255)
                                                ttGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACt                                               <  2:647736/100‑1 (MQ=255)
                                                   cTGAAACCAGTTTAACCATTGAAGGTATTGGTCTGGTGGTGGGTTATGCCCAGGAGCGTGTGGCGCGTTTTTATCCTCTCACGGGGCTTACGTGAc                                                <  2:1451035/96‑1 (MQ=255)
                                                   cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                            >  2:44523/1‑100 (MQ=255)
                                                    tGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCTCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAtt                                           <  2:632356/100‑1 (MQ=255)
                                                    tGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAtt                                           <  2:1033496/100‑1 (MQ=255)
                                                     gAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTa                                          >  2:1805476/1‑100 (MQ=255)
                                                     gAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATGATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAtt                                           <  2:2763738/99‑1 (MQ=255)
                                                      aaaCCAGTTTAATCATTGAAGGTATTCGTCTGGTGGTGGATTATGCTCAGGAGCGTGTGGCGCGTTTTGATCCTCGCACGGGGCTTACGTGACTGATTAc                                         <  2:1111460/100‑1 (MQ=255)
                                                       aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                        >  1:2358535/1‑100 (MQ=255)
                                                        aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                                       <  2:1552198/100‑1 (MQ=255)
                                                        aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGGGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                                       <  1:2208330/100‑1 (MQ=255)
                                                         ccAGTTTAACCATTGAAGGTATTCGTCTGATGGTGGCTTATGCCCAGAAGCGTGTGGCGCGTTTTAATCGGCGCACGGGGCTTACGCGACTGATTATTCa                                      >  2:880349/1‑100 (MQ=255)
                                                           aGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAc                                    <  1:1206386/100‑1 (MQ=255)
                                                           aGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAc                                    <  2:1441223/100‑1 (MQ=255)
                                                            gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                   >  1:375530/1‑100 (MQ=255)
                                                            gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                   <  1:2770848/100‑1 (MQ=255)
                                                              ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                                 <  2:2880860/100‑1 (MQ=255)
                                                              ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                                 <  1:2952782/100‑1 (MQ=255)
                                                               tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                                >  1:2928750/1‑100 (MQ=255)
                                                               tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                                >  2:2878676/1‑100 (MQ=255)
                                                                 aCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTa                              <  2:1415388/100‑1 (MQ=255)
                                                                  ccATTGCAGGTCTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGTCGCGTTTTGATCCGCGCACGGCGCTTACGCGACTGATTACTCAACGCGTTAg                             >  2:1684417/1‑100 (MQ=255)
                                                                  ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCAAGGAGGGTGTGGCGCGTTTTGATCCGCGCTCGGGGCTTACGGGGCTGGTTACTCAACGCGTTa                              >  2:758509/1‑99 (MQ=255)
                                                                   cATTGAAGGTATTCGTCTGGTGGTGGATTATGTCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAgn                            <  1:1395881/100‑2 (MQ=255)
                                                                   cATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGc                            >  1:349532/1‑100 (MQ=255)
                                                                    aTTGAATGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGc                            <  1:2577590/99‑1 (MQ=255)
                                                                      tGAAGGTATTCGTCTGGTGATGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCa                          >  2:2042466/1‑99 (MQ=255)
                                                                       gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        >  1:1689125/1‑100 (MQ=255)
                                                                       gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        <  2:1241184/100‑1 (MQ=255)
                                                                         aGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGGTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa                      <  2:1141989/100‑1 (MQ=255)
                                                                          ggTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCAt                     >  2:2854944/1‑100 (MQ=255)
                                                                             aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa                  <  2:1242941/100‑1 (MQ=255)
                                                                             aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa                  >  1:1961031/1‑100 (MQ=255)
                                                                               tCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATg                <  1:478611/100‑1 (MQ=255)
                                                                                cGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGa               >  1:859211/1‑100 (MQ=255)
                                                                                 gTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc              >  2:2074182/1‑100 (MQ=255)
                                                                                  tCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACg             >  1:2955783/1‑100 (MQ=255)
                                                                                     ggtggtggATTATGTCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAg          >  1:1457035/1‑100 (MQ=255)
                                                                                     ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCa           >  1:607817/1‑99 (MQ=255)
                                                                                     ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAg          >  1:607779/1‑100 (MQ=255)
                                                                                     ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAg          >  1:2006327/1‑100 (MQ=255)
                                                                                     ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAAACAGGCATCCATGACGCa           >  1:1757781/1‑99 (MQ=255)
                                                                                       tggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCg        >  2:2892481/1‑100 (MQ=255)
                                                                                       tggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCg        >  2:1599378/1‑100 (MQ=255)
                                                                                       tggtggATTATGACCAGGAGCGTGTGGCGCGTTTTGATCAGCGCACGGGGCTTACGCGACTGATGACTCAACAGGTTAGGCAGGCATCCATGAAGCAGCg        >  2:304242/1‑100 (MQ=255)
                                                                                        ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt       <  1:2012731/100‑1 (MQ=255)
                                                                                        ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt       <  1:1272903/100‑1 (MQ=255)
                                                                                         gtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTg      <  1:917773/100‑1 (MQ=255)
                                                                                           ggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGc     >  2:822178/1‑99 (MQ=255)
                                                                                            gATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCg   <  1:869287/100‑1 (MQ=255)
                                                                                             aTTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCgg  >  2:1982670/1‑100 (MQ=255)
                                                                                             aTTATGCCCAGGAGCGTGTGGCGCATTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCgg  <  2:1315344/100‑1 (MQ=255)
                                                                                                |                                                                                                
TCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGG  >  NC_000913/162877‑163069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: