Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,833 G→C 15.2% intergenic (‑90/+21) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8330GC15.2% 327.4 / 46.3 127intergenic (‑90/+21)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (54/51);  new base C (8/11);  total (62/65)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

Only 100 of 130 total aligned reads displayed.

TTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTC  >  NC_000913/1441735‑1441932
                                                                                                  |                                                                                                   
nttcttcTTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATAAAAGGTCATTGTGGATTATCTGAATCAGCTCCCCTGGa                                                                                                    >  2:701227/2‑100 (MQ=255)
 ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGaa                                                                                                   <  2:216890/100‑1 (MQ=255)
  tcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAt                                                                                                  >  1:1073554/1‑100 (MQ=255)
  tcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAt                                                                                                  >  2:1733280/1‑100 (MQ=255)
  tcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAt                                                                                                  >  1:1657918/1‑100 (MQ=255)
      cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                              >  2:1255305/1‑100 (MQ=255)
      cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                              >  2:201768/1‑100 (MQ=255)
       aTTGTGGTTCTCAATTACAGTTTCTGACTCAGTACTATTTTAAGAATAGAGGATTAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                             <  2:444891/100‑1 (MQ=255)
       aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                             <  1:2839635/100‑1 (MQ=255)
       aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                              >  1:635923/1‑99 (MQ=255)
        ttGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAggg                                                                                            >  2:320055/1‑100 (MQ=255)
           tgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAg                                                                                         <  1:1423421/100‑1 (MQ=255)
           tgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAg                                                                                         <  2:1050192/100‑1 (MQ=255)
           tgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                        <  2:2718016/100‑1 (MQ=255)
            ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                        <  2:407209/100‑1 (MQ=255)
             gTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCg                                                                                       <  2:840614/100‑1 (MQ=255)
              ttCTCAATTACAGTTTCTGACTCATGACTATTTTAATAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                      <  1:1186145/100‑1 (MQ=255)
              ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                      >  1:1613676/1‑100 (MQ=255)
              ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctg                                                                                      <  1:2820185/100‑3 (MQ=255)
              ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctg                                                                                      <  2:2249162/100‑3 (MQ=255)
              ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctg                                                                                      <  2:808448/100‑3 (MQ=255)
               tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGc                                                                                     <  1:777346/100‑1 (MQ=255)
                  cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAg                                                                                  <  1:2534489/100‑1 (MQ=255)
                   aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGa                                                                                 >  2:1426626/1‑100 (MQ=255)
                     ttACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                               <  1:788896/100‑1 (MQ=255)
                        cAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                            >  1:1169338/1‑100 (MQ=255)
                        cAGTTTCGGACTCAGGACTATTTTAAGAATAGAGGATGAAATGTCATTGGGGATTATCTGAATCAGCTCCCCTGTAATGCAGGGGAGCGGCAAGATTaaa                                                                            <  2:1481469/100‑1 (MQ=255)
                         aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGTGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                           <  1:913922/100‑1 (MQ=255)
                          gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                          <  1:2687024/100‑1 (MQ=255)
                          gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                          <  1:2253228/100‑1 (MQ=255)
                             tCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgt                                                                       <  1:3011316/100‑1 (MQ=255)
                              cTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgtt                                                                      >  2:718816/1‑100 (MQ=255)
                               tGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttc                                                                     >  1:133884/1‑100 (MQ=255)
                               tGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttc                                                                     >  1:1407069/1‑100 (MQ=255)
                                gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                    <  1:2459187/100‑1 (MQ=255)
                                gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                    <  1:1672631/100‑1 (MQ=255)
                                 aCTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCACACCTGGAATGAAGGGGAGCAGCAAGATTAAACCAgatcg                                                                    >  1:974410/1‑99 (MQ=255)
                                  cTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgtt                                                                  >  2:2861391/1‑100 (MQ=255)
                                  cTCAGGACTATTTTAAGAATAGAAGATGAAAGGTCATTGGGGATTATCTGAATTAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgt                                                                   >  2:747033/1‑99 (MQ=255)
                                   tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTTAATTAGCTCCCCTGTAATGCAGGGGAGCGGCAAGATTAAACCAgttcgt                                                                   <  2:1260492/98‑1 (MQ=255)
                                   tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgttc                                                                 >  2:1672073/1‑100 (MQ=255)
                                    cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGttcgttcg                                                                >  2:2472953/1‑100 (MQ=255)
                                      ggACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCggg                                                              <  2:1571426/100‑1 (MQ=255)
                                      ggACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCggg                                                              >  2:2655338/1‑100 (MQ=255)
                                      ggACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCggg                                                              >  2:2488368/1‑100 (MQ=255)
                                       gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                             <  1:911363/100‑1 (MQ=255)
                                         cTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTACCCTGGAATGCAGGGAAGCGGCAAGATTAAACCAGTTCGTTCGGGCa                                                            >  1:92129/1‑99 (MQ=255)
                                          tATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGACTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAgg                                                          <  2:1657898/100‑1 (MQ=255)
                                           aTTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGt                                                         >  2:1510265/1‑100 (MQ=255)
                                              ttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTc                                                      <  2:67531/100‑1 (MQ=255)
                                              ttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTc                                                      >  2:1711790/1‑100 (MQ=255)
                                                aaGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGc                                                    <  2:2972627/100‑1 (MQ=255)
                                                 aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                   >  2:2633856/1‑100 (MQ=255)
                                                  gAATAGAGGATGAAAGGTCATTGGGGTTTATCTGAATCAGCTGCCCTGCAATGCAGGGGAGTGGCAAGATTAATCCAGTTCGTTCGGGCAGGTTTCGcc                                                   <  2:1041613/99‑1 (MQ=255)
                                                  gAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCt                                                  <  2:1028842/100‑1 (MQ=255)
                                                   aaTAGAGGATGAAAGGTCATGGGGGATTATCTGAATCTGCTCCCCTGGAATGCAAGGGAGCTGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                   <  2:2116204/98‑1 (MQ=255)
                                                     tAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCtttt                                               >  1:2266049/1‑100 (MQ=255)
                                                      agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCttttt                                              <  1:2624743/100‑1 (MQ=255)
                                                       gagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTc                                             >  2:955480/1‑100 (MQ=255)
                                                         ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCa                                           <  1:201770/100‑1 (MQ=255)
                                                          gATGAAATGTCATTGGGGATTATCTCAATCATCTCCCCTGGAATCCAGGTGAGCGGCAAGATTAAACCAGTTCGTTCGGGCATGTTTCGCCTTTTTcc                                            <  2:1040124/98‑1 (MQ=255)
                                                            tGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGAt                                        >  1:1157032/1‑100 (MQ=255)
                                                            tGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGAt                                        >  1:2163065/1‑100 (MQ=255)
                                                              aaaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTg                                      <  2:448686/100‑1 (MQ=255)
                                                              aaaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTg                                      >  1:713162/1‑100 (MQ=255)
                                                              aaaGGTCATTGGGGATTAGCTGAATCAGCTCCCCTGGAAGGCAGGGGAGCGGCAAGATTAAACCAGTACGTTCGGGCAGGTTTCGCCTTTTTCCAGAt                                        >  2:1530317/1‑98 (MQ=255)
                                                                aGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCt                                    <  1:2767558/100‑1 (MQ=255)
                                                                aGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCt                                    <  1:2655370/100‑1 (MQ=255)
                                                                 ggTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCTGCAAGATTAAACCAGTTCGTTTGGGCAGGTTTCGCGTTTTTCCAGATTGCt                                    <  2:1421547/99‑1 (MQ=255)
                                                                 ggTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCtt                                   <  2:826710/100‑1 (MQ=255)
                                                                 ggTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCtt                                   >  1:2933313/1‑100 (MQ=255)
                                                                    cATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                 >  1:1347273/1‑99 (MQ=255)
                                                                    cATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                 >  2:946518/1‑99 (MQ=255)
                                                                     aTTTGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAg                                <  1:663553/99‑1 (MQ=255)
                                                                     aTTGGGGATTATCTGAATCAGCTCCCCTGTAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGt                               <  1:1296400/100‑1 (MQ=255)
                                                                          ggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGc                          <  2:952263/100‑1 (MQ=255)
                                                                            aTTATGTGAATCAGCTTCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCTTTTTTTTAGATTGCTTAAGTTTTGCAg                        <  2:1272680/100‑1 (MQ=255)
                                                                             ttATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGc                       <  1:1859273/100‑1 (MQ=255)
                                                                               aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                     >  2:1623610/1‑100 (MQ=255)
                                                                                 cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                   >  1:1896209/1‑100 (MQ=255)
                                                                                 cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGACAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                   >  1:487144/1‑100 (MQ=255)
                                                                                   cttgccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCATTTTTAAAAATTGCTTAAGATTTGCAGCGTAGTc                 >  1:2473480/7‑100 (MQ=25)
                                                                                    ttgccgCTCCCCTGCATTCCAGGGGAGCTGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCt                >  1:407649/6‑100 (MQ=37)
                                                                                       ccgCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGCTTGCTTAAGTTTTGCAGCGTAGTCTga              >  1:2852361/3‑99 (MQ=255)
                                                                                       ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCTAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             >  2:1788391/3‑100 (MQ=255)
                                                                                       cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             <  2:1407056/100‑1 (MQ=255)
                                                                                       cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             <  1:2861423/100‑1 (MQ=255)
                                                                                        cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            >  1:549627/2‑100 (MQ=255)
                                                                                        cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            >  1:635198/2‑100 (MQ=255)
                                                                                        aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            >  1:1837793/1‑100 (MQ=255)
                                                                                          cTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa          <  1:2343539/100‑1 (MQ=255)
                                                                                          cTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa          <  1:756005/100‑1 (MQ=255)
                                                                                           tCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAt         <  2:1162625/100‑1 (MQ=255)
                                                                                            ccccTGGAATGCAGGGGAGCGGCAAGATTAAACAAGTACGTTAGGGCAGATTTAGCCTTTTTCCAGATTGATTAAGTTTTGCAACGTAGTCTGAGaaa          >  2:2425441/1‑98 (MQ=255)
                                                                                               cTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTg     >  2:1125231/1‑100 (MQ=255)
                                                                                               cTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTg     >  1:1482172/1‑100 (MQ=255)
                                                                                                tGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg    <  1:1510282/100‑1 (MQ=255)
                                                                                                tGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg    <  1:2432499/100‑1 (MQ=255)
                                                                                                  gAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            <  2:2743238/90‑1 (MQ=255)
                                                                                                  gAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTTAGAAATACTgggc  <  1:2060459/100‑3 (MQ=255)
                                                                                                  |                                                                                                   
TTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTC  >  NC_000913/1441735‑1441932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: