Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,448,947 A→C 100% T477P (ACG→CCG)  feaB → phenylacetaldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,448,9470AC100.0% 283.6 / NA 86T477P (ACG→CCG) feaBphenylacetaldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (41/45);  total (41/45)

ATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAAC  >  NC_000913/1448853‑1449043
                                                                                              |                                                                                                
atatAGCGATCGCTTACAGGCAGGGACGGTGTTGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGc                                                                                             <  2:1797225/100‑1 (MQ=255)
  ataGCGATCGATTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCg                                                                                           <  2:935956/100‑1 (MQ=255)
   taGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGt                                                                                          >  2:307727/1‑100 (MQ=255)
    aGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTg                                                                                         <  1:598445/100‑1 (MQ=255)
    aGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTg                                                                                         <  2:105427/100‑1 (MQ=255)
       gATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAAGACCGGGCCGTGAtt                                                                                      >  1:2943584/1‑100 (MQ=255)
          cGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTg                                                                                   <  2:2748470/100‑1 (MQ=255)
          cGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTg                                                                                   >  2:2713974/1‑100 (MQ=255)
          cGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTg                                                                                   <  2:706752/100‑1 (MQ=255)
           gCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTgg                                                                                  >  2:2304780/1‑100 (MQ=255)
            cTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTgg                                                                                  <  2:2943552/99‑1 (MQ=255)
            cTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGc                                                                                 >  1:1239246/1‑100 (MQ=255)
             ttACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTg                                                                                   <  1:1308005/97‑1 (MQ=255)
             ttACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGcc                                                                                <  1:1912806/100‑1 (MQ=255)
             ttACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGcc                                                                                <  2:1636361/100‑1 (MQ=255)
              tACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGccc                                                                               <  2:1131348/100‑1 (MQ=255)
                cttgcagaGACGGTGTGGTTAAACAGCAATACCTTAATTGACGCTAACTTAACGGTTAGGGCGATGAAGCAGTCAGGACCCGGCCGTGAattt                                                                                    >  2:1777597/4‑93 (MQ=255)
                    caggGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAAATTACAGTTTGGTGGAATGAAGCAGTCAGGACAGGGCAGTGATGTTGGc                                                                                 >  2:2252829/1‑92 (MQ=255)
                     aggGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGActg                                                                         <  2:2081584/99‑1 (MQ=255)
                       ggACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGActggct                                                                      >  1:1107243/1‑100 (MQ=255)
                        gACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGActggctg                                                                     <  1:491370/100‑1 (MQ=255)
                           ggTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGAc                                                                  >  1:1771851/1‑100 (MQ=255)
                            gtgtGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACg                                                                 >  2:1128962/1‑100 (MQ=255)
                             tgtgGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACgg                                                                >  1:1178420/1‑100 (MQ=255)
                             tgtgGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCAGTGATTTTGGACCCGACTGGCTGGACgg                                                                >  1:664983/1‑100 (MQ=255)
                              gtgGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGt                                                               <  2:1107234/100‑1 (MQ=255)
                              gtgGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGt                                                               >  2:2582627/1‑100 (MQ=255)
                              gtgGGTAAACAGCCATACCTTAATTGACGCTAACGTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGt                                                               <  2:2103236/100‑1 (MQ=255)
                               tgGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGtt                                                              <  2:1052553/100‑1 (MQ=255)
                               tgGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGtt                                                              <  1:2201608/100‑1 (MQ=255)
                                gggTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTg                                                             >  1:2403203/1‑100 (MQ=255)
                                gggTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTg                                                             >  2:1523966/1‑100 (MQ=255)
                                gggTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTg                                                             >  2:1204310/1‑100 (MQ=255)
                                gggTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTg                                                             >  2:467642/1‑100 (MQ=255)
                                 ggTAAACAGCCATACCTTAATTGACGTTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTgg                                                            <  1:2808440/100‑1 (MQ=255)
                                 ggTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTgg                                                            <  1:3000904/100‑1 (MQ=255)
                                 ggTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTgg                                                            <  2:2827472/100‑1 (MQ=255)
                                 ggTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTgg                                                            <  1:2703211/100‑1 (MQ=255)
                                   tAAACAGCCATACCTTAATTGACGCTAACTTCCCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGgtg                                                          >  1:1807990/1‑100 (MQ=255)
                                   tAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGgtg                                                          >  1:900849/1‑100 (MQ=255)
                                   tAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGAACCGGGCCATGATTTTGGCTCCGACTGGCTGGACGGTTGgtg                                                          >  1:219057/1‑100 (MQ=255)
                                   tAAACAGCCATACCTTAATTAACGATAACATACCGATTGGTGAGATTAATCAGACAGGACATTGCCGTGATTATGGCACAGACTCGCTGCACGGt                                                               >  2:927104/1‑95 (MQ=255)
                                    aaaCAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGgtgt                                                         <  2:1178409/100‑1 (MQ=255)
                                     aaCAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGtgtg                                                        >  1:2775201/1‑100 (MQ=255)
                                       cAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGaa                                                      <  2:2940727/100‑1 (MQ=255)
                                       cAGCCATACCTTAATTGACGCTAACTTACCGTTTGGGGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGaa                                                      <  2:2435215/100‑1 (MQ=255)
                                         gCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTAGCCCCGACTGGCTGGACGGTTGGTGTGAAAc                                                    >  1:250275/1‑100 (MQ=255)
                                           cATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTa                                                  >  1:2749477/1‑100 (MQ=255)
                                               ccTTAATTGACGCTAACTTACCGTTTGGTGGGATTAAGCAGTCAGGACCGGGCCATGAATTTGGTCCCGACTGGCTGGACGGTTGGTGTAAAACTAAGTc                                              >  1:1718486/1‑100 (MQ=255)
                                               ccTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTc                                              <  2:1930130/100‑1 (MQ=255)
                                               ccTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTc                                              <  1:307730/100‑1 (MQ=255)
                                               ccTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGACCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTc                                              >  2:1693773/1‑100 (MQ=255)
                                                 ttAATTGACGCTAGCTTACCGTTTGGTGGTATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCgg                                            <  1:1761887/100‑1 (MQ=255)
                                                 ttAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCgg                                            <  1:2521830/100‑1 (MQ=255)
                                                   aaTTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGgtg                                          <  2:2200220/100‑1 (MQ=255)
                                                       gACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCTTGATTTTGGCCCCGACTGGCTGGACGAATGATATAAAACTAAGTCGgtgtg                                        >  1:332655/1‑98 (MQ=255)
                                                         cGCTAACTTACCGTTTGGTAGGATGAAGCAGTCAGGACCGGGACGTGATTTTGGCCCCGACGGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTa                                    >  2:522783/1‑100 (MQ=255)
                                                           cTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACg                                  >  1:2423414/1‑100 (MQ=255)
                                                            tAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGTACGGTTGGTGTGAAACTAAGTCGgtgtgtg                                      <  2:2803571/95‑1 (MQ=255)
                                                            tAACTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACgg                                 <  2:2468285/100‑1 (MQ=255)
                                                              aCTTACCGATTGGTGGGATGAAGCAGTCAGGACGGGGCAGCGATTTTGGCCACGACTGACTGGACGGTTGGTGTGACAGTAAGTCGGTGTGTGTCCg                                  >  2:2960368/1‑97 (MQ=255)
                                                               cTTACCGTTTGGTGGGATGAAGCAGTCAGGACTGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTAt                              <  1:213450/100‑1 (MQ=255)
                                                               cTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGATACTAAGTCGGTGTGTGTACGGTa                               <  2:728384/99‑1 (MQ=255)
                                                               cTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTAt                              >  1:1600616/1‑100 (MQ=255)
                                                               cTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTAt                              >  1:1338122/1‑100 (MQ=255)
                                                               cTTACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTAGACGGTTGATGTGAAACTAAGTCGGTGTGTGTACGGTAt                              >  1:245704/1‑100 (MQ=255)
                                                                 tACCGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTa                            <  2:543663/100‑1 (MQ=255)
                                                                   ccGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAAt                          <  1:2155687/100‑1 (MQ=255)
                                                                   ccGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGACTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAAt                          >  1:768355/1‑100 (MQ=255)
                                                                    cGTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATc                         <  2:1440406/100‑1 (MQ=255)
                                                                     gTTTGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCt                        <  1:2999865/100‑1 (MQ=255)
                                                                      tttGGTGGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTg                       <  1:1012723/100‑1 (MQ=255)
                                                                        tggtggGATGAAGCTGTCAGGACCGGGCCGTGATTTGGGTCCCGACTGGCTGTACGGTTGGCGTGAAACTAAGTCGGTGTTGGTATGGTATTAATCTGGt                     <  2:1018855/100‑1 (MQ=255)
                                                                         ggtggGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGtt                    <  2:2591262/100‑1 (MQ=255)
                                                                               aTGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCa              <  1:2213391/100‑1 (MQ=255)
                                                                                 gAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATa            <  2:1454148/100‑1 (MQ=255)
                                                                                 gAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATa            >  2:101708/1‑100 (MQ=255)
                                                                                  aaGCAGTCAGGACCGGGCCGTGATTTTTGCTCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATaa           <  1:1452299/100‑1 (MQ=255)
                                                                                  aaGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATaa           >  2:1290172/1‑100 (MQ=255)
                                                                                   aGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAg          >  1:1674118/1‑100 (MQ=255)
                                                                                   aGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAg          >  2:331013/1‑100 (MQ=255)
                                                                                   aGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGATCATAAg          <  2:249374/100‑1 (MQ=255)
                                                                                    gCAGCCAGGACCAGGCCGTGACTTTGTCCCCGACTGGCTGGACGGTTGATGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCACTCATAAg          >  2:1089114/1‑99 (MQ=255)
                                                                                      aGTCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGATGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTaa       <  1:417/100‑1 (MQ=255)
                                                                                       ntCAGGACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTaaa      >  2:2337493/2‑100 (MQ=255)
                                                                                           ggACCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAAc  >  2:2838756/1‑100 (MQ=255)
                                                                                              |                                                                                                
ATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAAC  >  NC_000913/1448853‑1449043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: