Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,525,135 (A)6→5 coding (9/765 nt) yfeN → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,1300A.92.7% 266.9 / 15.6 68coding (4/765 nt)yfeNputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base . (30/33);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCG  >  NC_000913/2525031‑2525223
                                                                                                   |                                                                                             
ggCGATATTAATTCCTTTGCTTTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGa                                                                                               >  1:1192536/1‑100 (MQ=255)
ggCGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGa                                                                                               >  1:110901/1‑100 (MQ=255)
 gCGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaa                                                                                              >  1:111344/1‑100 (MQ=255)
  cGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaa                                                                                             <  2:1383593/100‑1 (MQ=255)
   gATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaa                                                                                            >  2:1071223/1‑100 (MQ=255)
   gATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaa                                                                                             >  2:1071311/1‑99 (MQ=255)
   gATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaa                                                                                             >  2:542216/1‑99 (MQ=255)
     tatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaac                                                                                          <  2:211116/100‑2 (MQ=255)
      atTAATTCCTTTGCATTAACAATTAGTCGTTTTTAGCAAACCACCTTATTAAGCAAGCTACTAAGAACGATAGGCATAGGGAATTATGCTATGAAAAAAc                                                                                         <  1:200252/100‑1 (MQ=255)
         aaTTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCt                                                                                     >  1:1899207/1‑100 (MQ=255)
            tCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCtttt                                                                                  <  2:1817848/100‑1 (MQ=255)
             ccTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTa                                                                                 <  2:831719/100‑1 (MQ=255)
             ccTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTa                                                                                 <  1:607727/100‑1 (MQ=255)
              cTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTaa                                                                                >  2:460580/1‑100 (MQ=255)
              cTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTaa                                                                                >  1:1617842/1‑100 (MQ=255)
                  gCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAActct                                                                            >  1:603895/1‑100 (MQ=255)
                  gCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAActct                                                                            >  2:931198/1‑100 (MQ=255)
                   cATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTg                                                                           <  1:733301/100‑1 (MQ=255)
                     ttAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGac                                                                         <  2:874420/100‑1 (MQ=255)
                     ttAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGac                                                                         <  2:96320/100‑1 (MQ=255)
                      tAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGaca                                                                        >  1:151957/1‑100 (MQ=255)
                        aCAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACt                                                                      >  2:1564517/1‑100 (MQ=255)
                        aCAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACt                                                                      >  1:1007619/1‑100 (MQ=255)
                           atatGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTc                                                                   >  1:1882464/1‑100 (MQ=255)
                             atGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCct                                                                 >  1:1170532/1‑100 (MQ=255)
                             atGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCct                                                                 <  2:110901/100‑1 (MQ=255)
                               gtgggttttAGGAACCCTCTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCtt                                                                                    <  2:1261396/75‑1 (MQ=255)
                               gTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctct                                                               >  2:477577/1‑100 (MQ=255)
                                tCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCta                                                              <  1:502273/100‑1 (MQ=255)
                                 cGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtat                                                             <  2:373690/100‑1 (MQ=255)
                                 cGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtat                                                             >  2:1164027/1‑100 (MQ=255)
                                  gTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtata                                                            <  1:1783748/100‑1 (MQ=255)
                                       tAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGc                                                       <  1:1747939/100‑1 (MQ=255)
                                        aGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCg                                                      <  1:477575/100‑1 (MQ=255)
                                          cAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGAt                                                    <  2:1587824/100‑1 (MQ=255)
                                           aaaCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATa                                                   >  1:1236754/1‑100 (MQ=255)
                                           aaaCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATa                                                   >  1:1900688/1‑100 (MQ=255)
                                             aCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATAcc                                                 <  1:1380426/100‑1 (MQ=255)
                                             aCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATAcc                                                 <  2:1547356/100‑1 (MQ=255)
                                             aCCACTTTATTAAGCAAGCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATAcc                                                 <  1:361702/100‑1 (MQ=255)
                                                aCTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgt                                              >  1:1544908/1‑100 (MQ=255)
                                                 cTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgtt                                             >  1:467562/1‑100 (MQ=255)
                                                 cTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgtt                                             >  1:464922/1‑100 (MQ=255)
                                                  tttATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgttg                                            <  2:1784799/100‑1 (MQ=255)
                                                    tattaAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTa                                          <  2:1192538/100‑1 (MQ=255)
                                                     attaAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTa                                          <  1:484864/100‑1 (MQ=255)
                                                      ttaAGAAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTAtc                                        <  1:1703965/100‑1 (MQ=255)
                                                       taAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTAtct                                       >  1:847159/1‑100 (MQ=255)
                                                       taAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTAtct                                       >  1:982019/1‑100 (MQ=255)
                                                        aaGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTAtctc                                      >  1:1135417/1‑100 (MQ=255)
                                                         aGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCa                                     <  2:1526653/100‑1 (MQ=255)
                                                          gCAATCTACTAATAACTATCGGCATAGGGAATTATGCTCTGAAAAACATCTTTTAACTCTGACGCTTTCCTCTATTTTGGCGATACCTGTTGTATCTCAt                                    <  1:538938/100‑1 (MQ=255)
                                                          gCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCAt                                    <  2:1170534/100‑1 (MQ=255)
                                                            aaTCTACTAATAACGATCGGCATATGGAAAAAAAAAAAAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCa                                     <  1:143659/97‑1 (MQ=255)
                                                              tCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAg                                <  1:931197/100‑1 (MQ=255)
                                                              tCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTCCCTCTATATTAGCGATACCTGTTGTATCTCATGCAg                                >  2:1849692/1‑100 (MQ=255)
                                                               ctactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGa                               <  1:879977/100‑1 (MQ=255)
                                                               ctactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGa                               >  2:1708001/1‑100 (MQ=255)
                                                                tactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGaa                              >  2:124629/1‑100 (MQ=255)
                                                                tactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGaa                              >  1:404110/1‑100 (MQ=255)
                                                                tactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGaa                              <  2:271384/100‑1 (MQ=255)
                                                                tactaATAACGATCGGCATAGGGAATAATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGa                               >  2:1188590/1‑100 (MQ=255)
                                                                 actaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAAt                             <  1:1419419/100‑1 (MQ=255)
                                                                  ctaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAAtt                            <  2:935264/100‑1 (MQ=255)
                                                                       aaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAg                       >  2:189258/1‑100 (MQ=255)
                                                                         cGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGc                     <  1:460578/100‑1 (MQ=255)
                                                                          gATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCg                    >  1:1350181/1‑100 (MQ=255)
                                                                                cATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTg              >  2:1272844/1‑100 (MQ=255)
                                                                                  tAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa            <  2:1899220/100‑1 (MQ=255)
                                                                                  tAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa            <  2:1148825/100‑1 (MQ=255)
                                                                                  tAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa            >  1:1213353/1‑100 (MQ=255)
                                                                                   aGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAg           <  2:586209/100‑1 (MQ=255)
                                                                                     ggAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGat         >  2:245401/1‑100 (MQ=255)
                                                                                        aTTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATc      <  1:1164025/100‑1 (MQ=255)
                                                                                          tATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCgg    >  1:368583/1‑100 (MQ=255)
                                                                                            tGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCg  <  2:1236758/100‑1 (MQ=255)
                                                                                                   |                                                                                             
GGCGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCG  >  NC_000913/2525031‑2525223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: