| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,500,814 | Δ1 bp | intergenic (‑23/‑446) | yjgX ← / → yjgZ | pseudogene fragment/uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,500,811 | 0 | T | . | 97.0% | 293.1 / 2.2 | 67 | intergenic (‑20/‑449) | yjgX/yjgZ | pseudogene fragment/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base . (27/38); total (28/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GTCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGGCCT > NC_000913/4500712‑4500909 | gtCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAgtgt > 1:1472442/1‑100 (MQ=255) tCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtt < 1:164711/100‑1 (MQ=255) ccGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGttt > 1:372118/1‑100 (MQ=255) cGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttc > 1:918402/1‑99 (MQ=255) cGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttc > 1:1842286/1‑99 (MQ=255) cGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttc > 1:502285/1‑99 (MQ=255) cGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttc < 1:1632374/100‑2 (MQ=255) gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttct < 2:410399/100‑3 (MQ=255) cAGAACATCACCAGGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAATCACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttctg < 1:155336/99‑4 (MQ=255) cAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttctgt > 1:142910/1‑96 (MQ=255) aGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTa < 1:148208/100‑1 (MQ=255) aGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTa < 2:907443/100‑1 (MQ=255) aaatTCACCACGAATACCAGAATCTCGGCACTGACCAGAAAATGTTAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAgg < 1:9015/100‑1 (MQ=255) aaTCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGtt < 2:455637/100‑1 (MQ=255) caccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTg < 2:757029/100‑1 (MQ=255) accacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGa > 1:767637/1‑100 (MQ=255) accacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGa > 1:1274808/1‑100 (MQ=255) ccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTg < 1:629040/98‑1 (MQ=255) ccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGaa < 1:20772/100‑1 (MQ=255) ccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGaa < 1:20670/100‑1 (MQ=255) acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAAcc < 2:372120/100‑1 (MQ=255) acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAAcc < 2:1615990/100‑1 (MQ=255) acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAAcc < 1:1287465/100‑1 (MQ=255) aCCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCt > 2:788841/1‑100 (MQ=255) aCCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCt < 1:982495/100‑1 (MQ=255) aCCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTCCt > 2:1545008/1‑100 (MQ=255) cAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAg > 1:272832/1‑100 (MQ=255) tCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCgggg < 2:278924/100‑1 (MQ=255) tCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCgggg < 2:1736560/100‑1 (MQ=255) cgcgGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGt > 2:583515/1‑100 (MQ=255) gcgGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGtt < 2:263072/100‑1 (MQ=255) ggCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTtgt > 2:946531/1‑100 (MQ=255) cACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGa > 2:1104377/1‑100 (MQ=255) aCTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAg < 2:1130691/100‑1 (MQ=255) cTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAgg < 1:1506252/100‑1 (MQ=255) tGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGc > 2:1478891/1‑100 (MQ=255) gACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCt > 1:509243/1‑100 (MQ=255) ccAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGa > 2:1379042/1‑100 (MQ=255) aGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATg < 2:1492239/100‑1 (MQ=255) gAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATgg < 2:415364/100‑1 (MQ=255) gAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATgg > 2:1473565/1‑100 (MQ=255) tAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGcc > 2:963128/1‑100 (MQ=255) aaCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCgg > 2:1923094/1‑100 (MQ=255) aaCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCgg < 2:1335634/100‑1 (MQ=255) cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGc > 1:258753/1‑100 (MQ=255) cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGc < 1:1788115/100‑1 (MQ=255) cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGc < 2:1039917/100‑1 (MQ=255) cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGc < 1:1392702/100‑1 (MQ=255) aGGAAACACGGGGCTGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGc < 2:159060/100‑1 (MQ=255) gAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGAG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGctct > 2:1368134/1‑100 (MQ=255) gAAACACGGGCCGGCTCACTCTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGCTCTAGCGGCGTTGTGAGGCTGATGGCAATCGc > 1:1780116/1‑92 (MQ=255) acacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTcc < 2:502287/100‑1 (MQ=255) cacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCt > 2:783789/1‑100 (MQ=255) acGGGGCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTg < 2:142911/100‑1 (MQ=255) ggggCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGc > 1:1604169/1‑99 (MQ=255) gggCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCa > 2:1496781/1‑99 (MQ=255) ggCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATg < 2:1472450/100‑1 (MQ=255) ggCGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCCTAACCGGTTCTAGCGGGGTCGTGAGGCTGATGGCAATCGCCGAGCTCTTCCTGCATg < 2:29150/100‑1 (MQ=255) cGGTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGtt > 2:87348/1‑100 (MQ=255) cGGTTCACTTTGCTTTCTCCTGAAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGCTGGCAATCGCCGGGCTCTTCCTGCATGtt > 1:1478429/1‑100 (MQ=255) gTTCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTac < 2:1611586/100‑1 (MQ=255) tCACTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTacac < 2:1369512/100‑1 (MQ=255) aCTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTg > 1:1817558/1‑100 (MQ=255) cTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTg > 2:372434/1‑100 (MQ=255) cTTTGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg < 2:545051/100‑1 (MQ=255) tGCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTa < 2:279646/99‑1 (MQ=255) agCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTAc < 2:825046/99‑1 (MQ=255) gCTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACt > 2:681420/1‑100 (MQ=255) cTTTCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTg < 1:1251182/100‑1 (MQ=255) tttCTCCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGc < 2:431398/100‑1 (MQ=255) ctcCTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAag < 2:1260743/100‑1 (MQ=255) ccTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAagag > 1:1789972/1‑100 (MQ=255) ccTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCCCTTCCTGCATGTTACACTGGTACTGCAagag > 1:582438/1‑100 (MQ=255) cTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAgg < 2:1842298/100‑1 (MQ=255) cTGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAgg < 2:1413937/100‑1 (MQ=255) tGTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggg < 2:767638/100‑1 (MQ=255) gTAGTG‑TTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTCGTACTGCAAGAgggg > 2:1909562/1‑100 (MQ=255) gtTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGGCCt > 1:332195/1‑100 (MQ=255) | GTCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGGCCT > NC_000913/4500712‑4500909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |