| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,073,307 | Δ1 bp | coding (705/1149 nt) | rutA ← | pyrimidine oxygenase, FMN‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,073,305 | 0 | A | . | 93.7% | 254.1 / 11.0 | 63 | coding (707/1149 nt) | rutA | pyrimidine oxygenase, FMN‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base . (29/30); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
TCGTCGGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAG > NC_000913/1073206‑1073400 | tcgtcgGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGa < 1:1312182/100‑1 (MQ=255)tcgtcgGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGa < 1:787489/100‑1 (MQ=255) ggTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGaagccg > 1:101196/1‑96 (MQ=255) ggTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGaagccg > 1:1859497/1‑96 (MQ=255) tttCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTg < 1:1893532/100‑1 (MQ=255) tCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTggg < 2:1813188/100‑1 (MQ=255) aTCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGgtg > 2:845749/1‑100 (MQ=255) cGGCAATCACCATAAGCAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTa < 2:1914486/100‑1 (MQ=255) ggCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTa > 2:52851/1‑100 (MQ=255) ggCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTa < 1:797855/100‑1 (MQ=255) aaTCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTa < 1:427257/100‑1 (MQ=255) aaTCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTa > 2:528589/1‑100 (MQ=255) aCCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGcc > 2:1044483/1‑100 (MQ=255) aCCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGcc < 2:489704/100‑1 (MQ=255) cATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCtt > 2:158420/1‑100 (MQ=255) aacaacACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGcc < 1:1871351/100‑1 (MQ=255) aacaacACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGcc > 1:227210/1‑100 (MQ=255) aacaacACAGAAGAGCCAACGTCGCGTCCGGTTTGCTGTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGc < 1:506398/99‑1 (MQ=255) acaacaCATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGACCTGTTTCATCCGCGCAGCAGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCg > 1:1063699/1‑100 (MQ=255) caacaCATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGa < 2:1205995/100‑1 (MQ=255) caacaCATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGa > 2:803270/1‑100 (MQ=255) cacaTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAAc < 2:1325341/100‑1 (MQ=255) acaTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACa < 2:193405/100‑1 (MQ=255) aTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGa > 1:1396697/1‑100 (MQ=255) tAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGaa > 1:1628502/1‑100 (MQ=255) aaGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAg < 1:1705629/100‑1 (MQ=255) agagCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGt < 2:1768852/100‑1 (MQ=255) gagCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGt < 1:1887024/100‑1 (MQ=255) gagCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGtt < 2:1472605/100‑1 (MQ=255) agCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTg > 2:353323/1‑100 (MQ=255) gCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa < 2:1085872/100‑1 (MQ=255) gCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa > 1:1681863/1‑100 (MQ=255) gCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa > 1:174287/1‑100 (MQ=255) ccAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGaa < 2:1385008/100‑1 (MQ=255) cAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGaaa > 2:660401/1‑100 (MQ=255) cGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCg > 2:1295109/1‑100 (MQ=255) tCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGc < 2:452664/100‑1 (MQ=255) cgcgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCa < 1:710431/100‑1 (MQ=255) gcgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCa < 1:1744113/99‑1 (MQ=255) gTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATAc > 1:79833/1‑100 (MQ=255) ccGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCg > 2:599194/1‑100 (MQ=255) ccGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCg < 2:1562790/100‑1 (MQ=255) tttGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AATCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCg < 1:1248532/100‑1 (MQ=255) ttGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCgg < 2:608519/100‑1 (MQ=255) gCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgag > 2:107571/1‑100 (MQ=255) ctctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgag > 1:1652325/1‑100 (MQ=255) ctctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgaga < 2:352460/100‑1 (MQ=255) tGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACg < 1:525143/100‑1 (MQ=255) gCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGaagg > 1:1013705/1‑97 (MQ=255) gCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGc > 2:1462249/1‑100 (MQ=255) ccTGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATg > 2:821828/1‑100 (MQ=255) tGTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGcc > 2:775603/1‑100 (MQ=255) gTTTCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCa > 1:1488100/1‑100 (MQ=255) ttCATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGc > 2:1001804/1‑100 (MQ=255) cATCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGt > 2:1359682/1‑100 (MQ=255) aTCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGTCATACCGGGCGGAGAACGCCATGCCAGCGTc > 2:473794/1‑100 (MQ=255) aTCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTc > 2:1145629/1‑100 (MQ=255) aTCCGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTACCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTc > 1:1081529/1‑100 (MQ=255) ccGCGCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGc > 2:1357518/1‑100 (MQ=255) cgcgCAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCt > 2:1939326/1‑100 (MQ=255) cgcAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCttt < 1:219410/100‑1 (MQ=255) gcAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTg < 1:643787/100‑1 (MQ=255) gcAGCGGTCGGGGCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTg < 1:1393723/100‑1 (MQ=255) gTCGGGGCG‑AAGCCGTGAGTGACTTTAAGCCTTTGCCGAAACGGAAGTAGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGccc < 1:448169/97‑1 (MQ=255) ggggCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGgcgcn < 2:1534519/100‑2 (MQ=255) gggCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAg < 2:1628512/100‑1 (MQ=255) gggCG‑AAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAg < 1:607682/100‑1 (MQ=255) | TCGTCGGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAG > NC_000913/1073206‑1073400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |