| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,170,338 | (A)6→5 | coding (37/963 nt) | ldtC ← | L,D‑transpeptidase linking Lpp to murein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,170,333 | 0 | A | . | 97.1% | 309.9 / 1.4 | 70 | coding (42/963 nt) | ldtC | L,D‑transpeptidase linking Lpp to murein |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base . (32/36); total (32/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGA > NC_000913/1170235‑1170432 | tGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaa < 1:1211957/100‑1 (MQ=255)tGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaa > 1:1444065/1‑100 (MQ=255)tGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaa > 2:903742/1‑100 (MQ=255) gAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaa > 2:13091/1‑100 (MQ=255) ttGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacgt < 1:1836386/100‑4 (MQ=255) ttGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTGGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacgt < 1:1335884/100‑4 (MQ=255) gTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTa > 2:734480/1‑100 (MQ=255) ttttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAg < 1:833105/100‑1 (MQ=255) ttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGcc < 1:459627/100‑1 (MQ=255) cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAg > 2:426559/1‑100 (MQ=255) cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAg > 2:710010/1‑100 (MQ=255) cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAg > 2:804588/1‑100 (MQ=255) ccAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCg > 2:936950/1‑100 (MQ=255) ccAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCg > 2:936805/1‑100 (MQ=255) cAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCga > 2:1638027/1‑100 (MQ=255) cAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCga < 2:547169/100‑1 (MQ=255) ccAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACATTAGCCAGCGAGaa < 2:626671/100‑1 (MQ=255) cAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGaaa > 1:1050914/1‑100 (MQ=255) aGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGaaaa > 2:1328488/1‑100 (MQ=255) gacgacTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGaaaaa > 2:560672/1‑100 (MQ=255) acacctggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAAc > 2:1690459/9‑100 (MQ=37) cgacTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAAcg < 1:605209/100‑1 (MQ=255) ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAAcgcg > 2:452460/2‑100 (MQ=255) tGCCCGCGGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGtt < 1:327755/100‑1 (MQ=255) ncccGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGttt > 2:1126425/2‑100 (MQ=255) gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGttt < 2:1461904/100‑1 (MQ=255) gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGttt > 2:1555190/1‑100 (MQ=255) cccGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGtttt < 2:1035576/100‑1 (MQ=255) cggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAtcatca < 1:656479/100‑1 (MQ=255) ggCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGAAAGAGAGAAAAACGCGTTTTGATCATCACCaaaaa < 1:710009/100‑1 (MQ=255) ccAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATc < 1:426557/100‑1 (MQ=255) aGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa < 2:381984/100‑1 (MQ=255) ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat > 1:1051157/1‑100 (MQ=255) gtgtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCata < 1:262801/100‑1 (MQ=255) gtgtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCata < 2:1790519/100‑1 (MQ=255) tGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCAtaataa > 2:39367/1‑100 (MQ=255) ggCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaa > 2:702212/1‑100 (MQ=255) ggCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAAAAAAAAAAAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaa < 1:605917/100‑1 (MQ=255) ggCTTTTCCCGATAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaa > 1:1025549/1‑100 (MQ=255) gCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaat < 1:930814/100‑1 (MQ=255) gCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaat < 1:538778/100‑1 (MQ=255) cTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaata > 2:173076/1‑100 (MQ=255) cTTTTGCCGGTAGCGCCAGCAACACGGCAGCGGCGAACGTAAAAAACGTTAAAAAGAAAAAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaa < 1:1715818/99‑1 (MQ=255) tttGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAAtata > 2:1451246/1‑100 (MQ=255) ttGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGAAAAAAAAAAAAAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATaa < 1:903741/100‑1 (MQ=255) ccGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGt > 1:782453/1‑100 (MQ=255) cGGTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGtt > 2:199475/1‑100 (MQ=255) gTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTAt < 2:682975/100‑1 (MQ=255) gTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTAt < 1:737169/100‑1 (MQ=255) gTAGCGCCAGCGCCACGGCAGCGGCGAACGT‑AAAAACGTTAGAAAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTAt < 1:1459824/100‑1 (MQ=255) agcgccagcgccACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTAtt < 1:265758/100‑1 (MQ=255) agcgccagcgccACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg < 1:813026/100‑1 (MQ=255) agcgccagcgccACGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg < 1:297680/100‑1 (MQ=255) cgccagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGta < 1:1869152/100‑1 (MQ=255) cgccagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATAATCAACAAAAATCCATAATAAATATAAGGTTATTGta < 1:1638017/100‑1 (MQ=255) gccagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTATCCAGCGAGAAAAACGCGTTATGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaa < 1:961773/100‑1 (MQ=255) gccagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaa > 2:798701/1‑100 (MQ=255) gccagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaa > 2:1632102/1‑100 (MQ=255) gccagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCAAGCGAGAAAAACGCGTTATGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaa < 1:961574/100‑1 (MQ=255) cgatctcaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaata < 1:475719/94‑1 (MQ=255) cagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaata < 2:631444/100‑1 (MQ=255) cagcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTATTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaata > 1:836702/1‑100 (MQ=255) gcgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATaaa < 1:148134/100‑1 (MQ=255) cgccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc < 1:1926447/100‑1 (MQ=255) gccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACa > 2:1758924/1‑100 (MQ=255) gccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACa > 2:446048/1‑100 (MQ=255) gccaCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAAAAAAAACAAAAATCCATAATAAATATAAGGTTATTGTAATAAACa < 1:615731/100‑1 (MQ=255) caCGGCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaataagatcg > 2:475721/1‑95 (MQ=255) ggCAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATaa > 1:1072981/1‑100 (MQ=255) cAGCGGCGAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGc > 1:1047100/1‑100 (MQ=255) gcggcgAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCt > 1:1704067/1‑100 (MQ=255) gcgAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGaa > 1:1460708/1‑100 (MQ=255) cgAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAt < 2:7760/100‑1 (MQ=255) cgAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAt < 1:452458/100‑1 (MQ=255) cgAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAt < 2:1008699/100‑1 (MQ=255) gAACGT‑AAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAtt < 2:790812/100‑1 (MQ=255) aaaaaaCGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTatgga < 2:1416618/100‑1 (MQ=255) | TGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGA > NC_000913/1170235‑1170432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |