Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,469,656 (T)7→8 coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,6491.T84.5% 310.5 / 52.7 103coding (519/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base . (10/6);  new base T (39/48);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

Only 100 of 116 total aligned reads displayed.

TGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469552‑2469743
                                                                                                  |                                                                                                 
tGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtt                                                                                                 <  2:1014947/100‑1 (MQ=255)
tGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtt                                                                                                 >  2:1660405/1‑100 (MQ=255)
   ttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttt                                                                                             >  2:756199/1‑100 (MQ=255)
   ttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttt                                                                                             <  1:1524995/100‑1 (MQ=255)
   ttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttt                                                                                             >  2:946638/1‑100 (MQ=255)
   ttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCCTTTGCTAttttt                                                                                             >  2:1778475/1‑100 (MQ=255)
     gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                          <  2:975524/100‑1 (MQ=255)
     gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                          <  2:673921/100‑1 (MQ=255)
      catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttt                                                                                         >  2:431253/1‑99 (MQ=255)
       atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttc                                                                                        <  2:695778/100‑3 (MQ=255)
          atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTg                                                                                    <  1:756198/100‑1 (MQ=255)
            tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTgn                                                                                   <  1:1872924/100‑2 (MQ=255)
            tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                   <  1:1932745/100‑1 (MQ=255)
            tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                   <  2:789495/100‑1 (MQ=255)
             cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                  >  2:932501/1‑100 (MQ=255)
               aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                                <  1:1550165/100‑1 (MQ=255)
                 cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaata                                                                              >  2:529817/1‑100 (MQ=255)
                 cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaata                                                                              >  2:1125792/1‑100 (MQ=255)
                 cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaata                                                                              >  2:1041739/1‑100 (MQ=255)
                  aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCTTTGCtaataa                                                                             >  2:1414262/1‑100 (MQ=255)
                   agagCAAATAGTTATAATTTTCCTTCCATTTTTGTTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            >  1:39185/1‑100 (MQ=255)
                   agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTTCtaataa                                                                             >  2:450644/1‑99 (MQ=255)
                   agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            >  1:999733/1‑100 (MQ=255)
                   agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            >  1:21071/1‑100 (MQ=255)
                    gagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAataata                                                                           <  2:1648694/100‑1 (MQ=255)
                      gCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTCTGCTATTTTTTTTCGTTGATAATAatat                                                                          >  1:1172444/1‑99 (MQ=255)
                         aaTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTAc                                                                      <  1:185031/100‑1 (MQ=255)
                             gTTATAATTTTCCTTCCATTTTTTGTGTATTGATTAATATGCAAAAACAATAAATCTGTGCTTTTGCTATTTTTTTTCGTTTCTAATAATATCTACTTCt                                                                  <  1:568751/100‑1 (MQ=255)
                             gTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCt                                                                  <  2:138118/100‑1 (MQ=255)
                               tataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTaaa                                                               >  2:760708/1‑100 (MQ=255)
                                ataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaa                                                               <  1:989875/100‑1 (MQ=255)
                                 taATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaaa                                                              <  2:30812/100‑1 (MQ=255)
                                 naaTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaaa                                                              >  2:369783/2‑100 (MQ=255)
                                  aaTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCCACTGCTaaaaa                                                             >  2:1683308/1‑100 (MQ=255)
                                   aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTTATAATATATACTGCTAAAAAt                                                            >  1:1116090/1‑100 (MQ=255)
                                   aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATc                                                           >  2:1300695/1‑100 (MQ=255)
                                    ttttCCTTCCATTTTTGGTGTATTCATTATCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTGTTTCGTTGCTAATAATATCTACTGCTAAAAATc                                                           <  1:1172189/100‑1 (MQ=255)
                                        ccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAtt                                                       <  1:750829/100‑1 (MQ=255)
                                        ccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAtt                                                       <  1:1505900/100‑1 (MQ=255)
                                        ccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                      >  2:641689/1‑100 (MQ=255)
                                         cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     >  2:562688/1‑100 (MQ=255)
                                          ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtat                                                    >  1:530087/1‑100 (MQ=255)
                                           tccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtat                                                    >  2:1568962/1‑100 (MQ=255)
                                           tccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     <  1:932500/99‑1 (MQ=255)
                                            ctttgttttGTGTATTCAGTAACATGCAAAAACAATATTTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                   <  2:1330810/95‑1 (MQ=255)
                                            ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                   <  2:1346085/100‑1 (MQ=255)
                                            ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                   <  2:421862/100‑1 (MQ=255)
                                                ttttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTaa                                               >  1:1253095/1‑100 (MQ=255)
                                                ttttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTACTAATAATATCTACTGCTAAAAATCAATTTATATTaa                                               <  1:1041738/100‑1 (MQ=255)
                                                 tttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTaa                                               <  1:781429/99‑1 (MQ=255)
                                                   ttgtgtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTATCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             <  1:1660395/97‑1 (MQ=255)
                                                    ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                           <  2:957444/100‑1 (MQ=255)
                                                    ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                           <  2:182869/100‑1 (MQ=255)
                                                    ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                           <  1:1249963/100‑1 (MQ=255)
                                                    ggTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGTTAAAAATCCATTTATATTAACCCCa                                          >  1:334482/1‑100 (MQ=255)
                                                       gtATTCATTAACATGCAAAAACAATCAATCTATGCTTTTGCTATTTTTTTTCGTTACTAATAATATCTACTGCTAACAATCAATTTATATTAACCCCACt                                        >  1:1668232/1‑100 (MQ=255)
                                                       gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                        <  1:548440/100‑1 (MQ=255)
                                                          ttCATTAACATGCAAAAACAATGAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAAt                                     >  1:1127213/1‑100 (MQ=255)
                                                          ctCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAAt                                     <  2:1563143/99‑1 (MQ=255)
                                                           tCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   <  2:1431606/100‑1 (MQ=255)
                                                            tccgatcttGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCACTTTATATTAACCCCACTAATAg                                   <  1:1128533/92‑1 (MQ=255)
                                                            cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgt                                  >  1:173084/1‑100 (MQ=255)
                                                              ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATAACTAATGATAAAAATCAATTTATATTAACCCCACTAATAgtg                                 <  1:560677/100‑1 (MQ=255)
                                                              ttAACATGCAAAAACAATAAATCGATGCTGTTGCGATTTTTTTTCGTTTCTAATAATATCTACTGGTAAAAATCAATTTATATTAACCCCACTAATAg                                   >  2:875759/1‑98 (MQ=255)
                                                                aaCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTa                               >  1:1701048/1‑100 (MQ=255)
                                                                   aTGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTc                            >  1:971371/1‑100 (MQ=255)
                                                                   aTGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTc                            >  2:622687/1‑100 (MQ=255)
                                                                   aTGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATAACTAATAATAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           <  1:513561/100‑1 (MQ=255)
                                                                    tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           <  2:21071/100‑1 (MQ=255)
                                                                     gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                          >  2:680205/1‑100 (MQ=255)
                                                                     gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                          >  1:1417597/1‑100 (MQ=255)
                                                                     gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         >  1:1853283/1‑100 (MQ=255)
                                                                      cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         >  2:552309/1‑100 (MQ=255)
                                                                      cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         <  1:862712/100‑1 (MQ=255)
                                                                       aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                        <  2:1205868/100‑1 (MQ=255)
                                                                        aaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGTTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatt                       >  1:303752/1‑100 (MQ=255)
                                                                        aaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatt                       >  1:14961/1‑100 (MQ=255)
                                                                         aaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatta                      <  2:61198/100‑1 (MQ=255)
                                                                         aaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatta                      <  2:991456/100‑1 (MQ=255)
                                                                          aaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattat                     >  2:1215581/1‑100 (MQ=255)
                                                                            naaTAAATCTATGCTTTTGCTATTTTTTTCCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatat                   >  1:505269/2‑100 (MQ=255)
                                                                            cAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatat                   >  1:1107516/1‑100 (MQ=255)
                                                                            cAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatat                   <  2:476466/100‑1 (MQ=255)
                                                                                 aaTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt              >  2:1246014/1‑100 (MQ=255)
                                                                                 aaTCTAAGCTTTTGCTATTTTTTTTCGTTGCTAATAAAAAAAACAAAAAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtctttt              <  1:1261501/100‑1 (MQ=255)
                                                                                   tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  2:182974/100‑1 (MQ=255)
                                                                                   tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  1:1614268/100‑1 (MQ=255)
                                                                                    cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttg            >  1:938874/1‑98 (MQ=255)
                                                                                    cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  1:1215578/100‑1 (MQ=255)
                                                                                     tATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  1:362179/99‑1 (MQ=255)
                                                                                     tATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGa          >  1:162111/1‑100 (MQ=255)
                                                                                      aTGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTATCCCCACTAATAGTGTATTCATATTATATTTTTTTTGAt         <  1:1293901/100‑1 (MQ=255)
                                                                                      aTGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGAt         <  1:1857258/100‑1 (MQ=255)
                                                                                       tGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATa        <  1:1349884/100‑1 (MQ=255)
                                                                                         cTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGac     <  2:1253642/100‑1 (MQ=255)
                                                                                          ttttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGan     <  1:369781/100‑2 (MQ=255)
                                                                                           tttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGaca    <  1:1681938/100‑1 (MQ=255)
                                                                                            ttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTATAAATTAATTTATCTTAACCCCATTAATCGTGAATTCATATTATATTTTATTTTATAGaa     >  1:1442118/1‑97 (MQ=255)
                                                                                            ttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGacac   <  1:1611536/100‑1 (MQ=255)
                                                                                            ttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGacac   <  2:1127214/100‑1 (MQ=255)
                                                                                                  |                                                                                                 
TGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469552‑2469743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: