| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 584,262 | (A)7→8 | coding (582/750 nt) | appY → | global transcriptional activator; DLP12 prophage |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 584,255 | 1 | . | A | 89.2% | 239.9 / 22.1 | 75 | coding (575/750 nt) | appY | global transcriptional activator; DLP12 prophage |
| Reads supporting (aligned to +/- strand): ref base . (3/5); new base A (41/25); total (45/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAATTGATTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑AAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTA > NC_000913/584156‑584347 | cGGAATTGATTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑c > 2:846530/1‑100 (MQ=255)cGGAATTGATTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑c < 1:986738/100‑1 (MQ=255) ggAATTGATTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑a < 2:806518/100‑1 (MQ=255) ttGATTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑aaaaa < 2:1632474/100‑1 (MQ=255) tGATTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑aaaaa‑a > 2:179403/1‑100 (MQ=255) gATTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑aaaaa‑aa > 1:1486692/1‑100 (MQ=255) aTTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGCTTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑aaaaa‑aa > 1:228159/1‑99 (MQ=255) aTTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑CAAAA‑AAc < 2:1025106/100‑1 (MQ=255) ttATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑aaaaa‑aaact < 1:791840/100‑4 (MQ=255) tataCGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGAAACGTCATTTACTGAAATATTGAGAGATACTAGGATTAGGTATA‑C‑aaaaa‑aaac > 1:189133/1‑96 (MQ=255) ataCGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑AAAAA‑AACTCAt > 2:1764141/1‑100 (MQ=255) aCGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATa > 2:501408/1‑100 (MQ=255) aCGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATa < 2:1091852/100‑1 (MQ=255) gAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAAc > 2:369536/1‑100 (MQ=255) aGTGAAAGTTTAATAAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTAAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAAc > 1:230420/1‑100 (MQ=255) aGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACt > 2:1592712/1‑100 (MQ=255) gTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACtt > 2:1257018/1‑100 (MQ=255) gTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACtt > 1:1749369/1‑100 (MQ=255) gTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACtt > 1:1390392/1‑100 (MQ=255) gTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑AAAAA‑AACTCATAACTTc < 2:800103/100‑1 (MQ=255) tGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTc > 1:793869/1‑100 (MQ=255) aaaaTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAAAGTCATTTACTGAAATATTGAGAGATACTAGGATGAAAAATA‑AAAAAAA‑AACTCATAACTTCaa < 1:1592701/100‑1 (MQ=255) aaaGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCa > 2:1191614/1‑99 (MQ=255) aGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAAc < 2:179708/100‑1 (MQ=255) at‑aaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTAt > 1:1789198/1‑100 (MQ=255) at‑aaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTAt > 2:100306/1‑100 (MQ=255) at‑aaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑‑AAAA‑AACTCATAACTTCAAACTCTTaat > 2:1724636/1‑97 (MQ=255) aaaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTc > 1:1769070/1‑100 (MQ=255) aaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCt < 1:880753/100‑1 (MQ=255) aaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTa < 2:1033969/100‑1 (MQ=255) aaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑AAAAATAACTCATAGCTTCAAACTCTTATTc < 1:850676/98‑1 (MQ=255) aaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTAt > 1:1904689/1‑100 (MQ=255) aaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATAAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCaa < 2:1485630/100‑1 (MQ=255) gATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATg > 1:506822/1‑100 (MQ=255) aTTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑AAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTc > 2:1776144/1‑100 (MQ=255) ttAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTc > 2:1595581/1‑100 (MQ=255) ttAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTc > 1:1942630/1‑100 (MQ=255) tAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCg < 1:193264/100‑1 (MQ=255) gggATGAAGGAACGTCATTTACTGGAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAg > 2:986520/1‑100 (MQ=255) gATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAgac > 1:1181635/1‑98 (MQ=255) gATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGcc > 1:617360/1‑100 (MQ=255) gATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGcc < 2:1670184/100‑1 (MQ=255) gATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGcc < 2:1579722/100‑1 (MQ=255) aTGAAGGAACATCATTTACTTAAATATTGAGATATTCTAGGATGAAAAAAA‑AAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGccc < 1:407669/100‑1 (MQ=255) tGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCa < 2:1594810/100‑1 (MQ=255) gAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAg < 2:295198/100‑1 (MQ=255) aaGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGa < 1:519867/100‑1 (MQ=255) gAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAt > 2:63408/1‑100 (MQ=255) aaCGTCATTTACTGAAATATTGAGAGATACTAGGATGAAGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAtg < 1:1791536/100‑1 (MQ=255) acatCATTTACTGAAATATTGAGAGATACTAGGAAAAAAAAAAAA‑AAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGaaa < 1:425863/94‑1 (MQ=255) cGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAtgtg < 1:716675/100‑1 (MQ=255) cGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAtgtg > 2:1824990/1‑100 (MQ=255) cGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑AAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTgg < 1:1055338/100‑1 (MQ=255) gTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTgg > 2:1238693/1‑100 (MQ=255) tCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGc < 2:1256/100‑1 (MQ=255) aTTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCta > 2:1659943/1‑100 (MQ=255) tACTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATaa > 2:1821843/1‑100 (MQ=255) tACTGAAATATTGAGAGATACTAGGATGAAAAATA‑AAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCtata < 1:216027/99‑1 (MQ=255) aCTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAAc < 2:1749380/100‑1 (MQ=255) cTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACa > 2:277733/1‑100 (MQ=255) cTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACa < 2:91001/100‑1 (MQ=255) cTGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCACAGAAATGTGGCTATAACa > 1:1058145/1‑100 (MQ=255) tGAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAg < 2:591199/100‑1 (MQ=255) gAAATATTGAGAGATACTAGGATGAGGTATG‑CGAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGt > 1:564530/1‑100 (MQ=255) gAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGGAATGTGGCTATAACAGt > 2:1899066/1‑100 (MQ=255) gAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGt < 2:55084/100‑1 (MQ=255) gAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGt > 2:1682325/1‑100 (MQ=255) gAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGt > 2:178994/1‑100 (MQ=255) gAAATATTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCGTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGt > 1:1364871/1‑100 (MQ=255) tatTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACtt > 1:38072/1‑100 (MQ=255) atTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttc > 1:751394/1‑100 (MQ=255) atTGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttc < 1:1606097/100‑1 (MQ=255) ttGAGAGATACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttca > 1:257402/1‑100 (MQ=255) gagagaTACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcata > 1:878619/1‑100 (MQ=255) gagagaTACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcata > 1:1541166/1‑100 (MQ=255) tACTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcat > 1:713134/1‑100 (MQ=255) aCTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcata > 1:1467569/1‑100 (MQ=255) aCTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcata < 2:564766/100‑1 (MQ=255) aCTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcata < 2:1876485/100‑1 (MQ=255) cTAGGATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcatat > 2:1747015/1‑100 (MQ=255) ggATGAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATAtgtg < 1:1075271/100‑1 (MQ=255) gAGGTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAtt > 1:781674/1‑100 (MQ=255) ggTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTa > 1:1495276/1‑100 (MQ=255) ggTATG‑CAAAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTa > 2:958330/1‑100 (MQ=255) | CGGAATTGATTTATACGAGTGAAAGTTTAAT‑AAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATG‑C‑AAAAA‑AACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTA > NC_000913/584156‑584347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |