| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 649,964 | +G | coding (527/909 nt) | citE ← | citrate lyase, citryl‑ACP lyase (beta) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 649,960 | 1 | . | G | 98.5% | 244.3 / ‑2.8 | 68 | coding (531/909 nt) | citE | citrate lyase, citryl‑ACP lyase (beta) subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (35/32); total (35/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
TGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑GGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGC > NC_000913/649863‑650052 | tgttAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑gg < 2:706330/100‑1 (MQ=255) tAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑ggggg > 2:381161/1‑99 (MQ=255) tAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑ggggg < 2:1507213/100‑2 (MQ=255) tAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑ggggg < 2:23599/100‑2 (MQ=255) gCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑gggggag < 2:803669/100‑4 (MQ=255) gCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑gggggag < 2:1779409/100‑4 (MQ=255) cGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑gggggag > 2:1025197/1‑96 (MQ=255) gTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑gggggagcg > 2:911309/1‑95 (MQ=255) gTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTCCC‑gggggagcg < 2:356725/100‑6 (MQ=255) ggAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTc < 2:104358/100‑1 (MQ=255) ggAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTc > 1:74253/1‑100 (MQ=255) gAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCt < 2:552860/100‑1 (MQ=255) aaTAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTg > 2:1840330/1‑100 (MQ=255) aaTAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTg < 2:1531016/100‑1 (MQ=255) tAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTa < 2:811224/100‑1 (MQ=255) aGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTAc > 1:616392/1‑100 (MQ=255) gACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTAc > 1:303447/1‑99 (MQ=255) gACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACg > 1:735489/1‑100 (MQ=255) aCGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGc > 1:803879/1‑100 (MQ=255) cGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCa > 1:439923/1‑100 (MQ=255) cGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCa < 2:1482411/100‑1 (MQ=255) cGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCa > 2:1641231/1‑100 (MQ=255) gTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAgg < 1:1294238/100‑1 (MQ=255) tATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGt < 2:1123220/100‑1 (MQ=255) aTCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCCGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGtt > 1:84899/1‑100 (MQ=255) aTCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTGCAGTTCCTTCCGGGGGAGCTTTCTGTACGCAGGt > 1:1622384/1‑99 (MQ=255) tCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTg < 1:1346033/100‑1 (MQ=255) cGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTgc > 2:1413044/1‑100 (MQ=255) cGAACGCCTGAATACCCGCAGAGCGCGCGGCCTACAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTgc > 2:127756/1‑100 (MQ=255) gAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTgcg > 1:1687307/1‑100 (MQ=255) aaCGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTgcgc < 2:164041/100‑1 (MQ=255) aCGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGca < 2:608037/100‑1 (MQ=255) aCGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑GGGGAGCGTTCTGTACGCAGGTTGCGcac < 1:973680/100‑1 (MQ=255) gCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGcaca < 2:258918/100‑1 (MQ=255) gCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGcaca > 2:353727/1‑100 (MQ=255) cTGAATACCCTCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATa < 1:1276281/100‑1 (MQ=255) tGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATan < 1:451721/100‑2 (MQ=255) aCCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGtcttc < 2:42108/100‑1 (MQ=255) cccGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGtcttct > 1:1750847/1‑100 (MQ=255) cccGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTGGCGCACATAGtcttct > 2:1167912/1‑100 (MQ=255) cccGCAGAGCGCGCGGCCAGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGtcttct > 2:56999/1‑100 (MQ=255) ccGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTg < 2:1714040/100‑1 (MQ=255) agagCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCAcc < 2:1401893/100‑1 (MQ=255) gagCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCg > 2:1254208/1‑100 (MQ=255) gagCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCg > 1:1337010/1‑100 (MQ=255) agCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGa < 2:542732/100‑1 (MQ=255) ggCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGAt < 2:244756/100‑1 (MQ=255) gCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATa > 2:998214/1‑100 (MQ=255) tGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCg > 2:1682354/1‑100 (MQ=255) tGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCg > 2:1035796/1‑100 (MQ=255) gCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGa > 1:980628/1‑100 (MQ=255) aaaaTGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATc < 1:1581553/100‑1 (MQ=255) aaaaTGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGAGACCGATc < 1:1022023/100‑1 (MQ=255) aaaTGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCa < 2:2230/100‑1 (MQ=255) aaTGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCaa < 2:74253/100‑1 (MQ=255) aTGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCaaa < 2:545372/100‑1 (MQ=255) gAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGc > 1:375249/1‑100 (MQ=255) aCAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTc > 1:520931/1‑100 (MQ=255) cAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCg < 2:1337018/100‑1 (MQ=255) aGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCgg < 2:735490/100‑1 (MQ=255) gCGTGCGAACAGCAGTTCAGTTCCTGCCGGGGGTGCGTTCTGGACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATCCCGATCAAACGCTCGGa < 1:1872300/100‑1 (MQ=255) cGTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGaa > 1:108991/1‑100 (MQ=255) gTGCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAg < 1:1078557/100‑1 (MQ=255) gCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCg > 1:1149917/1‑100 (MQ=255) gCGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCg > 2:538946/1‑100 (MQ=255) cGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGt > 2:444275/1‑100 (MQ=255) cGAACAGCAGTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGt > 1:1151553/1‑100 (MQ=255) cagcagTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGc < 1:381159/100‑1 (MQ=255) cagcagTTCAGTTCCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGc > 2:305490/1‑100 (MQ=255) agttcagttcCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGAtt < 1:1034056/100‑1 (MQ=255) agttcagttcCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGAtt > 2:1418796/1‑100 (MQ=255) gttcagttcCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGAttt > 2:849448/1‑100 (MQ=255) gttcagttcCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGAttt > 2:883392/1‑100 (MQ=255) tcagttcCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTcc > 2:1735049/1‑100 (MQ=255) tcagttcCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTcc < 1:1083092/100‑1 (MQ=255) tcagttcCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTcc < 1:998213/100‑1 (MQ=255) agttcCTTCCGGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCAc < 2:1813116/100‑1 (MQ=255) ttccttccgGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTg < 1:1380017/100‑1 (MQ=255) tccttccgGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTgc < 2:1622394/100‑1 (MQ=255) tacttccgGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTgc > 2:1316010/3‑100 (MQ=255) | TGTTAGCGTCGGAATAGACGGTATCGAACGCCTGAATACCCGCAGAGCGCGCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCC‑GGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGC > NC_000913/649863‑650052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |