Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,530,076 (A)7→8 coding (155/483 nt) ydcD → putative immunity protein for RhsE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,530,0691.A92.1% 340.3 / 21.3 101coding (148/483 nt)ydcDputative immunity protein for RhsE
Reads supporting (aligned to +/- strand):  ref base . (1/7);  new base A (52/41);  total (53/48)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 119 total aligned reads displayed.

TGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGACGC  >  NC_000913/1529970‑1530162
                                                                                                     |                                                                                              
tGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTg                                                                                                 <  1:492261/100‑1 (MQ=255)
tGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTg                                                                                                 <  2:1495178/100‑1 (MQ=255)
tGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTg                                                                                                 <  1:1300221/100‑1 (MQ=255)
tGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTg                                                                                                 <  1:1780592/100‑1 (MQ=255)
tGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTg                                                                                                 <  2:241950/100‑1 (MQ=255)
  tttATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaa                                                                                              >  2:810193/1‑100 (MQ=255)
    tATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaa                                                                                            >  2:1065007/1‑100 (MQ=255)
    tATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaa                                                                                            >  1:1468206/1‑100 (MQ=255)
       ttgttgTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaag                                                                                         >  1:1787797/1‑99 (MQ=255)
       ttgttgTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaaa                                                                                        >  1:11205/1‑100 (MQ=255)
       ttgttgTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaaa                                                                                        >  1:1860011/1‑100 (MQ=255)
         gttgttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaaaat                                                                                      <  2:1078142/100‑3 (MQ=255)
         gttgttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTATGaaaaaaaat                                                                                      <  1:796764/100‑3 (MQ=255)
           tgttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaaaatat                                                                                    >  1:866195/1‑96 (MQ=255)
           tgttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGaaaaaaaatat                                                                                    <  2:1489275/100‑5 (MQ=255)
            gttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGACATATATCTTTCTGaaaaaaaatat                                                                                    >  1:1359285/1‑95 (MQ=255)
              ttACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAAtattat                                                                                  <  2:1522629/100‑1 (MQ=255)
               tACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATattatt                                                                                 >  2:934332/1‑100 (MQ=255)
                 ccTTGGGTTTGATAACAATTCAAATTCTGACATCTTATGTTATTGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATAttattt                                                                                <  2:1135211/99‑1 (MQ=255)
                 ccTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATAttattta                                                                               <  2:1524179/100‑1 (MQ=255)
                 ccTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATAttattta                                                                               >  2:203670/1‑100 (MQ=255)
                  cTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATAttatttat                                                                              >  2:1750063/1‑100 (MQ=255)
                   ttGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTtata                                                                             >  2:378308/1‑100 (MQ=255)
                   ttGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTtata                                                                             >  1:522674/1‑100 (MQ=255)
                   ttGGGTTTGATAACAATTCAAACTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATAttatttat                                                                              >  2:1892433/1‑99 (MQ=255)
                      ggTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATaaaa                                                                          >  2:1554393/1‑100 (MQ=255)
                          tGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAaataat                                                                      >  2:633913/1‑100 (MQ=255)
                           gATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaatt                                                                     <  2:210643/100‑1 (MQ=255)
                           gATAACAATTCAAATTCTGACATCGTATTTTATGAACATAAAACAACAAAGAGCGTTGAGATATAAAAAAAAAAAAAAAATATTATTTATAAAAtaatta                                                                    <  1:705127/100‑1 (MQ=255)
                            aTAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaatta                                                                    >  2:942896/1‑100 (MQ=255)
                            aTAAAAATTCAAATTCTGACATCGTATTTTATGGACATAAAAAACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaatt                                                                     <  1:1821111/99‑1 (MQ=255)
                             tAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaattaa                                                                   >  1:1138797/1‑100 (MQ=255)
                             tAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAAtaattaa                                                                   >  1:1883150/1‑100 (MQ=255)
                               aCAATTCAAATTCTGACATCGTTTTTTATAATAATAACACACCAAAGAGCGTTGAGATAAAAAAAAAAAAAAAAAAATATTATTTATAAAATAATTAATg                                                                 <  1:1310241/100‑1 (MQ=255)
                               aCAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATg                                                                 <  2:1128674/100‑1 (MQ=255)
                                 aaTTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGAc                                                               <  1:1055270/100‑1 (MQ=255)
                                 aaTTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGAc                                                               <  2:120087/100‑1 (MQ=255)
                                  aTTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGAcc                                                              >  1:1537921/1‑100 (MQ=255)
                                  aTTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATATATATAAAAAAAAATATTATTTATAAAATAATTAATGAcc                                                              <  1:1878171/100‑1 (MQ=255)
                                     cAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCaaa                                                           >  1:1551876/1‑100 (MQ=255)
                                         ttCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCaaaaaa                                                        >  1:1903306/1‑99 (MQ=255)
                                             gACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACAAAAAAATtag                                                    <  1:897060/99‑1 (MQ=255)
                                             aacataatATTTTATGAACATAAAACAACAAAGAGCGTTAAGATAAAAAAAAAAAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATtagt                                                   <  1:1512469/92‑1 (MQ=255)
                                              aCATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATtagta                                                  <  2:1394813/100‑1 (MQ=255)
                                               cATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATtagtag                                                 >  1:210429/1‑100 (MQ=255)
                                                aTCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTaga                                                >  2:659288/1‑100 (MQ=255)
                                                  cGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTagag                                               >  1:817569/1‑99 (MQ=255)
                                                  cGTATTTAAAAGAAAAAAAACACCAAAGAGCGTTGAGATATAAAAAAAAAAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAgg                                              <  1:565165/100‑1 (MQ=255)
                                                     aTTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGaa                                           <  2:645583/100‑1 (MQ=255)
                                                     aTTTTATAGACATAAAACACAAAAGAGGGTTGAGATATATCAATATAAAAAAAAATATTATTTATAAAATAAAAAAAAAAAAAAAAATtag                                                    <  1:575496/91‑1 (MQ=255)
                                                         tATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGt                                       >  1:478926/1‑100 (MQ=255)
                                                          aTGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTc                                      <  1:810192/100‑1 (MQ=255)
                                                           tGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCa                                     >  1:1421121/1‑100 (MQ=255)
                                                           tGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCa                                     >  1:1609464/1‑100 (MQ=255)
                                                            ggACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAt                                    >  1:164467/1‑100 (MQ=255)
                                                            ggACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAt                                    <  2:616352/100‑1 (MQ=255)
                                                             gACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt                                   >  2:1803171/1‑100 (MQ=255)
                                                             gACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt                                   >  1:1420385/1‑100 (MQ=255)
                                                              aCATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAttt                                  >  2:1471928/1‑100 (MQ=255)
                                                               cATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtttt                                 >  1:1110175/1‑100 (MQ=255)
                                                               cATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtttt                                 >  1:511070/1‑100 (MQ=255)
                                                                aTAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTta                                <  2:478928/100‑1 (MQ=255)
                                                                 tAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTtat                               >  2:540273/1‑100 (MQ=255)
                                                                  aaaaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTtata                              >  1:1412994/1‑100 (MQ=255)
                                                                  aaaaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTtata                              >  2:748572/1‑100 (MQ=255)
                                                                  aaaaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTtata                              <  2:634215/100‑1 (MQ=255)
                                                                  aaaaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTtata                              <  1:942895/100‑1 (MQ=255)
                                                                   aaaCACCAAATAGCGTTGATATATATCTGTCCGAAAAAAAATAAGATATACAAAATAAGTAATGACCAAAAAATGATTAGAAGGAAAGGTCATGTTATaa                             >  1:873129/1‑100 (MQ=255)
                                                                   aaaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg                            <  2:1673568/100‑1 (MQ=255)
                                                                    aaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg                            >  2:1546618/1‑100 (MQ=255)
                                                                    aaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg                            >  1:1266054/1‑100 (MQ=255)
                                                                    aaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg                            <  2:1330692/100‑1 (MQ=255)
                                                                    aaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg                            <  2:1207267/100‑1 (MQ=255)
                                                                    aaCACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAGATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg                            <  2:1787808/100‑1 (MQ=255)
                                                                     acacCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGt                           >  1:1075621/1‑100 (MQ=255)
                                                                      cacCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGta                          <  2:1075622/100‑1 (MQ=255)
                                                                        ccAAAGAGCGTTGAGATATATCTTTAAGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGta                          <  1:844770/98‑1 (MQ=255)
                                                                          aaaGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAAt                      >  2:460472/1‑100 (MQ=255)
                                                                          aaaGAGCGTTGAGATATATCTTTCTGAAAAAAAATATTATTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATg                     <  1:1065006/100‑1 (MQ=255)
                                                                            agagCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATgg                    <  2:1203090/100‑1 (MQ=255)
                                                                             gagCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGt                   <  1:885120/100‑1 (MQ=255)
                                                                             gagCGTTGAGATATAGCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGt                   <  2:1912515/100‑1 (MQ=255)
                                                                               gCGTTGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTta                 >  2:1350524/1‑100 (MQ=255)
                                                                                  ttGAGATATATTTTTCTGAAAAAAAATAGTATTTATAAAATAATTAATGACCAAAAAAGTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaata              <  1:451922/100‑1 (MQ=255)
                                                                                  ttGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaata              <  1:273025/100‑1 (MQ=255)
                                                                                  ttGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaat               <  1:1750052/99‑1 (MQ=255)
                                                                                   tGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaataa             >  1:1461611/1‑100 (MQ=255)
                                                                                   tGAGATATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaataa             <  1:234205/100‑1 (MQ=255)
                                                                                     agaTATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAAtt           <  2:5840/100‑1 (MQ=255)
                                                                                      gaTATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATTACCAAAAAATTCGTAGAGGAAATGGTTCTATTATAAGTATAATGGTTAATAATTa          >  1:1242036/1‑100 (MQ=255)
                                                                                      gaTATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTa          <  2:1647932/100‑1 (MQ=255)
                                                                                      gaTATATCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTa          >  1:311721/1‑100 (MQ=255)
                                                                                       atatatatTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTa          <  1:232366/99‑1 (MQ=255)
                                                                                       atatatCTTTCTAAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaataa             <  1:1727102/96‑1 (MQ=255)
                                                                                        tatatCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACa        >  1:1049277/1‑100 (MQ=255)
                                                                                        tatatCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACa        >  1:43041/1‑100 (MQ=255)
                                                                                          tatCTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAgg      <  2:1179370/100‑1 (MQ=255)
                                                                                             cTTTCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGACg   >  1:1393455/1‑100 (MQ=255)
                                                                                              tttCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGACGc  <  2:758856/100‑1 (MQ=255)
                                                                                              tttCTGAAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGACGc  <  2:1883162/100‑1 (MQ=255)
                                                                                                     |                                                                                              
TGTTTATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTCTGAAAAAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGGACGC  >  NC_000913/1529970‑1530162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: