Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,071,699 | 0 | C | A | 22.8% | 177.5 / 48.3 | 79 | G23W (GGG→TGG) | rutD | putative reactive intermediate detoxifying aminoacrylate hydrolase |
Reads supporting (aligned to +/- strand): ref base C (30/31); new base A (9/9); total (39/40) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01 |
GTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATGCGCAATTGTG > NC_000913/1071601‑1071797 | gtCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCa < 2:1831153/100‑1 (MQ=255) gtCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCa > 2:375116/1‑100 (MQ=255) tCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCaa > 2:809143/1‑100 (MQ=255) cGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAg > 2:1734161/1‑100 (MQ=255) gggATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGa > 1:1188373/1‑100 (MQ=255) gggATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGa > 2:347562/1‑100 (MQ=255) gATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGAcc < 1:1681901/100‑1 (MQ=255) gATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGAcc > 2:1028150/1‑100 (MQ=255) attattGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGAccc < 1:1540635/100‑1 (MQ=255) ttattGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCg < 1:268466/100‑1 (MQ=255) attGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGaa < 2:829329/100‑1 (MQ=255) tGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAAt > 2:993552/1‑100 (MQ=255) gCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATc < 1:672120/100‑1 (MQ=255) gCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATc < 1:650298/100‑1 (MQ=255) ccGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATcn < 1:699147/100‑2 (MQ=255) ccGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCCCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGCCCCGAAATCa < 1:1453119/100‑1 (MQ=255) ggTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAac < 2:1365406/100‑1 (MQ=255) ggTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAac > 2:1618405/1‑100 (MQ=255) ggTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAac < 1:555140/100‑1 (MQ=255) ccGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAAcacca > 2:761761/1‑100 (MQ=255) cgcgCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAAcaccac < 2:851251/100‑1 (MQ=255) cTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACg > 1:1072645/1‑100 (MQ=255) ggTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACgcg > 1:1208832/1‑98 (MQ=255) gTCGTAACAGACTACCTGATACGCCTGCTCCAGCGCCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACAACTACggg > 1:458726/1‑99 (MQ=255) gTAACAGACTACCTGATACTCCTGCTCCAGCACCTCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCa < 1:1028149/100‑1 (MQ=255) gTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCa > 1:1116988/1‑100 (MQ=255) gTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCa < 1:1340774/100‑1 (MQ=255) aaCAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATc < 2:1921675/100‑1 (MQ=255) aCAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCa < 1:1018625/100‑1 (MQ=255) aCAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCa > 2:611841/1‑100 (MQ=255) aCAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCa < 2:1001951/100‑1 (MQ=255) aGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGc < 1:1827561/100‑1 (MQ=255) gACTACCTGATCCTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGCCCCGAAATCAACACCACTACCGGCGCATCAGCa < 1:719269/100‑1 (MQ=255) gACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCa > 2:1906356/1‑100 (MQ=255) cTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCAt > 1:745704/1‑99 (MQ=255) aCCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAg < 1:524298/100‑1 (MQ=255) ccTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAgg > 1:1612284/1‑100 (MQ=255) cTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAggg < 2:1738712/100‑1 (MQ=255) tGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAgggg > 2:1880615/1‑100 (MQ=255) gATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAggggg < 1:360231/100‑1 (MQ=255) aTACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGa < 2:1892350/100‑1 (MQ=255) aTACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGa < 1:761760/100‑1 (MQ=255) tACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAg > 2:1607091/1‑100 (MQ=255) aCTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAgg < 2:1335650/100‑1 (MQ=255) aCTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAgg < 2:1572318/100‑1 (MQ=255) aCTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAgg < 2:1294544/100‑1 (MQ=255) aCTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAgg < 1:657871/100‑1 (MQ=255) cTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAgg > 2:1415895/1‑99 (MQ=255) cTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAgg > 2:1555384/1‑99 (MQ=255) cTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGt < 1:1439248/100‑1 (MQ=255) cTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCACAAggggg > 1:1820081/1‑96 (MQ=255) ccTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTga > 2:369891/1‑100 (MQ=255) tGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTgaga < 1:1653399/100‑1 (MQ=255) cAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGaa > 2:480037/1‑100 (MQ=255) aGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGaaa > 2:1914807/1‑100 (MQ=255) gCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGTCGCCTCAGCAGCAGGGGGGGGTGaaagcaacag > 1:225112/1‑94 (MQ=255) gCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAg < 1:69579/100‑1 (MQ=255) cACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGCGAGAGTGAAAGt > 1:1356643/1‑100 (MQ=255) ccGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGttt > 1:1761049/1‑100 (MQ=255) cGCCAGTTGCGGTAACCAGTAACTGCGGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTc < 1:653312/100‑1 (MQ=255) gCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCa < 2:993271/100‑1 (MQ=255) cAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCCTAAGGGGGCGGTGAGAGTGAATGTTTc > 1:1113708/1‑97 (MQ=255) gCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAAGACCACTACGGGCGCATCAGCATAAGGGGGAGGTGCGAGTGAAGGTTTCATCGCgg > 1:1331588/1‑99 (MQ=255) tAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCCGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCa > 2:452015/1‑100 (MQ=255) tAACCAGTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCa < 1:1680528/100‑1 (MQ=255) ccAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCCGCATCAGGGCGAGGCGAGAGTCCAAGTTTCAGCGCGGCCTCAcct > 1:401485/1‑98 (MQ=255) ccAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACtt > 2:314619/1‑100 (MQ=255) cAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGCCGCCTCACCATAAGGGGGAGGGGAAAGTGAAAGTTTCTTCGCGTCCTCCCTTg > 1:1455666/1‑100 (MQ=255) aGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTgg > 2:1698545/1‑100 (MQ=255) gTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGACAGTGAAAGTTTCATCGCGGCCTCACTTGGc < 1:1274706/100‑1 (MQ=255) gTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATAAGCATAAGGCGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGc < 2:878558/100‑1 (MQ=255) gTAACTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGc < 1:347560/100‑1 (MQ=255) aCTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGAAGATCGAGAAAGTTTCATCGCGGCCTCACTTGGCGa > 1:1556363/1‑99 (MQ=255) aCTGCCGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGat > 2:453323/1‑100 (MQ=255) tGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGatat < 2:1761060/100‑1 (MQ=255) gCCGCTACCCCCAAGACCCGAAATCAACACCAGTAGGGGCGCATCAGAATACTGGGGAGGTGACGATGACGGTTTCCTCGCGGCCTCCCTTGGCGgtagg > 1:1452525/1‑98 (MQ=255) ccGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATgc < 2:1732533/100‑1 (MQ=255) ccGCTACCCCAAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATgc > 2:787050/1‑100 (MQ=255) gCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATgcgc < 1:1734150/100‑1 (MQ=255) cccccAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATGCGCAAtt < 1:711582/100‑1 (MQ=255) ccccAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATGCGCAATtg < 1:122934/100‑1 (MQ=255) ccccAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATGCGCAATtg < 2:1032669/100‑1 (MQ=255) ccccAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATGCGCAATtg < 1:902680/100‑1 (MQ=255) ccccAAGACCCGAAATCAACACCACGACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATGCGCAAtt < 1:566741/99‑1 (MQ=255) ccAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATGCGCAATtgtg < 2:1177023/100‑1 (MQ=255) | GTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACCCGAAATCAACACCACTACGGGCGCATCAGCATAAGGGGGAGGTGAGAGTGAAAGTTTCATCGCGGCCTCACTTGGCGATATGCGCAATTGTG > NC_000913/1071601‑1071797 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |