Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,279,021 G→A A247V (GCG→GTG)  bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,279,0210GA95.1% 263.2 / 2.9 82A247V (GCG→GTG) bcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (41/37);  total (44/38)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGA  >  NC_000913/2278929‑2279117
                                                                                            |                                                                                                
cgcCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTg                                                                                           >  2:1395865/1‑100 (MQ=255)
  ccAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGt                                                                                         <  2:892216/100‑1 (MQ=255)
    aaTGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTa                                                                                       <  1:1584318/100‑1 (MQ=255)
    aaTGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTa                                                                                       <  1:295822/100‑1 (MQ=255)
    aaTGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTa                                                                                       <  1:1623092/100‑1 (MQ=255)
     aTGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTaa                                                                                      >  2:975523/1‑100 (MQ=255)
     aTGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTaa                                                                                      <  2:342771/100‑1 (MQ=255)
       gcggcgGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAAtt                                                                                    >  2:535820/1‑100 (MQ=255)
        cggcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAAttt                                                                                   >  2:1384438/1‑100 (MQ=255)
         ggcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTc                                                                                  >  1:1006346/1‑100 (MQ=255)
          gcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCa                                                                                 <  1:1320448/100‑1 (MQ=255)
          gcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCa                                                                                 <  2:860014/100‑1 (MQ=255)
          gcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCa                                                                                 <  2:127284/100‑1 (MQ=255)
           cggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCaa                                                                                <  2:798174/100‑1 (MQ=255)
            ggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAGGTTTTCCGGCACGACGTGGTTAATTTCAat                                                                               <  1:1526933/100‑1 (MQ=255)
            ggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAat                                                                               <  1:1006388/100‑1 (MQ=255)
               cGAAGCGGCTGTTAATGTTGGTCATCACGAACAGAAAGACAATGTTTAGCGCAAAGTAAGAACCAAAGTTTTCCGGCACGACGTTGTTAATTTCAAtata                                                                            <  1:1646879/100‑1 (MQ=255)
               cGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAAtata                                                                            >  1:811976/1‑100 (MQ=255)
               cGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAAtata                                                                            >  1:1783264/1‑100 (MQ=255)
                   gCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaaca                                                                        <  1:452974/100‑1 (MQ=255)
                      gCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaacaaac                                                                     <  2:536172/100‑1 (MQ=255)
                            aaaGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCg                                                               >  2:1618837/1‑100 (MQ=255)
                            aaaGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCg                                                               >  1:12456/1‑100 (MQ=255)
                              aGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgc                                                             >  2:1119326/1‑100 (MQ=255)
                                  ggTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCtt                                                         <  1:1188545/100‑1 (MQ=255)
                                   gTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTa                                                        <  1:1317770/100‑1 (MQ=255)
                                    tcatcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTaa                                                       >  1:829176/1‑100 (MQ=255)
                                     catcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAg                                                      >  1:1211779/1‑100 (MQ=255)
                                     catcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAg                                                      >  2:1695864/1‑100 (MQ=255)
                                      atcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGa                                                     <  1:543211/100‑1 (MQ=255)
                                       tcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGaa                                                    >  1:295430/1‑100 (MQ=255)
                                         aCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTTGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATg                                                  <  1:1510484/100‑1 (MQ=255)
                                         aCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATg                                                  >  1:1447826/1‑100 (MQ=255)
                                         aCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATg                                                  <  1:975522/100‑1 (MQ=255)
                                           gAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgag                                                <  1:1710873/100‑1 (MQ=255)
                                           gAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgag                                                <  1:117087/100‑1 (MQ=255)
                                            aaCAGAAGAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCGATATAAACAAACGGTCCGGCGCTTAAGAATgaga                                               >  1:1025780/1‑100 (MQ=255)
                                            aaCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgaga                                               <  2:1518002/100‑1 (MQ=255)
                                            aaCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgaga                                               >  1:1834010/1‑100 (MQ=255)
                                               aGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACa                                            >  2:610654/1‑100 (MQ=255)
                                                gAAAAACTATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACAt                                           >  2:1728640/1‑100 (MQ=255)
                                                gAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACAt                                           >  1:1505165/1‑100 (MQ=255)
                                                   aaaCAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATccc                                        >  2:650825/1‑100 (MQ=255)
                                                      cAATGTTTAGCGCAAAGTAATATCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGc                                     <  2:340503/100‑1 (MQ=255)
                                                      cAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGc                                     <  2:1783275/100‑1 (MQ=255)
                                                      cAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGc                                     <  2:295432/100‑1 (MQ=255)
                                                       aaTGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCa                                    >  2:312824/1‑100 (MQ=255)
                                                       aaTGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCa                                    <  1:310514/100‑1 (MQ=255)
                                                        aTGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCaa                                   <  2:125351/100‑1 (MQ=255)
                                                         tGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCaaa                                  >  2:1570341/1‑100 (MQ=255)
                                                         tGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCaaa                                  >  2:237278/1‑100 (MQ=255)
                                                          gTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAg                                 >  2:840061/1‑100 (MQ=255)
                                                             tAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTg                              <  1:1728629/100‑1 (MQ=255)
                                                             tAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTg                              <  1:367709/100‑1 (MQ=255)
                                                                 gcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTAAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCACCGCAAAGCTGAAAc                          >  1:1158141/1‑100 (MQ=255)
                                                                  cAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAAcc                         >  1:599805/1‑100 (MQ=255)
                                                                     aGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACt                      <  2:485814/100‑1 (MQ=255)
                                                                     aGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACt                      <  1:1682670/100‑1 (MQ=255)
                                                                     aGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGATTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACt                      <  1:1039954/100‑1 (MQ=255)
                                                                      gTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTg                     >  2:583160/1‑100 (MQ=255)
                                                                      gTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTg                     >  1:1370107/1‑100 (MQ=255)
                                                                      gTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTg                     >  2:27617/1‑100 (MQ=255)
                                                                      gTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTg                     >  2:258345/1‑100 (MQ=255)
                                                                      gTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTg                     >  2:1107909/1‑100 (MQ=255)
                                                                       taataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTgg                    <  2:1362786/100‑1 (MQ=255)
                                                                        aataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAGGCTGAAACCACTGGc                   >  2:1909983/1‑100 (MQ=255)
                                                                        aataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGc                   <  1:1282377/100‑1 (MQ=255)
                                                                         ataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCa                  >  1:1183927/1‑100 (MQ=255)
                                                                          taaCCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGTGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCaa                 >  1:1379003/1‑100 (MQ=255)
                                                                           aaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAg                >  1:175936/1‑100 (MQ=255)
                                                                             ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              <  2:256380/100‑1 (MQ=255)
                                                                             ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              <  2:981325/100‑1 (MQ=255)
                                                                             ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              >  1:204768/1‑100 (MQ=255)
                                                                              cAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCAt             >  2:1854058/1‑100 (MQ=255)
                                                                               aaaGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATg            <  1:147823/100‑1 (MQ=255)
                                                                               aaaGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATg            >  2:1460134/1‑100 (MQ=255)
                                                                                aaGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGt           <  1:483870/100‑1 (MQ=255)
                                                                                   ttttCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGc        >  2:556867/1‑100 (MQ=255)
                                                                                   ttttCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGc        >  2:1594669/1‑100 (MQ=255)
                                                                                        cGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAgg   >  2:835470/1‑100 (MQ=255)
                                                                                         ggCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGa  >  1:804684/1‑100 (MQ=255)
                                                                                         ggCACGACGTGGTTAATATCAATATAAACAAACGGTCGGGCGCTTAAGAATGAGAACATCGCGGCAAAGCTGAAACCAATGGCAAGCATGTAGCTGAGGa  >  1:1373737/1‑100 (MQ=255)
                                                                                            |                                                                                                
CGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGA  >  NC_000913/2278929‑2279117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑