Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,564,525 G→A M1M (ATG→ATA) † yffS → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,564,5250GA88.9% 252.4 / 16.4 91M1M (ATG→ATA) †yffSCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (6/4);  new base A (47/33);  total (54/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGCA  >  NC_000913/2564428‑2564621
                                                                                                 |                                                                                                 
gtgATCTGCGTCAATATTCCCTCCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGTAGATAAAAATCATGAg                                                                                                 <  2:455214/100‑1 (MQ=255)
  gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGtt                                                                                               >  2:1395894/1‑100 (MQ=255)
  gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGtt                                                                                               >  2:1102017/1‑100 (MQ=255)
  gATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGtt                                                                                               >  1:566660/1‑100 (MQ=255)
    tCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTAc                                                                                            <  1:853223/100‑1 (MQ=255)
      tGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGa                                                                                          >  2:547680/1‑100 (MQ=255)
       gCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGaa                                                                                         <  1:1118174/100‑1 (MQ=255)
        cGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGaaa                                                                                        >  2:1893266/1‑100 (MQ=255)
        cGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCAGGAGATGGGAGATAAAAATCATAAGTTACGaaa                                                                                        >  1:1618686/1‑100 (MQ=255)
              tatTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCaaaa                                                                                  <  2:37443/100‑1 (MQ=255)
              tatTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCaaaa                                                                                  >  1:50769/1‑100 (MQ=255)
               atTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAt                                                                                 >  2:12860/1‑100 (MQ=255)
                ttCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAtt                                                                                >  1:1272431/1‑100 (MQ=255)
                ttCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAAtt                                                                                <  1:161808/100‑1 (MQ=255)
                ttCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAGTTGACGAAATCAAAAt                                                                                 >  1:159087/1‑99 (MQ=255)
                  cccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTg                                                                              >  1:1935901/1‑100 (MQ=255)
                  cccTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTg                                                                              >  1:928686/1‑100 (MQ=255)
                    cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGGAATCAAAATTTGCg                                                                            >  1:1322688/1‑100 (MQ=255)
                    cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCg                                                                            <  2:1887521/100‑1 (MQ=255)
                    cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCg                                                                            >  1:1229707/1‑100 (MQ=255)
                    cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCg                                                                            <  1:1102016/100‑1 (MQ=255)
                    cTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCg                                                                            <  2:1153686/100‑1 (MQ=255)
                     ttCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGa                                                                           <  1:1012669/100‑1 (MQ=255)
                       ccGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACa                                                                         <  2:1673920/100‑1 (MQ=255)
                         gTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATc                                                                       >  1:336652/1‑100 (MQ=255)
                           tACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCt                                                                     <  2:706360/100‑1 (MQ=255)
                             cGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCa                                                                   <  1:903109/100‑1 (MQ=255)
                               cTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCaaa                                                                 >  1:1147042/1‑100 (MQ=255)
                                 tACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGAAGGGAGATAAAAATCATAAGTTCCGAAATCAAAATTTGCGACATCATCAAAg                                                                >  1:873479/1‑99 (MQ=255)
                                   cccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg                                                             >  2:371599/1‑100 (MQ=255)
                                   cccTCCACCAATACGCGTGTTAACCTACCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcg                                                             >  2:1183355/1‑100 (MQ=255)
                                    ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcgc                                                            >  1:817284/1‑100 (MQ=255)
                                    ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcgc                                                            >  1:659909/1‑100 (MQ=255)
                                    ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcgc                                                            >  2:1142124/1‑100 (MQ=255)
                                    ccTCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGgcgc                                                            <  1:765580/100‑1 (MQ=255)
                                      tCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTg                                                          <  2:50769/100‑1 (MQ=255)
                                      tCCACCAATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTg                                                          >  2:1793078/1‑100 (MQ=255)
                                       ccaccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGc                                                         >  1:775817/1‑100 (MQ=255)
                                       ccaccaATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGc                                                         >  2:1273239/1‑100 (MQ=255)
                                          ccaATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGAt                                                      >  2:1934099/1‑100 (MQ=255)
                                           caATACGCGTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATg                                                     >  1:650028/1‑100 (MQ=255)
                                                cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAgg                                                >  2:1837354/1‑100 (MQ=255)
                                                cgcgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAg                                                 <  1:179626/99‑1 (MQ=255)
                                                cgcgTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAgg                                                >  1:73731/1‑100 (MQ=255)
                                                  cgTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGAc                                              <  1:979119/100‑1 (MQ=255)
                                                  cgTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGAc                                              <  2:1641434/100‑1 (MQ=255)
                                                  cgTGTTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGAc                                              >  1:1149763/1‑100 (MQ=255)
                                                   gtgtTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACa                                             >  2:627628/1‑100 (MQ=255)
                                                   gtgtTAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACa                                             <  1:346862/100‑1 (MQ=255)
                                                       tAACCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGAGATGGAAGGACAatat                                         >  1:1901371/1‑100 (MQ=255)
                                                        aaCCTCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAAtata                                        >  1:529475/1‑100 (MQ=255)
                                                            tCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg                                    <  2:1748969/100‑1 (MQ=255)
                                                            tCCCAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAggg                                    <  1:1395886/100‑1 (MQ=255)
                                                             cccAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATGTAAGGgc                                   >  1:753623/1‑100 (MQ=255)
                                                              ccAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcg                                  >  1:1173339/1‑100 (MQ=255)
                                                              ccAATGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcg                                  >  2:1279320/1‑100 (MQ=255)
                                                               cAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGgcgc                                 <  2:1238591/100‑1 (MQ=255)
                                                                aaTGGATTCTATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                                <  2:1057826/100‑1 (MQ=255)
                                                                aaTGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                                >  2:1610756/1‑100 (MQ=255)
                                                                aaTGGATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCt                                >  2:659447/1‑100 (MQ=255)
                                                                   ggATTCCATGAGATGGGAGATAAAAATCATAAGTGACGAAATCAAAATTTGCGCAAACCTCAAAGGCACTGCGATGGAAGGAGAATATAAGGgcgaaca                              >  1:1925103/1‑95 (MQ=255)
                                                                    gATTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAAc                            <  2:936532/100‑1 (MQ=255)
                                                                     aTTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACg                           <  2:1229711/100‑1 (MQ=255)
                                                                     aTTCCATGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACg                           >  1:410806/1‑100 (MQ=255)
                                                                        ccaTGAGATGGGTGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg                        >  1:1519214/3‑100 (MQ=255)
                                                                        ccaTGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgg                        >  2:430098/3‑100 (MQ=255)
                                                                          aTGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTgggg                      >  1:1210066/1‑100 (MQ=255)
                                                                           tGAGATGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGc                     <  2:327030/100‑1 (MQ=255)
                                                                             agaTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCa                   <  1:1070033/100‑1 (MQ=255)
                                                                              gaTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCaa                  >  2:798148/1‑100 (MQ=255)
                                                                               aTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCaaa                 <  1:340311/100‑1 (MQ=255)
                                                                               aTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCaaa                 >  2:1030580/1‑100 (MQ=255)
                                                                               aTGGGAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCaa                  >  1:108147/1‑99 (MQ=255)
                                                                                 gggAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATg               >  2:1124095/1‑100 (MQ=255)
                                                                                 gggAGATAAAAAGCATAAGTGACGAAATCAAAAGTGGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATg               <  2:601842/100‑1 (MQ=255)
                                                                                  ggAGATATAAATGACAAGTTACGAATTCAAAATCTGCTACATCCTCAAAGGCGCTGCAATAGAAGTATAATATAAGGACTAAAAACTTGGAGCCAAAtac              >  1:1337318/1‑98 (MQ=255)
                                                                                  ggAGATAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGc              <  1:311009/100‑1 (MQ=255)
                                                                                   gagaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCg             >  2:132480/1‑100 (MQ=255)
                                                                                     gaTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgag           >  1:1567397/1‑100 (MQ=255)
                                                                                     gaTAAAAAGCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgag           <  1:298360/100‑1 (MQ=255)
                                                                                      aTAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagg          <  2:928687/100‑1 (MQ=255)
                                                                                       tAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagga         >  1:1651994/1‑100 (MQ=255)
                                                                                       tAAAAATCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagga         >  1:1100790/1‑100 (MQ=255)
                                                                                        aaaaaTCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgaggag        <  1:712537/100‑1 (MQ=255)
                                                                                        aaaaaTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCgagaag        >  1:663701/1‑100 (MQ=255)
                                                                                          aaaTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGAt      <  2:601377/100‑1 (MQ=255)
                                                                                           aaTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATc     <  2:649642/100‑1 (MQ=255)
                                                                                            aTCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCg    <  1:1595710/100‑1 (MQ=255)
                                                                                             tCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGc   <  1:1684874/100‑1 (MQ=255)
                                                                                             tCATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGc   <  2:142040/100‑1 (MQ=255)
                                                                                              cATAAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGCa  >  2:1684884/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
GTGATCTGCGTCAATATTCCCTTCCGTTACGCTTACCCTCCACCAATACGCGTGTTAACCTCCCAATGGATTCTATGAGATGGGAGATAAAAATCATGAGTTACGAAATCAAAATTTGCGACATCCTCAAAGGCGCTGCGATGGAAGGACAATATAAGGGCGCTCAACGTGGGGCCAAATGCGAGGAGATCGCA  >  NC_000913/2564428‑2564621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: