| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,593,917 | G→A | A648V (GCG→GTG) | tmcA ← | elongator methionine tRNA (ac4C34) acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,593,917 | 0 | G | A | 96.3% | 266.6 / ‑0.7 | 82 | A648V (GCG→GTG) | tmcA | elongator methionine tRNA (ac4C34) acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (27/51); total (30/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTA > NC_000913/2593820‑2594013 | taCCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCAcc < 2:104859/99‑1 (MQ=255) ccATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTg > 1:1803060/1‑100 (MQ=255) ccATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTg > 2:454823/1‑100 (MQ=255) cATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTgc > 1:1153558/1‑100 (MQ=255) tGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTgcgc > 2:1115219/1‑100 (MQ=255) tGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTgcgc > 1:121428/1‑100 (MQ=255) gcgaTAAAATTGAAGCAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGcgcg < 1:504807/98‑1 (MQ=255) gagaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGcgcg < 1:504749/100‑1 (MQ=255) aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc < 1:454821/100‑1 (MQ=255) aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc > 1:1547275/1‑100 (MQ=255) tAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCct > 1:1875024/1‑100 (MQ=255) aaaaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCctc < 1:1120972/100‑1 (MQ=255) aaaaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCctc < 1:427875/100‑1 (MQ=255) aaaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCctct > 2:1440716/1‑100 (MQ=255) ttGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGCTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTcc < 1:1366316/100‑1 (MQ=255) ttGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTcc < 2:561164/100‑1 (MQ=255) ttGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTcc > 2:735485/1‑100 (MQ=255) tGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTccc < 2:501603/100‑1 (MQ=255) gAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGa > 2:1018774/1‑100 (MQ=255) gAGTCAGCGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGa < 2:467318/100‑1 (MQ=255) gTCAATGAAACAATTGCCATTGCGTTATGCTATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGAc < 2:362179/99‑1 (MQ=255) tCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACgg < 2:621962/100‑1 (MQ=255) tCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACgg < 2:458291/100‑1 (MQ=255) cAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGa > 1:494602/1‑100 (MQ=255) aGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAc > 2:1504165/1‑100 (MQ=255) gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAcc < 2:1933900/100‑1 (MQ=255) gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAcc < 1:1147768/100‑1 (MQ=255) tGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCa < 2:1444283/100‑1 (MQ=255) gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg > 1:1048453/1‑100 (MQ=255) gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg > 1:1944738/1‑100 (MQ=255) aataatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCCCCTGCGCGGCCTCTTCCCGCTGACGGAcc > 1:32924/1‑96 (MQ=255) aataatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa < 1:735484/100‑1 (MQ=255) ataatTGCCATTGCGTTATGCGATCGCGCAGACGCTGAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTaa > 2:1371773/1‑100 (MQ=255) taatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAc < 2:1069333/100‑1 (MQ=255) taatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAc < 1:1432674/100‑1 (MQ=255) ttGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATc < 2:906097/100‑1 (MQ=255) tGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCt < 2:1130736/100‑1 (MQ=255) tGCCATTGCGTTATGCGAACGCGCAGACGCTCAGTGAGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCt > 1:567824/1‑100 (MQ=255) ccATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTg < 2:1323879/100‑1 (MQ=255) ccATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTg > 2:1346569/1‑100 (MQ=255) cATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGc < 1:784470/100‑1 (MQ=255) cATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGc < 1:35902/100‑1 (MQ=255) aTTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCg < 2:1137740/100‑1 (MQ=255) ttGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCgg < 2:105542/100‑1 (MQ=255) tGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGc < 1:1255642/100‑1 (MQ=255) gTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTg < 1:1492069/100‑1 (MQ=255) ttATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGa > 1:137308/1‑100 (MQ=255) cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt > 1:424166/1‑100 (MQ=255) aTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTa < 2:1776086/100‑1 (MQ=255) aTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTa < 2:101508/100‑1 (MQ=255) aTCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTa > 1:783725/1‑100 (MQ=255) tCGCGGAGACGCACAGTGAGAACATGATTAAGGGCGAACAGAAACTGCGCGGCTTCTTGCCGGTGACGGACAAGTAACATCTTGCGGCCTGAAAGta < 1:265801/97‑2 (MQ=255) cgcgCAGACGCTCAGTGCGAACATCATTGAGTGCGAACAGCACCTGCGCGGCGTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg < 1:781476/100‑1 (MQ=255) cgcgCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg < 1:1098278/100‑1 (MQ=255) cgcgCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg > 1:1422103/1‑100 (MQ=255) cgcgCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAg < 2:1875036/100‑1 (MQ=255) gcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgtgt < 2:322829/100‑1 (MQ=255) cAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGtgtg > 1:723535/1‑100 (MQ=255) gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt < 2:272823/100‑1 (MQ=255) gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt < 2:1824381/100‑1 (MQ=255) gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt < 1:1224834/100‑1 (MQ=255) gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt < 1:1207431/100‑1 (MQ=255) gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt < 1:535932/100‑1 (MQ=255) gACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGt > 1:743918/1‑100 (MQ=255) aCGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTa < 1:1018773/100‑1 (MQ=255) cGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTAc > 1:154564/1‑100 (MQ=255) cGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTAc < 2:54459/100‑1 (MQ=255) cAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATaa > 2:1530544/1‑100 (MQ=255) gCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgc > 2:903662/1‑100 (MQ=255) aaCATCATTAAGTGCGAACAGCACCTGCGCGGCATCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcgct < 1:475700/100‑2 (MQ=255) aCATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCAt > 2:1778242/1‑100 (MQ=255) tcatTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAAGATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCAc < 1:1079557/100‑1 (MQ=255) tcatTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCAc < 2:1547285/100‑1 (MQ=255) ttAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTgg < 2:1153560/100‑1 (MQ=255) tAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGc > 2:1131093/1‑100 (MQ=255) gTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtt < 2:1389097/100‑1 (MQ=255) gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc < 2:1217028/100‑1 (MQ=255) gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc < 2:1214858/100‑1 (MQ=255) aaCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCt > 2:398181/1‑100 (MQ=255) aCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTg < 1:1923483/100‑1 (MQ=255) aCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTg < 1:819002/100‑1 (MQ=255) aGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTa < 2:245057/100‑1 (MQ=255) | GACCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGTA > NC_000913/2593820‑2594013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |