Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 232,196 G→T G91C (GGC→TGC)  yafE → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913232,1960GT93.1% 342.8 / 14.7 102G91C (GGC→TGC) yafEputative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (45/50);  total (48/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 102 total aligned reads displayed.

CGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACG  >  NC_000913/232099‑232292
                                                                                                 |                                                                                                
cGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGc                                                                                                <  1:1850187/100‑1 (MQ=255)
  aTAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCgg                                                                                              <  1:1853490/100‑1 (MQ=255)
   tAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGt                                                                                             >  2:1352059/1‑100 (MQ=255)
   tAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGt                                                                                             <  1:1491835/100‑1 (MQ=255)
    aaCGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTa                                                                                            >  1:1692343/1‑100 (MQ=255)
     aCGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAg                                                                                           <  2:1693969/100‑1 (MQ=255)
     aCGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAg                                                                                           >  2:652792/1‑100 (MQ=255)
     aCGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAg                                                                                           >  1:607080/1‑100 (MQ=255)
      cGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAgg                                                                                          >  2:1028598/1‑100 (MQ=255)
       gCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGc                                                                                         <  1:442922/100‑1 (MQ=255)
         aTTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTTCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTg                                                                                       >  2:486525/1‑100 (MQ=255)
          tttGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGa                                                                                      <  2:789545/100‑1 (MQ=255)
            tGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGAtt                                                                                    <  2:1888493/100‑1 (MQ=255)
            tGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGAtt                                                                                    >  2:548872/1‑100 (MQ=255)
            tGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGAtt                                                                                    <  2:1500529/100‑1 (MQ=255)
            tGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGAtt                                                                                    >  2:1031733/1‑100 (MQ=255)
              atatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgt                                                                                  <  2:41467/100‑1 (MQ=255)
              atatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgt                                                                                  <  2:235897/100‑1 (MQ=255)
               tatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGATAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgtg                                                                                 <  2:1789551/100‑1 (MQ=255)
               tatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgtg                                                                                 <  1:838722/100‑1 (MQ=255)
               tatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgtg                                                                                 >  1:1103225/1‑100 (MQ=255)
               tatTGGTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGGGCAGCACTGCGGTAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgtg                                                                                 >  1:88652/1‑100 (MQ=255)
                atTGTTATCAGCCGTTATTCTGCCCATCGCTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgtg                                                                                 <  1:561881/99‑1 (MQ=255)
                atTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGa                                                                                <  1:1532564/100‑1 (MQ=255)
                atTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGa                                                                                <  2:584164/100‑1 (MQ=255)
                  tGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATg                                                                              <  1:1782888/100‑1 (MQ=255)
                    ttATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGa                                                                            >  2:1504036/1‑100 (MQ=255)
                    ttATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGa                                                                            >  2:1504013/1‑100 (MQ=255)
                     tATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGAc                                                                           >  2:1707879/1‑100 (MQ=255)
                         aGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTaa                                                                       <  1:1667357/100‑1 (MQ=255)
                          gCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAAt                                                                      <  2:751710/100‑1 (MQ=255)
                          gCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAAt                                                                      <  2:1254094/100‑1 (MQ=255)
                          gCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAAt                                                                      <  1:1028597/100‑1 (MQ=255)
                           ccGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATg                                                                     <  1:1329340/100‑1 (MQ=255)
                           ccGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATg                                                                     <  1:162597/100‑1 (MQ=255)
                            cGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGt                                                                    >  1:1941921/1‑100 (MQ=255)
                            cGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGt                                                                    >  1:144699/1‑100 (MQ=255)
                             gTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGtc                                                                   >  2:880854/1‑100 (MQ=255)
                                   cTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCggg                                                             <  2:436207/100‑1 (MQ=255)
                                   cTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCggg                                                             <  2:1728865/100‑1 (MQ=255)
                                    tGCCCATCACTGGCATGATGTTGGTGCAGCACAGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGt                                                            >  2:1840214/1‑100 (MQ=255)
                                     gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTc                                                           >  2:845753/1‑100 (MQ=255)
                                     gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTc                                                           >  2:388108/1‑100 (MQ=255)
                                       ccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCAc                                                         >  1:143939/1‑100 (MQ=255)
                                        cATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCAcc                                                        >  2:1143635/1‑100 (MQ=255)
                                        cATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCAcc                                                        <  1:1286763/100‑1 (MQ=255)
                                         aTCACTGGCATGATGTTGGTGCAGCGCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCAccc                                                       <  1:173625/100‑1 (MQ=255)
                                               ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTgcg                                                 >  2:1750080/1‑100 (MQ=255)
                                               ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGCAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTgcg                                                 >  1:450776/1‑100 (MQ=255)
                                                 cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGcgcg                                               >  1:974647/1‑100 (MQ=255)
                                                 cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGcgcg                                               >  1:1698814/1‑100 (MQ=255)
                                                   tgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGAc                                             >  2:1336261/1‑100 (MQ=255)
                                                      tgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATc                                          >  1:15243/1‑100 (MQ=255)
                                                       gTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCt                                         <  1:116879/100‑1 (MQ=255)
                                                       gTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCt                                         >  1:1902830/1‑100 (MQ=255)
                                                       gTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCt                                         <  1:27251/100‑1 (MQ=255)
                                                        ttGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTg                                        <  2:450778/100‑1 (MQ=255)
                                                         tGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTgg                                       >  2:271571/1‑100 (MQ=255)
                                                          ggTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGt                                      >  1:1829365/1‑100 (MQ=255)
                                                             gcagcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTCGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTAc                                   >  2:1314975/1‑100 (MQ=255)
                                                             gcagcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTAc                                   >  2:22832/1‑100 (MQ=255)
                                                              cagcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACa                                  <  2:1641854/100‑1 (MQ=255)
                                                              cagcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACa                                  <  2:453220/100‑1 (MQ=255)
                                                                 caCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                               <  2:809773/100‑1 (MQ=255)
                                                                 caCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                               >  2:1398632/1‑100 (MQ=255)
                                                                 caCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                               <  2:1354927/100‑1 (MQ=255)
                                                                 caCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                               <  1:200982/100‑1 (MQ=255)
                                                                  aCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACg                              >  2:386440/1‑100 (MQ=255)
                                                                  aCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACg                              >  2:1302222/1‑100 (MQ=255)
                                                                   cTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACgg                             <  1:1840202/100‑1 (MQ=255)
                                                                    tGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                            <  2:15243/100‑1 (MQ=255)
                                                                     gCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                            >  2:1771626/1‑99 (MQ=255)
                                                                     gCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTa                           <  1:515994/100‑1 (MQ=255)
                                                                     gCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTa                           <  2:607082/100‑1 (MQ=255)
                                                                      cGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAg                          <  1:1225480/100‑1 (MQ=255)
                                                                       gagaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                         >  2:1112654/1‑100 (MQ=255)
                                                                       gagaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                         <  1:1394387/100‑1 (MQ=255)
                                                                        agaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGaa                        <  1:104711/100‑1 (MQ=255)
                                                                          aaGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGc                      <  2:262347/100‑1 (MQ=255)
                                                                          aaGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGc                      <  1:1600908/100‑1 (MQ=255)
                                                                          aaGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGc                      <  1:1906599/100‑1 (MQ=255)
                                                                           aGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCa                     <  2:1196783/100‑1 (MQ=255)
                                                                           aGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCa                     >  1:353036/1‑100 (MQ=255)
                                                                              gAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTa                  >  1:404526/1‑100 (MQ=255)
                                                                                aTAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcg                >  1:809287/1‑100 (MQ=255)
                                                                                 tAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgc               >  2:156140/1‑100 (MQ=255)
                                                                                    gATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGAt            <  2:101599/100‑1 (MQ=255)
                                                                                     atatTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATa           <  2:397409/100‑1 (MQ=255)
                                                                                      tatTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAc          >  1:1526652/1‑100 (MQ=255)
                                                                                       atTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAcc         >  2:1467276/1‑100 (MQ=255)
                                                                                       atTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAcc         <  2:795784/100‑1 (MQ=255)
                                                                                        ttGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGTGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACct        >  1:1369187/1‑100 (MQ=255)
                                                                                        ttGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAcc         >  2:507052/1‑99 (MQ=255)
                                                                                        ttGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACct        >  2:545638/1‑100 (MQ=255)
                                                                                          gAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctct      <  1:8158/100‑1 (MQ=255)
                                                                                            aaCCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCa    >  2:1670631/1‑100 (MQ=255)
                                                                                            aaCCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCAACCAGTGCGCGGCATCTGGTTACAGACGGTAGAAGCATTACGCGATAACTCTCa    >  1:566591/1‑100 (MQ=255)
                                                                                             aCCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCa    <  1:388106/99‑1 (MQ=255)
                                                                                             aCCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCAc   <  1:987341/100‑1 (MQ=255)
                                                                                              ccTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACg  <  1:1291574/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CGATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACG  >  NC_000913/232099‑232292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: