Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,041,028 G→A R15C (CGT→TGT)  sdhE ← flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,041,0280GA96.2% 251.6 / 0.2 82R15C (CGT→TGT) sdhEflavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (28/49);  total (31/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

AGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCC  >  NC_000913/3040931‑3041125
                                                                                                 |                                                                                                 
agcagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagc                                                                                                 >  1:1873402/1‑100 (MQ=255)
  cagACGAATAAAGATGCGTTTTTTTTTTTTTTTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagcgg                                                                                               >  1:1252836/1‑100 (MQ=255)
  cagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcagcgg                                                                                               <  1:682647/100‑1 (MQ=255)
    gACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCa                                                                                             <  2:945914/100‑1 (MQ=255)
       gAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGc                                                                                          <  2:44402/100‑1 (MQ=255)
        aaTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGcc                                                                                         <  2:141523/100‑1 (MQ=255)
        aaTAAAGATGCGTTTTTCGTCATAGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTAGTGCATAGCACCGCGGCATGc                                                                                          >  2:1936379/1‑99 (MQ=255)
         aTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGccc                                                                                        <  1:1055083/100‑1 (MQ=255)
         aTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGccc                                                                                        >  1:187618/1‑100 (MQ=255)
         aTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGccc                                                                                        >  2:410237/1‑100 (MQ=255)
             aGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatg                                                                                    <  2:1160687/100‑1 (MQ=255)
             aGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatg                                                                                    <  2:343789/100‑1 (MQ=255)
             aGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatg                                                                                    >  1:1241067/1‑100 (MQ=255)
               aTGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatgaa                                                                                  >  2:1400076/1‑100 (MQ=255)
                 gCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatgaatg                                                                                <  2:149526/100‑1 (MQ=255)
                 gCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatgaatg                                                                                <  2:509727/100‑1 (MQ=255)
                   gTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCg                                                                              <  1:618829/100‑1 (MQ=255)
                     ttttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCggg                                                                            <  2:391201/100‑1 (MQ=255)
                     ttttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCggg                                                                            <  2:1236702/100‑1 (MQ=255)
                      tttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGc                                                                           <  2:866943/100‑1 (MQ=255)
                            cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgtt                                                                     <  1:836252/100‑1 (MQ=255)
                            cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgtt                                                                     >  2:1523924/1‑100 (MQ=255)
                             aTCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGTGGGCTttgttg                                                                    <  2:1800587/100‑1 (MQ=255)
                              tCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTtgttgt                                                                   >  1:696999/1‑100 (MQ=255)
                               cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                  >  2:687555/1‑100 (MQ=255)
                                gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACCGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                  >  1:1612861/1‑99 (MQ=255)
                                gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa                                                                 <  1:410235/100‑1 (MQ=255)
                                gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa                                                                 <  1:335505/100‑1 (MQ=255)
                                  ttAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAAt                                                               <  1:322439/100‑1 (MQ=255)
                                       cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                          <  1:1610384/100‑1 (MQ=255)
                                       cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                          <  1:1610349/100‑1 (MQ=255)
                                        tGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                          <  2:800731/99‑1 (MQ=255)
                                          tCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtct                                                       <  2:463734/100‑1 (MQ=255)
                                           cGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtctt                                                      <  1:156331/100‑1 (MQ=255)
                                            gTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtcttc                                                     >  2:355376/1‑100 (MQ=255)
                                             tACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtcttct                                                    >  2:225034/1‑100 (MQ=255)
                                               cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                  >  2:920588/1‑100 (MQ=255)
                                               cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                  >  1:244842/1‑100 (MQ=255)
                                               cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                  <  1:1475060/100‑1 (MQ=255)
                                               cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcctc                                                  >  2:428119/1‑100 (MQ=255)
                                                tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc                                                 <  1:375401/100‑1 (MQ=255)
                                                tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc                                                 <  2:697000/100‑1 (MQ=255)
                                                  acgTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTg                                               <  1:1181694/98‑1 (MQ=255)
                                                   tGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGt                                              >  2:1882657/1‑100 (MQ=255)
                                                    gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                             >  1:898860/1‑100 (MQ=255)
                                                    gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                             <  1:1523916/100‑1 (MQ=255)
                                                     ttCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGCATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                             <  1:352052/99‑1 (MQ=255)
                                                     ttCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtct                                            >  1:1510185/1‑100 (MQ=255)
                                                      tCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtctc                                           <  2:244844/100‑1 (MQ=255)
                                                       cGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCa                                          >  2:358695/1‑100 (MQ=255)
                                                       cGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCa                                          >  1:429968/1‑100 (MQ=255)
                                                             aCGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGaaaa                                    <  2:699481/100‑1 (MQ=255)
                                                               ggCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATc                                  <  1:1097021/100‑1 (MQ=255)
                                                                gCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATcc                                 <  1:700321/100‑1 (MQ=255)
                                                                gCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATcc                                 >  1:322059/1‑100 (MQ=255)
                                                                gCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATcc                                 <  1:1225778/100‑1 (MQ=255)
                                                                gCATGATCGAAAGATCGAGTTCGCGCATACCACAGCGGCGTGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAAGcc                                 <  1:1225563/100‑1 (MQ=255)
                                                                 cATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCa                                >  2:580798/1‑100 (MQ=255)
                                                                  aTGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                               <  2:615719/100‑1 (MQ=255)
                                                                   tGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGt                              <  1:1533377/100‑1 (MQ=255)
                                                                    gATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                             >  2:183347/1‑100 (MQ=255)
                                                                    gATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                             >  2:1382716/1‑100 (MQ=255)
                                                                    gATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                             <  2:933574/100‑1 (MQ=255)
                                                                    gATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                             <  1:1036078/100‑1 (MQ=255)
                                                                        gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCCTCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                         >  1:1162454/1‑100 (MQ=255)
                                                                        gAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                         <  1:1616567/100‑1 (MQ=255)
                                                                          aaTATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGc                       <  2:946129/100‑1 (MQ=255)
                                                                            tatCGAGTTCGCGCATGCCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTa                     <  1:1851191/100‑1 (MQ=255)
                                                                             atCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTAt                    <  2:1261894/100‑1 (MQ=255)
                                                                               cGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTg                  <  2:287989/100‑1 (MQ=255)
                                                                               cGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTg                  >  2:621815/1‑100 (MQ=255)
                                                                                gAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGt                 >  2:1602929/1‑100 (MQ=255)
                                                                                 aGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTa                <  1:1877092/100‑1 (MQ=255)
                                                                                  gTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTaa               <  2:1252840/100‑1 (MQ=255)
                                                                                  gTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTaa               >  2:1609771/1‑100 (MQ=255)
                                                                                    tCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAAc              <  2:146021/99‑1 (MQ=255)
                                                                                    tCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTCGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg             <  1:1242022/100‑1 (MQ=255)
                                                                                      gcgcATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgtg           >  1:685178/1‑100 (MQ=255)
                                                                                         cATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGttt        <  1:687554/100‑1 (MQ=255)
                                                                                          aTACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGtttt       >  1:193036/1‑100 (MQ=255)
                                                                                           tACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCTCGAAAATCCAGTACCCGGCTATTGTAACGTGtttt       <  1:464878/99‑1 (MQ=255)
                                                                                           tACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGttttt      >  2:327576/1‑100 (MQ=255)
                                                                                            aCCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTc     <  1:621814/100‑1 (MQ=255)
                                                                                             ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg    >  1:1494216/1‑100 (MQ=255)
                                                                                             ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg    <  2:260208/100‑1 (MQ=255)
                                                                                              cacaGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGc   >  2:1036615/1‑100 (MQ=255)
                                                                                                cggcggCATGCCCAATGAATGCGGGCTTGGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAAGCCAGTACCCGGCTATTGTAACGTGTTTTTCGcc  <  1:1171477/99‑1 (MQ=255)
                                                                                                 |                                                                                                 
AGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCC  >  NC_000913/3040931‑3041125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑