Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,165,015 T→A D320V (GAT→GTT)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,165,0150TA97.1% 231.6 / ‑3.7 70D320V (GAT→GTT) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (38/30);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTC  >  NC_000913/3164918‑3165112
                                                                                                 |                                                                                                 
cacCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACg                                                                                                 >  2:950386/1‑100 (MQ=255)
cacCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACg                                                                                                 >  2:289275/1‑100 (MQ=255)
  ccAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGCTCATATTAAGGTTAATACGATAGCTCTTTTCCAAAACgg                                                                                                >  1:798847/1‑99 (MQ=255)
  ccAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggt                                                                                               >  2:1202051/1‑100 (MQ=255)
   cAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGTACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACgga                                                                                               >  1:1180756/1‑98 (MQ=255)
    aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtgg                                                                                             <  2:572976/100‑1 (MQ=255)
      ccATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTa                                                                                           <  1:642118/100‑1 (MQ=255)
       cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGCTGGTAg                                                                                          >  1:834514/1‑100 (MQ=255)
            ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTCAGGTTAATACGATCGCTCTTTTCCAGAACGGTGGTAGcca                                                                                       >  1:557061/1‑96 (MQ=255)
                agGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggt                                                                                 <  2:1861070/100‑1 (MQ=255)
                 ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                                <  1:535050/100‑1 (MQ=255)
                 ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCCTTTCCAGAACGATGGTAGCGAAGAgatg                                                                                >  1:521908/1‑100 (MQ=255)
                   aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                              <  2:1106745/100‑1 (MQ=255)
                   aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                              <  1:379284/100‑1 (MQ=255)
                      tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtca                                                                           <  2:1640863/100‑1 (MQ=255)
                      tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtca                                                                           >  2:1436761/1‑100 (MQ=255)
                       ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcat                                                                          <  2:379943/100‑1 (MQ=255)
                       ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGCTGGTtcat                                                                          >  1:545985/1‑100 (MQ=255)
                         agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatca                                                                        >  2:1220385/1‑100 (MQ=255)
                             ggTTTTTTACCTCCGGTCGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTCGTAGCGAAGAGGTGGTTCATCACCTg                                                                    >  1:267003/1‑100 (MQ=255)
                                 tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                                <  1:65422/100‑1 (MQ=255)
                                  ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGAt                                                                  <  2:1101975/97‑1 (MQ=255)
                                  ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                               <  2:475601/100‑1 (MQ=255)
                                    cACCGCCGGATGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                             >  1:48370/1‑100 (MQ=255)
                                      ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc                                                           >  2:864011/1‑100 (MQ=255)
                                                acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                                 >  2:30050/1‑100 (MQ=255)
                                                acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                                 >  1:875207/1‑100 (MQ=255)
                                                 ccATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTgg                                                <  1:289273/100‑1 (MQ=255)
                                                  cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                               <  2:1180758/100‑1 (MQ=255)
                                                   aTCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGaa                                              >  2:1552740/1‑100 (MQ=255)
                                                    tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAc                                             >  1:279144/1‑100 (MQ=255)
                                                      cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                           >  2:1701182/1‑100 (MQ=255)
                                                       aGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgcg                                          >  1:99661/1‑100 (MQ=255)
                                                       aGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgcg                                          <  1:836321/100‑1 (MQ=255)
                                                        gACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                         <  2:267005/100‑1 (MQ=255)
                                                         aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGc                                        <  1:926578/100‑1 (MQ=255)
                                                          ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                       <  2:1098201/100‑1 (MQ=255)
                                                           cGATCATATTAAGGTTAATACGATAGCTCTGTTTCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGTAACGCGGcac                                      >  1:1281248/1‑100 (MQ=255)
                                                            gATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcaca                                     >  2:106712/1‑100 (MQ=255)
                                                            gATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcaca                                     >  2:551074/1‑100 (MQ=255)
                                                                atatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCa                                 >  2:1351458/1‑100 (MQ=255)
                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                                >  2:496428/1‑100 (MQ=255)
                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                                >  2:196685/1‑100 (MQ=255)
                                                                   ttAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCa                              >  1:418819/1‑100 (MQ=255)
                                                                       ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                          >  2:531702/1‑100 (MQ=255)
                                                                       ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                          <  1:714525/100‑1 (MQ=255)
                                                                        gTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgg                         >  2:1574522/1‑100 (MQ=255)
                                                                         ctAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggg                        <  2:965651/99‑1 (MQ=255)
                                                                          tAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggt                       <  1:648611/100‑1 (MQ=255)
                                                                          tAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggt                       >  1:545415/1‑100 (MQ=255)
                                                                          tAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggg                        <  1:1880151/99‑1 (MQ=255)
                                                                           aaTACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtc                      >  2:937737/1‑100 (MQ=255)
                                                                           aaTACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggag                      >  1:965650/1‑98 (MQ=255)
                                                                            aTACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATTACCAGGcgggtcg                     <  1:1202678/100‑1 (MQ=255)
                                                                              aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGCACGCGGCACAATCACCAGGcgggtcggg                   <  1:675008/100‑1 (MQ=255)
                                                                              aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                   <  2:1442520/100‑1 (MQ=255)
                                                                                gATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGtt                 >  1:282555/1‑100 (MQ=255)
                                                                                  tAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCAGGTCGGGTtct               >  1:104186/1‑100 (MQ=255)
                                                                                  tAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACAAAACGGGTCGGGTtc                >  1:1580137/1‑99 (MQ=255)
                                                                                    gCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg             >  1:1580261/1‑100 (MQ=255)
                                                                                       ctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg          >  2:1114144/1‑100 (MQ=255)
                                                                                       ctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg          <  2:374406/100‑1 (MQ=255)
                                                                                        ttttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGt         <  1:1291605/100‑1 (MQ=255)
                                                                                          ttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCa       <  1:496426/100‑1 (MQ=255)
                                                                                          ttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCa       >  2:1376147/1‑100 (MQ=255)
                                                                                           tCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAg      <  2:1027928/100‑1 (MQ=255)
                                                                                            ccAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGa     <  2:752545/100‑1 (MQ=255)
                                                                                             cAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt    >  2:295179/1‑100 (MQ=255)
                                                                                             cAGAACGGTGGTAGCGAAGAGGTGGTTCATCAACTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAg      <  1:96930/98‑1 (MQ=255)
                                                                                               gATCGGTCGTAGCGAAGAGGTGGTTCATGACCTGATCCATCTCGTCGCGGTTGGAACGCGTCACAACCACCAGGCTGGTCGGGTTCGCGTGGTCAGACTc  <  1:264743/100‑1 (MQ=255)
                                                                                                 |                                                                                                 
CACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTC  >  NC_000913/3164918‑3165112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑