Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,477,434 T→C intergenic (‑15/‑206) fkpA ← / → slyX FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,4340TC98.8% 309.7 / ‑4.6 86intergenic (‑15/‑206)fkpA/slyXFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (46/39);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATT  >  NC_000913/3477341‑3477530
                                                                                             |                                                                                                
cagcagCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGc                                                                                            >  1:1402732/1‑100 (MQ=255)
 agcagcAAAAGTGATTGGTGCATGCCGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGTTTTCATCCATATCTCCAGGACCGGGCCa                                                                                           >  1:1537865/1‑100 (MQ=255)
 agcagcAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCa                                                                                           >  1:1041048/1‑100 (MQ=255)
  gcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAt                                                                                          >  2:1856093/1‑100 (MQ=255)
  gcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAt                                                                                          >  2:719630/1‑100 (MQ=255)
  gcagcaAAAGTGACTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAt                                                                                          >  2:1306715/1‑100 (MQ=255)
   cagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtc                                                                                         <  2:1740098/100‑1 (MQ=255)
     gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                       >  2:1775003/1‑100 (MQ=255)
     gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                       >  1:1894733/1‑100 (MQ=255)
     gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                       >  1:1024728/1‑100 (MQ=255)
     gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGGCGCCAGCAGCGTTACTTTAAACCGTGATTTCATGCATATCTCCAGGACCGGGGCCtctc                                                                                       >  1:1816913/1‑100 (MQ=255)
      caAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCa                                                                                      >  2:491016/1‑100 (MQ=255)
       aaaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAc                                                                                     >  1:1717528/1‑100 (MQ=255)
            tGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAg                                                                                <  2:1652911/100‑1 (MQ=255)
            tGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAg                                                                                <  1:1027144/100‑1 (MQ=255)
             gATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAgg                                                                               >  2:259907/1‑100 (MQ=255)
                 ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                           >  2:1799443/1‑100 (MQ=255)
                 ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                           >  2:1724730/1‑100 (MQ=255)
                 ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                           >  2:1275506/1‑100 (MQ=255)
                    gcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACt                                                                        <  1:407131/100‑1 (MQ=255)
                    gcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACt                                                                        <  1:559576/100‑1 (MQ=255)
                     catgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACta                                                                       >  1:328301/1‑100 (MQ=255)
                       tgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtatt                                                                     >  2:816373/1‑100 (MQ=255)
                         caGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtattat                                                                   <  2:1902557/100‑1 (MQ=255)
                         caGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCAAGGACCGGGGCATCTCACCCCAGGGTTAACtattat                                                                   >  2:1364585/1‑100 (MQ=255)
                          aGGGCGACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGTTTTCATCTATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtattan                                                                   <  2:673202/99‑2 (MQ=255)
                          aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                  <  2:515899/100‑1 (MQ=255)
                             gCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGa                                                               <  2:1448882/100‑1 (MQ=255)
                                aCGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAgg                                                            <  1:285027/100‑1 (MQ=255)
                                 cGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAggg                                                           <  2:1289243/100‑1 (MQ=255)
                                   gCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTa                                                         >  2:292455/1‑100 (MQ=255)
                                   gCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTa                                                         >  1:629065/1‑100 (MQ=255)
                                    ccATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTAc                                                        >  1:133029/1‑100 (MQ=255)
                                    ccATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAAATATTATCAGAAGGGTAc                                                        >  2:671306/1‑100 (MQ=255)
                                      aTTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACta                                                      <  1:210382/100‑1 (MQ=255)
                                      aTTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACta                                                      >  2:376461/1‑100 (MQ=255)
                                       ttGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACtat                                                     <  1:454063/100‑1 (MQ=255)
                                         gtgGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATaa                                                   >  2:859827/1‑100 (MQ=255)
                                           ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 <  1:1009350/100‑1 (MQ=255)
                                           ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 <  1:262690/100‑1 (MQ=255)
                                           ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 <  1:54845/100‑1 (MQ=255)
                                           ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 >  1:436989/1‑100 (MQ=255)
                                           ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCAGCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 <  1:670534/100‑1 (MQ=255)
                                            gTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGc                                                >  1:1525089/1‑100 (MQ=255)
                                            gTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGc                                                >  2:341408/1‑100 (MQ=255)
                                                ccAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                            <  1:685185/100‑1 (MQ=255)
                                                 cagcagCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGtttt                                           >  1:314902/1‑98 (MQ=255)
                                                 cagcagCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGt                                           >  2:1736190/1‑100 (MQ=255)
                                                 cagcagCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGt                                           >  1:478467/1‑100 (MQ=255)
                                                  agcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                            <  2:638745/98‑1 (MQ=255)
                                                  agcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc                                          <  1:1007093/100‑1 (MQ=255)
                                                    cagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGa                                        <  2:436991/100‑1 (MQ=255)
                                                      gtgTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  1:1182310/98‑1 (MQ=255)
                                                      gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  2:783344/100‑1 (MQ=255)
                                                      gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  2:4704/100‑1 (MQ=255)
                                                      gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  2:1041049/100‑1 (MQ=255)
                                                       cGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACa                                     <  2:1768225/100‑1 (MQ=255)
                                                         ttACTTTAAACAGTTATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACaaa                                   <  2:159836/100‑1 (MQ=255)
                                                           aCTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                 >  1:431523/1‑100 (MQ=255)
                                                           aCTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                 >  2:1672041/1‑100 (MQ=255)
                                                            cTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                <  1:1515247/100‑1 (MQ=255)
                                                             tttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCta                               <  2:1071422/100‑1 (MQ=255)
                                                               tAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCCGAAGGGTACTATAAAGCGTTGTCGAACAAATCtaca                             >  1:1051473/1‑100 (MQ=255)
                                                               tAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtaca                             <  1:719629/100‑1 (MQ=255)
                                                                 aaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacata                           >  2:60483/1‑100 (MQ=255)
                                                                   cAGTGATTTCATCCATATCTCCAGGTCCGATGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacatac                          <  1:1669289/99‑1 (MQ=255)
                                                                   cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                         <  2:173884/100‑1 (MQ=255)
                                                                   cAGTGATTTCATCCATATCTCCAGGACCGGCGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAGTCtacataca                         >  1:1276613/1‑100 (MQ=255)
                                                                     gTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGa                       >  2:1692154/1‑100 (MQ=255)
                                                                     gTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGa                       >  1:174673/1‑100 (MQ=255)
                                                                      tGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGac                      <  2:1024729/100‑1 (MQ=255)
                                                                        aTTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGacac                    <  2:1327465/100‑1 (MQ=255)
                                                                         tttCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACg                   <  2:1221910/100‑1 (MQ=255)
                                                                             aTCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCt               >  2:1927277/1‑100 (MQ=255)
                                                                                cATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt            >  1:263695/1‑100 (MQ=255)
                                                                                cATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt            >  2:1652748/1‑100 (MQ=255)
                                                                                 atatCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatta           <  2:372225/100‑1 (MQ=255)
                                                                                   atCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatc         >  2:659155/1‑100 (MQ=255)
                                                                                      tcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc      <  1:259905/100‑1 (MQ=255)
                                                                                       ccAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTGTCATCt     <  1:652673/100‑1 (MQ=255)
                                                                                       ccAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCt     >  1:763099/1‑100 (MQ=255)
                                                                                        cAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTa    <  1:1672031/100‑1 (MQ=255)
                                                                                        cAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTa    <  1:1211492/100‑1 (MQ=255)
                                                                                        cAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTa    >  1:1910201/1‑100 (MQ=255)
                                                                                         aGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTAt   >  1:1850310/1‑100 (MQ=255)
                                                                                          ggACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTAtt  >  2:20073/1‑100 (MQ=255)
                                                                                             |                                                                                                
CAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATT  >  NC_000913/3477341‑3477530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑