| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,541,962 | T→G | I519S (ATC→AGC) | feoB → | ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,541,962 | 0 | T | G | 90.7% | 249.9 / 14.6 | 75 | I519S (ATC→AGC) | feoB | ferrous iron transporter protein B and GTP‑binding protein; membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (35/33); total (38/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTC > NC_000913/3541866‑3542056 | tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGagcat < 2:1530528/100‑1 (MQ=255)tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGagcat > 1:1734311/1‑100 (MQ=255) gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGagcatcatc > 1:866171/1‑100 (MQ=255) ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCg < 1:831354/100‑1 (MQ=255) ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCg < 1:478042/100‑1 (MQ=255) tGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGt > 2:762670/1‑100 (MQ=255) gCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTc < 1:1007274/100‑1 (MQ=255) gCCGGTCGATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTc > 2:1023079/1‑100 (MQ=255) ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCa < 2:876108/100‑1 (MQ=255) cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAg < 1:1772985/100‑1 (MQ=255) cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAg < 1:1705726/100‑1 (MQ=255) cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTCCAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAg > 1:14282/1‑100 (MQ=255) tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCAt < 1:636439/100‑1 (MQ=255) cTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtt > 2:437714/1‑100 (MQ=255) ntCATGTACCACACGTTAAAAGACTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTCCGTGCTGGTAAAGTGGGCATCATCGTCAGCAtttt > 1:909783/2‑100 (MQ=255) aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtttt > 2:524339/1‑100 (MQ=255) aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtttt < 2:845818/100‑1 (MQ=255) aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtttt > 2:729321/1‑100 (MQ=255) aTGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCt > 2:1192601/1‑100 (MQ=255) tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTg < 1:119972/100‑1 (MQ=255) tACCAGACGTTAAAGGCATCTTTATCTTGACTTGGCAGCGTCTGAAAGGCTTCGTTCTGTGTGCTGGTAAAGTGAGCATCATCTTCCGCATTTGCCTgn < 1:1159449/99‑2 (MQ=255) aCCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgc > 1:267031/1‑100 (MQ=255) ccACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgcg > 1:12679/1‑100 (MQ=255) cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgcgc > 2:865770/1‑100 (MQ=255) acacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCt > 1:790226/1‑100 (MQ=255) acacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCt < 1:1777496/100‑1 (MQ=255) cacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCtt > 1:1284797/1‑100 (MQ=255) cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc > 2:243881/1‑100 (MQ=255) cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc < 1:92867/100‑1 (MQ=255) cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc < 1:14247/100‑1 (MQ=255) cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc < 1:1580062/100‑1 (MQ=255) gTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCa < 2:1137915/100‑1 (MQ=255) gTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCa < 1:1811767/100‑1 (MQ=255) ttAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCaa > 2:1252735/1‑100 (MQ=255) ttAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCaa > 1:1706903/1‑100 (MQ=255) tAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAAc < 2:1619851/100‑1 (MQ=255) aaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAg > 1:339608/1‑100 (MQ=255) aaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAg < 1:1221274/100‑1 (MQ=255) aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGc < 1:60446/100‑1 (MQ=255) gCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCtt > 1:1385995/1‑100 (MQ=255) tGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTtctc < 1:566512/100‑1 (MQ=255) gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCg > 2:1615492/1‑100 (MQ=255) aTTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGc < 1:602453/100‑1 (MQ=255) ttATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCt > 2:1004088/1‑100 (MQ=255) aTCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGa > 2:1048500/1‑100 (MQ=255) cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCg < 1:1458299/100‑1 (MQ=255) aGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCgg < 2:163405/100‑1 (MQ=255) gACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCggg < 1:864789/100‑1 (MQ=255) ccTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGaa > 2:1839265/1‑100 (MQ=255) ccTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGaa > 2:425783/1‑100 (MQ=255) gCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAt < 1:1846701/99‑1 (MQ=255) cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcg > 1:1172729/1‑100 (MQ=255) cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcg < 2:35058/100‑1 (MQ=255) aGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgt > 2:437386/1‑100 (MQ=255) gCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtc < 1:1107654/100‑1 (MQ=255) cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtcg < 2:866172/100‑1 (MQ=255) cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtcg < 1:1176217/100‑1 (MQ=255) gTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGa > 2:1302878/1‑100 (MQ=255) tCTGAAAGGCTTCGTTCTGCGTGCTCGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGAt < 2:530400/100‑1 (MQ=255) cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATa > 2:1801779/1‑100 (MQ=255) tGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATaa < 1:1252731/100‑1 (MQ=255) tGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATaa > 1:1447672/1‑100 (MQ=255) gAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAAc > 1:1524001/1‑100 (MQ=255) aGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATc < 2:209751/100‑1 (MQ=255) tGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgc < 2:1677492/100‑1 (MQ=255) gCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgcg > 1:961818/1‑100 (MQ=255) gCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgcg > 2:1684386/1‑100 (MQ=255) gCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgcg > 1:961811/1‑100 (MQ=255) tGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGgcgc > 1:325610/1‑98 (MQ=255) gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg < 1:1801768/100‑1 (MQ=255) gCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg < 1:76083/100‑1 (MQ=255) gCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg < 1:762669/100‑1 (MQ=255) ggTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgt > 2:1939626/1‑100 (MQ=255) ggTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgt > 2:1939478/1‑100 (MQ=255) aaaGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg > 1:1619003/1‑100 (MQ=255) aaaGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg > 1:507750/1‑100 (MQ=255) aaaGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg < 2:1447680/100‑1 (MQ=255) aGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgtc > 2:861127/1‑100 (MQ=255) | TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTC > NC_000913/3541866‑3542056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |