| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,622,796 | T→G | L1202R (CTG→CGG) | rhsB → | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,622,796 | 0 | T | G | 94.1% | 168.5 / 3.7 | 68 | L1202R (CTG→CGG) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base G (39/25); total (42/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATA > NC_000913/3622699‑3622892 | tatGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGt < 2:1562938/100‑1 (MQ=38) gatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAtt < 2:1168712/100‑1 (MQ=18) atgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTa < 1:1892552/100‑1 (MQ=18) tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTAc > 2:93300/1‑100 (MQ=18) tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTAc > 2:510661/1‑100 (MQ=17) aTGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAAc < 2:1529877/100‑1 (MQ=18) aTGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAAc < 2:1530020/100‑1 (MQ=18) ggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGcc < 1:391021/100‑1 (MQ=18) aaCCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCAcc < 1:1539421/100‑1 (MQ=18) aaCCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCAcc > 1:777584/1‑100 (MQ=18) tgaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtatta > 2:1646390/1‑100 (MQ=18) gaatgaaGGGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtattat > 1:1728743/1‑100 (MQ=255) gaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtattat > 2:485370/1‑100 (MQ=18) atgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGa > 2:1896193/1‑100 (MQ=18) gaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAcc > 2:1431741/1‑100 (MQ=18) aaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAccc < 2:1386319/100‑1 (MQ=18) agagAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATAATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAccc > 2:720670/1‑99 (MQ=255) gagaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGc > 1:607880/1‑100 (MQ=18) gaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTg > 2:1108576/1‑100 (MQ=18) gaACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTg > 1:571321/1‑100 (MQ=18) cccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAg > 1:1094432/1‑100 (MQ=33) cccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAg > 2:1924285/1‑100 (MQ=18) gCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAgggg < 1:215381/100‑1 (MQ=18) gCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAgggg > 1:225839/1‑100 (MQ=18) cATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGc < 2:1625753/100‑1 (MQ=18) aTCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCg < 2:1485348/100‑1 (MQ=18) gCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGatat > 2:438193/1‑100 (MQ=18) gCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGatat > 1:1406775/1‑100 (MQ=17) tGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGatatat > 2:692630/1‑100 (MQ=18) gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGatatat > 2:1377520/1‑99 (MQ=18) gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATc < 1:454037/100‑1 (MQ=17) gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATc > 2:1095015/1‑100 (MQ=18) gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATc < 2:1032655/100‑1 (MQ=17) cagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTc > 2:387884/1‑100 (MQ=18) cagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTc < 1:1414589/100‑1 (MQ=18) agcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCa < 2:1437116/100‑1 (MQ=18) gcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAg > 1:1557454/1‑100 (MQ=18) tATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGAt > 2:774616/1‑100 (MQ=18) tATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGAt > 2:462831/1‑100 (MQ=18) tATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGAt > 2:1417301/1‑100 (MQ=18) ccGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCg > 2:859144/1‑100 (MQ=17) ccGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCg < 1:692629/100‑1 (MQ=18) ggCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGAt < 1:297526/100‑1 (MQ=14) gCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGa > 1:979345/1‑100 (MQ=11) gCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGa > 1:249042/1‑100 (MQ=11) ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGAc < 2:1358408/100‑1 (MQ=14) ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGAc > 2:125211/1‑100 (MQ=14) ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGAc < 2:607882/100‑1 (MQ=14) cGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACt > 2:1654138/1‑100 (MQ=32) gcagcaGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAg < 1:1187423/100‑1 (MQ=35) gcagcaGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGaan < 2:1761921/100‑2 (MQ=18) agTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggggg < 2:683920/100‑1 (MQ=255) agTATGATGAGGAGTCCGGCCGGTATAACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggggg > 2:1186780/1‑100 (MQ=255) gTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGa < 1:1924272/100‑1 (MQ=255) tgatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGAGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATga > 1:1070629/1‑99 (MQ=255) gatgaGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACCGAAGGGGGGATGGa > 1:549553/1‑100 (MQ=25) tgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAc > 2:17144/1‑100 (MQ=255) gaggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAcc > 1:682371/1‑100 (MQ=255) gaggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAcc > 2:1274167/1‑100 (MQ=255) ggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTg > 1:1302545/1‑100 (MQ=255) ggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGACATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTg > 2:1881173/1‑100 (MQ=255) gagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGt > 1:351367/1‑100 (MQ=255) gagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGt > 2:851874/1‑100 (MQ=255) agTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTa > 2:1839373/1‑100 (MQ=255) gTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTAt > 2:1756613/1‑100 (MQ=255) ccggccggTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGTATCCGCTTGGACTGCAGGGGGGATGGAACCTGTATgg > 1:1308045/1‑100 (MQ=17) ccggccggTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATgg > 1:1095392/1‑100 (MQ=255) ccggccggTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATgg > 2:1042933/1‑100 (MQ=255) ggCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGat < 1:112548/100‑1 (MQ=255) ggCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGat < 1:972128/100‑1 (MQ=255) gCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGata < 2:443622/100‑1 (MQ=255) | TATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATA > NC_000913/3622699‑3622892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |