Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 281,581 G→A R135W (CGG→TGG)  yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,5810GA95.4% 274.9 / 3.1 87R135W (CGG→TGG) yagACP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (43/40);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

CAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTG  >  NC_000913/281486‑281677
                                                                                               |                                                                                                
cagcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGccc                                                                                              >  1:76673/1‑100 (MQ=255)
 agcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCg                                                                                             <  2:646869/100‑1 (MQ=255)
 agcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCg                                                                                             >  1:148542/1‑100 (MQ=255)
  gcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGt                                                                                            >  1:1710675/1‑100 (MQ=255)
    agGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTgg                                                                                          <  2:121504/100‑1 (MQ=255)
     gggTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGc                                                                                         <  2:851754/100‑1 (MQ=255)
     gggTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGGTCGAACCAGCCCGTGGc                                                                                         >  1:520355/1‑100 (MQ=255)
      ggTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCg                                                                                        <  2:751134/100‑1 (MQ=255)
      ggTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAACCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCg                                                                                        <  2:1329258/100‑1 (MQ=255)
       gTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCgg                                                                                       <  2:590939/100‑1 (MQ=255)
        tGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCggg                                                                                      <  1:1489800/100‑1 (MQ=255)
        tGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCgcg                                                                                      <  2:1319794/100‑3 (MQ=255)
         gAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGa                                                                                     >  2:1849532/1‑100 (MQ=255)
           gCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAAt                                                                                   >  2:1616227/1‑100 (MQ=255)
           gCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAAt                                                                                   >  1:1775542/1‑100 (MQ=255)
           gCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAAt                                                                                   <  2:768956/100‑1 (MQ=255)
            cGGATGGCAGCGACAACCGCAAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATg                                                                                  <  1:99331/100‑1 (MQ=255)
             ggATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGc                                                                                 <  1:850160/100‑1 (MQ=255)
                tGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCg                                                                              <  2:711123/100‑1 (MQ=255)
                 ggCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCg                                                                              >  1:541667/1‑99 (MQ=255)
                   cAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTg                                                                           <  2:1092350/100‑1 (MQ=255)
                   cAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTg                                                                           <  1:458959/100‑1 (MQ=255)
                     gCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGaa                                                                         <  2:1853662/100‑1 (MQ=255)
                      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                        <  1:607946/100‑1 (MQ=255)
                      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                        <  2:136210/100‑1 (MQ=255)
                      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                        <  2:1072490/100‑1 (MQ=255)
                      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                        <  2:1767959/100‑1 (MQ=255)
                      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                        <  1:1003078/100‑1 (MQ=255)
                      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                        <  2:1920521/100‑1 (MQ=255)
                       gTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgc                                                                       >  2:1894077/1‑100 (MQ=255)
                        tCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgcg                                                                      >  2:707151/1‑100 (MQ=255)
                        tCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgcg                                                                      >  2:1540606/1‑100 (MQ=255)
                         ccACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgcgc                                                                     >  2:648567/1‑100 (MQ=255)
                         ccACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgcgc                                                                     >  1:611545/1‑100 (MQ=255)
                          cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGcc                                                                    >  2:353619/1‑100 (MQ=255)
                          cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGcc                                                                    >  1:1522679/1‑100 (MQ=255)
                          cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGCGGCGGGAATGCCCGGTGAAGCGcc                                                                    >  1:1066947/1‑100 (MQ=255)
                            ccGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCg                                                                  >  2:742040/1‑100 (MQ=255)
                                 aaaaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGTAATGCCCGGTGAAGCGCCCGgcagc                                                             <  2:1849189/100‑1 (MQ=255)
                                    aGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAgg                                                          >  1:300552/1‑100 (MQ=255)
                                    aGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAgg                                                          >  1:1151174/1‑100 (MQ=255)
                                      gAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGcc                                                        <  2:9380/100‑1 (MQ=255)
                                       aaaGTGGCCCTTAAAAGCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCa                                                       <  1:532104/100‑1 (MQ=255)
                                           tGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGTGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGc                                                   >  1:1038570/1‑100 (MQ=255)
                                              cccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                                <  1:1229575/100‑1 (MQ=255)
                                                 ttAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCa                                             >  1:463593/1‑100 (MQ=255)
                                                   aaaaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATc                                           >  1:948487/1‑100 (MQ=255)
                                                     aaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAg                                         >  2:278334/1‑100 (MQ=255)
                                                      aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                        <  2:767279/100‑1 (MQ=255)
                                                       tCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGt                                       <  2:653654/100‑1 (MQ=255)
                                                       tCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGt                                       <  1:943692/100‑1 (MQ=255)
                                                        ccATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGtt                                      <  1:80925/100‑1 (MQ=255)
                                                           tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTAtn                                   <  1:1469529/100‑2 (MQ=255)
                                                           tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATg                                   <  2:338191/100‑1 (MQ=255)
                                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                  <  2:544931/100‑1 (MQ=255)
                                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                  >  1:1735169/1‑100 (MQ=255)
                                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCCGGTTATg                                   >  2:689656/1‑99 (MQ=255)
                                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                  <  1:1302330/100‑1 (MQ=255)
                                                             tGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGa                                 >  2:754085/1‑100 (MQ=255)
                                                              gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                                >  2:1015252/1‑100 (MQ=255)
                                                              gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                                <  2:948101/100‑1 (MQ=255)
                                                               ccAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACg                               >  2:545895/1‑100 (MQ=255)
                                                                cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACgg                              >  1:1520481/1‑100 (MQ=255)
                                                                   agGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCGGGTTATGGGCGGt                             >  1:1646824/1‑98 (MQ=255)
                                                                      cGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCACGGCGGGCCATCAGGTTATGGACGGTGCTGa                        >  2:689541/1‑100 (MQ=255)
                                                                            ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                  >  2:977949/1‑100 (MQ=255)
                                                                            ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                  >  2:982552/1‑100 (MQ=255)
                                                                            ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                  >  1:859629/1‑100 (MQ=255)
                                                                             gcgcGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggg                 <  1:196282/100‑1 (MQ=255)
                                                                              cgcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGc                <  1:615647/100‑1 (MQ=255)
                                                                               gcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggg                 <  1:1658884/98‑1 (MQ=255)
                                                                               gcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCa               <  1:1467822/100‑1 (MQ=255)
                                                                                cgtcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCAt              >  1:825459/1‑100 (MQ=255)
                                                                                   cgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgt           <  2:165946/100‑1 (MQ=255)
                                                                                    gtgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg          <  1:689739/100‑1 (MQ=255)
                                                                                     tgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtgt         <  2:1710686/100‑1 (MQ=255)
                                                                                     tgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtgt         >  2:1286134/1‑100 (MQ=255)
                                                                                     tgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtgt         >  1:248797/1‑100 (MQ=255)
                                                                                      gtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGtgtg        >  2:185827/1‑100 (MQ=255)
                                                                                      gtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGtgtg        >  2:392877/1‑100 (MQ=255)
                                                                                      gtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGtgtg        >  1:212697/1‑100 (MQ=255)
                                                                                         cGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc     >  1:618207/1‑100 (MQ=255)
                                                                                         cGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc     >  2:98247/1‑100 (MQ=255)
                                                                                          gAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc    <  1:727955/100‑1 (MQ=255)
                                                                                          gAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc    >  1:1383130/1‑100 (MQ=255)
                                                                                            aCCAGCCGGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTg  <  1:1540598/100‑1 (MQ=255)
                                                                                            aCCAGCCCGTGGCGGGAATTCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTg  >  2:1070822/1‑100 (MQ=255)
                                                                                               |                                                                                                
CAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTG  >  NC_000913/281486‑281677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑