Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,279,370 G→T A298S (GCA→TCA)  ghxP → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,3700GT98.7% 262.2 / ‑3.5 76A298S (GCA→TCA) ghxPguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (34/41);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCG  >  NC_000913/4279273‑4279465
                                                                                                 |                                                                                               
gatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATca                                                                                               >  1:1160177/1‑100 (MQ=255)
 atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                              <  1:556681/100‑1 (MQ=255)
 atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                              <  1:1682612/100‑1 (MQ=255)
   gaCGTCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactg                                                                                            <  1:732489/100‑1 (MQ=255)
      ggCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgacc                                                                                         <  2:1916641/100‑1 (MQ=255)
      ggCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgacc                                                                                         <  2:1571692/100‑1 (MQ=255)
       gCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgacca                                                                                        >  2:483414/1‑100 (MQ=255)
        cAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccac                                                                                       >  1:921616/1‑100 (MQ=255)
          gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccactg                                                                                     <  1:1878131/100‑1 (MQ=255)
          gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccactg                                                                                     <  2:309458/100‑1 (MQ=255)
          gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactg                                                                                     >  1:408484/1‑100 (MQ=255)
              ttGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTc                                                                                 <  2:1088993/100‑1 (MQ=255)
               tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTcc                                                                                >  2:1249311/1‑100 (MQ=255)
                 aTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCAt                                                                              <  1:1839333/100‑1 (MQ=255)
                  tGCCACCGGAACTGTCCGTGCCGTCGCCGGCCAAGCGAGTCTGCTGGATAAAGATGTGTAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                             <  1:754155/100‑1 (MQ=37)
                  tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                             <  2:1467127/100‑1 (MQ=255)
                  tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                             <  2:1160179/100‑1 (MQ=255)
                  tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                             >  1:1744705/1‑100 (MQ=255)
                   gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGa                                                                            >  2:1564834/1‑100 (MQ=255)
                   gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGa                                                                            <  2:845134/100‑1 (MQ=255)
                   gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGa                                                                            >  1:1104005/1‑100 (MQ=255)
                    ccACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGag                                                                           <  2:231101/100‑1 (MQ=255)
                     cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagc                                                                          <  1:483412/100‑1 (MQ=255)
                     cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagc                                                                          <  1:503453/100‑1 (MQ=255)
                        cGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagcagc                                                                       >  1:1294471/1‑100 (MQ=255)
                         ggAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCg                                                                      <  1:1721984/100‑1 (MQ=255)
                          gAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGt                                                                     >  1:1387249/1‑100 (MQ=255)
                              tATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtc                                                                 <  1:1401905/100‑1 (MQ=255)
                              tATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtc                                                                 <  2:436420/100‑1 (MQ=255)
                                 ccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTctct                                                              >  2:1388907/1‑100 (MQ=255)
                                  cGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                                                             <  1:1799103/100‑1 (MQ=255)
                                          cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtg                                                     >  2:1799202/1‑100 (MQ=255)
                                          cGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtg                                                     <  1:973367/100‑1 (MQ=255)
                                          cGCCGGCCAGGCGAACCTGCAGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtg                                                     >  2:1615751/1‑100 (MQ=255)
                                              ggCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg                                                 >  2:142646/1‑100 (MQ=255)
                                               gCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGc                                                >  2:317315/1‑100 (MQ=255)
                                                ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                               <  2:55217/100‑1 (MQ=255)
                                                ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                               <  2:1555030/100‑1 (MQ=255)
                                                ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                               <  2:1555130/100‑1 (MQ=255)
                                                  aGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGc                                             <  1:1912792/100‑1 (MQ=255)
                                                      gAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCg                                         <  2:1800806/100‑1 (MQ=255)
                                                      gAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCg                                         <  2:783625/100‑1 (MQ=255)
                                                      gAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCg                                         <  1:1026745/100‑1 (MQ=255)
                                                        aCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGc                                       >  2:377791/1‑100 (MQ=255)
                                                        aCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGc                                       >  1:284082/1‑100 (MQ=255)
                                                           tgctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCGTGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGc                                    >  1:1084855/1‑100 (MQ=255)
                                                           tgctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGc                                    <  1:300862/100‑1 (MQ=255)
                                                           tgctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGc                                    <  2:1677000/100‑1 (MQ=255)
                                                             ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                  <  2:660858/100‑1 (MQ=255)
                                                             ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                  <  2:921617/100‑1 (MQ=255)
                                                              tgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGt                                 >  1:1107132/1‑100 (MQ=255)
                                                              tgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGt                                 <  2:28491/100‑1 (MQ=255)
                                                                gATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtat                               >  1:1456659/1‑100 (MQ=255)
                                                                 aTAAAGATGGGCAGATCATCAACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtata                              <  2:922211/100‑1 (MQ=255)
                                                                  tAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATAc                             >  2:1041441/1‑100 (MQ=255)
                                                                   aaaGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACa                            >  2:1552565/1‑100 (MQ=255)
                                                                    aaGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACAt                           >  1:1078979/1‑100 (MQ=255)
                                                                    aaGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACAt                           <  1:1552555/100‑1 (MQ=255)
                                                                      gATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCg                         <  2:1676649/100‑1 (MQ=255)
                                                                      gATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCg                         >  1:845634/1‑100 (MQ=255)
                                                                         gggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGatt                      >  2:773787/1‑98 (MQ=255)
                                                                         gggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGt                      <  2:1525734/100‑1 (MQ=255)
                                                                         gggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGt                      <  2:597800/100‑1 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                     >  1:1302486/1‑100 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                     >  2:1682905/1‑100 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                     >  1:529186/1‑100 (MQ=255)
                                                                             aGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGGGGGTGCGGCTCCGGCAGCGGTATACATCGAGGCTgc                  >  2:376218/1‑100 (MQ=255)
                                                                                tcatcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggc               <  1:1094682/100‑1 (MQ=255)
                                                                                 natcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg              >  2:1425405/2‑100 (MQ=255)
                                                                                 catcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg              >  2:970275/1‑100 (MQ=255)
                                                                                  atcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGcggcgg             >  2:1702486/1‑100 (MQ=255)
                                                                                  atcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGcggcgg             <  2:1751956/100‑1 (MQ=255)
                                                                                   tcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCggg            <  1:1615741/100‑1 (MQ=255)
                                                                                    cGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTGATGTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCCAGTCTgcggcg              >  1:1809944/1‑97 (MQ=255)
                                                                                          tGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcg     >  2:212825/1‑100 (MQ=255)
                                                                                             gAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcg  >  2:1467882/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCG  >  NC_000913/4279273‑4279465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑