Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,360,814 T→G Y374S (TAC→TCC)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,360,8140TG94.0% 284.0 / 3.7 84Y374S (TAC→TCC) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base G (39/40);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCC  >  NC_000913/4360717‑4360909
                                                                                                 |                                                                                               
gTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAg                                                                                               <  2:228595/100‑1 (MQ=255)
  tATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGt                                                                                             >  1:1056986/1‑100 (MQ=255)
  tATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGt                                                                                             <  1:634723/100‑1 (MQ=255)
   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTa                                                                                            >  2:811278/1‑100 (MQ=255)
     cTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAgctca                                                                                           >  2:610781/1‑95 (MQ=255)
     cTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaaa                                                                                          >  2:1192057/1‑100 (MQ=255)
     cTATTTCTGTGTGCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAGTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaaa                                                                                          <  1:820910/100‑1 (MQ=255)
      tATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAt                                                                                         >  2:439053/1‑100 (MQ=255)
       aTTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAtt                                                                                        <  2:1688940/100‑1 (MQ=255)
        tttCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTc                                                                                       >  1:1654680/1‑100 (MQ=255)
           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                    >  1:1105508/1‑100 (MQ=255)
           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                    <  2:1832877/100‑1 (MQ=255)
           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                    <  2:690683/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                   <  1:693527/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                   <  2:1886668/100‑1 (MQ=255)
             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGTGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                  <  1:353927/100‑1 (MQ=255)
             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                  >  1:1419535/1‑100 (MQ=255)
             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                  <  2:1547742/100‑1 (MQ=255)
               gtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgat                                                                                <  1:1359056/100‑1 (MQ=255)
               gtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgat                                                                                >  2:96563/1‑100 (MQ=255)
               gtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgat                                                                                >  1:454052/1‑100 (MQ=255)
                   aGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGAtt                                                                            <  2:1419543/100‑1 (MQ=255)
                    gTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTg                                                                           <  2:893865/100‑1 (MQ=255)
                      gctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGaa                                                                         >  2:1896914/1‑100 (MQ=255)
                        tgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACa                                                                       <  1:1656338/100‑1 (MQ=255)
                         gctCATTGTTTTTCATCTAAAGGATGTTGAGACTGGCGCACGATATTAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTTTGGGGATGATTGAACa                                                                       >  1:589928/1‑99 (MQ=255)
                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtn                                                                    <  1:1928449/100‑2 (MQ=255)
                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtc                                                                    >  2:462300/1‑100 (MQ=255)
                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtc                                                                    <  2:1322282/100‑1 (MQ=255)
                            aaTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtct                                                                   >  2:1346289/1‑100 (MQ=255)
                            aaTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtct                                                                   >  1:392669/1‑100 (MQ=255)
                            aaTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtct                                                                   >  1:360229/1‑100 (MQ=255)
                              tagTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCa                                                                 >  2:1315689/3‑100 (MQ=255)
                               tGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAc                                                                >  2:983690/1‑100 (MQ=255)
                                gTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAcc                                                               <  2:202850/100‑1 (MQ=255)
                                 tttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCt                                                              >  1:619121/1‑100 (MQ=255)
                                    ttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaat                                                           <  2:878613/100‑1 (MQ=255)
                                    ttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaat                                                           <  1:1119981/100‑1 (MQ=255)
                                     tCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaata                                                          >  2:882939/1‑100 (MQ=255)
                                       aTCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTaataat                                                        <  1:858125/100‑1 (MQ=255)
                                       aTCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTaataat                                                        >  2:185390/1‑100 (MQ=255)
                                        tCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAAtt                                                       >  2:1414328/1‑100 (MQ=255)
                                         cTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTc                                                      <  2:1736225/100‑1 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCa                                                     >  2:1588593/1‑100 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCa                                                     >  2:1169809/1‑100 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTAGCGGAGGTAAATTCTGGGGATGATTGAACATTCTCACCTaataat                                                        >  1:953446/1‑97 (MQ=255)
                                           aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                    >  1:354679/1‑100 (MQ=255)
                                             aGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTg                                                  >  1:1374552/1‑100 (MQ=255)
                                              ggATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACt                                                   <  1:1351721/98‑1 (MQ=255)
                                                 tGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCAc                                              <  1:1860106/100‑1 (MQ=255)
                                                    tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACgg                                            >  2:628604/1‑99 (MQ=255)
                                                     gagaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGtt                                          <  2:619122/100‑1 (MQ=255)
                                                      agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                         >  2:232906/1‑100 (MQ=255)
                                                      agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                         >  1:940012/1‑100 (MQ=255)
                                                       gaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGTACAATCTCACCTAATAATTCACTGGCACGGTTCa                                        >  2:1301377/1‑100 (MQ=255)
                                                         aTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGATGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAAt                                      <  1:1365661/100‑1 (MQ=255)
                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                     <  1:1531891/100‑1 (MQ=255)
                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                     >  1:127799/1‑100 (MQ=255)
                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                     <  2:56947/100‑1 (MQ=255)
                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                     <  1:313955/100‑1 (MQ=255)
                                                           ggCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGa                                    >  2:1167294/1‑100 (MQ=255)
                                                             cgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAtt                                  <  1:1192055/100‑1 (MQ=255)
                                                              gcACGATATCCACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGCTTGAACAATATCACCTAATAATTCACTGGCACGGTTCAATGAttt                                 >  2:395704/1‑100 (MQ=255)
                                                               cACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTa                                <  2:1648370/100‑1 (MQ=255)
                                                               cACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTa                                >  2:1637702/1‑100 (MQ=255)
                                                                   atatCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatcat                            >  1:1056289/1‑100 (MQ=255)
                                                                       cAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGcc                        <  2:1105509/100‑1 (MQ=255)
                                                                       cAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGcc                        >  1:343726/1‑100 (MQ=255)
                                                                       cAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGcc                        >  1:358047/1‑100 (MQ=255)
                                                                         aCTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCAt                      <  2:1876562/100‑1 (MQ=255)
                                                                          cTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATg                     <  1:439051/100‑1 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                    <  2:1567190/100‑1 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                    <  2:839714/100‑1 (MQ=255)
                                                                                cTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaa               >  2:291962/1‑100 (MQ=255)
                                                                                cTTTTTCTGCTCTCGCGGAGGGAAATTCTGGGTATGATTGAACAATCTCACCTAATAATTCACTGTCATGGTTCAATGATTTATCATCGCCATGCAGTaa               >  1:1649845/1‑100 (MQ=255)
                                                                                 tttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaaa              >  1:1013360/1‑100 (MQ=255)
                                                                                   tttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaata            <  2:351188/100‑1 (MQ=255)
                                                                                     tCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaataat          <  2:439987/100‑1 (MQ=255)
                                                                                      cTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATc         >  1:1892365/1‑100 (MQ=255)
                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGGTTTATCATCGCCATGCAGTAAATAATCa        <  1:953745/100‑1 (MQ=255)
                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa        <  2:360231/100‑1 (MQ=255)
                                                                                           ctcGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtgtg    <  1:1610825/100‑1 (MQ=255)
                                                                                            tcGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGc   >  1:789800/1‑100 (MQ=255)
                                                                                             cgcgGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGcc  <  2:1056987/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCC  >  NC_000913/4360717‑4360909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: