Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,503,627 A→G intergenic (‑196/‑431) insG ← / → yjhB IS4 transposase/putative MFS transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,503,6270AG80.0% 224.6 / 50.1 107intergenic (‑196/‑431)insG/yjhBIS4 transposase/putative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base A (13/8);  new base G (47/37);  total (62/45)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01

Only 100 of 110 total aligned reads displayed.

TGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCAT  >  NC_000913/4503529‑4503723
                                                                                                  |                                                                                                
tgtTTGTAACAAAAAAAAAGATAAAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGta                                                                                                  <  1:799249/99‑1 (MQ=255)
 gtTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGtgta                                                                                                <  1:528254/100‑1 (MQ=255)
 gtTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGtat                                                                                                 <  1:567197/99‑1 (MQ=255)
   ttGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAAc                                                                                              >  1:907600/1‑100 (MQ=255)
     gTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAg                                                                                            <  2:1451693/100‑1 (MQ=255)
      tAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAgg                                                                                           <  2:955712/100‑1 (MQ=255)
      tAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAgg                                                                                           <  2:1073657/100‑1 (MQ=255)
      tAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAga                                                                                           <  2:1253767/100‑2 (MQ=255)
       aaCAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGt                                                                                          >  1:870814/1‑100 (MQ=255)
        aCAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGta                                                                                         <  1:15630/100‑1 (MQ=255)
         cAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGtat                                                                                        >  2:649947/1‑100 (MQ=255)
         cAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGtat                                                                                        >  1:152479/1‑100 (MQ=255)
              tATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAgg                                                                                   <  1:438360/100‑1 (MQ=255)
              tATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAgg                                                                                   <  1:438559/100‑1 (MQ=255)
               aTCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGt                                                                                  <  2:15013/100‑1 (MQ=255)
               aTCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGt                                                                                  >  2:465236/1‑100 (MQ=255)
                tCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGta                                                                                 <  2:167691/100‑1 (MQ=255)
                tCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGta                                                                                 <  1:460539/100‑1 (MQ=255)
                 cAGCTACAAAAATATGCTCAATGTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGtat                                                                                >  1:1132553/1‑100 (MQ=255)
                     tACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTTTTTTTTTTTAACAGGTATAAAGGTATACa                                                                             >  1:1320930/1‑99 (MQ=255)
                      aCAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGa                                                                           >  2:233035/1‑100 (MQ=255)
                       cAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTGTGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGaa                                                                          >  2:1791738/1‑100 (MQ=255)
                       cAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGaa                                                                          >  1:1888452/1‑100 (MQ=255)
                       cAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGaa                                                                          >  1:1889914/1‑100 (MQ=255)
                       cAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGaa                                                                          >  1:1942013/1‑100 (MQ=255)
                        aaaaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGaaa                                                                         <  2:325404/100‑1 (MQ=255)
                          aaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAg                                                                       <  2:762026/100‑1 (MQ=255)
                          aaaTATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAg                                                                       <  2:1918903/100‑1 (MQ=255)
                                gCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGa                                                                 >  1:157618/1‑100 (MQ=255)
                                 cTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaa                                                                >  2:1710683/1‑100 (MQ=255)
                                  tCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGaaa                                                               >  2:1317161/1‑100 (MQ=255)
                                  tCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGaaa                                                               <  1:909608/100‑1 (MQ=255)
                                   cAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAACGCAAAGAAAt                                                              >  1:1272867/1‑100 (MQ=255)
                                      tttGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACt                                                           >  1:1156535/1‑100 (MQ=255)
                                        tgtgACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGc                                                         >  2:1430258/1‑100 (MQ=255)
                                          tgACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCaa                                                       >  1:930851/1‑100 (MQ=255)
                                            aCATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAg                                                     >  2:1494501/1‑100 (MQ=255)
                                              aTCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGa                                                   <  2:907601/100‑1 (MQ=255)
                                              aTCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGa                                                   <  2:515737/100‑1 (MQ=255)
                                               tCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaa                                                  >  1:1360647/1‑100 (MQ=255)
                                               tCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaa                                                  >  1:1507263/1‑100 (MQ=255)
                                               ctaGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaa                                                  <  1:1688308/98‑1 (MQ=255)
                                               cacatAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaa                                                  >  2:1882230/5‑100 (MQ=255)
                                                 aGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaaag                                                >  2:1688318/1‑99 (MQ=255)
                                                 aGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaaaa                                                <  2:1942026/100‑1 (MQ=255)
                                                 aGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGaaaa                                                >  1:38267/1‑100 (MQ=255)
                                                  gTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTTGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAAc                                               >  1:49250/1‑100 (MQ=255)
                                                  gTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAAc                                               <  1:1718219/100‑1 (MQ=255)
                                                  gTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAAc                                               <  2:1507271/100‑1 (MQ=255)
                                                   tAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACa                                              >  1:829989/1‑100 (MQ=255)
                                                     aCAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGc                                            >  1:1731079/1‑100 (MQ=255)
                                                     aCAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGc                                            <  1:233033/100‑1 (MQ=255)
                                                      cAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGc                                            >  1:1664694/1‑99 (MQ=255)
                                                      cAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCt                                           >  2:1045112/1‑100 (MQ=255)
                                                      cAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCt                                           <  2:1451096/100‑1 (MQ=255)
                                                         aaCGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCtata                                        <  1:1655797/100‑1 (MQ=255)
                                                           cgcgTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAAc                                      >  1:804373/1‑100 (MQ=255)
                                                            gcgTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACg                                     <  1:1217348/100‑1 (MQ=255)
                                                             cgTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGt                                    >  1:455048/1‑100 (MQ=255)
                                                              gTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTa                                   >  1:1480660/1‑100 (MQ=255)
                                                              gTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTa                                   <  2:1546686/100‑1 (MQ=255)
                                                              gTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTa                                   >  1:933332/1‑100 (MQ=255)
                                                              gTTTTGTTATGTAGACTGCTTGTTTTATGATCTATTGTAACAGCTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAACGAAAACAGCTATAACGt                                    <  1:1349720/99‑1 (MQ=255)
                                                               ttttGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTaa                                  <  1:1011873/100‑1 (MQ=255)
                                                                tttGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAg                                 <  2:1272872/100‑1 (MQ=255)
                                                                  tGTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCt                               >  1:781913/1‑100 (MQ=255)
                                                                   gTTATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTGTAACGTAAGCTa                              >  1:636944/1‑100 (MQ=255)
                                                                    ttATGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTaa                             <  2:1543936/100‑1 (MQ=255)
                                                                      aTGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAg                           <  1:82973/100‑1 (MQ=255)
                                                                      aTGTGGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAg                           >  1:869869/1‑100 (MQ=255)
                                                                       tgtgGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGt                          <  1:1710672/100‑1 (MQ=255)
                                                                       cgtgGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGt                          >  2:1379574/2‑100 (MQ=255)
                                                                        gtgGATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGta                         >  2:348844/1‑100 (MQ=255)
                                                                         tgGATTGCTTGTTTTTTTTTTTTTTTTTACAGGTATAAAGGTATACAGAAAAGCAAAGTAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaa                        >  1:1053887/1‑100 (MQ=255)
                                                                          ggATTGCTTGTTTTTTGATCTGGTGTAACAGGTATAGAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGtaat                       >  2:1369218/1‑100 (MQ=255)
                                                                             ttGCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAAc                    >  1:1402917/1‑100 (MQ=255)
                                                                              tggttGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAAcc                   <  2:297365/97‑1 (MQ=255)
                                                                               gCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACct                  >  2:1292777/1‑100 (MQ=255)
                                                                               gCTTGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACct                  >  2:1408098/1‑100 (MQ=255)
                                                                                  tGTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCtctc               >  2:158025/1‑100 (MQ=255)
                                                                                   gTTTTTTGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCa              >  2:664021/1‑100 (MQ=255)
                                                                                   gTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCa              <  1:1353338/100‑1 (MQ=255)
                                                                                    ttttttGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTGTAACGTAAGCTAAAGTAATAACCTCTCAg             <  2:636945/100‑1 (MQ=255)
                                                                                    ttttttGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAg             >  2:423835/1‑100 (MQ=255)
                                                                                    ttttttGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAg             >  2:1716434/1‑100 (MQ=255)
                                                                                    ttttttGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAg             <  1:844878/100‑1 (MQ=255)
                                                                                     tttttGATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGt            >  2:1323924/1‑100 (MQ=255)
                                                                                          gATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTc       <  1:517389/100‑1 (MQ=255)
                                                                                          gATCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTc       >  2:1675332/1‑100 (MQ=255)
                                                                                           aTCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTcc      <  2:554846/100‑1 (MQ=255)
                                                                                           aTCTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTcc      <  2:1394649/100‑1 (MQ=255)
                                                                                             cTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTc    >  2:189152/1‑100 (MQ=255)
                                                                                             cTGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTc    >  2:1164113/1‑100 (MQ=255)
                                                                                             cTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTc    >  1:1393633/1‑100 (MQ=255)
                                                                                              tGGTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCa   >  2:1809972/1‑100 (MQ=255)
                                                                                               ggTGTAACAGGTATAAAGGTATACAGAAAAGCAGAGAAATACGGCAAAGGAAAACAGCTATAACGTAAGCTAATGTAATAACCTCTCAGTCTTTCCTCAt  >  1:1050388/1‑100 (MQ=255)
                                                                                               ggTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCAt  >  1:1360969/1‑100 (MQ=255)
                                                                                               ggTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCAt  >  1:151042/1‑100 (MQ=255)
                                                                                               ggTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCAt  >  2:717680/1‑100 (MQ=255)
                                                                                               ggTGTAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCAt  >  2:853083/1‑100 (MQ=255)
                                                                                                  |                                                                                                
TGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGGTATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCAT  >  NC_000913/4503529‑4503723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: